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No products found
because this supplier's products are not listed.
Blessy Paul, et al.,
bioRxiv - Biochemistry 2022
Quote:
The human SNX25 open reading frame (ORF) cloned into the pcDNA3.1+-C DYK was obtained from Genscript (NM_031953). This expresses a C-terminally tagged SNX25 (SNX25-FLAG ...
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No products found
because this supplier's products are not listed.
Ryan S. Lach, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human APC open reading frame purchased from Addgene (#16507), tdmirfp670nano from Max Wilson ...
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No products found
because this supplier's products are not listed.
David Paz, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Missense mutation was introduced into the open reading frame using the QuikChange Site Directed mutagenesis kit (c.440G>A) (Fisher Scientific) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Kathryn M. Spitler, et al.,
bioRxiv - Physiology 2020
Quote:
... A V5 tag was appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs) to generate a V5 tagged version of mouse ANGPTL4 (pHS5) ...
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No products found
because this supplier's products are not listed.
Auke B.C. Otten, et al.,
bioRxiv - Cell Biology 2021
Quote:
... open reading frames were PCR-amplified using CloneAmp HiFi PCR Premix (Clontech), gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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No products found
because this supplier's products are not listed.
Yosuke Fukutani, et al.,
bioRxiv - Neuroscience 2022
Quote:
Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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No products found
because this supplier's products are not listed.
Marija Kuna, et al.,
bioRxiv - Developmental Biology 2022
Quote:
Lenti-X 293T cells were transiently transfected with a CITED2 (NM_001168388) human c-Myc and DYKDDDDK (DDK) tagged open reading frame clone (RC229801, Origene) using Attractene in DMEM medium supplemented with 100 U/ml penicillin ...
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No products found
because this supplier's products are not listed.
Raphaël Pantier, et al.,
bioRxiv - Biochemistry 2020
Quote:
... TET1 open reading frame was subcloned into pRSFDuet plasmids (Novagen) for exogenous expression of MBP-tagged proteins in E ...
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No products found
because this supplier's products are not listed.
Bas de Wolf, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Human Ribo-Zero rRNA depletion kit (Illumina). Paired-end 150+150 bp sequencing was done with Illumina NextSeq 500 using NextSeq 500/550 High Output Kit v2.5 for HeLa samples and with Illumina NovaSeq 6000 using partial S4 flow cell lane for patient samples.
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No products found
because this supplier's products are not listed.
Marshall Lukacs, et al.,
bioRxiv - Genetics 2019
Quote:
... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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No products found
because this supplier's products are not listed.
Yuehui Zhang, et al.,
bioRxiv - Physiology 2020
Quote:
... and MOTS-c levels were assessed using a GSH/GSH+glutathione disulfide (GSSG) assay kit (ab239709, Abcam, Cambridge, UK), MDA assay kit (ab118970 ...
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No products found
because this supplier's products are not listed.
Chrysa Koukorava, et al.,
bioRxiv - Cell Biology 2023
Quote:
... human cardiac microvascular endothelial cells (HCMECs, C-12285), human cardiac fibroblasts (HCFs, C-12375) and human pericytes (PCs, C-12980) were purchased from PromoCell (Heidelberg, Germany), human keratinocyte cells (HaCaTs ...
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No products found
because this supplier's products are not listed.
Srijan Jhingan, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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No products found
because this supplier's products are not listed.
Damelys Calderon, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...
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No products found
because this supplier's products are not listed.
Houqing Yu, Roarke A. Kamber, Vladimir Denic,
bioRxiv - Molecular Biology 2021
Quote:
... Full-length open reading frames or truncated forms of the target proteins were inserted into pGADT7 (AD) or pGBKT7 (BD) vectors (Clontech Laboratories) ...
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No products found
because this supplier's products are not listed.
Lindsey A. Ebke, et al.,
bioRxiv - Cell Biology 2021
Quote:
... ubiquitous mitochondrial creatine kinase (uMtCK C-18, Santa Cruz, 1:500), synaptophysin (SVP38 ...
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No products found
because this supplier's products are not listed.
Yuki Honda Keith, et al.,
bioRxiv - Immunology 2022
Quote:
... anti-human/mouse CD117 (c-kit) antibody (R&D Systems Minneapolis, MN) and anti-CD31 antibody (MEC13.3 ...
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No products found
because this supplier's products are not listed.
Maïté Courel, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... rRNA was depleted using the Ribo-Zero kit Human/Mouse/Rat (Epicentre), and libraries were prepared using random priming ...
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No products found
because this supplier's products are not listed.
Leah S. VandenBosch, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Mice having EGFP knocked-into the Sox2 open reading frame were obtained from Jackson Laboratories (Stock: 017592) and bred to generate P2 litters(Arnold et al ...
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No products found
because this supplier's products are not listed.
Marta Fontcuberta-PiSunyer, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and C-peptide using a human C-peptide ELISA kit (Mercodia, Uppsala, Sweden).
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No products found
because this supplier's products are not listed.
Louis-Félix Nothias, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 12°C in a ThermoMixer (Eppendorf), and centrifuging for 5 minutes at 12000 rpm ...
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No products found
because this supplier's products are not listed.
Joseph C. Reynolds, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and incubated in primary antibodies against MOTS-c (rabbit polyclonal; YenZym, USA) and GAPDH (cat# 5174; Cell Signaling, USA) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Hoai Thi Thu Tran, et al.,
bioRxiv - Microbiology 2021
Quote:
... supernatants were collected and stored at −80 °C until analysis for cytokine secretion using the human MACSplex cytokine 12-kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Petr Šulc, et al.,
bioRxiv - Systems Biology 2023
Quote:
... with AnyDeplete Probe Mix-Human rRNA (Tecan, #S02305). DNase treatment preceded cDNA synthesis ...
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No products found
because this supplier's products are not listed.
Daniel W. Sirkis, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and samples were held at 12°C in the C1000 thermocycler (Bio-Rad) prior to analysis on the droplet reader ...
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No products found
because this supplier's products are not listed.
G Vilema-Enríquez, et al.,
bioRxiv - Neuroscience 2020
Quote:
The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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No products found
because this supplier's products are not listed.
Lukas Weiß, et al.,
bioRxiv - Plant Biology 2021
Quote:
... but in frame with a C-terminal 6xHIS (Novagen, Merck KGaA, Darmstadt, Germany). This way ...
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No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
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No products found
because this supplier's products are not listed.
Fanny Gonzales, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... (kit C, W001; Lonza) and CD81 positive cells sorted (ARIA III BD ...
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No products found
because this supplier's products are not listed.
M.E. McGrath, et al.,
bioRxiv - Microbiology 2022
Quote:
... 12-week old BALB/c (Charles River Laboratories, Wilmington, MA) and 12-week old hACE2 transgenic K18 mice (K18-hACE2 ...
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No products found
because this supplier's products are not listed.
Carmen Camara, et al.,
bioRxiv - Immunology 2021
Quote:
The ACCESS SARS-CoV-2 CLIA (Beckman Coulter Inc., California, USA) was used for semiquantitative detection of IgG directed against S protein RBD using serum obtained from venipuncture blood ...
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No products found
because this supplier's products are not listed.
Wan-ting He, et al.,
bioRxiv - Immunology 2022
Quote:
... 12 month old female Balb/c mice (strain 047) were purchased from Envigo. Mice were housed in individually ventilated Seal-Safe cages ...
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No products found
because this supplier's products are not listed.
Nieves Fernández-Gallego, et al.,
bioRxiv - Immunology 2023
Quote:
Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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No products found
because this supplier's products are not listed.
Darko Bosnakovski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... A codon optimized version of the ENSOANG00000047618 open reading frame was synthesized by Biomatik. cDNAs for DUXA and DUXB were synthesized by Integrated DNA Technologies ...
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No products found
because this supplier's products are not listed.
Xiaoxiao Zhang, et al.,
bioRxiv - Biochemistry 2024
Quote:
Complete open reading frame of murine pro-CtsK (GE Dharmacon) were cloned into pUNO1 (Invivogen) expression vector using AgeI and NheI restriction sites ...
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No products found
because this supplier's products are not listed.
Julia L. de Amorim, et al.,
bioRxiv - Biochemistry 2023
Quote:
The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
-
No products found
because this supplier's products are not listed.
Ishan Goswami, et al.,
bioRxiv - Bioengineering 2022
Quote:
... C-peptide was measured via STELLUX Chemi Human C-peptide ELISA kit (Alpco). Stimulation index (SI ...
-
No products found
because this supplier's products are not listed.
Kristin M. Jacob, Gemma Reguera,
bioRxiv - Microbiology 2021
Quote:
... Growth was initiated with the addition of 18 µl of the inoculum to 162 µl of TSB per well and monitored spectrophotometrically every 30 min (OD630 readings after 0.1 sec of gentle agitation) while incubating the plates at 37°C inside a PowerWave HT (BioTek) plate reader ...
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No products found
because this supplier's products are not listed.
Ik-Jung Kim, et al.,
bioRxiv - Microbiology 2022
Quote:
... The GFP-Flag was constructed by replacing the ORF8-Strep of ORF8-Strep with the GFP-Flag open reading frame sequence (Sino Biological). ORF8-Flag I9P was constructed by replacing ATT with CCT at I9 of ORF8-Flag ...
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No products found
because this supplier's products are not listed.
David Böhringer, et al.,
bioRxiv - Biophysics 2023
Quote:
... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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No products found
because this supplier's products are not listed.
Michela Winters, et al.,
bioRxiv - Microbiology 2021
Quote:
... Wells of the 12-well microtiter plate (VWR 734-2324 T C-treated 12-well plates) were prepared just before inoculation and imaging by coating the bottom of each well with 15 µl of Con A and allowing the wells to dry ...
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No products found
because this supplier's products are not listed.
Mona Bensalah, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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No products found
because this supplier's products are not listed.
Anne R. Shim, et al.,
bioRxiv - Biophysics 2024
Quote:
... Fixed cells were imaged at room temperature (22°C) in trace CO2 (open air) conditions using type N immersion oil (Nikon, #MXA22203).
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No products found
because this supplier's products are not listed.
Elias R. Gerrick, et al.,
bioRxiv - Microbiology 2023
Quote:
RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
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No products found
because this supplier's products are not listed.
Ravi D. Nath, et al.,
bioRxiv - Genetics 2022
Quote:
... at 4°C and then washed for 12 hrs in 1xPBS (Corning, 21-040-CV) at 4°C with three washes ...
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No products found
because this supplier's products are not listed.
Marie-France Dorion, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and C-X3-C motif chemokine ligand 1 (Peprotech) were also added to the culture ...
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No products found
because this supplier's products are not listed.
Carolyn G. Sweeney, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the mRit2 coding region was PCR-amplified and subcloned in-frame into the pTagRFP-C vector (Evrogen) at HindIII/XbaI sites ...
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No products found
because this supplier's products are not listed.
Wesley R. Evans, et al.,
bioRxiv - Neuroscience 2024
Quote:
... mice were deeply anesthetized under 4% isoflurane and then mounted on a heated stereotaxic frame (∼37°C, Stoelting). Anesthesia was maintained with 1–2% isoflurane and adjusted as needed ...
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No products found
because this supplier's products are not listed.
Eva K. Oernbo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and placed in a POCmini2 open perfusion chamber (PeCon) mounted in a 37 °C heating chamber of an inverted confocal microscope (Carl Zeiss, Cell-Observer with a Yokogawa X1 spinning disk ...
-
No products found
because this supplier's products are not listed.
Zijian Fang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Cells were then incubated for 24 hours at 4°C before reading by time-resolved Förster resonance energy transfer (TR-FRET) in a Flexstation 3 plate reader (Molecular Devices) using the following settings ...