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Citations for Qiagen :
1 - 50 of 863 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and Protein Complex suites (Qiagen). The sitting-drop vapor diffusion method was employed ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: Specific enrichment of nuclear proteins from astrocytes and neurons (NS, SD, 3 biological replicates) was performed using a Qproteome Nuclear Protein Kit (QIAGEN, Cat # 37582) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Initial screens were performed using Nucleix and Protein Complex suites (Qiagen) in a sitting-drop setup ...
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bioRxiv - Genetics 2024Quote: ... The final protein complex was obtained by affinity purification on NiNTA agarose (Qiagen) via a 10x-His-tag on the C-terminus of MMS22L ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Biophysics 2021Quote: The nsp10:16 protein complex was supplemented by 1 mM sinefungin Crystals of the nsp10:nsp16 protein complex grew in three days in commercial JCSG I-IV screens (Qiagen). They were cryo-protected in mother liquor supplemented with 20% glycerol and flash frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Immunology 2021Quote: ... Crystals of SB16-RBD and SB45-RBD complexes and Sb16 alone were observed within one week using Protein Complex (Qiagen) and Wizard Classic 4 (Rigaku) ...
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bioRxiv - Cell Biology 2020Quote: The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Biophysics 2023Quote: ... 20 nM biotin-NTA (QIAGEN) was charged with 50 nM Ni2+ and incubated on the slide for 10 min before rinsing away the unbound excess biotin-NTA and Ni2+ with PBS (this step was omitted for the labeled DNA samples) ...
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bioRxiv - Genomics 2022Quote: ... The immunoprecipitated complex was treated with RNaseA (QIAGEN, 19101), and reverse-crosslinked overnight at 68°C ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was purified by Ni-NTA Superflow (Qiagen) and chitin bead (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... the purified complex was applied to Ni-NTA agarose (Qiagen) and rotated 60’ at 4° C ...
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bioRxiv - Neuroscience 2024Quote: ... then nuclear lysates were stored in Buffer RLT (Qiagen 79216) at -80C ...
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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bioRxiv - Genomics 2024Quote: ... and nuclear RNA were done using QIAseq FastSelect rRNA HMR (Qiagen) and UltraII Directional RNA Library Prep (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... the complex was purified by nickel affinity chromatography (Ni-NTA resin, Qiagen) and eluted with elution buffer (40mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: PaPcc1-PaPcc2 complex was purified by affinity chromatography on NiNTA column (Qiagen) at room temperature followed by size exclusion chromatography as described above except that the lysis buffer contained 300 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex was immobilized on 20 µL of Ni-NTA agarose beads (Qiagen) with occasional pipetting ...
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bioRxiv - Biophysics 2024Quote: ... The complex was initially screened by the following commercial crystallization screens: Nucleix (Qiagen) and Wizard I&II (Rigaku reagents) ...
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bioRxiv - Biochemistry 2024Quote: ... The 6×His-tag-FACT complex was captured with Ni-NTA beads (Qiagen) while rotating at 4 ℃ for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The complex was then incubated for 30 min with the Ni-NTA beads (Qiagen) at 4°C to capture the cleaved off tag and the protease ...
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bioRxiv - Neuroscience 2024Quote: Nuclear RNA was extracted from sorted nuclei (RNeasy Micro Kit, Qiagen ref 74004) and quantified using Tapestation 4150 (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclear RNA was extracted from sorted nuclei (RNeasy Micro Kit, Qiagen ref 74004) and quantified using Tapestation4150 (Agilent) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Immunology 2021Quote: ... Each complex was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) and ProPlex screen (Molecular Dimensions ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA and short RNAs purified from the SPARDA complex were processed with RQ DNAse (Qiagen) following the manufacturer’s protocol and subjected to phenol-chloroform extraction ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA from cytosolic and nuclear fraction were extracted using the RNeasy Mini Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: Nuclear or cytoplasmic RNA were separately extracted in accordance with a protocol provided from QIAGEN with slight modification (https://www.qiagen.com/us/resources/download.aspx?id=1f8ed6e7-2423-4cff-8013-0eb89ead5cb0&lang=en&ver=1 ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Cell Biology 2020Quote: ... RNA in either the nuclear pellets or cytoplasmic fractions was extracted with RNeasy Plus kit (Qiagen). Total RNA extracted per fraction was quantified and used to calculate the percentage of mRNA localization for the RT–qPCR quantification ...
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bioRxiv - Cell Biology 2022Quote: siRNA duplex for IPO9 was obtained from Ambion (id# S31299) and siRNA for the nuclear actin exporter XPO6 was obtained from Qiagen (id# SI00764099). All siRNA were transfected or co-transfected with emerin plasmids at 25 nM using X-tremeGENE HP (Roche) ...
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bioRxiv - Pathology 2022Quote: Total DNA containing nuclear DNA and mtDNA were isolated from LB using the DNeasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was modified by a custom-added biotin residue at its 5’-end (Qiagen). Hybrids of the CoV-2 RNAs with the probe were detected with a goat anti-biotin antibody conjugated to 10 nm gold particles (BBI international).
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bioRxiv - Microbiology 2020Quote: ... 5 and 0.22 μm pore-size filters were processed using the DNeasy PowerWater Kit (QIAGEN, Hilden, Germany) following the manufacture instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The complex (12 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Microbiology 2020Quote: ... The complex (7.5 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase inhibitors) and RNA-IKKα complex was pulled down by Ni2+-NTA Agarose Beads (Qiagen cat. no. 30230) or IKKα antibody ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Genomics 2021Quote: ... Nuclear DNA was extracted individually from 10 mg of tissue using the DNeasy Plant Mini Kit (QIAGEN, DE) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear RNA of NeuN+ and NeuN-nuclei was isolated by using the RNeasy® FFPE Kit (Qiagen, 73504) according to manufacturer’s protocol ...