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Citations for Addgene :
1201 - 1250 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Human iPSCs were dissociated to single cells and nucleofected with Cas9-coding plasmid (hCas9, Addgene 41815), sgRNA plasmid and donor plasmid on Amaxa 4D-Nucleofactor program CA-137 (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: U2OS cells were cultured and transfected with 100-200 ng of mEmerald-Tomm20 plasmid (Addgene, 54281) directly on the BIO-133 bottomed well plate ...
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bioRxiv - Bioengineering 2021Quote: CRISPR knockdown cells were generated using the lentiCRISPRv2 system (gift of F. Zheng, Addgene plasmid #52961). Scramble guideRNA (gRNA ...
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bioRxiv - Cancer Biology 2021Quote: Cell lines stably expressing Cas9 were generated by lentiviral infection with pCRISPRV2-FLAG-CAS9 (Addgene #52961) followed by puromycin selection and monoclonal population generation by limiting dilution in 96 well plates ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected with transgene plasmid together with lentivirus packaging plasmids psPAX2 (Addgene, Cat. No. 12260) and pMD2.G (AddGene ...
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bioRxiv - Cancer Biology 2020Quote: ... We enriched RM1-BoM1 cells negative for GFP and transduced with Tdtomato (LeGO-T2, Addgene #27342) and luciferase (pENTR-LUC ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1 cells were stably transduced with lentivirus from lentiCas9-Blast (Addgene #52962, gift from Feng Zhang) and subsequently selected using blasticidin ...
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ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signalingbioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with plasmid encoding ACE2 and packaging vectors (pMD2.G and psPAX2, Addgene) using calcium phosphate transfection method ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral particles were generated by transfecting HEK293T cells with the packaging plasmids pMD2.G (Addgene 12259) and psPAX2 (Addgene 12260 ...
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bioRxiv - Cancer Biology 2020Quote: HeLa cells were infected with lentivirus carrying the Cas9-BFP (blue fluorescent protein) vector (Addgene 52962). HCT116 were transfected with the same vector using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with the pLenti-PGK-Tacc3 or pLKO.1 TRC (control, Addgene 10879), pMD2G (Addgene 12259 ...
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bioRxiv - Biophysics 2020Quote: ... we stably integrated tdMCP-tdGFP and OsTIR1 into cells using a standard lentiviral production protocol (Addgene). For lentivirus production ...
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bioRxiv - Cancer Biology 2021Quote: ... A doxycycline-inducible Cas9 clonal cell line of NALM-6 (using plasmid pCW-Cas9, Addgene #50661) was generated ...
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bioRxiv - Cell Biology 2021Quote: ΦNX-ecotropic packaging cells were transfected with pBABE-hygro (Addgene plasmid # 1765; empty or Bcl-XL) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus was generated in HEK293T cells transfected with pMD2.G and psPAX2 (Addgene, 12259 and 12260) and the given pTRIPZ constructs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated at 50% confluency and transfected the next day with GFP-LC3 (Addgene #21073) or double transfected with GFP-LC3 and one of the FLAG-hSynj1 constructs (see below) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus preparation for stable cell line generation was done with pMD2.G envelope plasmid (Addgene #12259) and psPAX2 packaging plasmid (Addgene 12260 ...
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bioRxiv - Cancer Biology 2021Quote: ... and pCMV-VSV-G/pCMV-dR8.2 for human cell lines (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454 and Addgene plasmid #8455 ...
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bioRxiv - Cancer Biology 2021Quote: ... or RUNX1ΔEx6 amplified from cDNA obtained from 293T cells and the pINDUCER-21-RUNX1 plasmid (Addgene plasmid #97043 ...
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bioRxiv - Cell Biology 2022Quote: ... iEC-ESCs and iHUF cells were tagged with Azurite blue using pLV-Azurite (Addgene plasmid 36086). Lentiviral transductions were performed according to manufacturers’ protocols and successfully tagged cells were further sorted on a Beckman Coulter MoFlow Astrios (Indianapolis ...
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bioRxiv - Molecular Biology 2022Quote: ... cells in each well were transfected with 500ng pCMV-PE2 (Anzalone et al., 2009, Addgene#132775), 330ng pU6-pegRNA and 170ng pBPK1520-ngRNA for PE3 strategy or 500ng pCMV-PE2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviruses were produced in 293T cells with a second-generation packaging system containing psPAX2 (#12260; Addgene) and pMD2.G (#12259 ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293T cells were transfected with pCMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid # 8454) (22) ...
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bioRxiv - Microbiology 2022Quote: ... This was done by transfection of HEK-293 cells with an optimized T7 plasmid (Addgene 65974) together with the antigenomic plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were then infected with viral particles carrying a catalytically dead Cas9-VP64 (Addgene #61425) and MS2-P65-HSF1 constructs (Addgene #89308) ...
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bioRxiv - Neuroscience 2020Quote: ... Lentiviral packaging was performed in HEK 293T cells using commercial plasmids (Addgene plasmids 12259 and 12263) and protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... 6419c5 and 5074 cells were transduced with a lentiviral expression vector of h2b-dendra2 (Addgene #51005). Single cell clones expressing Dendra2-H2B were derived by FACS sorting.
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bioRxiv - Cancer Biology 2021Quote: ... Parental and BRCA2−/− DLD1 Cas9 cells were generated through viral infection with lentiCas9-Blast (Addgene #52962) followed by blasticidin selection ...
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bioRxiv - Physiology 2021Quote: ... a biosensor for cAMP in mammalian cells was purchased from Addgene (Harada, Ito et al. 2017) (Cat #102356) ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T cells were co-transfected with the pcw107-V5 plasmid expressing CA-STAT3 (Addgene #64611), VSV-G envelope plasmid ...
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bioRxiv - Immunology 2022Quote: Lentiviral particles were produced in HEK293T cells by co-transfecting packaging vector psPAX2 (8µg, Addgene, #12260), envelop vector pMD2.G (4µg ...
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AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermisbioRxiv - Developmental Biology 2022Quote: ... Packaging of lentivirus was performed using 293FT cells and pMD2.G and psPAX2 helper plasmids (Addgene plasmids #12259 and #12260 ...
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bioRxiv - Cancer Biology 2022Quote: Cas9 expressing cells were created by transduction of lentiCas9-Blast (a gift from Feng Zhang, Addgene plasmid #52962 ...
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bioRxiv - Cancer Biology 2022Quote: ... HS-SY-II and SYO1 synovial sarcoma cell lines were transduced with lentiCas9-Blast85 (Addgene, #52962) and selected using 20 μg/ml of blasticidin to generate stable Cas9-expressing cell lines ...
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bioRxiv - Molecular Biology 2020Quote: 1×106 cells of WTC p42 were transfected with 5mg AAVS1-TALEN R plasmid (Addgene #59026), 5 μg AAVS1-TALEN L plasmid (Addgene #59025) ...
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bioRxiv - Cancer Biology 2020Quote: ... wild-type HeLa and MCF10A cells were infected with the dCas9 lentiviral construct (Addgene 61425-LV) and selected with 3μg/ml blasticidin for 5 days ...
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bioRxiv - Immunology 2020Quote: ... and lentiviral particles were generated in 293T cells (ATCC) using packaging plasmids pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blasticidin-resistant cells were subsequently transduced with viral supernatant containing LentiGuide-Puro plasmid (Addgene plasmid #52963) with ligated selected sgRNA ...
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bioRxiv - Microbiology 2021Quote: ... subconfluent HEK293T cells were cotransfected with lentiviral genome (psPAX2) (gift from Didier Trono (Addgene plasmid # 12260), with an envelope coding plasmid (pMD2G-VSVG or SARS-CoV-2 Spike protein (HDM_IDTSpike_fixK (kind gift from Bloom’s laboratory ...
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bioRxiv - Biochemistry 2020Quote: ... cell transfection method as described previously (Purroy et al., 2018) using the plasmids pMD2.G (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260 ...
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bioRxiv - Cell Biology 2021Quote: Early passage HeLa-M cells were transfected with 1.5 µg of px459 plasmid (Addgene plasmid #62988) containing a single guide RNA (sgRNA ...
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bioRxiv - Biophysics 2021Quote: ... BL21 AI cell was co-transformed with MutS-bio and BirA expression plasmid (Addgene plasmid #20857)70 and was grown in LB containing 50 μg/ml kanamycin ...
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bioRxiv - Cancer Biology 2021Quote: Cell lines for use in orthotopic in vivo experiments were labeled with pHIV-luc-ZsGreen (Addgene). Bioluminescent imaging was performed every 3-7 days following intraperitoneal injection of D-luciferin and measured using the Xenogen IVIS Spectrum in vivo imaging system (PerkinElmer) ...
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bioRxiv - Biophysics 2020Quote: ... HEK293T cells on the wells were co-transfected with 1250 ng of ACE2 (Addgene plasmid #141185) and 1250 ng of TMPRSS2 expression plasmids (Addgene plasmid #145843 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a pCMV-eNpHR3.0-EYFP plasmid derived from pCaMKIIa-eNpHR3.0-EYFP (Addgene #26971). For this construct ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA plasmid library was packaged in 293FT cells after co-transfection with psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... the cells were transduced by EF1a-CasRx (no NLS-RfxCas13d)-2A-EGFP (modified from Addgene #109049) lentivirus ...
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bioRxiv - Immunology 2020Quote: ... 2 × 104 HEK293T target cells transfected with 500 ng of a human ACE2 expression plasmid (Addgene) were seeded in a white flat-bottom 96-well plate one day prior to the assays ...
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bioRxiv - Developmental Biology 2022Quote: Lentivirus was generated in HEK293T cells using a second-generation system with pMD2.G (Addgene 12259) and psPAX2 (Addgene 12260) ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cells were transfected with the HDR template and pCAG-SpCas9-GFP-U6-gRNA (Addgene: 79144) expressing a gRNA targeted near the stop codon of PGC-1β ...