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Citations for Addgene :
1801 - 1850 of 1882 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: To measure mitophagy in HUVEC were seeded in 6-well plates (8 x 105 cells/well) and transfected with 5ug/well of pCHAC-mt-mkeima plasmids (Addgene plasmids #72342) at a ratio of 1:1.5 plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-MARCO-CHO and GFP-MARCO-HEK cells were further transduced with RFP-LC3 plasmid which was a gift from Tamotsu Yoshimori (Addgene, plasmid #21075) (Kimura et al ...
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bioRxiv - Systems Biology 2023Quote: ... ura3 his3 met15 leu2::GEM gal4::NAT gal1::stuffer-linker-GFP-HIS3) competent cells were co-transformed with a pCAS plasmid (Addgene plasmid 6084747) expressing both the gRNA for the stuffer (Dionne et al 2021 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were expanded and transfected (day 0) with PEI at a coverage of > 500x with pCMV-PE2-tagRFP-BleoR (Addgene no. 192508). One day later ...
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bioRxiv - Cell Biology 2023Quote: Stable MEL cell clones expressing dCAS9-KRAB were generated using Lenti-dCAS9-KRAB-blast (Gift from Dr. Gary Hon, Addgene plasmid #89567). 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress ...
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bioRxiv - Developmental Biology 2023Quote: HEK293T cells were plated at a density of 2.8×105 cells in 6-well plates and transfected with MSCV-flag-PRDM16 (Addgene, 15504; RRID:MSCV PRDM16) and/or pCDNA3-NKX2-174 using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells using the plasmids pRSFDuet-1 His6-SUMO-CoiA1_CoiB and pETDuet-1 Coi_CoiSA(ED) (Addgene ID #208761 and #208762), following the method previously reported.10 For the preparation of methyllanthionine standard ...
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bioRxiv - Cell Biology 2023Quote: Donor plasmid encoding homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1 was designed by the Allen Institute for Cell Science and obtained from Addgene (AICSDP-50). The plasmid encoding NPM1-mEGFP was a gift from the Allen Institute for Cell Science (Addgene plasmid # 109122) ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were transfected with the engineered pSBtet-BP::eGFP-PLCδ-PH and pCMV(CAT)T7-SB100 plasmid (Addgene, Plasmid #34879) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were triple transfected with lentivirus packaging plasmid psPAX2 and the envelope plasmid pCMV-VSV-G (Addgene plasmids #12260 and #8454) in addition to the transgene of interest using FuGene HD (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... the cells were co-transfected with two plasmids: pAS_4xU6-PyIT M15(UUA) FLAG-Mma PyIRS (Mma PylT/RS) from Addgene (Watertown, MA, #154774)25 and the Acd RS1 tRNA/aminoacyl-tRNA synthetase pair (GCE4All Research Center ...
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bioRxiv - Microbiology 2024Quote: ... Oligos encoding sgRNAs for generating knockout cells using CRISPR–Cas9 were cloned into the lentiCRISPRv2 plasmid (a gift from F. Zhang, Addgene plasmid 52961) as previously described [66] ...
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bioRxiv - Cell Biology 2024Quote: ... the CAAX domain of K-ras tagged with mTagRFP-T was introduced into a safe harbor locus via CRISPR using a donor plasmid developed at the Allen Institute for Cell Science and sourced from Addgene (Plasmid #107580a). We call the resulting cell line CT20 ...
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bioRxiv - Molecular Biology 2024Quote: ... The ppyCAG_RNaseH1_WT employed for the transient overexpression of RNAseH1 in vitro on HEPA1-6 cells was a gift from Xiang-Dong Fu (Addgene plasmid #111906).
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bioRxiv - Biochemistry 2022Quote: ... These GFP positive cells were transduced with lentiviral particles for pLenti CMV rtTA3 Hygro (w785-1) (a gift from Eric Campeau Addgene plasmid number 26730) for tetracycline inducible expression and selected using hygromycin (200μg/ml) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293T cells were transfected with psPAX2 and pMDG.2 packaging plasmids (gifts from Didier Trono, EPFL, Lausanne, Switzerland; Addgene plasmids 12559 and 12660) and the lentiviral expression construct ...
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bioRxiv - Developmental Biology 2021Quote: ... AAVS1 repair template was created by Infusion cloning to swap the CAG promoter and Puromycin resistance cassette in plasmid AICSDP-42: AAVS1-mTagRFPT-CAAX (a gift from The Allen Institute for Cell Science, Addgene plasmid # 107580). SOX2 knockout repair template was created by Infusion cloning to insert SOX2 5’ and 3’ homologous arms in EasyFusion T2A-H2B-GFP (a gift from Janet Rossant ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines stably expressing the Cas9 nuclease were generated by infection with the empty lentiCRISPRv2 lentivirus (Addgene, #52961, not containing an sgRNA guide) using standard methods (https://www.addgene.org/viral-vectors/lentivirus/lenti-guide/) ...
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bioRxiv - Cancer Biology 2020Quote: pBABE-SAOS 2 and hTERT-SAOS 2 stable cell lines were obtained upon transfection of the SAOS 2 cell line with the plasmids pBABE-puro or pBABE-puro-hTERT from Addgene (#1764, #1771, respectively), and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: Stably Cas9-expressing HepG2 cells were transduced with genome-wide lentiviral pooled CRISPR/Cas9 knockout library (Addgene #73178) (Brunello; Cat# 73178-LV) (Addgene, Inc., Watertown, MA) containing 76,441 sgRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Conditional knockout cells were generated by infection with media from a confluent 10 cm dish of 293T cells transfected with 3 µg pCL-Eco (Addgene, 12371 ref (17)) and 3 µg MSCV CreERT2 puro (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were transfected with psPAX2 and pMDG.2 packaging plasmids (gifts from Didier Trono, EPFL, Lausanne, Switzerland; Addgene plasmids 12559 and 12660) and the lentiviral expression construct ...
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bioRxiv - Molecular Biology 2019Quote: ... 293T cells seeded onto 150mm dishes were transfected with 21 µg of the murine lenti-GeCKO-V2 plasmid library (Addgene pooled library #1000000053), 0.168 ng each of the sgRNAs targeting miR-15mut and miR-16-1mut ...
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bioRxiv - Genomics 2019Quote: 293FT cells were transfected with 3rd generation system lentiviral plasmids (pMDLg/pRRE, pRSV-Rev and pMD2.G; Addgene #12251, 12253 and 12259, respectively) to generate viral particles using the PEIpro reagent (Polyplus-Transfection) ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral particles carrying a luciferase reporter were produced at the EPFL Gene Therapy Facility by transfecting HEK293 cells with the pHIV-Luc-ZsGreen plasmid (Addgene, Catalog No. 39196). Lentivirus-containing supernatants were collected and concentrated by centrifugation (1,500 g for 1 hr at 4°C) ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293T cells were transfected with above lentiCRISPR SHMT2 plasmid or control V2 plasmid together with helper plasmids pPAX2 and UVSVG (Addgene #8454 and #12260) using Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... A549 cells were first made to stably express Streptococcus pyogenes Cas9 following blasticidin selection of cells transduced with lentiCas9-Blast (gift from Feng Zhang, Addgene plasmid # 52962 (19)) ...
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bioRxiv - Microbiology 2020Quote: ... were obtained by transducing wild type HCT116 colorectal carcinoma cell line (ATCC® CCL-247™) with lentiCas9-Blast lentiviral vector (Addgene #52962). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: OE19-dCas9-KRAB stable cells were generated by transfecting 1×106 OE19 cells with 7.5 μg Cas9 plasmid with guides targeting the AAVS1 locus (Addgene #42230; 5’-GGGGCCACTAGGGACAGGAT-3’) and 7.5 μg donor plasmid (pAAVS1-Puro-DNR ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T cells were transfected with psPAX2 and pMDG.2 packaging plasmids (gifts from Didier Trono, EPFL, Lausanne, Switzerland; Addgene plasmids 12559 and 12660), the lentiviral expression plasmid ...
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bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected with targeting plasmid and 3rd generation packing plasmid (pMD2.G, pMDLg/pRRE; Addgene #12251, and pRSV-Rev; Addgene #12253) together using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2023Quote: The pInducer20-NA7-Nef-Halo vector was packaged into lentiviruses in HEK293T cells by co- transfection with 2nd generation lentiviral packaging plasmids (Addgene 182993 and 244397) using TransIT-LT1 transfection reagent according to the manufacturer’s instructions (Mirus 2304) ...
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library set A and B (Addgene, 92379 and 92380) separately ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T cells in 10-cm dishes were transfected at 80% confluence with the lentiviral plasmid pLenti-CMV-Puro (Addgene plasmid no. 17452) containing the gene of interest (2 mg ...
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bioRxiv - Microbiology 2023Quote: ... cells were transduced with the respective lentivirus (pWPI-IRES-Puro-Ak-ACE2, kindly provided by Dr. Sonja Best, Addgene viral prep # 154985-LV) at MOI 0.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were packaged in HEK293T cells (ATCC: CRL-3216™) by co-transfection of pLentiCRISPR v2-β2m-gRNA with pRSV-rev (Addgene Plasmid #12253), pMDLg/pRRE (Addgene Plasmid #12251 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells expressing sgSAFE-mCherry (denoted by an additional superscript ‘C’) were generated from the respected parental cells via transduction with the lentiviral sgRNA expression vector with mCherry pMCB320 (Addgene plasmid no. 89359) virus ...
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bioRxiv - Developmental Biology 2023Quote: ... 100K-200K HEK 293 cells were reverse transfected with the M50 Super 8x TOPFlash plasmid (100 ng; Addgene #12456; gift from Randall Moon) and a construct constitutively expressing Renilla luciferase (5 ng ...
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bioRxiv - Cancer Biology 2023Quote: ... Packaging 293T cells were transfected with KEAP1 single guide RNA (sgRNA) and helper vectors (pMD2.G and psPAX2; Addgene plasmid # 12259 and 12260) using Lipofectamine 3000 reagent (Cat ...
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bioRxiv - Genetics 2023Quote: Lentiviruses were produced by co-transfection of HEK293T cells with the relevant lentiviral transfer vector and packaging vectors psPAX2 (Addgene, catalog no. 12260) and pMD2.G (Addgene ...
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bioRxiv - Genetics 2024Quote: ... Lentivirus was produced from sgRNA plasmids by co-transfecting Lenti-X 293T (Takarabio, 632180) cells with the sgRNA plasmid library and packaging plasmids-psPAX2 and pMD2.G (Addgene, 12260 and 12259) using Trans-IT-293 transfection reagent (Mirus ...
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bioRxiv - Microbiology 2022Quote: ... a total of 300 million A549-Cas9 cells were then transduced with the lentiviral human GeCKO v2 library (Addgene #1000000049, gift from Feng Zhang)(29 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentivirus was produced in HEK293T cells using a 2nd generation lentiviral packaging system (psPAX2 – Addgene plasmid #12260 and pMD2.G – Addgene #12259 from Didier Trono) and pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP (Addgene #32628 from Andrew Scharenberg ...
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bioRxiv - Genetics 2020Quote: ... OCI-AML3 and K562 cells were first transduced with the lentiviral vector pHR-SFFV-KRAB-dCas9-P2A-mCherry (Addgene 60954, deposited by Jonathan Weissman), sorted for mCherry on a FACSAria II ...
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bioRxiv - Immunology 2019Quote: Lentiviruses were packaged in HEK 293T cells using the VSV-G pseudotyping vector pMD2.G and the viral packaging vector psPAX2 (gifts from Didier Trono; Addgene plasmids #12259 and #12260). Transfection mixes were prepared by combining the transfer ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were co-transfected using lentiviral packaging plasmids (psPAX2 and pMD2.G) with a PLKO.1-blast plasmid (Addgene, Cambridge, MA, USA, #26655) containing shSmad2 or shYAP ...
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bioRxiv - Neuroscience 2019Quote: ... Similar experiments were conducted using mammalian PSD-95 protein (prepared by transfecting expression vectors pCMV-PSD95-flag [FLAG-tagged; Addgene, #15463] into HEK293 cells) bound to GluN2B-peptide-containing Agarose resins ...
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bioRxiv - Cell Biology 2021Quote: ... or hTMIGD1 cDNAs were generated by co-transfection of HEK293T cells with the lentiviral vector and the packaging vectors psPAX2 and pMD2.G (kindly provided by Dr Didier Trono, Addgene plasmids 12260 and 12259) in a ratio of 3:2:1 into HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: Lentivirus-derived VLPs were produced by transfecting 10 cm dishes of HEK293T LentiX cells with lentiviral packaging plasmids encoding Gag/Pol (pMDLg/pRRE, 15 µg) and Rev (pRSV-REV, 6 µg) (Addgene plasmids 12251 and 12253) together with vectors expressing SARS-CoV-2 Spike (13 µg ...