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Citations for SouthernBiotech :
1 - 50 of 568 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... biotin-conjugated secondary antibodies (1:200, SouthernBiotech) and avidin-biotin-peroxidase complex (Vectastain Elite ...
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bioRxiv - Physiology 2021Quote: ... A goat anti-mouse IgG- biotin antibody (Southern Biotech) was diluted 1:5000 in reagent diluent and added to all wells ...
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bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
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bioRxiv - Immunology 2020Quote: ... anti-IgG1-biotin (Southern Biotech) and anti-IgG2a-PE ...
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bioRxiv - Cell Biology 2022Quote: ... DAPI was used for nuclear staining (Southern Biotech).
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bioRxiv - Microbiology 2022Quote: ... Slides were exposed to biotin labelled goat Fab anti-human IgG secondary antibody (Southern Biotech, Birmingham, AL) at a 1:2500 dilution for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the plates were incubated with serially diluted serum samples and then with biotin-conjugated detection antibodies (Southern Biotech) followed by streptavidin-conjugated HRP (Jackson Immunoresearch) ...
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bioRxiv - Immunology 2024Quote: ... biotin rat anti-mouse IgG1 (Southern Biotech), or biotin rat anti-mouse IgG2b (Southern Biotech) ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2024Quote: ... or biotin rat anti-mouse IgG2b (Southern Biotech). Total IgE was detected with biotin rat anti mouse IgE (Southern Biotech) ...
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bioRxiv - Physiology 2021Quote: ... and nuclear counterstain with DAPI using Fluoromount-G (0100-01, Southern Biotech). Slides were imaged using the PerkinElmer Vectra (Vectra 3.0.3 ...
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bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse IgG and IgG1 conjugated to biotin (Southern Biotech), High Sensitivity Streptavidin-HRP (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-mouse IgA- biotin (Southern Biotech, Cat. #1040-08) and then streptavidin-HRP (Vector laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-mouse IgA-biotin (Catalog #1040-80, Southern Biotech). After a 1h incubation at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... We applied nuclear DAPI counterstain and added Fluoromount-G (0100-01, SouthernBiotech, Birmingham, AL) before all slides were cover slipped for imaging (n-6-7/each treatment and sex).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were mounted with DAPI Fluoromount-G media with DAPI nuclear stain (Southern Biotech). Slides were viewed with Olympus microscopy.
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bioRxiv - Microbiology 2023Quote: ... IgA and IgG were detected by goat α-Human Fc IgA-Biotin conjugate and goat α-Human Fc IgG-Biotin conjugate (Southern Biotech, Birmingham, AL, USA) diluted 1:20,000 in PBS with 1% BSA and incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Rat anti-mouse IgG1-Biotin (SB77E) and Streptaviding-HRP (Southern Biotech) were used as detection and secondary antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100μL IgG-biotin HU adsorbed (Southern Biotech) for 2hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-mouse IgA biotin (Southern Biotech, 1: 5,000; AL, USA). Streptavidin-HRP (Vector Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08) diluted in PBS-BSA 1 % (100 μL/well) ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-mouse Alexa Fluor 488 dye conjugate (SouthernBiotech). Every hour ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 hr at RT ...
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bioRxiv - Immunology 2024Quote: ... Total IgE was detected with biotin rat anti mouse IgE (Southern Biotech). After washing 3 times in PBS/0.05% Tween ...
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bioRxiv - Immunology 2022Quote: ... for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech, #0100-01). Immunofluorescence staining with anti-LAMP2 antibody (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... a goat anti-mouse IgG biotin primary Ab (SouthernBiotech, Cat no. 1030-08) at (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated with hPD-L1-His protein and anti-PD-L1 antibody and anti-mouse IgG specific HRP conjugated secondary antibodies (SouthernBiotech, Birmingham, AL, USA) were added ...
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bioRxiv - Developmental Biology 2020Quote: ... was applied to the slides for nuclear staining prior to mounting in Fluoromount-G (Southern Biotech, 0100-01) with 34 x 60 mm coverslips (VWR ...
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bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
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bioRxiv - Neuroscience 2022Quote: ... was used for nuclear staining in brain slices and DAPI Fluoromount-G (catalog#0100-20, Southern Biotech, Birmingham, AL, USA) was used for cultured cells.
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
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bioRxiv - Systems Biology 2021Quote: ... DAPI was used at the last step to stain the nuclear and mounted with Fluoromount-G (SouthernBiotech, Cat NO 0100-01).
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bioRxiv - Physiology 2020Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech).
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bioRxiv - Microbiology 2020Quote: ... Guts were washed 5 times in PBS-T and then mounted on slides in Flouromount plus 4’,6-diamidino-2-phenylindole nuclear stain (DAPI, Southern Biotech) and sealed with a cover slip ...
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bioRxiv - Immunology 2021Quote: ... DAPI was used as a nuclear counterstain and slides were mounted with Fluoromount-G (0100-01; Southern Biotech, Birmingham, AL, USA). All incubations steps were performed at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech). CD68 staining was performed according to the Cell Signaling protocol for antibody #97778 ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Immunology 2022Quote: ... and biotin-labeled goat anti-mouse IgA (Cat# 1040-08) were obtained from Southern Biotech (Birmingham, AL). HRP-conjugated anti-mouse IgG Fab (Cat# A9917 ...
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bioRxiv - Molecular Biology 2020Quote: ... and then 4 × 1 hour prior to counterstaining with the nuclear marker (DAPI) (1 ng/ml) and mounting in Fluoromount G (Southern Biotech, Birmingham, AL). The primary antibody used was anti-Schistosoma japonicum Ago2 (clone 650-1-1-KM864-3-11E8 ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).