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Citations for Addgene :
6851 - 6900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
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bioRxiv - Synthetic Biology 2023Quote: ... the BBR1 origin of replication was amplified from pSEVA331Bb (Addgene #78269). A silent mutation was introduced in the Rep protein to remove a BbsI restriction site (G186A) ...
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bioRxiv - Microbiology 2023Quote: ... The coding sequence for the α-ALFA NB was amplified by PCR from the mEGFP-NbALFA plasmid (Addgene plasmid #159986; (Jin, et al. 2021)) using the primers P3 and P4 ...
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bioRxiv - Neuroscience 2023Quote: ... The following viruses were injected:: AAV5-hSyn-DIO-hM3Dq-mCherry (DREADD-Gq, titer 6 × 1012 cfu/ml, 250ul bilateral, #44361, Addgene), AAV5-hSyn-DIO-mCherry (control ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg-hSyn-DIO-EGFP (1.8 × 1013 vg/ml, 100nl unilateral, #50457, Addgene), AAVrg-Ef1a-fDIO-mCherry (2.2 × 1013 vg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... AAV9-EF1a-fDIO-Cre (1.3 × 1013 vg/ml, 100nl unilateral, #121675, Addgene), AAV5-EF1a-fDIO-mCherry (1 × 1013 vg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the signal peptide (0-26 amino acids) of pro-opiomelanocortin (#176704, Addgene) and ectodomain (52-750 amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... and lentiSAMv2 (Addgene #75112). At 72 hr post-transduction ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type MEFs were transduced with lentiviral particles containing the plasmids lentiMPHv2 (Addgene #89308) and lentiSAMv2 (Addgene #75112) ...
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bioRxiv - Molecular Biology 2023Quote: gRNAs targeting LSM14A (5’-TCTGTACCAAAGGATCGAAC-3’) and XRN1 (5’-AGAGAAGAAGTTCGATTTGG-3’) were cloned into LentiCRISPR v2 (Addgene #52961) using BsmBI restriction enzyme sites and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... pGFPGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64401; http://n2t.net/addgene:64401; RRID:Addgene_64401) (Vickers et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... pMT-Hrp48-APOBEC-P2A-dsRed (pCR8) was made by amplifying rat APOBEC1 from pCMV-BE1 (Addgene #73019) using primers CR26 and CR27 ...
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bioRxiv - Molecular Biology 2023Quote: ... we amplified APOBEC from pCMV-BE1 (Addgene #73019) using primers CR28 and CR27 ...
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bioRxiv - Molecular Biology 2023Quote: Ro-GFP (reduced-oxidized GPP) plasmid targeted to mitochondrial matrix was purchased from Addgene (#49437) and delivered into cells by electroporation ...
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bioRxiv - Molecular Biology 2023Quote: Cepia ER plasmid was purchased from Addgene (#58215). The CEPIA1er protein was excited at 543 nm ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-CKIIa-stGtACR2-FusionRed 34 (Addgene viral prep #105669-AAV1) was infused bilaterally in the ventral subiculum (6.5mm posterior and ± 4.5mm lateral of bregma) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.8 μl of AAV solution containing pZac2.1 gfaABC1D-cyto-GCaMP6f (Addgene viral prep # 52925-AAV5) was injected at 100 nL/min by means of a hydraulic injection apparatus driven by a syringe pump (UltraMicroPump ...
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bioRxiv - Neuroscience 2023Quote: CVS-N2C-Crimson (Addgene #172377).
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bioRxiv - Neuroscience 2023Quote: pAdDeltaF6 (Addgene #112867) – AAV Helper Capsid.
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were co-transfected with 3 plasmids: pAd-DELTA F6 (plasmid No. 112867; Addgene), serotype plasmid AAV PHP.eB ...
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bioRxiv - Neuroscience 2023Quote: ... or VIP-Cre80 mouse lines in combination with the Cre-inducible viral expression of GCaMP6f in the ACC (AAV1/9-SYN-FLEX-GCaMP6f; Addgene, #100833).
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bioRxiv - Neuroscience 2023Quote: Wild-type and Flailer primary neuronal cultures were infected at 3 DIV with AAV coding for GCaMP6s (Addgene #51086) and FL1 or control AAVs ...
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bioRxiv - Neuroscience 2023Quote: ... a plasmid coding for GCamP6s under the CamKIIa promoter in a AAV backbone was obtained from Addgene (Cat #51086).
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bioRxiv - Neuroscience 2023Quote: The generation of plasmids for this assay was previously described and obtained from Addgene [7] ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2) or a GFAP-GFP Adeno-associated virus (AAV) construct (Addgene plasmid #50473) for use in animal experiments (utilized in Figure 3 and Figure 4) ...
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bioRxiv - Neuroscience 2023Quote: ... produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636 ...
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bioRxiv - Neuroscience 2023Quote: ... injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2×1012 to 1×1013 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636 ...
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bioRxiv - Neuroscience 2023Quote: ... Janelia) or AAVretro_Syn_GCamp7f (1.0e13 GC/ml, lot #21720; 1.2e12GC/ml, lot #v52598, Addgene 104488-AAVrg) in the right dorsolateral striatum (DLS ...
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bioRxiv - Neuroscience 2023Quote: A Cre-dependent inhibitory optogenetic construct halorhodopsin (eNpHR, AAV5-Ef1a-DIO eNpHR 3.0-EYFP at titer ≥ 1×10¹³ vg/mL, Addgene plasmid # 26966) or an empty vector (control ...
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bioRxiv - Neuroscience 2023Quote: ... Viral vector sequences are available through commercial suppliers (e.g., Addgene and UNC Vector Core).
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bioRxiv - Microbiology 2023Quote: ... Lentiviral packaging was carried out in HEK293T cells using the 3rd generation packaging plasmids pCMV delat R8.2 (#12263; Addgene) and pCMV-VSVG (#8454 ...
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bioRxiv - Microbiology 2023Quote: ... resistance flanked by the 5’ region of VDU and part of the VDU coding sequence was prepared by PCR using the plasmid pTREX-b-NLS-hSpCas9 (a gift from Rick Tarleton, Addgene plasmid #62543) as template and the donor TcVDU84BlastFOW and donor TcVDU84BlastREV ...
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bioRxiv - Microbiology 2023Quote: ... or pcDNA3-AKT-PH[R25C]-GFP (Addgene #18837), (a gift from Dr ...
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bioRxiv - Microbiology 2023Quote: ... HEK 293Ts were reverse-transfected with pcDNA3-AKT-PH-GFP (Addgene #18836) or pcDNA3-AKT-PH[R25C]-GFP (Addgene #18837) ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg-hSyn-DIO-mCherry (pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth Addgene viral prep # 50459-AAVrg ...
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bioRxiv - Neuroscience 2023Quote: ... AAV9-axon-GCaMP6s-P2A-mRuby3 (pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 was a gift from Lin Tian Addgene viral prep # 112005-AAV9 ...
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bioRxiv - Neuroscience 2023Quote: ... splice acceptor from pSA-βGeo (Addgene plasmid #21709; (62)) ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal area CA1 was injected with a GRABNE NE sensor (AAV9-hSyn-GRAB_NE1m; Addgene #123308; (32)) or GRABDA DA sensor (AAV9-hSyn-GRAB-DA2m ...
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bioRxiv - Neuroscience 2023Quote: ... we subcloned a 33.4 kb fragment from the modified BAC into pMCS-DTA (Addgene plasmid #22730; (65) to generate a targeting vector which extends from 4.9 kb upstream of the loxrox-Hygro/Zeo insertion to 5 kb downstream of the 2ATNSAG insertion.
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bioRxiv - Neuroscience 2023Quote: ... or GRABDA DA sensor (AAV9-hSyn-GRAB-DA2m; Addgene # 140553; (33)) using the following stereotaxic coordinates ...
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bioRxiv - Pathology 2023Quote: ... The library was a gift from Feng Zhang lab (Addgene # 1000000049)58 ...
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bioRxiv - Microbiology 2023Quote: ... transformed with pET-Cas9-NLS-6xHis (Addgene plasmid #62933) (Zuris et al. ...
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bioRxiv - Microbiology 2023Quote: ... Electrocompetent cells containing pREDCas9 (Addgene #71541) were generated as previously described[68] ...
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bioRxiv - Microbiology 2023Quote: ... and inserted into pgRNA (Addgene #44251). To construct the donor DNA ...
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bioRxiv - Neuroscience 2023Quote: ... or rAAV5-hsyn-DIO-mCherry (around 5.0 x 1012 vg/ml for each) (Addgene, Watertown, MA, USA) in the dorsal raphe nucleus [Anterior-posterior (AP) ...
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bioRxiv - Neuroscience 2023Quote: ... O-GECO1-expression vector (#46025) was acquired from Addgene and subcloned into the pLenti-CMV transfer vector ...
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bioRxiv - Neuroscience 2023Quote: All viral vectors were obtained from Addgene. PHP.S-FLEX-tdTomato (28306) ...
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bioRxiv - Neuroscience 2023Quote: ... Viral aliquots were obtained from commercial suppliers (Addgene and UNC Vector Core). Viral vectors were infused using a microsyringe pump system (UMP3T-2 ...
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bioRxiv - Neuroscience 2023Quote: ... Both plasmids were obtained from Addgene as gifts from Dr ...
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bioRxiv - Neuroscience 2023Quote: ... or an empty vector (control, AAV5-Ef1a-DIO EYFP at titer ≥ 1×10¹³ vg/mL, Addgene plasmid # 27056) were utilized ...