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Citations for Addgene :
1401 - 1450 of 2264 citations for Mouse EGF Like Repeat And Discoidin I Like Domain Containing Protein 3 EDIL3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... in a lentiviral backbone containing a puromycin resistance sequence (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro, a gift from Charles Gersbach, Addgene plasmid # 71236 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 x 108 Huh7 cells for each of two replicates were transduced at a MOI of ~0.3 with lentivirus containing the Brunello genome-wide library in lentiCRISPRv2 (Addgene 73179), which contains 77.441 sgRNAs targeting 19.114 genes ...
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bioRxiv - Cell Biology 2021Quote: ... Oligos were ordered and BsmBI batch-cloned into the gRNA expression core of a LentiCRISPR_v2 plasmid containing Cas9 (Addgene 52961). The library was amplified in Stbl3 (Thermo ...
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bioRxiv - Genomics 2021Quote: Toronto human knockout pooled library (TKOv3) containing 71,090 based on a lentiCRISPRv2 backbone was a gift from Jason Moffat (Addgene #90294). The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen ...
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bioRxiv - Molecular Biology 2021Quote: ... 180 bp upstream of the stop codon and 500 bp after the FOXO1 top codon were cloned into pUC19 containing FKBP12F36V-2xHA-P2A-mCherry derived from Addgene #104370 (pAW6214 ...
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bioRxiv - Cancer Biology 2022Quote: ... a central region containing a triple FLAG epitope and P2A-neomycin resistance cassette acquired via restriction endonuclease digestion of pFETCH_Donor (Addgene: 63934); and 3 ...
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bioRxiv - Immunology 2022Quote: ... 3×106 cells were harvested and added to 100 µl supplemented Nucleofector Solution V containing 0.7 µg CIBN-CAAX (Addgene #79574), 1.1 µg CRY2PHRmcherry2-RASAL3 ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana plants constitutively expressing Cas9 (Cas9 Benthe 193.22 T5 Homozygous; gratefully received from Dan Voytas) together with a strain containing pTRV1 (Addgene #148968) as previously described (Ellison et al. ...
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bioRxiv - Neuroscience 2024Quote: ... A pulled glass pipette tip of 20–30 μm containing CTB647 (ThermoFischer Scientific, C34778) or retrograde AAV (Addgene, AAV-PHP.eB) and FG was lowered into the brain ...
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bioRxiv - Neuroscience 2024Quote: ... and a micropipette containing 100nL of pAAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (Addgene 71760-AAV1; titer: 9.8×1012vg/mL) was injected unilaterally into the VTA of DAT-IRES-Cre mice.
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bioRxiv - Cell Biology 2023Quote: ... to co- transfect HEK 293FT cells with plasmids containing the packaging gag/pol and envelope pCMV- 435VSV-G genes and either the pMRX-IB-HaloTag7-mGFP-KDEL (Addgene, 184904 ...
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bioRxiv - Molecular Biology 2023Quote: ... Jump-in TurboID cell lines were prepared by co-transfecting HEK293T cells with 1.0 μg and 1.5 μg of attb-BSDr containing the gene of interest and pCMV-Int (ΦC31-integrase, Addgene 18935) plasmids respectively ...
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bioRxiv - Cell Biology 2023Quote: ... All intermediary plasmids containing respective sgRNA were then cloned into the final pLV hUbC-Cas9-T2A-GFP plasmid (Addgene #53190), where GFP was replaced by puromycin resistance.
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bioRxiv - Bioengineering 2023Quote: ... and a plasmid containing both an empty sgRNA scaffold as well as a gene sequence encoding SpCas9 (AddGene plasmid# 57819) with a final goal of LV positive control vector generation ...
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bioRxiv - Immunology 2023Quote: ... The lentiCRISPRv2-puro vector containing the Cas9 expression cassette and the gRNA scaffold was a gift from Brett Stringer (Addgene plasmid #98290 ...
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bioRxiv - Biochemistry 2023Quote: ... human EZH2 wildtype and the K20R or S21A mutant of human EZH2 were cloned into the retroviral pMSCV-Puro vector containing 3xFlag-3xHA epitope (Addgene) and the recombinant retroviruses were packaged in 293 cells (Guo et al. ...
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bioRxiv - Bioengineering 2023Quote: The pSECRETS-C plasmids containing desired on-target sequences were constructed via HiFi assembly into the p11.LacY.wtx1 plasmid (Addgene #69056), which was double digested with XbaI and SphI ...
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bioRxiv - Neuroscience 2023Quote: ... A virus lacking the hM4Di DREADD gene and instead containing the fluorescent tag eGFP (AAV8-CaMKIIa-EGFP, packaged by Addgene) was infused into ACC in 4 animals as a null virus control ...
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bioRxiv - Cell Biology 2023Quote: The human sFLT1-HA plasmid construct created through Gibson cloning of human sFLT1 cDNA into a pcDNA3.1 vector containing a C-terminal HA tag (pcDNA3-ALK2-HA, Addgene 80870). Sequencing validation was performed through Genewiz ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-line(#4954) was transduced using viral particles containing the lentiCas9-Blast vector (Addgene, Watertown, MA; RRID:Addgene_52962). Cells stably expressing Cas9 were selected using 10ug/ml Blasticidin S HCl (Gibco™-#R21001) ...
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bioRxiv - Biophysics 2023Quote: ... 3C protease site cloned into a pcDNA3 vector containing human IgG Fc derived from pcDNA3-Nrxn1beta AS4(-)-Fc (a gift from Peter Scheiffele & Tito Serafini, Addgene plasmid # 59313 ...
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bioRxiv - Microbiology 2023Quote: ... A fragment of MeCP2 containing the T158M mutation was amplified by PCR and cloned in pDEST-hMeCP2-GFP (a gift from Huda Zoghbi, Addgene plasmid # 48078 ...
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bioRxiv - Biochemistry 2023Quote: Mass spectrometry sample preparation: N2A cells were transfected with pcDNA3 plasmids containing 2X myc-EXOSC3 or pcDNA3 empty vector (Addgene) using Lipofectamine® 2000 (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... A 250 µL aliquot of lentiviral cocktail containing either Lenti-Cas9-Blast plasmid (SU-DIPG-XIII; Addgene, Watertown, MA, USA) or Lenti-Cas9-2A-Blast (SU-DIPG-XXXVI ...
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bioRxiv - Neuroscience 2023Quote: ... slices were virally infected with 0.5-1 µl AAV mixture per slice (containing AAV9-Camk2a-Cre at 2 × 1012 vg/ml (1:1000 dilution, Addgene and rAAV8-DIO-CBA-pAAIP2-mEGP at 4.2 x 1012 vg/ml ...
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bioRxiv - Biochemistry 2023Quote: A pET29a-YS14 plasmid containing Xenopus laevis histones was obtained as a gift from Jung-Hyun Min (Addgene plasmid # 66890). H2A-H2B was subcloned into pET His6 TEV LIC cloning vector (2B-T ...
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bioRxiv - Biochemistry 2023Quote: Phosphorylated tauC3 was expressed in Sf9 insect cells from a 438B pFastBac vector containing an engineered 6xHis tag and a TEV protease cleavage site (Addgene). Following two rounds of virus amplification ...
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bioRxiv - Cell Biology 2023Quote: Entry clones were transferred using Gateway technology into a mammalian expression pcDNA3.1 plasmid containing an N-terminal 3xFLAG-V5 tag (Addgene 87064) for the Target Accelerator Pan-Cancer Mutant Collection ...
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bioRxiv - Synthetic Biology 2023Quote: ... construct was made by also acquiring a lentiviral transfer plasmid containing the GAL4-VP64 inducible upstream activator sequence (UAS) from Addgene (pHR_5x Gal4 UAS was a gift from Wendell Lim (Addgene plasmid # 79119 ...
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bioRxiv - Cell Biology 2023Quote: ... The pCAG-V5-TurboID vector was constructed by inserting the amplified TurboID cDNA containing the V5 epitope (GKPIPNPLLGLDST) sequence at the N-and C-terminal sites into the pCAGGS expression plasmid (Addgene). Expression plasmids encoding V5-TurboID-Solo and Solo-TurboID-V5 were constructed by inserting amplified Solo cDNA with a GS-linker (GGGSx2 ...
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bioRxiv - Neuroscience 2023Quote: ... UBA5) were generated as described above with the following modifications: third-generation packaging DNA mix containing pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Neuroscience 2023Quote: ... the plasmid to be packaged was co-transfected into HEK293T cells with a rep/cap containing plasmid pUCmini-iCAP-PHP.eB (Addgene #103005) and the helper plasmid pAdDeltaF6 (Addgene #112867) ...
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bioRxiv - Cancer Biology 2024Quote: ... A sequence containing the 2.4-kbp p21 promoter region was PCR amplified from the pGL2-p21 promoter- Luc (Addgene #33021). The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a- mCherry-3X-NLS (Addgene #125262) ...
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bioRxiv - Cancer Biology 2024Quote: Truncated CPSF6 containing amino acids 1-358 (CPSF6-358) from human isoform 1 was cloned into the pCW57.1 backbone (Addgene 71782). A hemagglutinin (HA ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into the lentiviral CRISPR-Cas9 delivery vector containing spCas9-eGFP under control of the EFS promoter and the sgRNA under control of the U6 promoter (Addgene). Following transfection into competent Stbl3 bacteria (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... we amplified a TetR expression cassette containing the cytomegalovirus promoter (P.CMV) and the coding sequence of TetR from pDM291-tetR-tup11Δ70 (Addgene plasmid 41027) (Zilio et al. ...
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bioRxiv - Neuroscience 2024Quote: All cDNAs for human wild-type and mutant forms of CHCHD10 were synthesized and inserted in the pcDNA3 vector containing FLAG-tag by Genescripts and mCherryLC3B (#40827) plasmid was obtained from Addgene.
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bioRxiv - Molecular Biology 2024Quote: ... we amplified a fragment of the Cas9 gene containing the H840A mutation from the pRS415_pGal-nCas9(H840A) plasmid (Addgene #79616) with primers NcoI_Cas9_F and Cas9_R ...
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bioRxiv - Cancer Biology 2021Quote: ... HT-1080N cells were transduced with lentiviruses carrying the reverse tetracycline-controlled transactivator 3 under a CMV promoter (CMV-rtTA3) (Cat# w756-1, Addgene). HT-1080N-rtTA3 cell lines were selected in 10 μg/mL blasticidin (Cat # A1113902 ...
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bioRxiv - Cell Biology 2020Quote: ... designed by Life technologies to target the second exon of murine Vangl2 (gRNA: 5’-TCGGCTATTCCTACAAGTC-3’) into pSpCas9(BB)-2A-GFP (PX458, Addgene #48138). Upon transfection ...
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Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor nichebioRxiv - Developmental Biology 2022Quote: ... The T2A-nls:Gal4:VP16-STOP sequence was generated by PCR (Supplementary Table 3) from the pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB vector (Addgene). 5’HA-T2A-nls:Gal4:VP16-STOP-3’HA was assembled in correct 5’-to-3’ order between Not1 and EcoR1 sites of the pJet1.2 vector.
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bioRxiv - Microbiology 2022Quote: ... All shRNAs were generated by cloning shRNA hairpin sequences found in Table 3 into pLKO.1-TRC Puro (pLKO.1-TRC cloning vector was a gift from David Root (Addgene plasmid # 10878 ...
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bioRxiv - Neuroscience 2021Quote: ... The two fragments (5’ homology arm and 3’ homology arm) were assembled into plasmid pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5 (Addgene) that was linearized with XbaI and HindIII using In Fusion cloning ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-GGG GGG GGC GGA ATT CTC AGT CTA TCT TTT CTT TCT GGA CCA GTC TGG GAG C-3’ and pmScarlet-C1 (gift from Dorus Gadella; Addgene plasmid # 85042 ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS 2-6-3 were transduced with pLenti CMV rtTA3 Blast (w756-1, plasmid #26429; Addgene, gift from E. Campeau) for the expression of rtTA ...
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bioRxiv - Genomics 2020Quote: ... and a revers primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-GTGTCTCAAGATCTAGTTACGCCAAGC-3′) were used to amplify 222 bp fragments from CROPseq-Guide-Puro plasmids (#86708, Addgene) which have been inserted with different gRNA sequences ...
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bioRxiv - Molecular Biology 2019Quote: ... sgRNAs that target the 3′ end of the respective coding sequences were cloned into hSpCas9 plasmid (PX458, Addgene Plasmid #48138) (Ran et al ...
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bioRxiv - Molecular Biology 2019Quote: cDNA from Source BioSicence clone C130076G01 was amplified with PCR adding restriction sites for NheI at 5’ end and AgeI at 3’ end (Supplementary Table 2) and cloned into pLIX_402 vector (a gift from David Root, Addgene #41394) using restriction ligation ...
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bioRxiv - Molecular Biology 2019Quote: ... 2×106 mEF-depleted cells were transfected with sgRNAs 2+3 and pCas9_GFP (a gift from Kiran Musunuru, Addgene #44719). To generate Chaserrb/b mESCs ...
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bioRxiv - Molecular Biology 2021Quote: ... or Ring1b (5’-ACAAAGAGTGTCCTACCTGT -3’) were introduced into a modified version of the vector plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230) that yields a BFP marker for selection (Gift by J ...