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Citations for Takara Bio :
1 - 50 of 713 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was purified using TALON beads (Clontech) after nucleic acid precipitation using 0.05% of PolyEthylImine (PEI ...
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bioRxiv - Microbiology 2023Quote: ... Dye Plus (Takara), and primers (46 ...
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bioRxiv - Systems Biology 2020Quote: Human mitochondrial to nuclear DNA ratio kit (Takara) was used to assess mitochondrial DNA content ...
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bioRxiv - Cell Biology 2023Quote: ... crossed with WT BDF1 males at E0.5 and electroporated with RNP complex of Mavs targeting sgRNA (100 ng μL-1) and Cas9 protein (Takara Bio; 500 ng μL-1) using NEPA21 electroporator ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies and dyes were used at following dilutions: rabbit-α-dsRed (1:500, Takara, #632496; RRID:AB_10013483), α-HRP conjugated with Alexa Fluor-488 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... Red Fluorescent Protein Antibody (DsRed, 1:500, Clontech). The brain sections were incubated with antibodies in blocking solution at 4°C temperature overnight ...
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bioRxiv - Biophysics 2020Quote: ... The complex was then purified by binding on Talon resin (Clontech) and eluted in 150 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 1x ROX reference dye (Takara), 300 nM of each forward and reverse primer (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... SYBR dyes (Takara, Shiga, Japan) were combined into a 12ul system for cDNA amplification and signal collection ...
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bioRxiv - Plant Biology 2020Quote: ... the YFP protein was detected using the GFP antibody (Clontech) at 1/5,000th and the UGPase antibody (Agrisera ...
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bioRxiv - Neuroscience 2023Quote: ... Green Fluorescent Protein (GFP) antibody (JL-8, 1:500, Clontech), Red Fluorescent Protein Antibody (DsRed ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4 μL ROX Reference Dye (50×) (TaKaRa), 0.8 μL of each primer (10 μM) ...
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bioRxiv - Plant Biology 2020Quote: ... YFP-CESA6 protein was detected using anti-GFP antibody (Takara, catalog # 632381) and SEC12 was detected using anti-SEC12 antibody (Bar-Peled and Raikhel ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial DNA and nuclear DNA and were extracted using NucleoSpin® DNA RapidLyse (Takara). Mouse NADH dehydrogenase (mt-Nd1 ...
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bioRxiv - Microbiology 2022Quote: ... 0.4 μL of ROX reference dye (50×; TaKaRa), 0.8 μM of each primer ...
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bioRxiv - Plant Biology 2021Quote: ... The gRNA - dCas9 complex was amplified (primers in Supplemental Table 1) and In-Fusion cloned (Clontech) into a SacI digested pMJS064 (described above ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Biochemistry 2021Quote: ... 0.4 μL ROX Reference Dye (50×) (Takara, Japan, RR420A), 1.0 μL DNA template and 0.8 μL each of the primers (final concentration was 0.4 μM) ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Complex formation was induced in these cells by treating with AP21967 (A/C ligand heterodimerizer; Takara Bio). MDA-MB-231-iDimerize-c-Met-β1/luc cells were created by lentiviral transduction of MDA-MB-231-iDimerize-c-Met-β1 cells and subsequent confirmation of bioluminescence ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear and mitochondrial DNA were isolated using a NucleoSpin Tissue gDNA isolation kit (Takara Bio 740952) per manufacturer’s instructions and nuclear and mitochondrial DNA was quantified via qPCR assessment of 28S and COX1 levels ...
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bioRxiv - Neuroscience 2020Quote: ... with double-stranded DNA dye SYBR Green (RR091A, Takara, Japan). Complementary DNA from various cell samples or hippocampal tissue samples were amplified with specific primers (Lox ...
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bioRxiv - Genomics 2021Quote: ... 12.5 μl Premix Taq (TaKaRa TaqTM Version 2.0 plus dye), Primer_1 (2×0.1 μl) ...
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bioRxiv - Cell Biology 2023Quote: ... put on ice to form RNA/primer complex and mixed with 4.5 µL of RT solution {0.5 µL of SMARTScribe RTase (cat. #639536, Takara), 2 µL of 5x first strand buffer (supplied with RTase) ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear RNA was processed into cDNA libraries using the SMART-Seq v4 Ultra Low Input kit (Takara) using 19 PCR cycles ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed with SYBR green fluorescent dye (Takara, Japan) using a Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... The collected media containing virus was filtered through 0.45μm pore filter and mixed with Lenti-X concentrator (Clontech) as indicated by manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The viral supernatants were filtered with 0.45 µm pore size and then concentrated using the Lenti-X concentrator (Takara) following manufacturer’s instruction.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was filtered through an 0.8-μm pore-size filter and subsequently loaded onto a TALON Metal Affinity Resin (Clontech) equilibrated with the lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then stained using primary antibodies against the reporter proteins mCherry (Clontech 632543 RRID: AB_2307319, 1:250) and against the panneuronal marker NeuN (millipore MAB377 RRID ...
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bioRxiv - Plant Biology 2020Quote: ... GFP-TSN-interacting proteins and native TSN were detected by mouse α -GFP (monoclonal antibody JL-8; Clontech) and rabbit α-TSN antibodies at final dilutions of 1:1,000 and 1:5,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The expression of Dunk protein was confirmed by Western blot using c-Myc Monoclonal Antibody (Takara, Cat# 631206). Then ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA, 7246).
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bioRxiv - Biochemistry 2020Quote: ... Full-length EGFP fused N-terminally to a nuclear localization signal (NLS) and C-terminally to a Myc-tag was expressed from pCMV (Clontech).
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bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries of rRNA-depleted nuclear RNA or polyA RNA were prepared with SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: A mix of 4 μg of pcDNA3.1 plasmid containing the mouse Wnt10b cDNA (provided by Dr Bernardi, INRA, Montpellier) and 1 μg of pAcGFP-Nuc that encodes a nuclear GFP (Clontech) in 0.9% NaCl was electrotransfected directly into TA muscles of anesthetized ob/+ and ob/ob mice according to the protocol described by Grifone et al.[29] ...
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bioRxiv - Plant Biology 2023Quote: ... were PCR-amplified and fused to a nuclear localization signal (NLS) in pENTR vectors using In-Fusion HD Cloning Plus (Clontech). See Table S5 for primer details ...
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bioRxiv - Molecular Biology 2023Quote: ... and those targeting the Alu repeat sequence in the nuclear genome (5′-CTTGCAGTGAGCCGAGATT-3′ and 5′- GAGACGGAGTCTCGCTCTGTC-3′) (75) with TB Green Premix Ex Taq II (TaKaRa) on Thermal Cycler Dice Real Time System II (TaKaRa) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The SYBR® fluorescent dye (TB Green® Premix Ex Taq™, Takara) was employed for reporting the amplification of cDNA in the Applied biosystem 7500 Fast Real-Time PCR system with specific primer pairs for DFR gene (Supplementary Table 2) ...
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bioRxiv - Cancer Biology 2024Quote: ... media containing the virus were harvested and filtered through a 0.22 mm pore cellulose acetate filter and then concentrated with the Lenti-X Concentrator (Takara Bio) before quantitative characterization with the Lenti-X p24 Rapid Titer Kit (Takara Bio) ...
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bioRxiv - Plant Biology 2021Quote: ... we used the nuclear system Y2H mating standard operating procedure method as described in the manufacturer’s instructions (Clontech Laboratories, Inc., USA) to screen the protein that interacts with TiAP1 and further verified the obtained interaction protein ...
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bioRxiv - Plant Biology 2021Quote: ... NPR1-GFP proteins were detected by reacting the blots with the mouse monoclonal anti-GFP monoclonal antibody (Clontech, USA) and horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Plant Biology 2021Quote: The change in fluorescence of SYBR Green I dye (SYBR Premix Ex Taq, TaKaRa) in every cycle was monitored by the realplex system software ...