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Citations for Qiagen :
1 - 50 of 3346 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... using 30 nM Hs_LUZP1 siRNA (target sequence, 5′-CAGCGGGTGCTGAGAATTGAA-3′; QIAGEN) or 30 nM AllStars negative-control siRNA (QIAGEN) ...
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bioRxiv - Physiology 2024Quote: ... was mechanically homogenised in TRI reagent with a 5 mm steel bead at 30 Hz for 2 x 30 s (TissueLyser II, Qiagen) then centrifuged for 10 min at 10,000 g ...
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bioRxiv - Immunology 2023Quote: ... Tissue RNA was isolated from preserved samples using 30 minutes of automated homogenization at 30 Hz with 5 mm ball-bearings in RLT Plus Lysis Buffer (Qiagen TissueLyser II), RNA was then isolated using a RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... Residual contaminant DNA was removed using 30 U RNase-free DNase (Qiagen CA, USA), and elution was performed with 40 μl of RNase-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... or 100 nM FlexiTube siRNA to ARF6 (Qiagen, Valencia, CA) using 9.5 μl of Oligofectamine (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... or 100 nM FlexiTube siRNA to ARF6 (Qiagen, Valencia, CA) using 9.5 μl of Oligofectamine (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... or 100 nM FlexiTube siRNA to ARF6 (Qiagen, Valencia, CA) for 72 hours prior to plating and then seeded at 2×104 cells/well in complete media ...
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bioRxiv - Physiology 2022Quote: ... Samples were homogenized using a TissueLyser II device (Qiagen; 5 min at 30 pulses/second) in 425 μL water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.1 g of tissue per 1 mL of PBS was homogenized with stainless steel beads of 5 mm (25–30 Hz for 5 min) by a Tissuelyser (Qiagen, Venlo, Netherlands). The homogenates were centrifuged at 10,000 x g for 10 min and the supernatant was collected and stored at −80 °C until CD quantification ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μg of the isolated total RNA was treated with 30 U of DNase I (QIAGEN) and purified using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with two 5 mm stainless beads using a TissueLyser (Qiagen, CA, USA) at 50 Hz for 4 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total RNA was extracted from each organ (<30 mg) using RNeasy Mini kits (Qiagen Inc., Valencia, CA). Quantity and purity of total RNA was measured using a Take3 Microvolume Plate and the Take3 app (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and 5 units lysostaphin and mutanolysin for 30 minutes prior to beadbeating in the TissueLyser II (QIAGEN) for 2 x 3 minutes at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... and 5 units lysostaphin and mutanolysin for 30 minutes prior to beadbeating in the TissueLyser II (QIAGEN) for 2 x 3 minutes at 30 Hz ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Neuroscience 2020Quote: ... for 30s at 30 Hz using TissueLyserII (QIAGEN). Lung homogenates were clarified by centrifugation (2,000 × g at 4°C for 10 min) ...
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bioRxiv - Zoology 2021Quote: ... 0.1 μL of 5 U/μL Taq DNA Polymerase (QIAGEN, Valencia, CA, USA), 0.2 μL of U19 fluorescent dye ...
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bioRxiv - Microbiology 2023Quote: ... or a 1.5 ml tube with 5 volumes of RNAlater (Qiagen, Valencia, CA) and preserved at -80 °C for RT-PCR.
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 100 µL DTT (100 mM) at RT for 5 min and recovered by the RNeasy clean up kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Neuroscience 2020Quote: ... 30-40 mg of the sample were incubated in 100 μl of Extraction Buffer EXB Plus (Qiagen, Germany) containing 6% β-mercaptoethanol on ice for 5 min and mixed by vortexing ...
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bioRxiv - Biophysics 2022Quote: ... The suspension was spun down at 16,000 r.c.f at 4 °C for 30 min and the supernatant was applied 5 ml Ni-NTA resin (Qiagen)/ L culture medium ...
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bioRxiv - Microbiology 2023Quote: ... Edmund Bühler) by bead beating twice for 5 min at 30 Hz in a Tissue Lyzer II (QIAGEN). Lysates were then incubated for 30 min at 37 °C with shaking ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina ...
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bioRxiv - Cancer Biology 2022Quote: A 100 µL slurry of 5% Ni-NTA magnetic beads (Qiagen, part# 36113) was placed in 12 1.5 mL Eppendorf tubes and the tubes were placed in a magnetic stand and the supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 g for 10 minutes at 4°C ...
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bioRxiv - Physiology 2020Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were homogenized at 25–30 Hz for 5 min using a tissue homogenizer (QIAGEN TissueLyzer II, Hilden, Germany). Samples were swabbed with sterile dual tip swabs (BD swubes ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The sonicate was centrifuged at 38,000 × g for 30 min and the supernatant was loaded on a Ni-NTA agarose column (2.5 × 5 cm, Qiagen) pre-equilibrated with buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by centrifugation at 20,000 x g for 30 min and loaded on a 5 ml Ni-NTA Superflow column (QIAGEN) followed by extensive washing ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 30,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Microbiology 2020Quote: ... region was amplified using fungal-specific primers (60): ITS1F (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and the HotStarTaq Plus Master Kit (Qiagen, Valencia, CA). Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads (Agencourt Bioscience Corporation ...
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bioRxiv - Physiology 2020Quote: ... RNA was isolated from ~20-30 mg of liver tissue using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA), with cDNA produced using the ImProm-II RT system (Promega ...
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bioRxiv - Plant Biology 2021Quote: Tissues (100 mg) were homogenized into powders using a TissueLyser II (Qiagen, Valencia, CA, USA). DNA was extracted using the DNeasy Plant kit (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... Exosome samples were treated with 1 µL RNAse A/T1 per 100 µL sample for 30 min at 37°C and lysed in 650 µL of QIAzol (Qiagen). To monitor the RNA recovery percentage as a function of the isolation procedure ...
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bioRxiv - Cancer Biology 2022Quote: ... Library plasmid pools were propagated in liquid culture in LB with ampicillin (100 μg/ml) at 30 °C overnight and extracted (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: ... The entire flies were mechanically crushed for 30 s at 25 Hz using a 5-mm stainless steel bead in a TissueLyser (Qiagen). Three hundred μL of ACL solution and 20 μL of 16 g.L-1 proteinase K were then added to the samples ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was collected from multiple stages of zebrafish development (30%-epiboly, 9 somite, Prim-5, High-pec and Larvae) using the miRNeasy kit (Qiagen), according to the manufacturer’s instructions ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Lysates were cleared by centrifugation at 15000 x g for 30 min at 4 °C and incubated in Pierce 5 ml columns with Ni-NTA agarose beads (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated for 30 min at 4 °C with 5 mL of Ni2+-beads (Qiagen Ni-NTA Superflow) equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...