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Citations for Addgene :
1351 - 1400 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... A375-dCas or HeLa-dCas cells were generated by transducing with pLenti-dCas9-VP64-Blast (Addgene, #61425). After 7 days of blasticidin selection ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by transformation into competent cells according to an online protocol (GeCKO library Amplification Protocol from Addgene). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Cancer Biology 2021Quote: ... and pCMV-VSV-G/pCMV-dR8.2 for human cell lines (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454 and Addgene plasmid #8455 ...
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bioRxiv - Cell Biology 2021Quote: ... NCI-H1573 and NCI-H1299 cells were transfected with LeGO lentiviral particles (Addgene plasmids #27338, #27339, #27340) (Weber et al. ...
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bioRxiv - Cancer Biology 2022Quote: T98GIRF3-/- and T98GCTRL cell lines were generated using the LentiCRISPRv2GFP plasmid (gift from David Feldser; Addgene # 82416). IRF3 or control guide RNAs were inserted into the LentiCRISPRv2GFP plasmid following provided guidelines and lentiviral particles generated ...
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bioRxiv - Molecular Biology 2022Quote: ... 2014) was co-transfected into 293FT cells with the lentiviral packaging plasmids psPAX2 and pMD2.G (Addgene). Viral production was accomplished as described previously (Joung et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transduced with floxed jGCaMP7b (AAV1-syn-FLEX-jGCaMP7b-WPRE; Addgene #104493, MOI = 5×105 vg) [14] and low-titer Cre (AAV9-hSyn-Cre-WPRE-hGH ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus stocks were produced in Hek293T cells using pCMVΔR8.91 as packaging plasmid and pMD2.G (Addgene #12259) as vesicular stomatitis virus G glycoprotein (VSV-G ...
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bioRxiv - Microbiology 2022Quote: ... Early passage HEK293T cells were transfected with 1.5 μg of dCas9-KRAB-MeCP2 repressor plasmid (Addgene #110824) and 0.5 μg of Piggyback Transposase (gift from Maxim Greenberg) ...
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bioRxiv - Microbiology 2022Quote: Hela-H1 cells were transfected with a plasmid encoding the VSV-G gene (pMD2.G; Addgene #12259) using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260) and pMD2.G (Addgene 12259) ...
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bioRxiv - Biophysics 2022Quote: ... cells constitutively expressing CheRiff were transiently transfected with pCAG-Kir2.1-T2A-tdTomato (from Massimo Scanziani, Addgene 60598) using CalFectin transfection reagent (SignaGen Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... These cells (1.5 × 106) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer (Le et al. ...
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bioRxiv - Neuroscience 2020Quote: ... for imaging hippocampal pyramidal cells or pAAV-mDlx-GCaMP6f-Fishell-2 (a gift from Gordon Fishell, Addgene plasmid # 83899 ...
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bioRxiv - Molecular Biology 2020Quote: ... For generation of the RPE-1-H2B-mEGFP-FKBP stable cell line the H2B-mEGFP (Addgene #105528) and FKBP (Promega N201A ...
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bioRxiv - Neuroscience 2021Quote: ... 3 × 106 HEK293T cells were transfected using GenJet transfection reagent (Signagen) with 7.5 µg psPax2 (Addgene #12260), 5 µg VSV-G (pMD2.G ...
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bioRxiv - Neuroscience 2022Quote: ... Mammalian cells were transfected with either the empty vector (pAAV) or human WT aSyn pAAV vector (Addgene plasmid # 36055 ...
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bioRxiv - Developmental Biology 2022Quote: NPC-iPS cells (clone 16-13(Maetzel et al., 2014)) were targeted with AAVS1-tdTomato (Addgene 159275) as described before(Ma et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... the transfer plasmid was co-transfected into HEK-293T cells with the packaging plasmids pVSVg (Addgene, #8454) and psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR screen was performed in BT20 cells using the GeCKO v2 two vector system (Addgene, CAT# 1000000049). Both A and B libraries were amplified according to the distributor ...
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bioRxiv - Molecular Biology 2020Quote: ... These cells were spin transfected for 2h at 1000g with 82*10E6 Brunello virus particles (LentiCRISPRv2, Addgene 73179-LV ...
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bioRxiv - Microbiology 2020Quote: ... Lentiviral particles were generated by co-transfection of 293T cells with pLenti6-Dest_neo_ACE2-2A-Bla or pLenti6-Dest_Puro_TMPRSS2 together with pCMVR8.74 (Addgene plasmid 22036) and pMD2.G (Addgene plasmid 12259 ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were reverse-transfected with 3 plasmids: psPAX2 (a gift from Didier Trono, Addgene #12260), pEGFP-Vpr (obtained through the NIH HIV Reagent Program ...
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bioRxiv - Immunology 2021Quote: Generation of Scid.adh.2c2-dCas910x-GCN4 CRISPRi cells dCas910x-GCN4 (pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP, Addgene #60904) was lentivirally transduced into Scid.adh.2c2 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Lenti- sgRNA/Cre vectors were individually co-transfected into 293T cells with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 U2OS cells were transiently co-transfected with 200 ng gRNA expression plasmid (cloned into Addgene 43860), 500 ng Cas9 expression plasmid (Addgene 43945) ...
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bioRxiv - Cancer Biology 2021Quote: ... H4 human GBM cells were infected with the whole-genome knockout Brunello library (Addgene, Cambridge, MA, USA), which covered ~19,000 genes with 4 sgRNAs per gene along with 10,000 sgRNA non-targeting controls ...
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bioRxiv - Microbiology 2020Quote: ... lentiviruses were produced by co-transfecting HEK293T cells with a plasmid encoding VSV-G (Addgene cat#12259), a lentiviral Gag-Pol packaging plasmid (Addgene cat#8455) ...
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bioRxiv - Molecular Biology 2021Quote: ... RPE1-hTERT cells were co-transfected with either of these vectors and the hCas9 vector (Addgene #41815) using a Neon Transfection system (Thermo ...
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bioRxiv - Molecular Biology 2021Quote: ... The RPE1-hTERT TP53-/- RNF8-/- Cas9 cell line was generated by electroporation of phU6-gRNA (Addgene #53188) plasmids expressing sgRNAs (5’-CCCAGAGTCTAAATGGTGTT-3’ and 5’-GGAAGAGGAACAGCATCTTC-3’) ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus packaging was performed by using MaxPEI-based co-transfection of HEK293T cells with psPAX2 (Addgene #12260), pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected using Lipofectamine 2000 (Thermo) with the pSpCas9(BB)-2A-puro (px459) V2.0 plasmid (Addgene) containing CRISPR guide-RNAs (gRNAs ...
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bioRxiv - Microbiology 2023Quote: ... along with a luciferase gene with SARS-CoV-2 packaging sequence PS9 into 293T cells (Addgene 177942). Plasmids were gifts from Jennifer Doudna ...
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bioRxiv - Developmental Biology 2023Quote: ... TCMC-OGFP cell line was generated by nucleofecting 1µg supercoiled Oct4-IRES-eGFP-PGK-Neo (Addgene #48681) plasmid to 1 million TCMC using P3 primary cell 4D-Nucelofector X kit (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human melanoma cell lines with fluorescent BRN2 reporter were further transduced with pHIV-Luc-ZsGreen (Addgene 39196) and selected with neomycin (800 μg/mL ...
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bioRxiv - Genomics 2023Quote: ... K562 CRISPRi and RPE1 CRISPRi cells expressing dCas9-BFP-KRAB (pHR-SFFV-dCas9-BFP-KRAB; Addgene, 46911) were described previously.136 Lenti-X 293T cells were purchased from Takara (#632180) ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells expressing DDX21-HA or OMP25-eGFP (expressed using pMXs-3XHA-EGFP-OMP25, Addgene: plasmid #83356) were cultured in 1x DMEM based media (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293FT cells were co-transfected with the Tet-on ssRFP-GFP-KDEL plasmid (Addgene 128257, Noboru Mizushima) and the lentivirus packaging plasmids psPAX2 (Addgene 12260 ...
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bioRxiv - Molecular Biology 2023Quote: Cells were plated in 10-cm plates and transfected with plasmids expressing His6-SUMO1 (Addgene plasmid # 133770) or His6-SUMO2 (Addgene plasmid # 133771) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were co-transfected with 250ng of 3X ERE with TATA-box luciferase plasmid (Addgene 11354), a gift from Donald McDonnell ...
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bioRxiv - Genomics 2023Quote: ... Control cells were co-transfected with pcDNA3.1-IRES-GFP28 (Addgene plasmid # 51406; http://n2t.net/addgene:51406; RRID:Addgene_51406) and the mCherry reporter construct above ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles for transduction were generated from 293T cells transfected with psPAX2 (Didier Trono, Addgene plasmid # 12260) and pMD.2 (Didier Trono ...
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bioRxiv - Cell Biology 2023Quote: Calu-1ERN1-/-cells were constructed by transfection with pSpCas9n(BB)-2A-Puro (Feng Zhang lab, Addgene #48139) containing sequences for guides targeting the start codon (WGE ID’s 1150311103 and 1150311093) ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was generated in HEK293T cells by transfection with the aforementioned transfer vectors and pMDLg (Addgene 12251), pRSV-REV (Addgene 12253) ...
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bioRxiv - Molecular Biology 2022Quote: ... and cells were transfected with 500 ng of CAG-Cas9-T2A-EGFP-ires-puro-plasmid (Addgene, #78311) and with 250 ng of gRNAs altogether using PEI transfection reagent with addition of 0.15 M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: HEK-293T cell line was transduced with a Cas9 lentiviral vector with blasticidin selection marker (Addgene# 52962) and selected with blasticidin (8 ug/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... L363 and KMS12 cells were transduced with the lentiviral vector pInducer20 (RRID:Addgene 44012, Gift of Stephen Elledge) harboring a Tet operator-controlled KDM6A cDNA (pKDM6A ...
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bioRxiv - Cancer Biology 2024Quote: ... MEF cells were transduced with the retroviral vector pBABE-puro-KRASG12V (Gift from Christopher Counter (RRID:Addgene 46746) or a control plasmid pBABE-puro (Gift from Hartmut Land ...
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bioRxiv - Neuroscience 2024Quote: 120 000 iPS cells were plated on 12-well plates one day before the transduction by lentiviruses pLV_TRET_hNgn2_UBC_Puro (Addgene: 61474) and pLV_hEF1a-rtTA3 (Addgene:61472 ...