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Citations for New England Biolabs :
1 - 50 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... plug was equilibrated 30 min in 100 μl CutSmart buffer (NEB), digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S ...
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bioRxiv - Molecular Biology 2020Quote: ... a plug was equilibrated 30 min in 100 μl 1x CutSmart buffer (NEB), digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB), followed by ligation reaction at 16°C for 4.5 h and then at room temperature for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... First the 5’ triphosphate of RNA was converted into a 5’ monophosphate by incubating 100 µg RNA with 100 units of RNA 5’ Pyrophosphohydrolase (NEB, Ipswich MA) at 37°C for 1 hour within a 100 µl reaction volume ...
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bioRxiv - Plant Biology 2024Quote: ... for 30 minutes and the supernatant mixed with 5 mL Chitin Resin (NEB #S6651), previously washed first with 30 mL of ddH2O and then 20 mL of HEGX buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Purified DNA was treated with 100 nM TDP2 in 30 µL of 1X rCutsmart buffer (NEB) and incubated at 37°C for 180 min to completely remove the 5’ phosphotyrosine adducts ...
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bioRxiv - Genomics 2020Quote: ... and SalI (5’-GTCGAC-3’, New England Biolabs Inc., MA, CA#R3138S) restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... NH4HCO3 (100 mM) and 5 units of alkaline phosphatase (CIP) (NEB, #M0525S) were added and the sample incubated for 2 hours (or 20 min ...
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bioRxiv - Biochemistry 2021Quote: 100 μl extension reactions were performed with Klenow Fragment (3’→5’ exo-) (NEB) (Figures 2B ...
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bioRxiv - Cell Biology 2024Quote: ... Whole brains were blocked at RT for 1 h in PBT containing 0.5% BSA and 5% NGS (PBANG) and then incubated in PBANG containing rabbit anti-GFP (1:100; Torry Pines Biolabs, TP401) at 4°C for 2 nights ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated for 30 min at 37°C in 100 μl A-tailing mix (1x NEB buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... plus 100 µg/mL BSA) and A-tailed (30 min 37°C; NEBNext® dA-Tailing Module; NEB, E6053L) with intervening CutsmartTM washes ...
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bioRxiv - Cancer Biology 2022Quote: ... new SNAP-tagged histones were pulse-labeled for 30 min with 5 μM final SNAP-biotin (New England Biolabs) diluted 1:200 in 10% Duolink blocking buffer (Sigma-Aldrich ...
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bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% Igepal CA-630 and 5% glycerol) with 1 U/µL murine RNase inhibitor (NEB), 0.1 µg/µL T7 RNA polymerase (purified in-house ...
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bioRxiv - Microbiology 2024Quote: ... and 1U RNasin) for 2 hours at 30°C and then incubated with 100 μL of Streptavidin Magnetic Beads (NEB) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The hygR insert and the inverse PCR product of the pEX18Ap backbone were mixed at a 5:1 ratio and ligated for 30 minutes at 50°C with New England Biolabs (NEB) Gibson Assembly ® Cloning Kit to create pEX18HygB ...
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bioRxiv - Cell Biology 2020Quote: ... “Classic” RRBS library preparation was performed by digesting 5 ng of genomic DNA with 30 units of MspI enzyme (New England BioLabs), and fragments were ligated to pre-annealed adapters containing 5’-methyl-cyotosine ...
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bioRxiv - Microbiology 2020Quote: ... The efgA coding sequence plus 30 bp at the 5’ end was amplified using primers with 30 nt overlaps to permit Gibson assembly (HiFi DNA Assembly, New England Biolabs) into pAH120 [40] that had been digested with XbaI and NdeI ...
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bioRxiv - Immunology 2020Quote: ... 70°C for 10s and 72°C for 30s) and 1x 72°C for 5 min) using the Q5 polymerase (New England BioLabs) and cloned into pUC57 using the MEGAWHOP35 method ...
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bioRxiv - Molecular Biology 2022Quote: ... on-bead decapping and phosphorylation were performed in a 30 μl reaction with 5 units T4 PNK 3’ phosphatase minus (NEB), 2.25 μg GST-Dcp1-Edc1-Dcp2 ...
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bioRxiv - Developmental Biology 2020Quote: 5-7 μg of HiC library in a total volume of 100 μl (1x NEB buffer 2.1 ...
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bioRxiv - Biochemistry 2024Quote: In-vitro synthesized pri-miRNAs (100 pmol) were 5’-end labelled by T4-PNK (NEB) using [γ-32P]-ATP (Perkin Elmer ...
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bioRxiv - Genomics 2024Quote: ... and 30 U of T4 RNA Ligase 1 (30 U/mL, NEB). The sample was incubated at 37°C for 1 h.
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...
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bioRxiv - Microbiology 2023Quote: ... Initial PCR amplifications were carried out in a 30 μl mixture that included 5 μl of KAPA HiFi Fidelity Buffer (New England Biolabs, UK), 0.3 μM of forward and reverse primers ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of gDNA was directly digested with 5 U of EcoRI-HF (New England Biolabs) in the ddPCR Supermix for Probes (no dUTPs ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of XBA1 (NEB), 1.5□l of 100mM spermidine and the reaction was made up to 150□l with nuclease free water ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of SSP1 (NEB), 30 units of BSRG1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of BSRG1 (NEB), 30 units of ECoR1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of HindIII (NEB), 30 units of XBA1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of ECoR1 (NEB), 30 units of HindIII (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... with Nt.BbvCI (NEB, 30 U) in 20 µL reactions for one hour at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were then incubated at 30°C for 30 minutes with lambda protein phosphatase (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μl 10X ligation buffer, 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB] ...
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bioRxiv - Genomics 2023Quote: ... 2.5 µl of 100 mM dATP solution and 2.5 µl of Klenow Fragment (3′->5′ exo-) (NEB, #M0212L) were added to the mixture ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei, NEB R0710S) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 μL murine RNase inhibitor (NEB), 45 μL SuperScript III reverse transcriptase (200 U/μL ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 units T4 PNK (NEB). RNA immunoprecipitation was performed with Anti-BrU-conjugated agarose beads (Santa Cruz Biotechnologies) ...
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bioRxiv - Biochemistry 2021Quote: ... or 30 units SacI-HF (NEB). Reactions containing RLP include 4 nM RNase HI ...
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bioRxiv - Microbiology 2022Quote: ... and 30 units BsaI-HFv2 (NEB). The reaction was incubated 90 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 30 U Exonuclease V (RecBCD, NEB), and 2 mM ATP ...
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bioRxiv - Plant Biology 2021Quote: ... Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB). DNA was recovered after reverse crosslinking and Proteinase K treatment (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Cells were fixed with 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2mM Vanadylribonucleoside complex (New England Biolabs). Coverslips were preserved in 70% EtOH at -20°C ...
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bioRxiv - Genomics 2022Quote: ... a total of 100 ng of SED vector construct was digested with 5 U of I-SceI meganuclease (NEB) at 37°C for 10 minutes in a 10 μL reaction mixture ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... the beads were resuspended in TEV buffer (final volume 5 mL) with 100 μL TEV protease (New England BioLabs, #P8112S) and incubated at 4°C on a roller overnight ...
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bioRxiv - Microbiology 2020Quote: ... 30 μL of 10x CutSmart buffer (NEB), and 4 μL of EcoRI-HF or BamHI-HF restriction enzyme (20,000 units/mL)] for 30 min at 37°C.