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Citations for Addgene :
951 - 1000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 500 nl of AAV5-EF1a-DIO-ChR2(H134R)-mCherry virus (Addgene #44361-AAV5) was injected unilaterally at 100nl/min ...
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bioRxiv - Neuroscience 2024Quote: ... plus either AAV8-hSyn-DIO-hM4Di-mCherry (2.2 x 1013 GC/mL, Addgene, Watertown, MA) or AAV8-hSyn-DIO-mCherry (Control virus ...
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bioRxiv - Neuroscience 2024Quote: ... The APP gRNA sequence was cloned into pSpCas9 (BB)-2A-Puro (PX495) v.2.0 (plasmid #62988; Addgene, Cambridge, MA, USA) using the method described by Ran et al.48 ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAX APP Swe/Ind (#30145, Addgene), respectively ...
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bioRxiv - Microbiology 2024Quote: ... cells was transfected with 15ng STING (pMSCV-hygro-STING R232, Addgene 102608), 20ng encoding firefly luciferase driven by an IFN-beta promoter (IFN-Beta_pGL3 ...
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bioRxiv - Molecular Biology 2024Quote: ... pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051; http://n2t.net/addgene:79051; RRID:Addgene_79051, and Addgene plasmid # 79050; http://n2t.net/addgene:79050 ; RRID:Addgene_79050). pET30a-AlkB-D135S/L118V (20 ...
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bioRxiv - Molecular Biology 2024Quote: ... pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051; http://n2t.net/addgene:79051; RRID:Addgene_79051, and Addgene plasmid # 79050 ...
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bioRxiv - Microbiology 2024Quote: ... obtained from the pAG25 plasmid (Addgene plasmid #35121), flanked with sequences of the target allele ...
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bioRxiv - Microbiology 2024Quote: ... GOI_gRNA2_F and GOI_gRNA2_R) containing gRNA sequence were annealed and cloned into BsaI site of the pU6-Universal vector (Addgene #52694). To generate a construct for deleting the entire coding region of GOI ...
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bioRxiv - Molecular Biology 2024Quote: ... pLX209-neo-WRN K577M were a gift from Francisca Vazquez (respectively, Addgene plasmids # 125788, http://n2t.net/addgene:125788; RRID:Addgene_125788 ...
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bioRxiv - Molecular Biology 2024Quote: ... pLX209-neo-WRN K577M were a gift from Francisca Vazquez (respectively, Addgene plasmids # 125788 ...
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bioRxiv - Cell Biology 2024Quote: Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid #45203 ...
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bioRxiv - Cell Biology 2024Quote: Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid #45203; http://n2t.net/addgene:45203; RRID:Addgene 45203). Cells were transiently transfected with a and plated onto glass-bottom plates ...
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bioRxiv - Molecular Biology 2024Quote: ... SK-BR-3 HER2-knockout (SK-BR-3 KO) were obtained using pSpCas9 BB-2A-Puro (PX459) V2.0 (9200 bp, Addgene) containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’ ...
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bioRxiv - Molecular Biology 2024Quote: Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene#1000000096) was amplified in E coli ...
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bioRxiv - Genetics 2024Quote: ... γcry1:BFP vector (Addgene #117789) with primers Ori-F and 2A-R (35 ...
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bioRxiv - Neuroscience 2024Quote: ... and hOPTNΔC (Addgene, #23053) were cloned into meGFP-hTRAK1 to generate meGFP-hOPTN and meGFP- hOPTNΔC ...
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bioRxiv - Neuroscience 2024Quote: ... TRAK1 (Addgene, #127621), his-hOPTN (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... 4xmts-mScarlet-I (Addgene, #98818), TurboID (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251) ...
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bioRxiv - Neuroscience 2024Quote: ... TurboID (Addgene, #107169) and Cre 33 were cloned and packaged into our pAM-AAV- mSncg-WPRE backbone containing the RGC-specific full-length mSncg promoter 33 ...
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bioRxiv - Microbiology 2024Quote: ... a 350-450 bp fragment corresponding to the U16 sequence was cloned into the double T7 vector L4440 (a gift from Andrew Fire, Addgene plasmid # 1654). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... We acquired wild-type α-syn/pET21a (Addgene 51486) courtesy of the Michael J ...
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bioRxiv - Microbiology 2024Quote: ... The Ebola GP-encoding plasmid was obtained from Addgene. Information about RBPs is provided in Table S1.
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bioRxiv - Microbiology 2024Quote: ... Guide RNAs targeting exon 6 of Akr1c13 were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230). A T7-sgRNA PCR product was amplified and in vitro transcribed as previously described (65 ...
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bioRxiv - Molecular Biology 2024Quote: Lentiviruses were produced in HEK293FT cells cultured in T225 flasks by cotransfection of 30μg of packaging plasmid (psPAX2, Addgene #12260), 30 μg of envelope plasmid (VSV-G ...
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bioRxiv - Microbiology 2024Quote: ... a gift from Didier Trono (Addgene plasmid #12260 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid #47929; http://n2t.net/addgene:47929; RRID: Addgene_47929). pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ...
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bioRxiv - Molecular Biology 2024Quote: The plasmid pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro (Addgene plasmid #141387 from Nevan Krogan) [12] was used for the overexpression of the accessory proteins ORF6 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-BLM is from Addgene plasmid #80070 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid #47929 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542; http://n2t.net/addgene:62542; RRID: Addgene_62542). 250 ng/μl of Cas9 mRNA and 50 ng/μl of gRNA(s ...
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bioRxiv - Molecular Biology 2024Quote: ... and full-length mTagBFP2 in Puc19 (Addgene Plasmid #50005), driven by a SFFV promoter (from plasmid #pFu-62 derived from Addgene Plasmid #57827 ...
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bioRxiv - Molecular Biology 2024Quote: ... and a WPRE sequence in pLentiGuide-Puro (Addgene Plasmid #52963), driven by the EF1a promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... driven by a SFFV promoter (from plasmid #pFu-62 derived from Addgene Plasmid #57827 ...
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bioRxiv - Molecular Biology 2024Quote: The expression vectors encoding truncated AlkB and AlkB D135S (13) pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051 ...
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bioRxiv - Molecular Biology 2024Quote: ... pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051; http://n2t.net/addgene:79051; RRID:Addgene_79051 ...
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bioRxiv - Cell Biology 2024Quote: ... the primers 5’-CACCGCATCGCTCCTGCGTCCGCCA-3’ and 5’-AAACTGGCGGACGCAGGAGCGATGC-3’ were annealed and subcloned into px458-pSpCas9(BB)-2A-GFP (Addgene) using the BpiI restriction site to generate a guide RNA ...
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G plasmid (Addgene, Plasmid # 12259). The conditioned media was collected and filtered 48h post transfection and used to infect the Kdm4a-null MEFs using polybrene (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... PAX2 plasmid (Addgene, Plasmid # 35002) and pMD2.G plasmid (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding region of SERCA2b (Addgene, plasmid #75188 ...
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bioRxiv - Microbiology 2024Quote: ... The two fragments upstream and downstream of the mfd gene were cloned at each side of the chloramphenicol cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... pN3-3×Flag-Control was purchased from Addgene (Watertown ...
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bioRxiv - Molecular Biology 2024Quote: ... 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg of envelope plasmid (VSV-G, Addgene #8454), and 30 μg of transfer plasmid using 270 μl of polyethylene imine (PEI) ...
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bioRxiv - Molecular Biology 2024Quote: ... hfRfxCas13 constructs were subcloned from Addgene #190034 ...
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bioRxiv - Microbiology 2024Quote: ... a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID: Addgene_12260), to generate lentiviral particles ...
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bioRxiv - Molecular Biology 2024Quote: To generate RAD54L2 knockouts a double-stranded DNA oligo encoding a sgRNA targeting the RAD54L2 5’ region from the TKOv3 library (Hart et al, 2017) (5’-AAGATGGGCAGCAGCCGCCG CGG–3’) was cloned into pSpCas9(BB)-2A-Puro (PX459) (RRID: Addgene_48139) to yield PX459-RAD54L2.
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bioRxiv - Microbiology 2024Quote: ... 20ng encoding firefly luciferase driven by an IFN-beta promoter (IFN-Beta_pGL3, Addgene 102597), 5ng of renilla luciferase (pRL-TK - Promega E2241) ...