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Citations for Addgene :
1751 - 1800 of 1882 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Expression constructs needed to generate biotinylated anti-GFP nanobody for native purification from human cells are available from Addgene (#149336, #149334, #149333) (Pleiner et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Tetracycline-inducible MYH12A WT and S1AS2A-mutant IA32 cell lines were made by first preparing a stable rtTA-expressing IA32 cells using pLenti-CMV-rtTA3-hygro lentivirus infection (virus made with Addgene plasmid 26730) and selection with 100 μg/mL hygromycin ...
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bioRxiv - Microbiology 2021Quote: ... FMNL3-V5 was expressed in U937 FMNL3 KO using lentiviruses generated in 293E cells co-transfected with the packaging constructs psPAX2 (Addgene plasmid 12260) and pCMV-VSV-G (Addgene plasmid 8454).
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bioRxiv - Cancer Biology 2020Quote: ... To knock-out Psat1 in cells isolated from MYC-driven tumours induced in Psat1fl/fl mice the retroviral vector MSCV-CreERT2 puro (Addgene plasmid #22776) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting HeLa-dCas9 cells were then transduced with the lentiviral construct for the MS2-P65-HSF1 (MPH) activator complex (Addgene 61426-LVC) and selected with 0.5 mg/ml hygromycin ...
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bioRxiv - Cancer Biology 2019Quote: The B-cell acute lymphoblastic leukemia cell lines NALM-6 and REH were lentivirally transduced with plasmid expressing Cas9 nuclease (LentiCRISPR V2; Addgene Plasmid #52961) as described below at a MOI of 0.5 ...
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bioRxiv - Microbiology 2021Quote: Cells stably expressing shRNA of different genes under the control of a tetracycline-inducible promoter were created by first transducing SNB-19 cells with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; number 17492). The cells underwent selection with media containing 10 μg/mL of blasticidin (Invivogen ...
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bioRxiv - Cell Biology 2019Quote: ... YFP-NuMA and mEmerald-IFT88 LLC-PK1 cells were generated by transfection of LLC-PK1 with pEYFP-C1-NuMA (Addgene plasmid #28238) or mEmerald-IFT88-N-18 (Addgene plasmid #54125) ...
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bioRxiv - Cell Biology 2019Quote: Constructs used to rescue SHP2 knockout U2OS cells were obtained by cloning the SHP2 cDNA from pCMV-SHP2-WT (Addgene plasmid # 8381) into the migR1-IRES-GFP vector (Addgene plasmid # 27490) ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... iBMDM GFP-TFEB stably transfected cells were created using pEGFP-N1-TFEB (pEGFP-N1-TFEB was a gift from Shawn Ferguson, Addgene plasmid # 38119), Lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Lentivirus production was carried out by co-transfecting HEK293T cells with 12.5 μg of Cas9 plasmid with blasticidin resistance (Addgene, cat. no. 52962), 7.5 μg psPAX2 plasmid and 4 μg the packaging pVsVg plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... HeLaH2B-GFP- and HeLa2FPs-KO-PA28γ cell lines were generated by cotransfection of PSME3/PA28γ sgGuide and pX459 vectors (a gift from Feng Zhang, Addgene plasmid #62988), and cells were selected with puromycin (1 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells plated on polylysine-coated coverslips were transfected with cDNA (100 ng/40000 cells) coding for mitochondrial-targeted mCherry (mCherry-Mito-7, a gift from Michael Davidson (Addgene plasmid # 55102), (Olenych et al ...
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bioRxiv - Immunology 2020Quote: ... Lentiviruses were generated by co-transfecting Lenti-X 293T cells with the lenti-vector and the packaging plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259 ...
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bioRxiv - Immunology 2021Quote: ... DFT cells were co-transfected with pCO2 and SB transposase vector pCMV(CAT)T7-SB10025 (a gift from Zsuzsanna Izsvak; Addgene plasmid # 34879) at a ratio of 3:1 in μg ...
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bioRxiv - Microbiology 2021Quote: ... Lentivirus was produced by triple transfection of 293T cells with the sgRNA expression LentiCRISPR-v2 vector and the packaging plasmids psPAX2 (Addgene plasmid # 12260) and pMD2.G (Addgene plasmid # 12259 ...
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bioRxiv - Molecular Biology 2020Quote: ... The TRIPZ plasmid was transfected into 293T cells along with lentiviral packaging and envelope 2nd generation plasmids (Addgene packaging 11263, Addgene envelope 17576). 48 hours post-transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... Rcho1 cells were stably cotransfected with the vectors (phU6-gRNA) expressing enhancer gRNAs and Cas9 (pLV hUbc-Cas9-T2A-GFP, Addgene, Plasmid #53190)[32] using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... this cell line was co-transfected with a 9:1 ratio of pCENP-B-Cherry (a gift from Michael Lampson; Addgene plasmid #45219) and pPGKpuro resistance plasmid ...
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bioRxiv - Genomics 2021Quote: All gRNAs used for HEK293T cells and mESCs targeting have been cloned into the double BbsI sites of pX330-vector (Addgene ID 42230). The plasmid used for CH12F3 cell targeting was an optimized vector in which we removed the AAV2 ITR sequence and introduced a mCherry gene with CMV promoter by Gibson assembly into pX330 vector.
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bioRxiv - Genetics 2020Quote: ... this cell line was transfected with a plasmid expressing Bxb1 integrase (pCAG–NLS–HA–Bxb1; Addgene #51271, a gift from Pawel Pelczar36) by transient transfection with FuGENE 6 (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... CRISPR-mediated gene disruption was performed by identifying individual gRNA sequences with consistent enrichment in LDLlow cells in our previously reported CRISPR screens [7] and cloning these sequences into the BsmBI sites of pLentiCRISPRv2 (Addgene #52961, [43]) or into the BbsI sites of pX459 (Addgene #62988 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated using 293FT cells by co-transfection of guide plasmid with psPAX2 and pMD2.G (Addgene, 12260 and 12259, respectively). Conditioned media was harvested ...
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bioRxiv - Biochemistry 2022Quote: ... The HTT expression constructs were assembled using the BD In-Fusion PCR cloning kit in the mammalian/insect cell vector pBMDEL (Addgene plasmid #111751), an unencumbered vector created for open distribution of these reagents.
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bioRxiv - Cell Biology 2022Quote: ... K562 CRISPRi cells with or without MICU1(GFP1-10) were nucleofected with a MTCH2 targeting guide in the pX458 backbone (Addgene plasmid # 48138) using the Lonza SF Cell Line 96-well Nucleofector Kit (V4SC-2096) ...
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bioRxiv - Genomics 2022Quote: Lentiviral particles were produced by transfecting 293T-17 cells (ATCC: CRL-11268) with the envelope construct pCMV-VSV-G (Addgene plasmid 8454), the packaging construct pCMV-dR8.2 dvpr (Addgene plasmid 8455) ...
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bioRxiv - Immunology 2022Quote: ... the cells were transduced with virus produced using the lentiCas9-EGFP plasmid (a gift from Phil Sharp & Feng Zhang, Addgene plasmid # 63592), single-cell sorted using a Sony SH800 cell sorter ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells were generated by infecting cells with lentivirus containing a Cas9-P2A-GFP expression cassette under a doxycycline inducible promoter as described previously (Addgene plasmid #85400)50 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Immunology 2024Quote: ... Production of lentiviral particles was performed by transient co-transfection (polyethylenimine:DNA at 3.5:1 ratio) of HEK 293T cells with the second-generation packaging system plasmid psPAX2 and pMD2.G (Addgene, 12260 and 12259). Viral supernatants were harvested at 48h post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: Lentivirus particles for transduction of IMR90 E6E7 hTERT cells were produced by transfection of an expression plasmid with packaging and envelope plasmids gifted from Didier Trono (Addgene plasmid #12260) and Robert A ...
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bioRxiv - Cancer Biology 2023Quote: ... and SNAI1 knockdown cells were generated by cloning respective shRNA sequences into the 3rd generation lentiviral plasmid pLKO.1 TRC cloning vector (Addgene, cat# 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter ...
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bioRxiv - Biochemistry 2024Quote: ... Lentiviral particles were produced in HEK293 cells after transient transfection of the packaging vectors psPAX.2 and pMD.G2 (Addgene #12260 and #12259). After viral transduction ...
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bioRxiv - Cancer Biology 2024Quote: To generate ER-Hoxa9 mCherry-Geminin cells, the mCherry-Geminin fusion gene (Sakaue-Sawano et al., 2008) was cloned into the pLV-Hygro vector (Addgene plasmid #85134), using Gibson cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected with the viral transfer plasmid lentiCas9-Blast (Sanjana et al., 2014) (gift from Feng Zhang; Addgene plasmid # 52962), the viral packaging plasmid psPAX2 (gift from Didier Trono ...
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bioRxiv - Genetics 2024Quote: ... The Lenti-iCas9-Neo vector containing the Flag-iCas9-P2A-GFP cassette for Cas9 expression in K562 cells was obtained as a gift from Qin Yan (Addgene plasmid #85400,35). For Cas9 expression in HEK293T cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we first replaced the SH3 domain with a flexible linker (GGSSGGGG) using yeast competent cells that were co-transformed with a pCAS plasmid (Addgene plasmid 60847) expressing both the gRNA of interest and Streptococcus pyogenes Cas9 and a donor DNA sequence (stuffer ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus containing all mentioned plasmids was generated by transfecting HEK293T cells with the appropriate plasmid along with the psPAX2 (Addgene; Plasmid #12260) and pMD2.G (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2) or a GFAP-GFP Adeno-associated virus (AAV) construct (Addgene plasmid #50473) for use in animal experiments (utilized in Figure 3 and Figure 4) ...
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bioRxiv - Immunology 2022Quote: ... Virus was generated by transfecting the sgRNA plasmid constructs into HEK 293T cells with the psPAX2 packaging (Didier Trono, Addgene plasmid #12260) and pCMV-VSVG envelope (Bob Weinberg ...
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bioRxiv - Cell Biology 2023Quote: ... pDsRed-RAB11A WT (for expression of a fluorescently-tagged, wild-type version of RAB11A in human cells) was obtained from Addgene (plasmid 12679). For expression of GFP-HEATR5B from baculovirus ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: Murine Taz was expressed by transfecting cells with pEF-TAZ-N-Flag from Michael Yaffe (Addgene #19025; RRID:Addgene_19025; Kanai et al., 2000).
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bioRxiv - Genetics 2023Quote: A phenotypically WT (ROSA+/cre CTIPc/c Bcl2+) v-abl immortalized B cell line was infected with lentivirus for doxycycline-inducible SpCas9 (Addgene Plasmid #50661). Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... HeLa Cells were transduced with the lentiviral expression vector pLenti-H2B-iRFP720 (a gift from Carlos Carmona-Fontaine (Addgene plasmid # 128961; RRID:Addgene_128961) to express fluorescently labeled H2B proteins according to methodology described previously (Segeren et al ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa or HEK293FT cells were co-transfected with the sgRNA plasmid and the pST1374-NLS-flag-linker-Cas9 (Addgene 44758, Xingxu Huang). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, #12259) and pTAT (kind gift of P ...
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bioRxiv - Genetics 2023Quote: ... EPP2 and RN2 cells were retrovirally transduced with shRNAmir constructs cloned into pMSCV-miR-E-PGK-Neo-IRES-mCherry backbone (LENC; Addgene plasmid #111163), and initial infection levels were determined by flow cytometry based on mCherry expression 4 days post transduction (day 0).
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bioRxiv - Genomics 2023Quote: Plasmids in the pCAG backbone used to overexpress TWIST1 and ALX4 in HEK293 cells were cloned by digesting the pCAG-NLS-HA-Bxb1 plasmid (Addgene plasmid # 51271) prepared from dam-/dcm- E ...
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bioRxiv - Biochemistry 2022Quote: ... The following plasmids used for DNA damage experiments in HeLa Tet-On cells were gifts from Kathleen Burns33: pDA007 (Addgene plasmid # 131380), pDA025 (Addgene plasmid # 131384) ...
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bioRxiv - Biochemistry 2023Quote: ... UT Health Sciences Center at San Antonio) and insect cell codon-optimized full-length BARD1 (pFastBac-Twin-StrepTagII-BARD11-777; gift from A. Deans, Addgene plasmid #137166)(Tan et al. ...