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Citations for Roche :
1 - 50 of 3046 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 30 µl of 5 mg/ml Collagenase D (Roche) and 100 µl of 2 mg/ml DNase I (Worthington Biochemical ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES, pH 7.5, 300 mM NaCl, 5% glycerol, 30 mM imidazole, Complete EDTA-free protease inhibitor [Roche]) and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... 300 mM NaCl, 30 mM imidazole, 1 M urea, 1% v/v Triton X-100, 5 mM beta-mercaptoethanol, with Roche EDTA-free cOmplete protease inhibitor) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt, Roche). After development ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mM KCl, 5 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, and 0.1% Triton X-100 supplemented with Roche Protease Inhibitor cocktail) and then roc ked for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... or for 30 min at 37°C in 100 μg/ml Liberase (Roche, 5401127001), in serum-free DMEM (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% IGEPAL CA-630 supplemented with cOmplete protease inhibitors (Roche), and incubated on ice for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... 5 mM CaCl2 and 5 mM MgCl2 with 100× concentrated EDTA-free protease inhibitor cocktail (Roche, pallet). The suspensions were treated with Dounce and added with 25 mU ml−1 apyrase ...
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bioRxiv - Genomics 2024Quote: ... 5% Triton X-100) supplemented with proteinase inhibitors (Roche Cat #118733580001). Chromatin was sheared to 100-300bp fragments on a Bioruptor pico sonicator (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then treated with DNase-free RNase (Roche; 5 μg.ml-1; 37°C; 30 min) and proteinase K (250 μg.ml-1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 mM mgCl2 x 6 H2O and 5 mg/mL glucose-6-phosphate dehydrogenase (Roche Diagnostics) in 0.1 M potassium phosphate buffer pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were thawed at room temperature for 30 min and blocked with 5% BSA (Roche) + 0.2% Tween 20 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
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bioRxiv - Microbiology 2022Quote: ... The final library pool of 30 samples was quantified with the Kapa Biosystems (Roche Sequencing; Pleasanton, CA) qPCR protocol ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100 and 5% glycerol) supplemented with protease inhibitor (Roche), 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen ...
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bioRxiv - Microbiology 2024Quote: ... 1% Triton X-100 and 5% glycerol) supplemented with protease inhibitor (Roche), 1 μL/mL Ready-LyseTM Lysozyme Solution (Lucigen ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Genetics 2021Quote: ... and the cell pellet resuspended in cold lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 1% Triton X-100, cOmplete protease inhibitor cocktail, Roche) and incubated on ice for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Pathology 2024Quote: ... Slides were incubated for 30 minutes with 1 unit of DNase I (Roche, Cas. No. 9003-98-9) at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... 5% d8-13C depleted glycerol, 1 mM EDTA, 5 ug/mL of aprotinin and leupeptin and 100 µg/mL Roche protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.5, 150 mM NaCl, 5% glycerol, 1% Triton X-100, 1□mM EDTA, 1X Complete Mini protease inhibitor cocktail [Roche]). Tissues were disrupted using a mechanical homogenizer (IKA T10 basic ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 / 30 ng (immunoprecipitation/input) used for library preparation using KAPA LTP library preparation kit (KAPA Biosystems). Library preparation was stopped prior to PCR amplification ...
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bioRxiv - Plant Biology 2021Quote: ... 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... These samples were then mixed with an equal volume of 2-fold lysis buffer (2% TX-100, 20 mM MES, 30 mM Tris, pH 7.4, 20 mM DTT, 1x protease inhibitor cocktail [PIs, Roche cOmplete] with 0.2 M ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Biochemistry 2023Quote: ... Pelleted cells were resuspended in 20-30 mL Wash Buffer (150 mM NaCl, 100 mM Tris, pH 8.0) and a cOmplete protease inhibitor tablet (Roche) was added ...
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bioRxiv - Microbiology 2020Quote: ... 300 mM NaCl, 30 mM imidazole, 1 μM DNases, 0.75% CHAPS, 5[mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]), and lysed with high pressure cell homogenizer (Union-Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... Lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, one tablet of Roche complete protease inhibitor). We used salt-free (NaCl-free ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris pH 7.5, 50 mM potassium acetate, 5 % v/v glycerol, 0.3 % v/v Triton X-100, one tablet of Roche complete protease inhibitor) 25,27,35 ...
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bioRxiv - Immunology 2022Quote: ... Excised tumors were cut into small pieces and digested at 37°C for 30 min with 100 μg/ml DNase I (Roche) and 200 U/ml Collagenase (Worthington) ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were lysed on ice for 30 min in 100 – 200 µl RIPA buffer supplemented with 1X cOmplete protease inhibitor cocktail (Roche). EVs were directly lysed in PBS/0.1% Triton-×100 ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001; Roche). Then DNAse I was inactivated by addition of 1 μl of 25 mM EDTA and incubation at 70°C for 10 min.
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s at 72°C) and final extension 5 min at 72°C using KAPA Taq DNA polymerase (KAPA BIOSYSTEMS). PCR products were visualized on 2% agarose gels containing SYBR Safe DNA gel stain (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... 5% glycerol) containing 100 mM Phenylmethylsulphonylfluoride (PMSF) and EDTA-free Protease inhibitor cocktail tablets (Roche). Resuspended cells were first treated with 1 mg/ml Lysozyme to digest the cell wall and subsequently frozen at −80°C overnight ...
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bioRxiv - Physiology 2023Quote: ... Worms were homogenized in PBS containing 5% TritonX-100 and a protease inhibitor cocktail (Roche), and lipid was extracted using the TissueLyser II (QIAGEN ...
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bioRxiv - Biochemistry 2022Quote: ... 15 µg total protein per sample was reduced (10 mM DTT, 30 min, 30 °C), carbamidomethylated (55 mM CAA, 30 min, room temperature) and digestion with trypsin (proteomics grade, Roche) overnight at 37°C at a 1:50 enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
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bioRxiv - Plant Biology 2020Quote: ... Bacteria were pelleted and resuspended in lysis buffer (30 mM Tris pH 7.5, 450 mM NaCl, 5 mM β-mercaptoethanol, complete™ EDTA-free Protease Inhibitor Cocktail (Roche) and lysed by sonication ...
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bioRxiv - Bioengineering 2020Quote: ... 30 mM phosphoenolpyruvate (Roche), 130 mM potassium glutamate ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µl of purified DNA is processed using the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... Target cells with PBMCs in complete medium or supplemented with 5% Triton X-100 (Roche Diagnostics) were used to determine minimum and maximum release ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Plant Biology 2023Quote: ... 80 mM KCl, 0.2 mM spermine, 5 mM 2-ME, 0.5 mM spermidine, 0.2% IGEPAL CA-630, Roche mini EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, pH 7.5; 5 mM EDTA; 0.5% Igepal-CA630; 1.0% Triton X-100; protease inhibitors [Roche]) and debris was removed by centrifugation ...