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Citations for Addgene :
451 - 500 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were amplified with PCR and cloned into Ac5-Stable2-neo (Rosa Barrio and James Sutherland60, Addgene 32426) with inserts removed by PCR.
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel-purified and cloned into EcoRV-cut pLenti CMV Puro DEST (w118-1) (Addgene #17452 ...
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bioRxiv - Cell Biology 2021Quote: ... The miRFP670 was amplified by PCR from pmiRFP670-N1 (a gift from Vladislav Verkhusha, Addgene plasmid #79987) (Shcherbakova et al. ...
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bioRxiv - Genetics 2022Quote: ... as the selection module for all TFT constructs and obtained this sequence by PCR amplification from Addgene plasmid #41030 (a gift from Michael Boddy) ...
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bioRxiv - Cancer Biology 2022Quote: ... After PCR the insert was gel purified (GeneJet) and Gibson cloned into BsmBI-digested pXPR_053 (Addgene# 113591). Gibson cloned insert and vector was column purified (GeneJet ...
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bioRxiv - Bioengineering 2022Quote: ... another fragment coding sfGFP was PCR-amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (#60904, Addgene) using oKK334 and oKK335 ...
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bioRxiv - Developmental Biology 2022Quote: ... EF1alpha-GCamp6m lentiviral vector was generated by PCR amplification of GCamp6m from pGP-CMV-GCaMP6m (Addgene #40754) using with Q5 High Fidelity master mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... the doxycycline inducible Tet-On-3G_hPGK promoter sequence was PCR amplified from the plasmid pMK243 (Addgene, #72835) with 5’ SalI and 3’ MluI digestion sites ...
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bioRxiv - Cell Biology 2022Quote: ... The BG4 sequence was amplified by PCR from the pSANG10-3F-BG4 plasmid (Addgene plasmid # 55756(12)) while the mitochondrial targeting sequence (MTS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We PCR amplified LexApr using oligos 1195 and 1240 from FRP1642 (Addgene #58442, Ottoz et al. 2014). We PCR amplified CYC1 terminator from pSZB1040 using oligos 2194 and 2195 ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared by PCR and transferred to a retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
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bioRxiv - Neuroscience 2021Quote: ... The low affinity glutamate sensor iGluSnFR S72A was PCR amplified from the pAAV.hSynapsin.SF-iGluSnFR.S72A which was a gift from Loren Looger (Addgene plasmid # 106176 ...
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bioRxiv - Developmental Biology 2020Quote: 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019). Guide RNAs and the donor vector were co-injected into vas-Cas9 embryos (BDSC #51324 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2500 ng of purified HDR PCR product and 1500 ng of guide RNA expression plasmid (Addgene 49330) containing the relevant guide RNAs (Supplementary Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... PTEN coding sequence was PCR amplified from cDNA and cloned into pLV-EF1a-IRES-Neo (Addgene #85139) (80 ...
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bioRxiv - Plant Biology 2023Quote: ... NJ) or amplified by PCR with overhangs containing SapI recognition sites and assembled into pUAP4 (Addgene #136079) to create parts compatible with the Phytobrick standard (74) ...
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bioRxiv - Molecular Biology 2023Quote: ... template libraries were constructed by PCR sewing and cloned into pRSII413 (a gift from Steven Haase, Addgene plasmid #35450 ...
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bioRxiv - Microbiology 2023Quote: ... the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Developmental Biology 2022Quote: ... was used for PCR reactions with 30-50 ng of pScarlessHD-DsRed plasmid as template (Addgene #64703) or 50 ng of gBlocks or genomic DNA from the same Drosophila strain (BDSC #55821) ...
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bioRxiv - Biochemistry 2022Quote: ... Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386 ...
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bioRxiv - Genetics 2022Quote: ... 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019) [73] ...
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bioRxiv - Synthetic Biology 2022Quote: ... It was synthesised by PCR amplifying the αGCN4 backbone from LLP252 pEF1a-NLS-scFvGCN4-DNMT3a (Addgene, #100941) and the first 639 bp of the 5’ end of p65HSF1 from pAC1393-pmax-NLSPUFa_p65HSF1 (Addgene ...
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bioRxiv - Synthetic Biology 2022Quote: ... and CAS9 was inserted via Gibson Assembly after PCR via Cr_1250 and Cr_1254 from pCRISPRyl (Addgene #70007). Integration was accomplished as previously described using a two plasmid CRISPR-mediated markerless approach 44 ...
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bioRxiv - Cell Biology 2023Quote: ... the AGO2 CDS was PCR-amplified using pFRT/FLAG/HA-DEST EIF2C2 plasmid (Addgene: Cat. # 19888[57]) as template and Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... we generated PCR amplified EF1a promoter and TetR-NLS signal cassette from pLV-tTRKRAB-red (Addgene, #12250). 3xFlag-KRAB was PCR amplified from the same vector with a forward primer including the sequence encoding 3xFlag and SV40pA amplified from lentiCRIPSRv2 ...
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bioRxiv - Genetics 2023Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
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bioRxiv - Genetics 2023Quote: To replace nvYFP with mCherry we PCR amplified mCherry from pQC NLS mCherry IX (Addgene plasmid 37354) using primers #1833 and #1834 and PCR amplified attB-Dfd-3XP3 from pAttB-Dfd-3XP3-nvYfp with primers #1831 and #1832 and performed Gibson assembly to generate plasmid #795 = pAttB-Dfd-3xP3-mCherry.
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bioRxiv - Developmental Biology 2023Quote: ... we added the T7 promoter sequence to the sgRNA template by PCR amplification of px330 (Addgene, 42230) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... human RXRa cDNA was PCR-amplified from pSV-Sport-RXRα (a gift from Bruce Spiegelman; Addgene #8882)68 ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR cassette was transfected into HeLa cells with a helper plasmid containing AsCas12a endonuclease (Addgene 89353). Following cleavage ...
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bioRxiv - Molecular Biology 2024Quote: ... amplified by PCR and cloned into the NotI and AflII sites of the pEN366 backbone (Addgene #156432). All of the constructs were verified by DNA sequence analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... pVP076 was also digested and assembled via NEBuilder with a PCR amplicon containing mRFP1E chromoprotein (Addgene #160442) in place of ccdB/CmR ...
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bioRxiv - Bioengineering 2024Quote: ... the MS2 coat protein (MCP) (N55K variant) ORF was PCR amplified from MS2-P65-HSF1_GFP (Addgene, 61423)74 and inserted with a serine-glycine linker via Gibson Assembly into pLV_CAG_Gag at the C-terminus of MLV Gag in order to generate the plasmid pLV_CAG_Gag-MCP ...
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bioRxiv - Biophysics 2024Quote: ... CyOFP1 and mSca gene fragments were PCR amplified from pLSSmKate2-C1 (a gift from Vladislav Verkhusha; Addgene plasmid # 31869 ...
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bioRxiv - Cell Biology 2024Quote: ... H2B-miRFP was generated by PCR from pH2B- miRFP703 (a gift from Vladislav Verkhusha, Addgene plasmid # 80001) and ligation into pLVXEneo (pLVXEblast with blastR replaced with neoR ...
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bioRxiv - Cancer Biology 2024Quote: ... which were placed in tandem using Gibson assembly into a PCR linearized Lenti-Cre vector (ref; RRID:Addgene_67594).
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bioRxiv - Neuroscience 2020Quote: ... 1:5 dilution in saline solution) or AAV1.CAG.DIO.tdTomato (control, 120 nl, 2.6×1013 vg/mL Addgene, diluted 1:5 in saline) was injected into AL ...
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bioRxiv - Cancer Biology 2022Quote: ... A solution of retroviral DNA plasmids (TRI-dsRED-IDH1R132H, MSCV-GFP-DNMT3AR882H, MSCV-tTA-NrasG12D, pclEco packaging vector (Addgene plasmid cat# 12371), 0.25 M CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... DNA sequences between nucleotides 1-3877 and 4236-4617 were PCR amplified from V5-GFP-P180 (Addgene #92150) and the two fragments were assembled and cloned into pEGFP(A206K)-N1 between XhoI and BamHI sites by GIBSON assembly ...
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bioRxiv - Genetics 2021Quote: TPM2 mammalian expression constructs were generated by PCR subcloning variants from pUASt.attB into pCMV-IRES-eGFP (Addgene 78264) using XbaI/EcoR1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then cloned into the pPD95.77 vector (Fire Lab C. elegans vector kit; Addgene, Cambridge, MA) via the SalI and BamHI sites ...
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bioRxiv - Neuroscience 2021Quote: hM4D(Gi) was amplified by PCR from pcDNA5/FRT-HA-hM4D(Gi) (Addgene #45548 (Armbruster et al., 2007)) using forward primer ...
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bioRxiv - Neuroscience 2021Quote: Kir was amplified by PCR from pCAG-Myc-Kir2.1E224G/Y242F-2A-EGFP (similar to pCAG-Kir2.1-T2A-tdTomato, Addgene #60598 ...
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bioRxiv - Genetics 2020Quote: ... A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213925 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were then subcloned into the pDEST-ORF-v1 (a kind gift from Darren Saunders, Addgene, #73637) (Croucher et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs, Addgene # 25999) (Beronja et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ; http://n2t.net/addgene:122848; RRID:Addgene_122848) (94 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA sequences of full-length CAD and its fragments were amplified by PCR from pcDNA3.1-HisFlag-CAD (Addgene) and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences ...