Labshake search
Citations for Qiagen :
1 - 50 of 10000+ citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
-
bioRxiv - Genomics 2022Quote: ... the input total RNA was first processed with the QIAseq FastSelect rRNA Yeast kit to remove cytoplasmic and mitochondrial rRNA (Qiagen, CAT. 334215). Library quality was assessed using the ZAG 110 dsDNA kit on (Agilent ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from either 2D or matrigel-on-top (MoT) 3D (protocol for MoT 3D culture is detailed in (22)) cultures using RNeasy mini kit (Qiagen, catalog #74104) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... the effect of human rRNA removal was also assessed using a QIAseq FastSelect rRNA removal kit (Qiagen). The rRNA removal reaction was performed after RNA thermal denaturation at 95℃ 5 min according to the instructions.
-
bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
-
bioRxiv - Developmental Biology 2024Quote: ... using a touchdown PCR for the first 10 cycles from 72 to 60 followed by 35-40 cycles at the proper annealing temperature (Tm −2°C) and extension 68°C 30sec/Kb or 72°C 15sec/Kb and purified using a PCR purification KIT (Qiagen). Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio ...
-
bioRxiv - Genomics 2019Quote: ... with the addition of 20 μl of proteinase K (20 mg/ml) followed by incubation at 56°C for 1-2 hr and the mitochondrial DNA was extracted by Qiagen DNeasy Blood & Tissue Kit (QIAGEN Inc.) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The rRNA depletion strategy using QIAseq FastSelect –rRNA Yeast Kit (Qiagen, #334215) was integrated into the RNA fragmentation step of the TruSeq Stranded mRNA Library Prep kit (Illumina ...
-
bioRxiv - Genomics 2023Quote: ... rRNA depletion was performed using QIAseq FastSelect -rRNA yeast kit (Qiagen #334215) according to the manufacturer instructions ...
-
bioRxiv - Genomics 2022Quote: Mitochondrial DNA was amplified using the REPLI-g Mitochondrial DNA kit (Qiagen, cat no 151023) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: Mitochondrial fractions were purified from tissue and cells using the Qproteome Mitochondrial Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... QIAseq FastSelect rRNA HMR kit (Qiagen) was utilized to minimize rRNA contribution to the final library ...
-
bioRxiv - Immunology 2021Quote: ... was used for rRNA removal using the QIAseq FastSelect –rRNA HMR Kit (Qiagen) in combination with reverse transcription as follows ...
-
bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from ~1.5 x 107 mitochondrial donor cells per mitochondrial transfer using the Qproteome Mitochondrial Isolation Kit (Qiagen, Hilden, Germany, Cat. #37612) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... The rRNA was depleted using the Qiagen QIAseq Fastselect-rRNA/Globin Kit (Qiagen #335377) followed by illumina TruSeq® Stranded Total RNA Library Prep Gold (illumina #20020599) ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: Mitochondrial RNA was isolated from isolated mitochondrial pellet following the manufacturer protocol for the RNeasy Kit (QIAGEN). Isolated mitochondrial RNA was subjected to quality control on Agilent Bioanalyzer and RNA library preparation following Illumina’s RNA-seq Sample Preparation Protocol ...
-
bioRxiv - Genomics 2023Quote: The QIAseq FastSelect rRNA HMR kits (Qiagen) were used to remove rRNA from total RNA and libraries prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
-
bioRxiv - Genetics 2022Quote: ... Ribosomal rRNA was depleted using the QI-Aseq FastSelect –rRNA Yeast Kit (Qiagen cat. 334217). Libraries were sequenced as paired-end 75 base pair reads on a Illumina NextSeq 500 ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2019Quote: We used the REPLI-g mitochondrial DNA kit (Qiagen) to perform WMGA according to the manufacturer’s instruction with the following modifications ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (E6310) or the QIAseq FastSelect -rRNA HMR Kit (Qiagen). RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760 ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by rRNA depletion using QIAseq® FastSelect™−rRNA HMR kit (Qiagen, Germantown, MD, USA); conducted following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... rRNA depletion sequencing library was prepared by using QIAGEN FastSelect rRNA HMR Kit (Qiagen, Hilden, Germany). RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB ...
-
bioRxiv - Immunology 2023Quote: ... rRNA depletion sequencing library was prepared by using QIAGEN FastSelect rRNA HMR Kit (Qiagen, Hilden, Germany). RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... pH 5.8) under long day condtions (16h light) at 24 °C (day) 22 °C (night) using the DNeasy Plant Kit (Qiagen). ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
-
bioRxiv - Microbiology 2023Quote: ... Eluates were then treated with Proteinase K (45°C, 1400rpm, 4h) and RNaseA (37°C, 1400rpm, 30min) before cleanup using MiniElute PCR Purification kit (Qiagen). Purified DNA together with input samples before immunoprecipitation were amplified using qPCR SyBr green mix (PCR Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... 4A-C and 5A-C were lysed in buffer RLT (Qiagen). The cell solution/suspension? was homogenised using QIAshredder columns (Qiagen ...
-
bioRxiv - Genomics 2022Quote: ... 4°C) and stored at −80°C before metagenomic extraction using the DNeasy PowerSoil kit following manufacturer specifications (QIAGEN, Hilden, Germany). All sampling and sample processing was conducted following cold chain principles ...
-
bioRxiv - Molecular Biology 2023Quote: ... and rRNA depleted sequencing library was then prepared using QIAGEN FastSelect rRNA HMR Kit (Qiagen, cat#334378) and NEB Directional Ultra II RNA library prep kit (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... rRNA was depleted using the QIAseq FastSelect rRNA HMR and 5S/16S/23S kits (QIAGEN, Hilden, Germany), and libraries were paired-end sequenced (150 bp ...
-
bioRxiv - Immunology 2023Quote: ... All of the samples were subjected to rRNA removal using the QIAseq FastSelect -rRNA HMR Kit (Qiagen) and cDNA synthesis using Superscript IV (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
-
bioRxiv - Microbiology 2022Quote: ... at −80 °C using the RNeasy plus Mini Kit (Qiagen, 74134) according to the manufacturers protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
-
bioRxiv - Genetics 2019Quote: ... 59°C for 180 s and 72°C for 30 s (Qiagen Multiplex kit handbook ...
-
bioRxiv - Microbiology 2022Quote: ... depletion was performed during library preparation using three probes from QIAGEN FastSelect rRNA 5S/16S/23S Kit (Qiagen, Hilden, Germany), respectively ...
-
bioRxiv - Molecular Biology 2022Quote: ... QIAseq FastSelect -rRNA HMR kit reagent (Qiagen cat# 334386) was added to the first strand cDNA synthesis reaction according to manufacturer’s instructions with the exception that only one tenth the amount was used for the majority of libraries ...
-
bioRxiv - Genomics 2022Quote: ... Depletion of rRNA was performed using FastSelect kits (Qiagen) with probes from both the -rRNA fly and 5S/16S/23S rRNA kits ...
-
bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
-
bioRxiv - Plant Biology 2022Quote: Total root RNA was extracted from plantlets grown in vitro at 22 °C and 10 °C using the RNeasy®Plant Mini Kit (QIAGEN, Germany). One microgram of total RNA was reverse transcribed using an oligo(dT)20 primer and the Super Script™ IV RT (lnvitrogen,USA ...
-
bioRxiv - Microbiology 2023Quote: ... rRNA depletion sequencing library was prepared by using QIAGEN Fastselect rRNA 5S/16S/23S Kit (Qiagen, Hilden, Germany). Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
-
bioRxiv - Neuroscience 2023Quote: ... The next steps included performing rRNA depletion using QIAseq® FastSelectTM−rRNA HMR kit (Qiagen, Germantown, MD, USA), which was conducted following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
-
bioRxiv - Biophysics 2019Quote: ... overnight at 37 °C and purified by a PCR purification kit (Qiagen). Subsequently ...
-
bioRxiv - Neuroscience 2020Quote: ... at −80°C until RNA extraction using the RNeasy Mini Kit (Qiagen). RNA quality was determined using the RNA nano assay on a 2100 Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... pH 7.5 at 4°C) and purified using the RNAeasy kit (Qiagen).
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the QIAseq FastSelect-rRNA Yeast Kit (Qiagen Cat. 334217). Lastly ...