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Citations for Addgene :
1501 - 1550 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The respective cell lines were subsequently transduced with the human genome-wide CRISPR-KO (GeCKO, Addgene, #1000000048, #1000000049) sgRNA library at a 1000-fold representation and a multiplicity of infection of <0.3 to ensure one sgRNA integration per cell ...
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bioRxiv - Cell Biology 2021Quote: ... The J8 clonal cell line was infected with lentiCRISPR v2 (a gift from Feng Zhang; Addgene plasmid # 52961), lacking or containing guide RNA against BCAR3 ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa R19 cells stably expressing mCherry-EGFP-LC3 were obtained through stable transfection of plasmid DNA (Addgene #22418), followed by puromycin selection and generation of monoclonal cell populations by limited dilution ...
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bioRxiv - Cell Biology 2021Quote: Measurement of NFAT4 nuclear translocation in HEK293 cells was achieved by transient overexpression of NFAT4-GFP (Addgene #21664) as previously described(30 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells were transfected via PEI (https://www.addgene.org/protocols/transfection/) with pSpCas9(BB)-2A-GFP (PX458) - (Addgene #48138) carrying sgRNA for hSLC9A1 KO 5’-GTTTGCCAACTACGAACACG (SLC9A1:HGLibA_45399 ...
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bioRxiv - Biochemistry 2021Quote: ... Electrocompetent BW25113 cells were co-transformed with the pORTMAGE-4 plasmid (Addgene plasmid #72679, courtesy of Csaba Pál) and the pBAD-yTrm5 plasmid ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells at 70% confluence were transfected with 10 μg of pMRX-IP-GFP-LC3-RFP (#84573, Addgene), 5 μg of VSV.G (#14888 ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2.hSyn-GFP particles were generated by co-transfection of HEK293T cells with AAV2/1 (Addgene 112862), AAV2/2 (Addgene 104963) ...
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bioRxiv - Immunology 2021Quote: ... the RBD expression vectors were co-transfected into Expi293F cells together with the secreted BirA-Flag plasmid (Addgene) in the presence of 100 μM biotin.
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bioRxiv - Genomics 2021Quote: ... These cells were co-transfected with lentiviral packaging plasmids like VSVG (a gift from Bob Weinberg, Addgene #8454) and PAX2 (a gift from Didier Trono ...
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bioRxiv - Cell Biology 2020Quote: ... Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with a viral vector specifically targeting GCaMP6f expression to CaMKII-positive cells (AAV5.CamKII.GCaMP6f.WPRE.SV40, Penn Vector Core via Addgene, CS1024). GCaMP6f injections were targeted at the following coordinates ...
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bioRxiv - Molecular Biology 2020Quote: ... The TRIPZ plasmid was transfected into 293T cells along with lentiviral packaging and envelope 2nd generation plasmids (Addgene packaging 11263 ...
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bioRxiv - Cell Biology 2021Quote: MEF cell lines were transduced with lentivirus containing Cas9 and guide RNA in the lentiCRISPRv2 plasmid (Addgene, 52961) and selected using 2 μg/ml puromycin ...
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bioRxiv - Immunology 2021Quote: ... Cells were co-transfected with either plasmid #166856 or #166857 and a plasmid encoding BirA ligase (Addgene #32408) at a 4:1 ratio (m/m) ...
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bioRxiv - Genetics 2020Quote: ... Cas9 activity was assessed by transducing parental Vero-E6 or Vero-E6-Cas9 cells with pXPR_047 (Addgene 107645), which expresses eGFP and an sgRNA targeting eGFP (72) ...
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bioRxiv - Immunology 2023Quote: ... CHD4 KO cells was similarly transfected with piggy bac plasmid encoding dCas9-KRAB-MeCP2 (45) (Addgene plasmid #110821) and selected with 10 µg/ml blasticidin for 1week then infected with lentiviruses expressing different guide RNAs under U6 promoter.
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/-HEK 293T cells were generated using the CRISPR/Cas9 system from Addgene (http://www.addgene.org/). The sequences for guide RNAs were obtained from http://tools.genome-engineering.org and https://www.addgene.org/pooled-library/zhang-human-gecko-v2/ ...
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bioRxiv - Cell Biology 2023Quote: ... MEF cells were transiently transfected with DRP1 K38A (gift from Alexander van der Bliek & Richard Youle55, Addgene, 45161) using Metafectene Pro (Biontex ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with the plasmid pcDNA3_erGAP2 (a gift from Teresa Alonso and Javier García-Sancho, Addgene #78120), which encodes a low affinity fluorescent calcium biosensor ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses were generated by transfection of 293T cells with the indicated expression plasmid and the psPAX2 (Addgene 12260) and pVSVG (Addgene 14888 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was generated by co-transfection of HEK293 cells with viral vector and packaging plasmids psPAX2 (Addgene 12260) and pMD2.G (Addgene 12259 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated in 6-well plate and co-transfected with SBE4-Luc plasmid (1 μg, Addgene,16495) and pDEST26-Renilla plasmid (0.1 μg ...
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bioRxiv - Microbiology 2023Quote: Lentiviral vector stocks were obtained by transfection of 6.5 x 106 HEK293T cells with packaging vector gag-pol-rev (pCMV-dR8.74, #22036, Addgene), a vesicular stomatitis virus G (VSV-G ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS-Cas9 and U2OSp53KO-Cas9 cells were generated using viral transduction of the lentiCas9-Blast plasmid (Addgene, #52962), followed by a 5-day selection with 5 µg/mL blasticidin ...
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bioRxiv - Molecular Biology 2023Quote: XEN cells were infected with lentiviruses harboring the pHR–SFFV–dCas9–BFP–KRAB vector (Addgene, cat. no. 46911), while ESC v6,5 cells were infected with a modified version of the plasmid in which the SFFV promoter was replaced with an Ef1a promoter 42 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transfected with the mitochondrial calcium indicator pCMV CEPIA2mt (a gift from Masamitsu Iino; Addgene plasmid # 58218 ...
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bioRxiv - Synthetic Biology 2023Quote: Reporter plasmid was co-transformed with accessory and selection plasmids of interest into electrocompetent S1030 cells (Addgene #105063) and recovered using Davis rich media60 (DRM) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Genomics 2023Quote: AAV production was performed by transfecting 293FT cells with 22 ug of pDGM6 helper plasmid (Addgene plasmid # 110660), 6 ug of donor template plasmid ...
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bioRxiv - Genetics 2023Quote: ... 293T cells were transfected with 7.5 μg of of the plasmid pCMVR8.74 (a gift from Didier Trono (Addgene plasmid #22036 ...
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bioRxiv - Neuroscience 2022Quote: ... Lentivirus was created from this plasmid by co-transfecting HEK293T cells with this plasmid alongside psPAX2 (Addgene #12260) and MD2.G (Addgene #12259) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T WT cells were transfected using Lipofectamine3000 with pLenti plasmids and lentiviral packaging plasmids (Gag-pol (Addgene #14887), VSV-G (Addgene #8454) ...
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bioRxiv - Neuroscience 2022Quote: ... Lipofectamine3000 was used to transfect cells with 0.5ug of pSpCas9(BB)-2A-GFP(PX458) targeting plasmid (Addgene # 48138) containing sgRNA sequences targeting full-length or truncations in SRRM2 and PNN (Supplemental Table 3) ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthetic cell type specific promoters were obtained from the following Addgene plasmids: 161_pAAV-ProD5-CatCh-GFP-WPRE (Addgene plasmid # 125981 ...
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bioRxiv - Genomics 2022Quote: Twenty million cell batches of J1 ESCs were transfected each with 20 μg dCas9-VPR vector (Addgene #63798) and 20 μg plasmid constructed by the IGBMC molecular biology platform (available on request) ...
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bioRxiv - Molecular Biology 2024Quote: ... the HEK293T cells were transfected with the respective constructs along with envelope plasmid (pMD2.G) (Addgene, Cat# 12259), and packaging plasmid (psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: ... AICSDP-42:AAVS1-mTagRFPT-CAAX was a gift from Allen Institute for Cell Science (Addgene plasmid # 107580; http://n2t.net/addgene:107580; RRID:Addgene_107580). APEX2-NLS ...
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bioRxiv - Cell Biology 2024Quote: ... AICSDP-82:SON-mEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid # 133964; http://n2t.net/addgene:133964; RRID:Addgene_133964). AICSDP-42:AAVS1-mTagRFPT-CAAX was a gift from Allen Institute for Cell Science (Addgene plasmid # 107580 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were co-transfected with pcDNA3.0 plasmids containing human CDC50A (NM_018247, N-terminal FLAG tag; RRID: Addgene_203694) and ATP10B variants (O94823.2 ...
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bioRxiv - Cancer Biology 2023Quote: Retroviral supernatants were prepared by co-transfecting 293T cells (DSMZ, cat ACC635) using pCL ECO (Addgene, cat 12371) for mouse cells or pCL ampho for human cells and a retroviral vector ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transfected with either (G4C2)92 and (G4C2)2 lentiviral transfer plasmids along with PAX (Addgene #12260) and VSV-G (Addgene #12259 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK-293 cells were transfected with two plasmid vectors CMV–R-GECO1 (a gift from Robert Campbell, Addgene plasmid #32444 ...
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bioRxiv - Molecular Biology 2024Quote: ... MutuI cells were transduced with lentiparticles derived from the doxycycline inducible Cas9 vector pCW-Cas9 (Addgene Plasmid #50661) and selected for stable integration with 2 μg/mL puromycin ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were reprogrammed by a mixture of retroviral vectors encoding OCT3/4 (pMXs-hOCT3/4 Addgene: 17217), SOX2 (pMXs-hSOX2 Addgene ...
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bioRxiv - Cell Biology 2024Quote: B16-F1 cells were transiently transfected with CYRI-B-p17-GFP and mCherry-β1 integrin (Addgene plasmid #55064) and plated on laminin coated glass bottom dishes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088 ...
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bioRxiv - Cell Biology 2023Quote: ... reporter cells were transduced with pMH0007-UCOE-pEF1ɑ-Cas9-HA-2xNLS-BFP (Addgene #174162, (Hein and Weissman, 2021)) ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviral particles were harvested from supernatants of HEK293T cells that had been co-transfected with psPAX2 (Addgene 12260), pMD2.G (Addgene 12259) ...
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bioRxiv - Cell Biology 2023Quote: ... in HEK 293T cells (ATCC, VA) using 3rd generation lentiviral packaging plasmids pMDLg/pRRE (Addgene Inc., Plasmid 12251) and pRSV-RV (Addgene Inc. ...