Labshake search
Citations for Bio-Rad :
1 - 50 of 5856 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... and samples were held at 12°C in the C1000 thermocycler (Bio-Rad) prior to analysis on the droplet reader ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
-
bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
-
bioRxiv - Developmental Biology 2020Quote: ... Immuno-Star Western C Kit (BioRad catalog 170-5070) was used ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were heated at 95°C for 5 minutes and loaded on a 12% gel (BioRad). Gels were stained with Coomassie Blue ...
-
bioRxiv - Cell Biology 2023Quote: ... heated for 10 minutes at 95°C and loaded onto 12% SDS polyacrylamide gels (Bio-Rad).
-
bioRxiv - Microbiology 2022Quote: ... 59.2°C and 60°C using PCR thermal cycler (Biorad) for 30 min before HA assay.
-
bioRxiv - Microbiology 2022Quote: ... 59.2°C and 60°C using PCR thermal cycler (Biorad) for 30 min or left at 4°C as control before HA assay.
-
bioRxiv - Plant Biology 2021Quote: ... heated to 60°C for 5 min and electrophoresed into a 12% Tris-HCl Criterion gel from BioRad at 50V for ∼20 min or until the dye front moved just below the bottom of the sample wells ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were further denatured at 80 °C for 5 minutes and loaded in 12% acrylamide gels (Bio-Rad). Electrophoresis was carried in Tris-Acetate SDS running buffer (Bio-Rad ...
-
bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C and separated on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins could be detected with an infrared imager (Odyssey ...
-
bioRxiv - Molecular Biology 2023Quote: ... heated at 95°C for 5 min then run on a 4-12% Bis-Tris gel (BioRad 3450125). The input sample is identical to the initial solution containing protein in 60 μL 1x selection buffer prior to capture with 20 passages over the ME200 tip ...
-
bioRxiv - Biochemistry 2021Quote: ... Mixtures were heated to 90°C for 10 min and slowly cooled to 40°C (0.1°C / second) using a Dyad PCR machine (Bio-Rad).
-
bioRxiv - Biochemistry 2022Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR (Bio-Rad). Melting temperatures were calculated with DSFWorld61 using model 1 in the “by sigmoid fitting” option ...
-
bioRxiv - Neuroscience 2024Quote: ... the iScript™C gDNA Clear cDNA Synthesis Kit (BIO-RAD) was employed ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were incubated overnight at 37°C and the optical density (OD) readings at 595nm were recorded using an iMark™ Microplate Absorbance Reader (Bio-Rad laboratories, Hercules, CA, USA). Synergistic killing of planktonic cells is defined as a greater reduction in viability than can be expected when adding the effects of individual treatments 27.
-
bioRxiv - Biochemistry 2022Quote: ... with a three-step protocol (95°C 15 sec, 60°C 30 sec, 68°C 60 sec) and iTaq Universal SYBR Green Supermix (Bio-Rad). Primer sequences are provided in Table 2 ...
-
bioRxiv - Physiology 2020Quote: ... rabbit polyclonal anti-Cytochrome C (Cyt C) (AHP2302, Bio-Rad Laboratories, 1:500), mouse monoclonal anti-MMP2 (sc-13595 ...
-
bioRxiv - Cell Biology 2021Quote: ... heated for 10 min at 95°C and loaded on Mini Protean TGX precast gel 4-12% (Bio-Rad). For the blot ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 95°C for 10 min and separated by electrophoresis in a 4–12% SDS–PAGE gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
-
bioRxiv - Plant Biology 2022Quote: ... Mitochondrial RNA was purified from the mitochondrial pellets using PureZol (Bio-Rad) and for each reaction ...
-
bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
-
bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).
-
bioRxiv - Molecular Biology 2019Quote: ... containing 2μL of 20X reducing buffer at 95°C for 10 min and separated by SDS-PAGE short migration on 12% SDS-PAGE (Criterion XT Precast gels–BioRad). After a short migration ...
-
bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR was achieved in 12 cycles and at optimized melting temperature (tm) of 68.2 °C thorough C1000 touch thermal cycler (Bio-rad). For single stranded PCR product (ssPCRP ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
-
bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
-
bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
-
bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... Mitochondrial protein concentration was determined using the Dc-protein determination kit (BioRad).
-
bioRxiv - Developmental Biology 2021Quote: ... denaturation at 95°C by 0.5°C in a Real-Time PCR apparatus (BioRad, USA). The genes of interest in this study are listed in supplementary Table 3 ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
-
bioRxiv - Developmental Biology 2019Quote: ... A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad). Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat) ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
-
bioRxiv - Cell Biology 2019Quote: ... The reaction was incubated at 37°C for 30 minutes then left to cool for 10 minutes before adding 6 μL of 0.2% 2-mercaptoethanol in SDS-page loading dye.38 Samples were then heated to 94°C for 2 minutes and added to the wells of a pre-cast 12% polyacrylamide gel (Bio-Rad). The gel was run for 45 minutes at 120 V in running buffer (25 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... separated into an 8%-12% SDS-PAGE gels and transferred to a nitrocellulose Hybond-C membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in PBS-0.1% Tween-20 (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
-
bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
-
bioRxiv - Biochemistry 2022Quote: ... from 20°C to 95 °C in C1000 Thermal Cycler CFX96 Real-time System (Bio-Rad). Tm was determined as a local minimum of the first derivative of the raw fluorescence as function of temperature with software Bio-Rad CFX Manager 3.1.