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Citations for GE Life Sciences :
1 - 50 of 2283 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 100 µL of sample were injected on a Superose 6 30/100 (GE Healthcare) analytical SEC column preequilibrated with the last dialysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were concentrated to 100 µL and purified by SEC (Superose 6 30/100, GE Healthcare) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The three samples were analyzed by SEC (Superose 6 30/100, GE Healthcare) in their respective buffer ...
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bioRxiv - Biochemistry 2021Quote: 100 μL protein samples were injected onto an S200 10/30 column (GE Healthcare) equilibrated in a running buffer of 10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were resuspended in 30% Percoll (100% Percoll Plus, 17-5445-02, GE Healthcare) and centrifuged at 300g for 15 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed with a Superdex 200 30/100 GL column (GE Healthcare). 100 μL of 10–100 μM purified proteins were loaded and eluted at a flow rate of 0.5 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... the enzyme was applied to a Superdex 200 30/100 gel filtration column (GE Healthcare) equilibrated in protein storage buffer (20 mM HEPES pH 7.8 ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Immunology 2021Quote: ... debris and aggregates were removed using a 100/60/40/30-percent gradient of Percoll (GE HealthCare) in RPMI 1640 (Lonza) ...
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bioRxiv - Biochemistry 2020Quote: Protein (450 μg/100 μL) was loaded on a Superdex™ 200 HR 10/30 column (GE Healthcare) equilibrated with PBS (140 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of alkylation solution [100 mM iodoacetamide (GE Healthcare, Chicago, IL, USA)] was added and incubated protected from light for 15 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 30 min) and loaded onto a 5 ml HighTrap MBP column (Cytiva) using an Äkta-Pure system (GE Healthcare). The column was washed with 10 column volumes of lysis buffer and protein eluted with MalE-elution buffer (100 mM Tris-HCl pH7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of FN3Con-anti-VEGFR2 was measured using surface plasmon resonance with a 30 µl/min flow rate at 25 °C (BIAcore T-100, GE Healthcare). VEGFR2 domains (SiboBiological ...
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bioRxiv - Plant Biology 2024Quote: ... The enriched nuclei were further purified using three-step Percoll-gradients (100%/60%/30%, 17-0891-01, GE Healthcare, Solingen, Germany) modified after Marienfeld et al ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer salt concentration was diluted to 30 mM NaCl and the solution was flown through a 5 mL HiTrap Q HP (GE Healthcare) and a NiNTA column ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.0 and 5 mM β-mercaptoethanol) containing 100 mM NaCl by using desalting column (GE Healthcare) and further purified by affinity chromatography with HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Genomics 2020Quote: ... The mixture was left to react for 30 minutes protected from ambient light and the crude reaction mixture was passed through a NAP-5 column (GE Healthcare Life Sciences, 17085301) for collection and purification of the fluorophore-conjugated secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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bioRxiv - Bioengineering 2022Quote: ... a 30% isotonic Percoll (GE Healthcare), underlaid with 70% isotonic Percoll and spun at 650 G for 25 minutes with no brake separated red blood cells ...
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bioRxiv - Immunology 2024Quote: ... suspended in 30% Percoll (GE Healthcare) and then spun at 1590g for 30min without brake ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 30% Percoll (GE Healthcare)-HBSS containing 1μM BBG ...
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were incubated at 30°C for 30 min and subsequently spotted onto Hybond-XL membrane (GE Healthcare). Each membrane was immersed in Ultima Gold (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Biochemistry 2021Quote: ... peak fractions were diluted to 100 mM NaCl and loaded onto a 5 ml HiTrap Q XL column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended in 30% Percoll (GE Healthcare, USA), and loaded onto 70% Percoll (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Superdex 200 PC 3.2/30 (GE Healthcare) column was used with flow rate of 0.5 ml/min in 30 mM Tris HCl pH7.5 ...