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Citations for NanoTemper :
1 - 50 of 214 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... one of the proteins was labeled with the fluorescent dye NT-647-NHS (NanoTemper Technologies) via amine conjugation ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by labelling with 30uM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at RT for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... followed by labeling with 30 μM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at Room Temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Molecular Biology 2022Quote: SiaQM in amphipol was labelled at a ratio of 0.8 dye / 1 protein following the protocol of the RED-NHS 2nd generation labelling kit (NanoTemper). Labelled SiaQM was held constant at 20 nM and SiaP was titrated at 16 concentrations starting at 2,900 μM and serially diluting 2-fold to 90 nM ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PDZ domains were covalently labelled using a fluorescent dye reactive on amine following the manufacture’s protocol (Protein Labelling Kit RED-NHS, Nanotemper). A serial dilution of the WT and the P71L mutant of the E protein PBM peptide was prepared in the buffer containing PBS tween 20 0.05% ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µL of 100 nM solution of his-tagged protein was labeled with RED-tris-NTA 2nd Generation dye (Monolith HIS-tag labeling Kit, NanoTemper). Protein-protein complexes were obtained by mixing dye-labeled IFIT protein (final concentration 50 nM ...
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bioRxiv - Biochemistry 2024Quote: ... RED-tris-NTA dye (NanoTemper), and 0.05% Tween 20 in CPD and incubated for 30 min at 20°C ...
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... 90 µl of 200 nM protein mixed 1:1 with 100 nM RED-tris-NTA 2nd generation dye (MO-L018, Nanotemper Technologies) and incubated at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... fluorescent dye (Cat no. L008, NanoTemper Technologies) and applied at the final concentration of 50 nM in His-SPN SEC buffer having 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... and labeled with RED-NHS dye (NanoTemper). PTX was prepared in 25 mM HEPES (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... AtExo70B1 and their complex were loaded in nanoDSF grade standard capillaries (NanoTemper Technologies GmbH, München, Germany) and exposed at thermal stress from 20 ⍰C to 95 ⍰C by thermal ramping rate of 1 aC/min ...
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bioRxiv - Plant Biology 2023Quote: The fluorescent dye NT-647 (MO-L001, NanoTemper Technologies) was used to label effector proteins GST or BSA ...
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bioRxiv - Molecular Biology 2024Quote: Complex thermostability was assayed by intrinsic fluorescence recorded using a Prometheus NT.48 nano-DSF instrument (NanoTemper technologies), essentially as previously described 55 ...
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bioRxiv - Biochemistry 2023Quote: Purified MEMO1 was labeled with Red-NHS dye (NanoTemper Technologies GmbH) and diluted in 10 mM MES ...
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bioRxiv - Plant Biology 2022Quote: ... The RED-NHS 2nd generation amine reactive dye (MO-L011, NanoTemper Technologies) was used to label the purified SIRK1-ECD (2 µM ...
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bioRxiv - Biochemistry 2024Quote: ... The branaplam titration was then mixed 1:1 with the pre-formed U1 snRNP complex and incubated at room temperature for 15 min before loading Monolith LabelFree Premium Capillaries (MO-Z025; NanoTemper Tech). Capillaries were analyzed by a Monolith X red-continuous instrument (NanoTemper Tech ...
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bioRxiv - Biophysics 2020Quote: ... Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies) in PBS-T (10 mM phosphate ...
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bioRxiv - Molecular Biology 2021Quote: Jagged1C2-EGF3,HA in SEC buffer was labelled with NT-547 dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... were labeled with the amine-reactive dye NT-647 Red-NHS (NanoTemper, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... purified PurTCp was labeled with RED fluorescent dye NT-647 (RED-tris-NTA; NanoTemper Technologies) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... GppNHp-loaded Rab21 (18) was labeled using amine-reactive NT-650-NHS fluorescent dye (Nanotemper) using 1:2 protein:dye concentration ratio ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper). A 1:1 serial dilution of unlabelled zGFRα1D1-3-zGDNFmat ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
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bioRxiv - Microbiology 2024Quote: The protein-DNA and protein-protein interaction were assayed by microscale thermophoresis (MST) using Monolith NT.115 (NanoTemper Technologies, Germany) as described earlier (Su et al ...
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bioRxiv - Plant Biology 2024Quote: ... NIT1 WT and AIM2 mutant were labeled with RED-NHS 2nd Generation dye (NanoTemper Technologies, München, Germany), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The purified recombinant His-tagged PfSir2A was labeled by the RED-tris-NTA 2nd generation dye (NanoTemper Technologies). The protein concentration was adjusted to 200 nM in PBS-T buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Target proteins were fluorescently labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). Labeling reaction was performed following the manufacturer’s instructions with 10 μM protein ...
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...