Labshake search
Citations for Addgene :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... the pYPQ265E2 (Addgene # 164719) was used for generating attL1-attL5 Cas12a entry clones ...
-
bioRxiv - Molecular Biology 2024Quote: ... 7.5 μg packaging helper plasmid (psPAX2, Addgene #12260), 5 μg envelope helper plasmid (pMD2.G ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μg envelope helper plasmid (pMD2.G, Addgene #12259) and 80 μl of 1mg/ml polyethylenimine (PEI ...
-
bioRxiv - Molecular Biology 2024Quote: ... Final plasmid was named p5E-UBI-loxTagBFP (Addgene #172518) and recorded in our database as 50_p5E-UBI-loxTagBFP.
-
bioRxiv - Molecular Biology 2024Quote: ... a custom R4-R2_destination clone named 1456-pDEST-minTol2_R4-R2_Cryst-eGFP (Addgene #171795 ...
-
bioRxiv - Molecular Biology 2024Quote: ... we gel extracted the 3x polyA sequence present on plasmid pENTR5’_ubi:loxP-EGFP-loxP (Addgene # 27322) and inserted it in the NotI-dephosphorylated p5E-UBI_Lflox_BFP plasmid through T4 ligation ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR product was further digested using BamHI along with tol2kit 101_p5E-Ubiquitin plasmid (Addgene #27320). Both were purified and 101_p5E-Ubiquitin was dephosphorylated using NEB Antarctic phosphatase following manufacturer instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... was mixed with our 469-pME-iCre (Addgene #171792) and either with 101_p5E-Ubiquitin ...
-
bioRxiv - Molecular Biology 2024Quote: UBI:loxBFP:mKate:INTRON_miRsmn1-4141 was cloned using a Gateway LR-reaction mixing destination p5E-UBI-loxTagBFP (Addgene #172518) with our 010-pME-mKate2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... a modified form of Lentiguide-Puro (Addgene #52963), in which Cas9 was replaced with NLS-tagged GFP or mCherry by ligation into AgeI and BamHI sites ...
-
bioRxiv - Molecular Biology 2024Quote: ... All plasmids used in this study are available from Addgene.
-
bioRxiv - Molecular Biology 2024Quote: ... the SMARCAL1 coding sequence was cloned into pLEX_305-N-dTAG (Addgene #91797) by the Gateway system (Life Technologies/Thermo Fisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... mCherry-LaminB1-10 was a gift from Michael Davidson (Addgene plasmid # 55069 ; http://n2t.net/addgene:55069; RRID:Addgene_55069). For oligonucleotide sequences see Supplementary Table 3.
-
bioRxiv - Molecular Biology 2024Quote: pPAGFP-C1 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11910 ...
-
bioRxiv - Immunology 2024Quote: ... using the SARS-CoV1 SPIKE plasmid pcDNA3.3_CoV1_D28 (Addgene plasmid # 170447)39 ...
-
bioRxiv - Molecular Biology 2024Quote: ... We use pCMV-VSVG (#8454, Addgene) and HELPER (a gift from Dr ...
-
bioRxiv - Molecular Biology 2024Quote: ... The following retroviral vectors were used for cellular transformation: pWZL-hygro E1A (#18748, Addgene), pWZL-Blast RASGV12 (#12277 ...
-
bioRxiv - Molecular Biology 2024Quote: ... We transfected 300,000 cells with 1.5 µg of flippase-encoding plasmid (Addgene #13787) and 0.5 µg of pLD037 using lipofectmamine 2000 (Invitrogen ...
-
Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... or coilin-GFP plasmid (Addgene) using Opti-MEM Reduced Serum Medium (Gibco ...
-
bioRxiv - Molecular Biology 2024Quote: ... pYPQ132B (Addgene # 69282), pYPQ133B (Addgene # 69283 ...
-
bioRxiv - Molecular Biology 2024Quote: ... pYPQ133B (Addgene # 69283) and pYPQ134B (Addgene # 179216 ...
-
bioRxiv - Genomics 2024Quote: ... the pIS-0 vector48 (RRID: Addgene_12178, Addgene, Cambridge, MA) was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB) ...
-
bioRxiv - Genomics 2024Quote: XLone-GFP was a gift from Xiaojun Lian (Addgene plasmid # 96930; http://n2t.net/addgene:96930; RRID:Addgene_96930) (35) ...
-
bioRxiv - Genomics 2024Quote: XLone-GFP was a gift from Xiaojun Lian (Addgene plasmid # 96930 ...
-
bioRxiv - Molecular Biology 2024Quote: Plasmid pSpCas9(BB)-2A-GFP (PX458) was obtained from Addgene #48138 (Watertown ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cargo RNA expression plasmids for mCherry-MS2×8 and Cre-MS2×8 were pFH2.22 (Addgene, #205537) and pJAM1.52 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The RNA exporter EPN24-MCP-T2A-EGFP expression plasmid was pFH2.105 (Addgene, #205550). Cargo RNA expression plasmids for mCherry-MS2×8 and Cre-MS2×8 were pFH2.22 (Addgene ...
-
bioRxiv - Immunology 2024Quote: 8 x 105 293T cells were seeded in 6-well plate and transfected with pcDNA3-FLAG-VSVG plasmids (Addgene, plasmid 80606) for 24 hours with 50 μl of purchased or previously collected VSVΔG-Luc pseudovirus (Kerafast ...
-
bioRxiv - Immunology 2024Quote: The human Calabrese CRISPR activation pooled library was a gift from David Root and John Doench (Addgene 92379, 92380). To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs ...
-
bioRxiv - Immunology 2024Quote: ... that itself was a polycistronic derivative of Feng Zhang’s dual vector system (Addgene 61422, Addgene, 73797). Successive daughter plasmids were generated via simple ligation or Gibson assembly using annealed complementary oligonucleotides (Integrated DNA Technologies ...
-
bioRxiv - Immunology 2024Quote: ... Sequencing indicated recoveries of 99.997% (AUC = 0.62) and 99.986% (AUC = 0.64) of subcloned Calabrese half-library A (AUC = 0.59, Addgene) and B (AUC = 0.61 ...
-
bioRxiv - Immunology 2024Quote: ... and B (AUC = 0.61, Addgene) respectively (Fig S10i) ...
-
bioRxiv - Molecular Biology 2024Quote: ... the four sgRNA cassettes were assembled into the attL5-attL2 vector pYPQ144 (Addgene # 69296) using Golden Gate assembly 58 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The annealed dsDNA was sub-cloned into the BsmbI-digested lentiviral sgRNA-EFS-GFP/mCherry vector under the control of U6 promoter (LRG, Addgene #65656). To improve the transcription efficiency ...
-
bioRxiv - Molecular Biology 2024Quote: A pET28b plasmid encoding C-terminal 6xHis-tagged nuclear Pif1 (Addgene plasmid #65047)64 was employed to express and purify Pif1 from E ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The plasmid backbone pAM552G (35, 47) (Addgene accession #154131) was used for testing ribosome variants from RISE in vivo (see below) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... pJL1sfGFP (encoding the reporter sfGFP under a T7 promoter, Addgene accession #102634) and pRDV3xFLAG-TolA-HH (encoding the 3xFLAG tag for ribosome selection under a T7 promoter ...
-
bioRxiv - Synthetic Biology 2024Quote: A plasmid containing GCaMP6m was obtained from Addgene (36) and gblocks were obtained from IDT ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (Addgene viral prep #1120050-AAV5) was injected into layer 2/3 of the primary somatosensory barrel (S1BF ...
-
bioRxiv - Neuroscience 2024Quote: ... To monitor astrocytic Ca2+ signaling we used the genetically encoded Ca2+ indicator (GECI) AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925) (viral injections of 0.6 µl ...
-
bioRxiv - Neuroscience 2024Quote: ... into the DR and bilateral infusions of AAVretro-hSyn-DIO-EGFP (200 nL over 2 min; 1.3 x 1013 GC/mL, Addgene) into the BLA ...
-
bioRxiv - Neuroscience 2024Quote: ... mCherry as a temperature sensor was delivered to neurons via AAV9 viral vector transfection (Addgene, pAAV-hSyn-mCherry, 1E10 vp/dish). The viral particles were added to neurons at 3 days in vitro ...
-
bioRxiv - Neuroscience 2024Quote: ... Syn-driven GCaMP6f as a calcium sensor was delivered to neurons via AAV9 viral vector transfection (Addgene, pAAV.Syn.GCaMP6f.WPRE.SV40 ...
-
bioRxiv - Neuroscience 2024Quote: ... pCAG mt-SypHer was created by PCRing the DNA encoding mt-SypHer from Addgene plasmid #48251 (a gift from Nicolas Demaurex) ...
-
bioRxiv - Neuroscience 2024Quote: ... a gift from Masamitsu Iino (Addgene (USA) plasmid #58215)(Suzuki et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 400 nL of AAV1-hSyn1-SIO-stGACR2-FusionRed (Addgene 105677-AAV1; titer: 1.0 x 1012 vg/mL) or AAV1-CAG-FLEX-tdTomato (Addgene 28306-AAV1 ...
-
bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 400 nL of AAV5-hSyn-DIO-EGFP (Addgene 50457-AAV5; titer: 1.3 x 1012 vg/mL) into the right BLA at coordinates AP ...
-
bioRxiv - Neuroscience 2024Quote: ... pCAG 4xmt-iATPSnFR1.0 was created by PCR of the DNA encoding iATPSnFR1.0 from Addgene Plasmid #102556 (a gift from Baljit Khakh) ...
-
bioRxiv - Neuroscience 2024Quote: ... we intracranially injected 300 nL of AAV5-EF1a-Nuc-flox(mCherry)-EGFP (Addgene 112677-AAV5; titer:) into the right NAcSh at coordinates ...
-
bioRxiv - Neuroscience 2024Quote: ... pAAV5-hSyn-DIO-mCherry (Addgene, 50459, vg/mL), pAAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene ...