-
No products found
because this supplier's products are not listed.
Issei S. Shimada, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... and 0.4% Triton X-100 (Sigma-Aldrich, 30-5140-5) in PBS ...
-
No products found
because this supplier's products are not listed.
Marianne C. Kramer, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 100 units SuperScript II and 30 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 2 minutes at 25°C ...
-
No products found
because this supplier's products are not listed.
Laura E. Sanman, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 100 U/mL penicillin and 100 μg/mL streptomycin (Corning #30-002).
-
No products found
because this supplier's products are not listed.
Abdur Rahim, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with Luna 5 μm C18 (2) 100 Å media (Phenomenex, Torrance, CA). The samples were eluted at a flow rate of 0.25 uL/min with a gradient of 0.1% FA in water (Solvent A ...
-
No products found
because this supplier's products are not listed.
Aida Alonso-del Valle, et al.,
bioRxiv - Microbiology 2020
Quote:
... chloramphenicol (30 μg) and amikacin (30 μg) (Bio-Rad, CA, USA). pOXA-48-carrying and pOXA-48-free strains were pre-cultured in Müller-Hinton (MH ...
-
No products found
because this supplier's products are not listed.
Wiebke Nahrendorf, et al.,
bioRxiv - Immunology 2020
Quote:
... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
-
No products found
because this supplier's products are not listed.
Jack Leslie, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... CD45 (Biolegend, 5 µg, 30-F11), CD101 (eBioscience ...
-
No products found
because this supplier's products are not listed.
Gianna Triller, et al.,
bioRxiv - Immunology 2022
Quote:
... 30 mg (Agilent, Santa Clara, CA). Cartridges were first washed with 500 µL methanol followed by 500 µl water and then samples were loaded onto the plate ...
-
No products found
because this supplier's products are not listed.
Shaline V. Fazal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... b-FGF (30 ng/ml, Peprotech, #100-18B) and PDGF (30ng/ml ...
-
No products found
because this supplier's products are not listed.
Manan Krishnamurthy, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... TTF-1 (abcam #ab72876, 1:100 30’), CD56 (Cell Signaling Technology #99746 ...
-
No products found
because this supplier's products are not listed.
Stefan A. Fattinger, et al.,
bioRxiv - Immunology 2022
Quote:
... CD45-BUV563 (BD Biosciences; 30-F11; 1:100), MHCII-BV421 (Biolegend ...
-
No products found
because this supplier's products are not listed.
Pascal Kröger, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Staining was blocked for 30 min with CT buffer (0.5% Triton X-100, 5% ChemiBLOCKER in 0.1M NaP pH 7, Sigma Aldrich & Merck) at RT ...
-
No products found
because this supplier's products are not listed.
Dominik Groos, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 4-AP (100 µM, Tocris, 0940, CAS: 504-24-5), CNQX (20 µM ...
-
No products found
because this supplier's products are not listed.
Junshi Wang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... tissue was incubated for 30 min in 1:100 avidin-biotin peroxidase complex (Vectastain Elite kit; Vector Laboratories, Burlingame, CA) and rinsed again ...
-
No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 30 μg/mL blasticidin and 100 μg/mL zeocin (InvivoGen). THP1 ...
-
No products found
because this supplier's products are not listed.
Martin Sperfeld, et al.,
bioRxiv - Microbiology 2024
Quote:
... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The lysates were subsequently immunoprecipitated using anti-PGC-1α antibody (D-5) (sc-518025, 1:30, Santa Cruz Biotechnology, CA, USA), anti-ANXA1-antibody (ab214486 ...
-
No products found
because this supplier's products are not listed.
Scott B. Thompson, et al.,
bioRxiv - Immunology 2024
Quote:
... and 30 µl of 5 mg/ml Collagenase D (Roche) and 100 µl of 2 mg/ml DNase I (Worthington Biochemical ...
-
No products found
because this supplier's products are not listed.
Raquel R. Martins, et al.,
bioRxiv - Genomics 2022
Quote:
... 30 μl of chloroform (1:5, VWR International) was added and the samples were incubated for further 3 min at RT before centrifuged at 13,000g ...
-
No products found
because this supplier's products are not listed.
Manan Krishnamurthy, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... CD56 (Cell Signaling Technology #99746, 1:100 30’), INSM1 (Santa Cruz #sc-271408 ...
-
No products found
because this supplier's products are not listed.
Shuaijie Dou, et al.,
bioRxiv - Immunology 2021
Quote:
... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
-
No products found
because this supplier's products are not listed.
Emilia Neuwirt, et al.,
bioRxiv - Immunology 2022
Quote:
... 20 - 30 µM CA-074Me (Calbiochem), 0,4 µM ferrostatin-1 (Biomol) ...
-
No products found
because this supplier's products are not listed.
Brianna L. Petrone, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and spiked with 30% PhiX (Illumina, San Diego, CA) to mitigate low nucleotide diversity ...
-
No products found
because this supplier's products are not listed.
Jessica Ciesla, Isreal Moreno Jr., Joshua Munger,
bioRxiv - Microbiology 2022
Quote:
... Vidofludimus (Cayman Chemicals, Item#18377 CAS# 717824-30-1) suspended in DMSO to 10 mM and prepared to the indicated concentrations in culture medium ...
-
No products found
because this supplier's products are not listed.
Elizabeth E. Ha, et al.,
bioRxiv - Physiology 2021
Quote:
... and 5 μg lentiviral construct with 30 μL Fugene 6 (Promega) the following day ...
-
No products found
because this supplier's products are not listed.
Yi Zhang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The supernatant was then centrifugated at 10,000×g for 30 min followed by ultracentrifugation at 100,000×g (Optima XE-100 Ultracentrifuge, Beckman Coulter, Brea, CA) for 2 h ...
-
No products found
because this supplier's products are not listed.
K. Metz, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and sampled at 30 kHz (Axoscope 8; Axon Instruments /Molecular Devices, Burlingame, CA). These grease gap recordings of PAD on sensory afferents reflect only the response of the largest diameter axons in the dorsal root ...
-
No products found
because this supplier's products are not listed.
Jonathan T. Lloyd, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
-
No products found
because this supplier's products are not listed.
Laurie Lannes, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 100 µL of sample were injected on a Superose 6 30/100 (GE Healthcare) analytical SEC column preequilibrated with the last dialysis buffer ...
-
No products found
because this supplier's products are not listed.
Neesha Kara, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plug was equilibrated 30 min in 100 μl CutSmart buffer (NEB), digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S ...
-
No products found
because this supplier's products are not listed.
Xudong Dong, Ailing Ding, Jiangwei Lin,
bioRxiv - Developmental Biology 2022
Quote:
... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
-
No products found
because this supplier's products are not listed.
Bolin An, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... OD600 readings were recorded every 5 minutes for 30 hours at 30 °C using a microplate reader (Biotek, SYNERGY H1), which included a 10-second shake before each measurement ...
-
No products found
because this supplier's products are not listed.
Emi Nakahara, et al.,
bioRxiv - Biochemistry 2022
Quote:
... roughly 5 million AAV pro293T cells (Takara, San Jose, CA) in 30 mL of media were seeded overnight in a T175 flask (Greiner Bio-One ...
-
No products found
because this supplier's products are not listed.
Tunglin Tsai, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 100 nM dexamethasone (MP Biomedicals, Irvine, CA), 10 mM β-glycerophosphate (Alfa Aesar ...
-
No products found
because this supplier's products are not listed.
Sungjin Oh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The fast-drying silver paint (16040-30, Ted Pella Inc., CA, USA) was injected through the ground via holes at the gold-plated ground pad to electrically short the p-substrate of the CMOS interface IC to the PCB ground ...
-
No products found
because this supplier's products are not listed.
Shannon J. McKie, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 100 μM aminooxy-biotin (Biotium Inc., Hayward, CA) and 10 μM aniline (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Nunya Chotiwan, et al.,
bioRxiv - Microbiology 2022
Quote:
... Glass needles were prepared with a vertical pipette puller (P-30, Sutter Instrument Co., Novato, CA) and mosquitoes were IT injected with 3 µg/µl of dsRNA in an injection volume of 69 nl ...
-
No products found
because this supplier's products are not listed.
Yuanyuan Wang, et al.,
bioRxiv - Neuroscience 2024
Quote:
... + 30 ng/mL IFNγ (R&D systems; 485-MI-100/CF).
-
No products found
because this supplier's products are not listed.
Robert Prior, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 5 µM FSK (STEMCELL Technologies, #100-0249) and 200 ng/ml NRG1 type III ...
-
No products found
because this supplier's products are not listed.
Kim Hoa Ho, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... collected on uncoated nickel grids (100 mesh) and counterstained for 30 seconds with UranyLess EM Stain and 30 seconds with lead citrate (Electron Microscopy Sciences). Sections were observed with a JEM-1400 Flash transmission electron microscope (JEOL ...
-
No products found
because this supplier's products are not listed.
Mary O’Keeffe, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and O4 PE (1:100, Miltenyi Biotec, 30-117-357). ACSA2 APC (+ ...
-
No products found
because this supplier's products are not listed.
Marilia B Malta, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
-
No products found
because this supplier's products are not listed.
Sarah Dagher, Shimpei Ishiyama,
bioRxiv - Neuroscience 2024
Quote:
... two 5 weeks old rats habituated to tickling underwent unilateral injection of 100 nl at 30 nl/min of AAV2/5.hsyn.eGFP (Addgene, 50465-AAV5) in the insular cortex at the following coordinates ...
-
No products found
because this supplier's products are not listed.
Anamika Patel, et al.,
bioRxiv - Immunology 2022
Quote:
... concentrated using a 30 kDa or 100 kDa cut-off concentrator (Vivaspin, Sartorius) and stored at 4°C for further use.
-
No products found
because this supplier's products are not listed.
Jonas Kath, et al.,
bioRxiv - Immunology 2021
Quote:
... or an electroporation cartridge (100 µl = 5×106 cells, Lonza). Cells were carefully transferred onto the electroporation strips using 200 µl tips to avoid trapping air in the solution ...
-
No products found
because this supplier's products are not listed.
Jonathan Tai, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Reactions were carried out at 30 ºC in a 5 mL tube (Eppendorf) with constant shaking (500 rpm) ...
-
No products found
because this supplier's products are not listed.
Samantha L. Sturiale, Nathan W. Bailey,
bioRxiv - Evolutionary Biology 2021
Quote:
... we began recording for 5 minutes at 30 frames/second using a camera (Nikon D3300) mounted ca ...
-
No products found
because this supplier's products are not listed.
Yan Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Protein solutions of 1-5 μM concentration in +Ca or -Ca buffer were prepared and measured using an inverted microscope (IX81, Olympus) equipped with a 60X ...
-
No products found
because this supplier's products are not listed.
Pablo Blanco-Carlon, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 min following incubation with 1:100 dilution of the primary antibody anti- Piezo1 (28511- 1-AP, Proteintech). Samples were scored in five levels of PIEZO1 expression in the tumor (0 to 4) ...
-
No products found
because this supplier's products are not listed.
Agnieszka A. Kendrick, et al.,
bioRxiv - Biophysics 2024
Quote:
... Samples containing ATP were incubated for a total of 0.5 min or 30 min on ice (including blotting time and plunging) and quickly applied to plasma cleaned (Solarus, Gatan) UltrAuFoil Holey Gold R 1.2/1 ...