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Citations for Addgene :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: DD-HA-ER-I-PpoI (a gift from Michael Kastan, Addgene: 49052) was subcloned into a pLX303 backbone vector (a gift from Mikko Taipale ...
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bioRxiv - Molecular Biology 2024Quote: ... The G418 (Geneticin) resistance gene was subcloned from pDEST-CMV-C-eGFP (a gift from Robin Ketteler, Addgene: 122844) and inserted into pLX303-DD-HA-ER-I-PpoI using In-Fusion cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... eGFP vectors were cloned using Gateway cloning from pDONR221-eGFP (a gift from David Root, Addgene plasmid # 25899) and pFRT-TO-DEST (a gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... and pFRT-TO-DEST (a gift from Dr. Thomas Tuschl, Addgene plasmid # 106348). An NLS was then added to eGFP using standard PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... was subcloned into a pLX303 backbone vector (a gift from Mikko Taipale, Addgene: 154472) using In-Fusion cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... 10xHis-UFM1ΔSC was subcloned from pRK5-HA-UFM1 (a gift from Yihong Ye, Addgene: 134639) using PCR and inserted into a modified Lenti-Guide-Puro GFP by replacing the puromycin selection marker with 10xHis-UFM1ΔSC using In-Fusion cloning ...
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bioRxiv - Neuroscience 2024Quote: ... DREADD+ group) was bilaterally injected in the dDG with pAAV5-CaMKIIa-hM4D(Gi)-mCherry (virus titer ≥ 3×1012 vg/ml, Addgene, North Carolina, USA). Inhibitory DREADDs are activated by the artificial ligand clozapine-N-oxide (CNO ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-DIO-hM3D(Gq)-mCherry and pAAV-hSyn-DIO-mCherry were gifts from Bryan Roth 115 with viral preps purchased from Addgene (http://n2t.net/addgene:44361 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-DIO-hM3D(Gq)-mCherry and pAAV-hSyn-DIO-mCherry were gifts from Bryan Roth 115 with viral preps purchased from Addgene (http://n2t.net/addgene:44361, RRID: Addgene 44361; http://n2t.net/addgene:50459; RRID:Addgene 50459). In all cases ...
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bioRxiv - Neuroscience 2024Quote: ... Either AAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (n = 7, 200 nL, 3 × 1012 GC/mL, Serotype: 9; Addgene, MA, USA) to express channelrhodopsin (ChR2 ...
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bioRxiv - Neuroscience 2024Quote: ... to express channelrhodopsin (ChR2) or control virus (n = 4, AAV-Ef1a-fDIO-mCherry, 200 mL, 3 × 1012 GC/mL; Serotype: 9; Addgene) was unilaterally injected over 10 minutes into the MePD using a 2-µL Hamilton microsyringe (Esslab ...
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bioRxiv - Neuroscience 2024Quote: The following rAAV vectors were used: AAV5.hSyn.DIO.hM3D(Gq).mCherry (Addgene 44361 from Bryan Roth (34), titer ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 (Addgene 100845 from Douglas Kim and the GENIE project (36) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1.TREtight.mTagBFP2.B19G (Addgene 100799 from Ian Wickersham (38), titer ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1.syn.FLEX.splitTVA.EGFP.tTA (Addgene 100798 from Ian Wickersham (38), titer ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2.FLEX.DTR.GFP (Addgene 124364 from Eiman Azim and Thomas Jessel (35) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV8.hSyn.DIO.hM4D(Gi) (Addgene 44362 from Bryan Roth (34), titer ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1.hSyn.Cre.WPRE.hGH (Addgene 105553 from James M ...
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bioRxiv - Neuroscience 2024Quote: ... pSADdeltaG.mCherry (Addgene 32636 from Edward Callaway (39), titer ...
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bioRxiv - Microbiology 2024Quote: ... We first cloned the desired inserts (i.e., promoters and mNeonGreen fluorophore) into the multiple cloning site of a pJM220 mini-Tn7 plasmid (Addgene, cat. Number 110559). Fragments were assembled using Gibson assembly (58 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV8-hSyn-hM3D(Gq)-mCherry and AAV8-hSyn-hM4D(Gi)-mCherry are available commercially (gift from Bryan Roth, Addgene plasmids #50474-AAV8 and #50475-AAV8 respectively ...
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bioRxiv - Neuroscience 2024Quote: ... packaged at UPenn Vector Core 2.5 × 1014 GC ml−1] and KORD [AAV8-HSyn-DIO-HA-KORD-IRES-mCitrine (Addgene plasmid no. 65417), packaged at UPenn Vector Core ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV9-CAMKII-mScarlet-C1V1-KV2.1 (Addgene, MA stock # 124650) mixed 1:1 and diluted to a titer of ∼1x1012 vg/mL using sterile PBS ...
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bioRxiv - Neuroscience 2024Quote: ... containing AAV1-synapsin-l-GCamp6f (Addgene, MA stock #100837 (44)) and AAV9-CAMKII-mScarlet-C1V1-KV2.1 (Addgene ...
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Real-time activity of dynorphin-expressing neurons in mouse central amygdala during alcohol drinkingbioRxiv - Neuroscience 2024Quote: Mice underwent unilateral surgical delivery of a Cre-dependent GCaMP7f calcium sensor into the CeA (pGP-AAV1-syn-FLEX-jGCaMP7f-WPRE, a gift from Douglas Kim & GENIE Project, Addgene viral prep # 104492-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... University of Pennsylvania vector core) and AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 (0.4 μl, 1.3 x 1013 GC/ml, Addgene) was injected into the IL ...
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bioRxiv - Neuroscience 2024Quote: ... serotype 9 (AAV9) expressing GCaMP6s (AAV9.CAG.GCaMP6s.WPRE.SV40, Addgene, USA) was injected subcutaneously in the nape of the neck of pups (P2-P6) ...
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bioRxiv - Neuroscience 2024Quote: ... 85 nl of AAV1-hSyn:Cre (Addgene, 2 × 1013) was injected into vCA1 and 200 nl of a 1:1 cocktail of AAV9-pMeCP2:DIO-Cas9 (2 × 1012 GC/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/2 (for AAV2; Addgene plasmid # 104963), pAAV2/5 (for AAV5 ...
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bioRxiv - Neuroscience 2024Quote: The pAAV-CMV:Cas9-T2A-mCherry/U6:sgRNA plasmid was modified from pX601 (pAAV-CMV:NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Addgene plasmid # 61591). To visualize the AAV-infected cells ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/5 (for AAV5; Addgene plasmid # 104964), or pAAV2/9n (for AAV9 ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV2/9n (for AAV9; Addgene plasmid # 112865) were co-transfected into HEK-293T cells using PEI-Max for AAV production ...
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bioRxiv - Neuroscience 2024Quote: ... which is a restriction enzyme digested product of pAAV-Ef1a:DIO-EYFP (Addgene plasmid # 27056), were then ligated using T4 DNA ligase (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... AAV5- CAG-GFP was a gift from Edward Boyden (Addgene viral prep #37825-AAV5 ...
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bioRxiv - Molecular Biology 2024Quote: ... pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67, Addgene plasmid # 49386) followed by insertion into PCR linearised pRRL backbone ...
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bioRxiv - Molecular Biology 2024Quote: Lentivirus particles for transduction of IMR90 E6E7 hTERT cells were produced by transfection of an expression plasmid with packaging and envelope plasmids gifted from Didier Trono (Addgene plasmid #12260) and Robert A ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1.5 µg of the pMD2.G plasmid (Addgene #12259) were mixed with 13 µl Lipofectamine 3000 and 13 µl P3000 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Inducible Cas9 vector pCW-Cas9-blast and gRNA expression vector pLentiGuide-puro were purchased from Addgene. Oligonucleotide sequences used for gRNA cloning are available upon request ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µg of psPAX2 (Addgene #12260), and 1.5 µg of the pMD2.G plasmid (Addgene #12259 ...
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bioRxiv - Immunology 2024Quote: ... The plasmid pcDNA3.1 SARS-CoV-2 S D614G was a gift from Jeremy Luban (Addgene plasmid # 158075 ...
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bioRxiv - Molecular Biology 2024Quote: ... a pUC18-LucFF plasmid (will be deposited on Addgene) was also generated by replacing the GFP ORF by the firefly luciferase ORF ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with the pCAG-eSpCas9-2A-GFP (generous gift from Jizhong Zou, Addgene plasmid #79145 ...
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bioRxiv - Neuroscience 2024Quote: - AAV8 hSyn-DIO -mCherry (2.3 × 1013 gp/ml) (Addgene #50459)
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bioRxiv - Neuroscience 2024Quote: ... the ires-CreERT2 cassette (CreERT2 was derived from plasmid #46388, Addgene) was inserted just after the coding end of exon 12 using the homologous recombination-based method ...
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bioRxiv - Neuroscience 2024Quote: - AAV2retro hSyn.Cre.WPRE.hGH (1.8 × 1013 gp/ml) (Addgene #105553)
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bioRxiv - Neuroscience 2024Quote: ... the ires2-Cre cassette (ires2 was derived from plasmid #159600, Addgene) was inserted just after the coding end of exon 3 using the microhomology-mediated end joining (MMEJ)- based method ...
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bioRxiv - Neuroscience 2024Quote: - AAV8 hSyn-DIO-hM3D(Gq)-mCherry (2.1 × 1013 gp/ml) (Addgene #44361)
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bioRxiv - Neuroscience 2024Quote: ... was utilized to administer 500 or 100 nl of AAVrg hSyn-Cre-WPRE-hGH (Addgene #105553) with 0.1% Fast Green (#F7252 ...
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bioRxiv - Neuroscience 2024Quote: The following AAV vectors were obtained from Addgene:
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bioRxiv - Neuroscience 2024Quote: ... DV: -4.4 mm relative to bregma) microinjection of either AAV5.hSyn::DIO-mCherry (Addgene, titer ...