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Citations for Leica :
1 - 50 of 322 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... NF-κB nuclear translocation was visualized by Leica TCS SP5 confocal microscope using 63X (oil objective ...
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bioRxiv - Cell Biology 2023Quote: ... Dexamethasone-induced nuclear import of XRGG-GFP was recorded by Leica CSU-W1 spinning disk (63X objective ...
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bioRxiv - Physiology 2024Quote: Nuclear immunoreactivity was assessed with a binocular microscope (DMLB®, Leica) with a digital camera (DC480 ...
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bioRxiv - Cancer Biology 2024Quote: ... and analyzed using the Aperio Nuclear Algorithm v9 (Leica Biosystems Ltd). Ovarian tumor epithelial cells were distinguished from stromal cells by the shape and size of their nuclei ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with Orange G dye (Leica)) and separated by native gel electrophoresis in TG buffer using 4–20% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Bioengineering 2023Quote: ... A biotin-free polymeric IHC detection system (Leica DS9800) consisting of HRP conjugated Gt anti-Rb IgG secondary antibody was applied for 25 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated with antibodies to p53 protein (CM5) (Leica Biosystems), EpCAM (CD326 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% (w/v) Orange G dye (Leica), in deionized water ...
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bioRxiv - Neuroscience 2020Quote: ... TBR1 for immature neurons) and nuclear counterstaining (DAPI) in immunofluorescence followed by Leica SP5 confocal microscopic analysis ...
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bioRxiv - Genetics 2021Quote: ... Slides were incubated with primary antibody against p53 protein (CM5; Leica Biosystems) and then with secondary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Aperio IHC Nuclear Image analysis algorithm in ImageScope v.12.3.2.8013 (Leica Microsystems). Cell counting was performed in 1 mm2 tumor-rich areas.
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bioRxiv - Biochemistry 2024Quote: ... 10 μg the mouse p53 polyclonal antibody CM5 was used from Leica (#P53-PROTEIN-CM5) or control mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: ... Chlorophyll fluorescence and any nuclear GFP signal were acquired simultaneously using filters (480/40, dichroic 505 (Leica) or dichroic 510 (Nikon) ...
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bioRxiv - Genomics 2024Quote: ... Individual nuclear profiles (NPs) were isolated by laser microdissection using a laser microdissection microscope (Leica Microsystems, LMD7000). Cut NPs were collected in a PCR Cap Strip filled with opaque adhesive material (Carl Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... The Channel Dye Separation module within the LAS X 3.5.5.19976 (Leica) was then used to correct for any residual spillover ...
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bioRxiv - Neuroscience 2022Quote: ... Imaging of nuclear pCREB/CREB immunoreactivities in cryosections was performed using a Leica TCS SP5 system (Leica-Microsystems). Images were acquired sequentially with a HCX ApoL20x/1.0 water objective ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as DAPI (for nuclear counterstaining) were conducted on a Leica Bond Rx automated autostainer (Leica Biosystems). The panel of primary antibody/fluorophore pairs was applied to spectrally co-localize them in the same cellular compartment (AQP1/Opal 480 ...
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bioRxiv - Genomics 2024Quote: ... DAPI was applied for 5 min for nuclear counterstaining before visualization using confocal microscopy (Leica TCS SP5 MP). PLA was performed usin the DuolinkII fluorescence system (Olink Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... staining was quantified using the Aperio Nuclear Algorithm software after scanning the images using Aperio Imagescope (Leica Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... as well as DAPI (for nuclear counterstaining) were conducted on a Leica Bond Rx automated autostainer (Leica Biosystems). The panel of primary antibody/fluorophore pairs was applied to spectrally co-localize them in the same cellular compartment (p-HDAC4Ser632/Opal 480 ...
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bioRxiv - Neuroscience 2022Quote: For quantitation of nuclear staining intensity (pCREB or CREB) somatic regions were sequentially scanned using the 63x objective (Leica) in both ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were mounted in anti-fade mounting medium supplemented with DAPI for nuclear staining and imaged by confocal microscopy (Leica SP5 X MP ...
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bioRxiv - Microbiology 2021Quote: ... ToPro3 was used for nuclear staining and a super-resolution Leica TCS SP8 STED 3X microscope (Leica Microsystems GmbH, Germany) equipped with 592 and 660nm depletion lasers was used to image and collect data ...
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bioRxiv - Neuroscience 2024Quote: ... the entire CG-SMG ganglia complex was whole-mount and z-stack imaged to visualize all projecting neurons via confocal microscopy (Leica, TCS SP8). By digitally drawing vertical lines from both the left and right sides towards the center ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry (IHC) for the Neuronal Nuclear Antigen (NeuN) was performed using an automated immunostainer (Bond III, Leica or Infinity i6000, Biogenex). Slides were rehydrated ...
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bioRxiv - Neuroscience 2022Quote: ... cryopreserved and cryosectioned organoids (12-15 μm sections) were imaged following nuclear staining using Hoechst using a Leica DMI6000B microscope (Leica Microsystems) with an air 4x objective driven by Las-AF software ...
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bioRxiv - Biochemistry 2023Quote: ... Ki-67 nuclear staining have been automatically detected in the epithelium lining cortical cysts by Aperio Image analysis software (Leica Biosystems). Positive nuclei have been manually counted and normalized on the total number of nuclei in cyst epithelium.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Immunology 2024Quote: ... which was created using the Leica LAS-AF Channel Dye Separation module (Leica Microsystems) and tissue sections stained with a single antibody–fluorophore ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were mounted in anti-fade mounting medium supplemented with DAPI for nuclear staining and imaged by confocal microscopy (Leica SP5 X MP, 63× oil lens, Leica Camera, Wetzlar, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... At least three randomly-selected and non-overlapping fields on each core of the tissue microarray were examined using nuclear algorithm of the Aperio ImageScope software (Leica Biosystems, Buffalo Grove, IL). Data is expressed as H-score that is based on intensity (0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were scanned using the Aperio AT2 digital scanning system and HAF nuclear staining intensity quantitation was performed using Aperio digital imaging software (Leica Biosystems, Buffalo Grove IL) and statistical analysis performed using unpaired Student’s T-tests in GraphPad Prism software 10.10.2 (GraphPad Software ...
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bioRxiv - Immunology 2024Quote: ... Next fluorophore spillover was calculated and corrected using the Channel Dye Separation module in LAS X (Leica). Cell surfaces were created using Nucspot 750/780 nuclear staining using the Imaris surface creation module ...
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bioRxiv - Immunology 2024Quote: ... and by creating a compensation matrix via the Leica LAS-AF Channel Dye Separation module (Leica Microsystems) per user’s manual ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Neuroscience 2021Quote: ... and the spread of dye was monitored with a microscope camera (MC190 HD, Leica Microsystems GmbH, Wetzlar, Germany).
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bioRxiv - Plant Biology 2021Quote: ... and fluorescent dye signals conjugated to EdU were observed under fluorescent microscope (DM4000; Leica Microsystems GmbH, Wetzlar, Germany).
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and drainage of the dye to the DCLNs was visualized under a S6D stereomicroscope (Leica). For quantitative assessment ...
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bioRxiv - Immunology 2022Quote: RAW 264.7 cells stained by immunofluorescence and/or LysoTracker dye were imaged with a TCS SP8 confocal microscope (Leica) using a 63x oil immersion objective (NA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Incorporation of FM1–43 dye into fibers was immediately recorded by a spectral LEICA TCS SP8 confocal microscope (Leica). Tile images were acquired under LASX Software (Leica ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The collection of the resulting emission was system optimized to maximize yield and minimize crosstalk (Leica Dye Finder, LASX software; Leica).
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bioRxiv - Genetics 2023Quote: ... Excess dye was washed off and animals were imaged via fluorescence stereoscope imaging for Nile Red using a M250FA stereoscope (Leica) or brightfield/colorimetric imaging for Oil Red O using a Revolve microscope (Echo) ...
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bioRxiv - Neuroscience 2023Quote: ... The emitted light’s filtering and collecting were adjusted to increase yield and reduce cross-talk (Leica Dye 164 Finer, LasX software, Leica). A hybrid detector with 100% gain was used to find GCaMP and a photomultiplier tube was used to find mCherry (500-600V gain) ...
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bioRxiv - Neuroscience 2023Quote: ... The emitted light’s filtering and collecting were adjusted to increase yield and reduce cross-talk (Leica Dye 164 Finer, LasX software, Leica). A hybrid detector with 100% gain was used to find GCaMP3 and a photomultiplier tube was used to find mCherry (500-600V gain) ...
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bioRxiv - Microbiology 2023Quote: ... or FM464 (5 µg/mL) dyes for 30 min and imaged by phase-contrast and fluorescence using an inverted DeltaVision Elite Microscope (Leica) equipped with an UPLFLN100XO2/PH3/1.30 objective (Olympus) ...
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bioRxiv - Developmental Biology 2024Quote: ... or 1 mg/mL SCRI Renaissance 2200 dye (SR2200) (Renaissance Chemicals) and imaged in a Leica SP8 laser-scanning confocal microscope (Leica) using a hybrid detector counting mode and the following settings ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies were diluted in Antibody Diluent (Leica) as indicated ...
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bioRxiv - Neuroscience 2020Quote: ... The collection of the resulting emission was system optimized to maximize yield and minimize crosstalk (Leica Dye Finder, LASX software; Leica).