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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The DAPI nuclear dye (D9542, Sigma-Aldrich) was used at 1 µg/ml to exclude dead cells ...
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bioRxiv - Developmental Biology 2022Quote: ... and Hoechst 33258 fluorescent nuclear dye (Sigma) in PBS/0.3% Triton X-100/10% BSA for 1h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... dyes or DBCO-biotin (Sigma-Aldrich) as the alkyne-azide cycloaddition ...
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bioRxiv - Neuroscience 2020Quote: ... and corresponding secondary antibodies as well as the nuclear dye Hoechst and fibrillar Aβ dye Thiazine red (ThR 2 μM, Sigma Aldrich). Full sections were analyzed by tile scan mode on a Leica SP5 II confocal microscope ...
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bioRxiv - Cell Biology 2023Quote: ... or H33342 nuclear dye (Sigma; B2261, 1 μg·mL−1) and ready for imaging ...
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bioRxiv - Microbiology 2021Quote: ... 2x protein inhibitor complex (Sigma) in PBS was added and protein levels were normalized by Bradford assay ...
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bioRxiv - Immunology 2024Quote: ... Nuclear DNA was labelled with DAPI dye (Sigma-Aldrich, MBD0015) at room temperature for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Counterstained was performed by the fluorescent nuclear dye Dapi (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent nuclear dye DAPI (1 μg/ml, #D9542, Sigma-Aldrich) was included with the secondary antibody solution ...
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent nuclear dye DAPI (1 μg/ml, #D9542, Sigma-Aldrich) was included with the secondary antibody solution ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Genomics 2020Quote: ... of GAPDH (cytoplasmic protein) and Histone H3 (nuclear protein) using rabbit anti-GAPDH antibody (1:5000, Sigma) and rabbit anti-histone H3 antibody (1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
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bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... Anti Neuronal Nuclear antibody (Millipore, NeuN ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed thrice with 0.1% Triton-X in PBS followed by incubation with secondary antibodies and 1µg/mL Hoechst (nuclear dye) (Sigma-Aldrich) for 2 hr at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... The following nuclear dyes were used: DAPI (1:1000, Sigma-Aldrich D9542); To-Pro-3 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were counterstained with the fluorescent nuclear dye DAPI (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... the sections were counterstained with the fluorescent nuclear dye DAPI (Sigma-Aldrich). For Nissl staining ...
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bioRxiv - Cancer Biology 2022Quote: ... were used as well as a fluorescent nuclear dye (Sigma, Hoechst 33258). Slides were finally mounted with Dako Fluorescent Mounting Medium ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Biophysics 2023Quote: ... The complex was isolated from the nuclear fraction using anti-FLAG M2 beads from Sigma aldrich. The nuclear fraction was mixed with washed FLAG M2 beads and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with 0.1% Triton-X in PBS followed by incubation with secondary antibodies and DAPI (nuclear dye) (Sigma, USA) for 2 hr at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... then the antibody-protein complex was captured with EZview A/G beads (Millipore Sigma, Cat #: E3403). Beads were pelleted and washed with lysis buffer 3x ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 10 minutes of incubation with the nuclear dye 1X Hoechst (Sigma, 33258) (Vannocci et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... Mediator complex in the nuclear extract was affinity-purified by anti-FLAG M2 Affinity gel (Sigma-Aldrich, A2220) and washed extensively by BC100 buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... followed by avidin-biotin complex and developed in solution containing 0.025% 3,3’-diaminobenzidine tetrahydrochloride (Sigma-Aldrich), 10 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and counter-stained with DAPI nuclear dye (0.5 µg/ml in PBS; 10 min; Sigma). Cells incubated with secondary antibodies alone served as negative controls ...
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bioRxiv - Neuroscience 2024Quote: ... Counterstaining was performed using the fluorescent nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (Sigma). In situ hybridization was performed on 60 μm vibratome sections using digoxigenin-labelled antisense probe for Rorα ...
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bioRxiv - Bioengineering 2024Quote: ... secondary antibodies and nuclear counterstain (DAPI, Sigma-Aldrich) were added and incubated at RT in dark for 45 min (see Table 2 for details of primary and secondary antibodies and concentrations) ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: ... The extraction of nuclear proteins has been performed using the CelLytic™ NuCLEAR™ Extraction kit (Sigma-Aldrich) complemented with PIC ...
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bioRxiv - Plant Biology 2023Quote: ... and Peroxidase anti-Peroxidase Soluble Complex antibody (P1291, Sigma) were also used.
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bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2022Quote: ... Argonaute proteins/small RNA complex were extracted from 50,000 synchronized worms and immunoprecipitated with anti-flag antibody (Sigma Aldrich) or GFP nanobody (Thermo) ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were detected with antibodies after western blotting: ABCE1-TAP with peroxidase anti-peroxidase (PAP) complex (Sigma-Aldrich) at 1:2,000 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Neuroscience 2022Quote: ... Vibratome sections (thickness: 80 μm) were then counterstained with the fluorescent nuclear dye DAPI (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... the streptavidin aptamer and bound complex were eluted using 50 µl 10 mM Biotin (pH 7.2) (Sigma, B4501) suspended in the 1X Aptamer buffer and stored at −20°C ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse Cy3 anti-biotin antibody (#C5585 - Sigma) at 1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... nuclear and cytoplasmic proteins were reduced with 5 mM DTT (Sigma) for 1 h at room temperature and alkylated with 20 mM iodoacetamide (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...