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Citations for Addgene :
1551 - 1600 of 2264 citations for Mouse EGF Like Repeat And Discoidin I Like Domain Containing Protein 3 EDIL3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... the protein coding sequence (CDS) of RLuc8.6 was amplified from pcDNA-RLuc8.6-53559 (Addgene ID 87125) and subcloned into the vector pRSETb115 (Addgene ID 89536 ...
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bioRxiv - Plant Biology 2019Quote: ... DNA-oligonucleotides (Figure 2-source data 1) containing the specific gRNA sequence were synthesised and used to amplify the full gRNA from a template plasmid (AddGene #46966). Using Golden Gate cloning40 each gRNA was then recombined in a L1 vector downstream of U6 promoter39 ...
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bioRxiv - Cell Biology 2020Quote: ... obtained from Dharmacon) into pUAST vectors containing a C-terminal Venus open reading frame (Wang et. al, 2012, Addgene plasmid 35204). Transgenes were sequenced and then injected into embryos containing an attP8 site on the X chromosome (BDSC stock #32233 ...
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bioRxiv - Microbiology 2020Quote: ... A DNA fragment containing a codon-optimized version of hACE2 (Genbank NM_021804) was inserted into pLV-EF1a-IRES-Hygro (Addgene Plasmid #85134) using Gibson assembly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding phiC31 integrase under control of a heat shock promoter (Addgene #26290) [73]) ...
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bioRxiv - Microbiology 2022Quote: ... were introduced into a previously described spike-expression plasmid containing D614G and a 21-amino-acid deletion in the cytoplasmic tail (Addgene 158762) (34) ...
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bioRxiv - Neuroscience 2022Quote: ... 90ul cells suspension containing 1M cells was mixed with 10 uL DNA mix: 4 ug pSpCas9(BB)-2A-Puro (PX459) plasmid (Addgene #48139), • 0.4 ug gRNA encoding plasmid (pKLV-U6gRNA(BbsI)-PGKzeo2ABFP ...
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bioRxiv - Immunology 2019Quote: We cloned template DNA for the indicated guide RNAs into a pLX-sgRNA construct additionally containing blasticidin resistance (Addgene plasmid #50662). We transfected constructs into HEK293T cells along with lentivirus packaging vector pSPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Molecular Biology 2019Quote: ... N-terminal 3xFLAG_V5_GFP-tagging of endogenous nxf2 and panoramix loci was done by co-injecting a repair template and the gRNA containing plasmid (Addgene 45956) into act-Cas9 flies (BL-58492) ...
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bioRxiv - Synthetic Biology 2019Quote: Plasmids containing different TEV protease versions were episomally introduced in a pRSII413 vector which was a gift from Steven Haase (Addgene #35450). Transformed cells containing the HIS3 gene were selected on SMM plates (SMM with 20 g/L agar ...
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bioRxiv - Molecular Biology 2019Quote: ... Retroviral vectors for JMJD6 expression were generated by cloning the Jmjd6 coding sequence with a C-terminal FLAG-tag sequence (11) into the pBABE plasmid vector containing the neomycin resistance gene (Addgene #1767). Mutant versions of JMJD6 were generated from the wild type construct using QuikChange Lightning site-directed mutagenesis kit (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... The pCMV(CAT)T7-SB100 containing the CMV promoter and SB100X transposase was a gift to Addgene from Zsuzsanna Izsvak (Addgene # 34879) 78 ...
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bioRxiv - Developmental Biology 2019Quote: ... using AscI and NotI-containing primers and cloned the fragment into the vector p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] (Addgene plasmid #71809). Transgenic lines were generated by Genetivision Inc ...
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bioRxiv - Cell Biology 2019Quote: ... The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397) as a gift from David Liu [31] ...
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bioRxiv - Developmental Biology 2019Quote: ... p118 PB-TRE-dCas9-VPR 70 containing dCas9 fused to VP64-p65-RTA (dCAS9-VPR) transcriptional activators and hygromycin resistance gene (Addgene, #63800) and a plasmid coding for the PiggyBac transposase (kind gift from Valentina Carlini) ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... 1 pmol of the oligo mix was added to a 20 μl Golden Gate reaction containing 100 ng destination plasmid (pATT-DEST, Addgene #79770), 10 units BsaI (NEB #R3733) ...
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bioRxiv - Cancer Biology 2020Quote: ... a sox10 minimal promoter was PCR amplified from genomic DNA from AB* larvae with primers (Table 1 and 2) containing overlapping nucleotides to sequences on either side of the AgeI site in pENTR-EGFP2 (Addgene #22450), similarly described in (Quillien et al. ...
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bioRxiv - Microbiology 2020Quote: RAW 264.7 cells stably expressing FL-Cas9 were generated by transducing RAW 264.7 cells with lentivirus containing LentiCas9-Blast (Addgene plasmid #52962)(Sanjana et al. ...
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bioRxiv - Systems Biology 2021Quote: All sgRNAs were synthesized as individual oligo pairs (IDT) and cloned into a barcode library containing plasmid pool (pBA571, Addgene #85968), thereby linking each sgRNA to a unique guide barcode contained within the 3′-UTR of the puromycin resistance gene (33) ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were transfected with a mix of 8 μg lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library 21 (Addgene #90294), 4.8 μg packaging vector psPAX2 ...
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bioRxiv - Neuroscience 2020Quote: ... with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Full-length human KRAS4B G12C was ectopically expressed from the retroviral expression vector pBABE containing an N-terminal HA-tag (Addgene #58901). Viral particles were generated by transient transfection of each expression vector into HEK 293T cells using Fugene6 (Promega ...
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bioRxiv - Microbiology 2022Quote: HK1 cells that stably express GFP-LMP1 under control of a tetracycline-inducible promoter were created by first being transduced with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; 17492) as previously described in (18) ...
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bioRxiv - Biophysics 2022Quote: ... a pQE-30/pcl-ts ind+ plasmid containing a His6-SUMO SARS-CoV-2 nsp12 and untagged nsp7 & 8 (Addgene #160540) was transformed into E ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9-expressing A375 cells were transduced with lentivirus containing expression plasmid pXPR-011 (gift of John Doench and David Root; Addgene #59702), which encodes for enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cancer Biology 2022Quote: ... T98G Sin3B+/+ and Sin3B-/- TLR cell lines were created and then infected with lentivirus containing SceI and the template for eGFP reconstitution (Addgene #32627), after 72 hours ...
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bioRxiv - Immunology 2022Quote: ... Vector psPAX2 containing the untagged coding sequence for HIV-1 gag-pol was a gift from Didier Trono (Addgene plasmid # 12260).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... approximately 500,000 cells in 6-well plates were transfected with 2000ng dCas9-KRAB-MeCP2-containing PiggyBac expression plasmids (Addgene plasmid #110821) and 400ng of transposase vector PB200PA-1 using PEI ...
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bioRxiv - Genetics 2021Quote: ... followed by further introduction of either the plasmid containing sgRNA targeted to rs117132860 or a non-targeting sgRNA (Addgene, Cat#80226). Following selection ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR product containing CHD4 was cloned into a modified pFastBac vector (a gift from S. Gradia, UC Berkeley, vector 438-C, Addgene: 55220) via LIC ...
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bioRxiv - Developmental Biology 2019Quote: Four gRNAs targeting loci on both sides of the C-terminal region of Rok containing the putative phosphorylation sites to be mutated were cloned into pCFD3 vector (Addgene 49410) following the protocol from (Port et al. ...
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bioRxiv - Genetics 2020Quote: ... were transduced at a low multiplicity of infection (MOI) with virus containing sgRNA library cloned in lentiGuide-Puro (Addgene plasmid 52963) to ensure that most cells received only one sgRNA in expansion phase medium42 ...
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bioRxiv - Cell Biology 2021Quote: ... A gBlock containing sequence for SNAP-V5 tags was inserted into pLenti CMVTRE3G eGFP Blast (w818-1) (gift of Eric Campenau, Addgene #27568) at the AgeI restriction site using Gibson Assembly to make pLTRE3G-SNAP-V5-eGFP ...
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bioRxiv - Cancer Biology 2021Quote: ... were amplified using primers containing overhangs with the homology sites for GMAP cloning and inserted into a lentiviral vector (LV 1-5, Addgene, 68411). This lentiviral backbone was a gift from Dr ...
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bioRxiv - Genomics 2020Quote: ... plasmids containing sequences for dCas9 and CTCF were generated by modifying lenti-dCas-VP64-Blast (a gift from Feng Zhang, Addgene #61425). The VP64 cassette was replaced by CTCF sequences to generate dCas9-CTCF and neomycin resistant marker that was taken from pAC95-pmax-dCas9VP160-2A-neo (a gift from Rudolf Jaenisch ...
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bioRxiv - Genetics 2020Quote: ... the Cbr-unc-119 rescue gene and a portion of the homology arms containing the guide site were removed from pCFJ151(Addgene #19330) (Frøkjær-Jensen et al ...
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bioRxiv - Cell Biology 2022Quote: ... The N-terminal GFP-tagged Tlg2p was expressed as follows: the SacI and HindIII fragment (containing iGFP-TLG2) extracted from YLplac211-iGFP-TLG2 plasmid (Addgene #105262) was inserted into the SacI and EcoRV-digested pRS303 (pRS303-iGFP-Tlg2) ...
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bioRxiv - Developmental Biology 2022Quote: ... were cloned into a Cas9-T2A-Puromycin expressing plasmid containing the U6-gRNA scaffold (gift of Andrea Németh, Addgene plasmid #101039). mES cells were transfected with 8 µg of plasmid using the Promega FuGENE 6 transfection kit and dissociated 48h later for puromycin selection (1.5 µg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... were amplified using primers containing overhangs with the homology sites for GMAP cloning and inserted into a lentiviral vector (LV 1-5; Addgene, 68411). IL6-EGFP from pmIL-6promoterEGFP (Addgene 112896) ...
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bioRxiv - Systems Biology 2023Quote: ... We ordered pairs of complementary forward and reverse single stranded oligonucleotides (IDT) for each guide containing compatible overhang sites for insertion into a lentiCRISPRv2-blast (Addgene #83480) backbone ...
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bioRxiv - Genomics 2022Quote: All CRISPRi effectors were cloned into a lentiviral backbone containing a ubiquitous chromatin opening element and a spleen focus forming virus (SFFV) promoter (pMH0001, Addgene # 85969). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragments are then cloned into a mammalian expression vector containing Flag and mEGFP (N- or C-terminal) (modified from Addgene #32104) using NEBuilder HiFi DNA Assembly kit (E2611) ...
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bioRxiv - Cell Biology 2022Quote: ... Oligonucleotide containing the CRISPR target sequence was annealed and ligated into Bbs1 linearized pSpCas9(BB)-2A-Puro (PX459) vector (Addgene #48139). HepG2 cells were transfected with PX459-p62 with X-tremeGENE HP transfection reagent (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... that was first PCR amplified using custom primers (see Table 2) from a plasmid containing lexAop2 (lexAop2-myr-4xSNAPf, RRID: Addgene 87638) and then restriction digested using HindIII and PspXI (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The dual guide backbone1-tRNA cassette was synthesised (Gblock, IDT) and amplified with primers containing the two guideRNA target sites followed by Gibson cloning into pX458 (Addgene #48138). Plasmids were transfected into the KOLF2_C1 hiPSC line with TransIT-LT1 (Mirus Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... were cloned into a Cas9-T2A-Puromycin expressing plasmid containing the U6-gRNA scaffold (gift of A. Németh; Addgene plasmid, 101039). 8 μg of plasmid was used to transfect mESCs using the Promega FuGENE 6 transfection kit ...
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bioRxiv - Microbiology 2024Quote: ... The new product replaced an existing gene when inserted into pcDNA3.1 containing a triple N-FLAG-tag between KpnI and EcoRI (Addgene plasmid #67788). The GFP transfection control plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... an ORF containing the mCherry coding sequence was generated by PCR using pSJ1321 (a gift from Sue Jaspersen73, Addgene plasmid #86413) as a template and primers with BamHI and PacI restriction enzyme recognition sites ...
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bioRxiv - Cell Biology 2024Quote: ... Fragment containing the RFP dropout cassette and the fragment containing the RFP cassette along with the mpknot motif were separately amplified from pU6-pegRNA-GG-aceptor (Addgene #132777) and pU6-tmpknot-GG-acceptor (Addgene #174039) ...