-
No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... Mouse anti-Nuclear Pore Complex Proteins antibody (Abcam; ab24609) was used at 1:500 and detected with goat anti-mouse IgG H&L (FITC ...
-
No products found
because this supplier's products are not listed.
Amy F Chen, et al.,
bioRxiv - Genomics 2021
Quote:
... nuclear pore complex antibody (Biolegend 902901), and GFP (Biolegend 338002) ...
-
No products found
because this supplier's products are not listed.
Ruhul Amin, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Reilly L. Allison, et al.,
bioRxiv - Neuroscience 2023
Quote:
... DAPI nuclear dye (Invitrogen) was used to label nuclei ...
-
No products found
because this supplier's products are not listed.
Jennifer M McCracken, et al.,
bioRxiv - Physiology 2023
Quote:
... The Avidin/Biotin complex (Vector Labs) was used to amplify the signal that was then detected with DAB (Vector Labs) ...
-
No products found
because this supplier's products are not listed.
Andrea Sacchetti, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The DAPI nuclear dye (D9542, Sigma-Aldrich) was used at 1 µg/ml to exclude dead cells ...
-
No products found
because this supplier's products are not listed.
Axelle Brulport, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
-
No products found
because this supplier's products are not listed.
Tanja Bhuiyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the total protein concentration in the nuclear and cytoplasmic extracts was measured using Protein Assay Dye Reagent Concentrate (Biorad, 5000006). 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek ...
-
No products found
because this supplier's products are not listed.
Charles Arber, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sections were incubated in avidin-biotin complex (ABC, Dako) for 30 minutes and the antigen-antibody reaction was visualized using di-aminobenzidine (DAB ...
-
No products found
because this supplier's products are not listed.
Ilya Chuykin, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Proteins were detected by the immunoblotting with goat anti-biotin-HRP antibodies (Cell Signaling) or goat anti-biotin antibody (Pierce) ...
-
No products found
because this supplier's products are not listed.
Xianhui Liu, et al.,
bioRxiv - Physiology 2024
Quote:
... Nuclear proteins were extracted using nuclear extraction buffer (NEB) (20mM HEPES pH7.9 ...
-
No products found
because this supplier's products are not listed.
Máté Kiss, et al.,
bioRxiv - Immunology 2020
Quote:
... or biotin-labeled antibodies (Jackson Immunoresearch) were applied ...
-
No products found
because this supplier's products are not listed.
Aisha Hamid, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
-
No products found
because this supplier's products are not listed.
Amrita Rai, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and Protein Complex suites (Qiagen). The sitting-drop vapor diffusion method was employed ...
-
No products found
because this supplier's products are not listed.
Junyan Lin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... mouse monoclonal anti-Nup93 (E-8) (Santa Cruz Biotechnology, sc-374399), rabbit monoclonal anti-Nup98 (C39A3 ...
-
No products found
because this supplier's products are not listed.
Youngseo Cheon, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... nuclear run-on reactions were performed with 25μM biotin-11-UTP (PerkinElmer, NEL543001EA), 25μM biotin-11-CTP (PerkinElmer ...
-
No products found
because this supplier's products are not listed.
Dennis Das Gupta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... followed by incubation with an anti FITC/streptavidin/biotin/magnetic bead complex (Miltenyi Biotec) and magnetic sorting using a microcentrifugation tube stand (Miltenyi Biotec).58 Sorting efficiency ...
-
No products found
because this supplier's products are not listed.
Florentina Negoita, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The protein-antibody complex on the membranes were visualized by Odyssey XF Imager (LI-COR Biotech, LLC, Germany) after incubation of the membrane with the enhanced chemiluminescence substrate (Millipore ...
-
No products found
because this supplier's products are not listed.
Jack H. Brelstaff, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Proteins were transferred onto 0.2 μm pore PVDF membranes (Merck). Nonspecific background was blocked in 5% w/v dry skimmed milk (Sigma ...
-
No products found
because this supplier's products are not listed.
Sk. Kayum Alam, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and BioTracker nuclear red dye (Sartorius). The plates were placed in IncuCyte for up to 96 hours with images taken every 24 hours.
-
No products found
because this supplier's products are not listed.
Benjamin Liffner, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibodies (anti-HA biotin conjugate 1:1000 (Roche), mouse anti-RAP148 1:500 ...
-
No products found
because this supplier's products are not listed.
Rana Lebdy, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Antibodies against the following proteins were used: Biotin (Bethyl A150-109 and Jackson Immunoresearch 200-002-211) ...
-
No products found
because this supplier's products are not listed.
Goutam Dey, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... nuclear protein lysates were incubated with 3µg anti-Myc antibody (Proteintech) or 3µg control antibody (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Dong Liu, et al.,
bioRxiv - Immunology 2024
Quote:
... and then incubated overnight at room temperature with fluorescently conjugated antibodies or DNA dyes (Nucspot ® Nuclear Stains 750/780, Biotium, #41038 ...
-
No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Biotin-Protein L was purchased from GenScript. BsiWI was purchased from New England Biolabs ...
-
No products found
because this supplier's products are not listed.
Parisa Yousefpour, et al.,
bioRxiv - Bioengineering 2024
Quote:
... or IgA-biotin-conjugated antibodies (Rockland), respectively ...
-
No products found
because this supplier's products are not listed.
Seong Ho Hong, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
-
No products found
because this supplier's products are not listed.
Mirca S. Saurty-Seerunghen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Transwells (8μm pores, Corning) were coated for 30min with Matrigel (0.2mg/mL culture media ...
-
No products found
because this supplier's products are not listed.
Monika Rakoczy-Trojanowska, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The protein-antibody complex was detected with the ECL Superbright system (Agrisera) and photographed using the ChemiDoc Imaging System (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Chenglong Xia, et al.,
bioRxiv - Biophysics 2024
Quote:
Nuclear-targeting Csm complex plasmid construction has been described previously12 (Addgene plasmid #195242). Cytoplasmic-targeting Csm complex plasmid was modified from this by removing the NLS sequences before each protein sequence ...
-
No products found
because this supplier's products are not listed.
Anthony Chifor, Jeongyoon Choi, Joongkyu Park,
bioRxiv - Neuroscience 2021
Quote:
... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
-
No products found
because this supplier's products are not listed.
Gali Maor, Ronald R. Dubreuil, Mel B. Feany,
bioRxiv - Neuroscience 2023
Quote:
... biotin-conjugated secondary antibodies (1:200, SouthernBiotech) and avidin-biotin-peroxidase complex (Vectastain Elite ...
-
No products found
because this supplier's products are not listed.
Helen O. Masson, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Proximal protein biotinylation occurs with treatment of H2O2 and tyramide-biotin (TSA Biotin System, Akoya Bioscience NEL700A001KT), resulting in tyramide-biotin radicalization and covalent deposition onto proximal proteins ...
-
No products found
because this supplier's products are not listed.
Henry J. Bailey, et al.,
bioRxiv - Biophysics 2024
Quote:
... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
-
No products found
because this supplier's products are not listed.
C.D. Kinz-Thompson, et al.,
bioRxiv - Biophysics 2022
Quote:
... Cleaved MSP protein was subsequently biotinylated using EZ-Link Maleimide-PEG11-Biotin (Promega) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... We verified the identity of the proteins in the complex by western blot using native antibody for Cbp1 and a mouse anti-his tag antibody (ABclonal AE003) for Yps3-6xhis.
-
No products found
because this supplier's products are not listed.
Mark R. MacRae, et al.,
bioRxiv - Biophysics 2022
Quote:
... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Takayuki Torisawa, et al.,
bioRxiv - Biophysics 2024
Quote:
Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
-
No products found
because this supplier's products are not listed.
Araceli Perez-Lopez, et al.,
bioRxiv - Immunology 2022
Quote:
... human anti-myeloperoxidase (MPO)-Biotin antibody (clone MPO421-8B2, Novus Biologicals), and APC/Cy7 streptavidin (BioLegend) ...
-
No products found
because this supplier's products are not listed.
Thadeu Estevam Moreira Maramaldo Costa, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Antibodies against human PCNA (proliferating cell nuclear antigen, rabbit) were purchased from Sino Biological (China). DNase I recombinant RNase free was purchased from Roche Life Sciences (Germany).
-
No products found
because this supplier's products are not listed.
Yuting Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 0.5 µL Tn5-Adapter complex (Diagenode) was added and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Michael Ferns, et al.,
bioRxiv - Neuroscience 2024
Quote:
... A dye-conjugated light chain-specific anti-rabbit secondary antibody (Jackson Labs) was used to detect bound Kv5.1 antibody ...
-
No products found
because this supplier's products are not listed.
Hirotatsu Imai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Antibodies against ribosomal proteins uL3 (GTX114725, GeneTex), L10a (A305-061A ...
-
No products found
because this supplier's products are not listed.
Dingding Mo,
bioRxiv - Neuroscience 2022
Quote:
... About 40 µg total protein of the supernatants were fractioned on 8–20% precast protein gels and transferred to 0.2 µm pore-size nitrocellulose membranes (66485, Pall). For monomer Aβ detection ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... antibodies in Protein LoBind tubes (Eppendorf). Thereafter ...
-
No products found
because this supplier's products are not listed.
Zaki Al-Yafeai, et al.,
bioRxiv - Cell Biology 2020
Quote:
... NF-κB nuclear translocation was visualized by Leica TCS SP5 confocal microscope using 63X (oil objective ...
-
No products found
because this supplier's products are not listed.
Arun Chhikara, et al.,
bioRxiv - Neuroscience 2024
Quote:
Primary antibodies and dyes used in this study were purchased from the following sources: Bassoon (Synaptic Systems, 141 004, 141021), Beta-actin (Synaptic Systems ...
-
No products found
because this supplier's products are not listed.
Rossana Azzoni, et al.,
bioRxiv - Immunology 2022
Quote:
... cells were stained in the dark for 20 minutes at 4°C with fluorochrome- or biotin-conjugated antibodies in PBS containing anti-CD16/CD32 (2.4G2; BioXcell) in the dark ...
-
No products found
because this supplier's products are not listed.
Xinyi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... but with different rabbit polyclonal antibodies from the Human Protein Atlas (HPA) purchased from Atlas Antibodies. On the next day ...
-
No products found
because this supplier's products are not listed.
Rebekah L. Rashford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... (3) Incubating the slices in primary antibody [1:500 Anti-Green Fluorescent Protein (Chicken) Antibody (Aves Labs); 1:150 SETD7 Mouse Monoclonal Antibody (OriGene) ...