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Citations for Addgene :
5501 - 5550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... pU6-sgGFP-NT1 was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid #46914 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pCAG-EGxxFP-Cetn1 was a gift from Masahito Ikawa (Addgene plasmid #50717 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pU6-sgGFP-NT1 was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid #46914; http://n2t.net/addgene:46914; RRID:Addgene_46914)8) ...
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bioRxiv - Neuroscience 2023Quote: ... - and Cre-dependent GCaMP7s (Addgene# 104495-AAV1) - pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104495 ...
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bioRxiv - Neuroscience 2023Quote: ... Wilson (Addgene plasmid # 105558 ...
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bioRxiv - Neuroscience 2023Quote: ... Wilson (Addgene plasmid # 105558 ; http://n2t.net/addgene:105558 ; RRID:Addgene_105558) - and Cre-dependent GCaMP7s (Addgene# 104495-AAV1 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-Lin28b (Golden et al., 2015) or FLAG-LIN28A (Addgene no. 51371) constructs were co-transfected with 3xHA-TRIM71 expressing constructs using Polyethylenimine ...
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bioRxiv - Neuroscience 2023Quote: ... into pCDH-EF1a-eFFly-T2A-mCherry (Addgene, no. 104833) replacing eFFly with restriction enzymes XbaI (NEB ...
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bioRxiv - Physiology 2023Quote: ... 6 μg of psPAX2 (Addgene), and 15 μg of pLV6-BMAL1-Luc vector (Addgene) ...
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bioRxiv - Physiology 2023Quote: ... which was a gift from Feng Zhang (Addgene plasmid #98290). The primers used to design the sgRNA targets were (5’ to 3’ ...
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bioRxiv - Physiology 2023Quote: ... pAAV-EF1α-DIO-hM3Dq-mCherry (2.4×1012 vg/ml, Addgene plasmid #50460-AAV5; http://www.addgene.org/50460/; RRID: Addgene_50460), pAAV-EF1α-DIO-mCherry (3.6×1012 vg/ml ...
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bioRxiv - Physiology 2023Quote: ... pAAV-EF1α-DIO-hM3Dq-mCherry (2.4×1012 vg/ml, Addgene plasmid #50460-AAV5 ...
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bioRxiv - Physiology 2023Quote: ... and 9 μg of psPAX2 (Addgene), 6 μg of psPAX2 (Addgene) ...
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bioRxiv - Physiology 2023Quote: ... pAAV-CAG-dLight1.1 was a gift from Lin Tian (Addgene viral prep # 111067-AAV5; http://n2t.net/addgene:111067; RRID: Addgene_111067)
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bioRxiv - Physiology 2023Quote: ... pAAV-CAG-Flex.GCaMP6f.WPRE (3.15×1013 vg/ml, working dilution 1:10, Addgene plasmid #100835-AAV5; http://www.addgene.org/100835/; RRID:Addgene_100835) was a gift of Douglas Kim and GENIE Project ...
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bioRxiv - Physiology 2023Quote: ... pAAV-GfaACC1D.Lck-GCaMP6f.SV40 (1.53×1013 vg/ml, working dilution 1:5, Addgene plasmid #52925-AAV5 ...
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bioRxiv - Physiology 2023Quote: ... pAAV-CAG-dLight1.1 was a gift from Lin Tian (Addgene viral prep # 111067-AAV5 ...
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bioRxiv - Physiology 2023Quote: ... pAAV-GfaACC1D.Lck-GCaMP6f.SV40 (1.53×1013 vg/ml, working dilution 1:5, Addgene plasmid #52925-AAV5; http://www.addgene.org/52295/; RRID: Addgene_52925) was a gift of Baljit Khak ...
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bioRxiv - Physiology 2023Quote: ... We unilaterally injected 0.6 µl of virus (pAAV.Syn.Flex.GCaMP6f.WPRE.SV40, Addgene viral prep #100833-AAV9 ...
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bioRxiv - Neuroscience 2023Quote: ... one plasmid (px458, Addgene Plasmid #48138) expressing sgRNA as well as Cas9 was used to introduce double-strand-breaks near Exon 7 of SMN2 locus ...
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bioRxiv - Neuroscience 2023Quote: ... The following single-stranded (ss) oligos (IDT) were annealed and cloned into the px458 plasmid (Addgene # 48138) using BbsI and T7 DNA ligase in a one-step digestion-ligation reaction to produce the CRISPR gRNA.
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bioRxiv - Plant Biology 2023Quote: ... and MEB3 cDNA were cloned into pAG416GAL-EGFP-ccdB (Addgene plasmid # 14315) vector via the LR reaction.
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bioRxiv - Plant Biology 2023Quote: ... HindIII-BamHI-cut CmYLCV was cloned into HindIII-BamHI-cut pnCas9-PBE vector from Addgene (Addgene plasmid #98164) to build pCmYLCV-nCas9-PBE ...
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bioRxiv - Plant Biology 2023Quote: ... HindIII-BamHI-cut CmYLCV was cloned into HindIII-BamHI-cut pnCas9-PBE vector from Addgene (Addgene plasmid #98164) to build pCmYLCV-nCas9-PBE ...
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bioRxiv - Plant Biology 2023Quote: ... All four Level 1 cassettes were assembled in a one-step reaction into the Level 2 acceptor plasmid (pAGM4723 Addgene #48015) as previously described (Dudley et al. ...
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bioRxiv - Plant Biology 2023Quote: ... and constitutive expression of SpCas9 (pEPQD1CB0001, Addgene #185625). All four Level 1 cassettes were assembled in a one-step reaction into the Level 2 acceptor plasmid (pAGM4723 Addgene #48015 ...
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bioRxiv - Plant Biology 2023Quote: ... expression cassettes were assembled by amplifying the sgRNA scaffold with an extended stem (Chen et al., 2013a) from plasmid pEPOR1CB0022 (Addgene #117537) and integrating the spacer sequence for each target as a 5’ extension of the forward primer ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting amplicons were assembled in Level 1 acceptors with an AtU626 promoter (pICSL90002, AtU6-26 Addgene#68261). The final construct was assembled by combining the two Level 1 sgRNA cassettes with cassettes for resistance to kanamycin pICSL11024 (Addgene#51144 ...
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bioRxiv - Plant Biology 2023Quote: Spacer sequences for selected targets were incorporated into pEPQD0KN0750 (Addgene #185627) by Golden Gate assembly into AarI sites as previously described (Dudley et al. ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana plants constitutively expressing Cas9 (Cas9 Benthe 193.22 T5 Homozygous; gratefully received from Dan Voytas) together with a strain containing pTRV1 (Addgene #148968) as previously described (Ellison et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The final construct was assembled by combining the two Level 1 sgRNA cassettes with cassettes for resistance to kanamycin pICSL11024 (Addgene#51144) and constitutive expression of SpCas9 (pEPQD1CB0001 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and inserted into pCAGGS from pCAGGS-PSD95-GCaMP2 (#18931, Addgene) vector for specific expression in β-cells ...
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bioRxiv - Systems Biology 2023Quote: Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene #84832) via BstXI-BlpI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... downstream of the hU6 promoter in a PX458 (Addgene #48138) Cas9 expression vector by golden-gate assembly with BbsI-HF (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... the backbone of pSH-EFIRES-P-AtAFB2-mCherry (Addgene #129716) was PCR-amplified to include both homology arms to AAVS1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... was cloned under ins2 promoter from pGL410_Ins2500 (#49058, Addgene) and inserted into pCAGGS from pCAGGS-PSD95-GCaMP2 (#18931 ...
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bioRxiv - Plant Biology 2023Quote: ... The module for selection was obtained from pHDE-35S-Cas9-mCherry-UBQ which was a gift from Yunde Zhao (Addgene plasmid # 78932; http://n2t.net/addgene:78932; RRID:Addgene_78932) (Gao et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The module for selection was obtained from pHDE-35S-Cas9-mCherry-UBQ which was a gift from Yunde Zhao (Addgene plasmid # 78932 ...
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bioRxiv - Systems Biology 2023Quote: ... We ordered pairs of complementary forward and reverse single stranded oligonucleotides (IDT) for each guide containing compatible overhang sites for insertion into a lentiCRISPRv2-blast (Addgene #83480) backbone ...
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bioRxiv - Systems Biology 2023Quote: ... The CRISPRi-v2 (5 sgRNA/TSS, Addgene: Cat#83969) sgRNA library was transduced into Jurkat cells at an MOI < 0.3 (BFP+ cell percentages were ∼30%) ...
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bioRxiv - Systems Biology 2023Quote: Jurkat cells expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells.
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bioRxiv - Systems Biology 2023Quote: ... donor DNA constructs were chemically synthesized and cloned into the pUC19 (IRF2BP2) or pAAV-MCS2 (MYB, RUNX1, RUNX2, GFI1 and SPI1) plasmids obtained from Addgene (Watertown, MA). The donors included 400-800 bp homology arms flanking the inserted DNA sequence encoding the FKBP12 degradation tag as well as mScarlet ...
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bioRxiv - Systems Biology 2023Quote: ... mCherry-P2A-Puro fusion was cloned into the BTV backbone (Addgene #84771). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... An all-in-one Cas9/gRNA expression construct by cloning an AAVS1 sgRNA sequence derived from pCas9-sgAAVS1-2 PX458 (Addgene #129727) downstream of the hU6 promoter in a PX458 (Addgene #48138 ...
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bioRxiv - Microbiology 2023Quote: ... and lentiviral particles expressing human TMPRSS2 (selection with 1 mg/ml G418). The TMPRSS2 (cat. 145843) and ACE2 (cat. 145839) lentiviral DNA clones (23) were purchased from Addgene and transfected into HEK293T cells together with pSPAX2 (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... first pCQ11:mNeongreen was constructed by restriction digest substitution of gfp in pCQ11:gfp with mNeongreen obtained from pLOM-S-mNeongreen-EC18153 (Julian Hibberd, Addgene plasmid # 137075; http://n2t.net/addgene:137075; RRID: Addgene_137075). pCQ11:isdF-mNeongreen was constructed via restriction digest ...
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bioRxiv - Microbiology 2023Quote: ... ORF3a (Addgene, cat.141383) (25 ng ...
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bioRxiv - Microbiology 2023Quote: ... and pBTK401 (Type 8) (Addgene_65109, Addgene_183127 ...
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bioRxiv - Microbiology 2023Quote: ... The pUCmini-iCAP-PHP.eB was a gift from Viviana Gradinaru (Addgene plasmid # 103005; http://n2t.net/addgene:103005 ; RRID:Addgene_103005).
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bioRxiv - Microbiology 2023Quote: ... and pBTK401 (Type 8) (Addgene_65109, Addgene_183127, Addgene_65179, Addgene_183126, Addgene_110593, Addgene_110597), and dsRNA target sequence (Type 3 ...