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Citations for Takara Bio :
1 - 50 of 5263 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... open reading frames were PCR-amplified using CloneAmp HiFi PCR Premix (Clontech), gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The TOP1MT open reading frame (ORF) was cloned using InFusion cloning (Takara, Clonetech) into the pRV100G retroviral vector containing a C-terminal T2A mCherry tag ...
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bioRxiv - Cell Biology 2021Quote: ... the full-length Borr open reading frame was amplified from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... and L gene open reading frames (ORF) were amplified with PrimeSTAR® Max DNA Polymerase (TAKARA) and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Biochemistry 2020Quote: ... The DHTKD1 open reading frame (amino acids 25-919) was amplified using PrimeSTAR GXL DNA Polymerase (Takara) and primers forward (5’-GGT TTA GAA TTC ATG CAG ACC GAG CGG GGC GTT TA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... the JAK3 open reading frame was amplified by PCR using the Advantage 2 polymerase mix (Takara Bio) with JAK3-ORF primers (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid pIRESpuro3-EGFP was constructed by excising the EGFP open reading frame from pEGFP-C1 vector (Clontech) with NheI and BamHI digestion and insertion into the same restriction sites of the pIRESpuro3 vector (Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... the open reading frame was amplified from a larval brain cDNA library using CloneAmp HiFi PCR Premix (Takara Bio). For the HA-tagged version ...
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bioRxiv - Cancer Biology 2022Quote: ... The HAND2 mutant (HAND2mut) open reading frame was cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Developmental Biology 2019Quote: ... Full-length β-catenin and a C-terminal deletion of tle3b (NM_131780, complete reading frame after amino acid 210) were cloned in pGAD (Clontech). Combinations of plasmids to test two-hybrid interactions were co-transformed in Y2Gold yeast strain (Suppl ...
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bioRxiv - Developmental Biology 2021Quote: All novel plasmids constructed for this study were based on the pSP Sox1/2/3> plasmid previously described (9) with the open reading frames replaced by PCR amplifications using a proofreading DNA polymerase (Primestar, Takara) and plasmids were assembled from linear PCR products using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Physiology 2019Quote: ... a tetracycline-regulated Kcnn4 expression construct was generated by subcloning PCR-amplified cDNA encoding the open reading frame of murine Kcnn4 (gene ID16534)) into the pTRE-Tight expression vector (Clontech). The construct was verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... the open reading frame of the OBE3 gene was cloned into XmaI/BamHI sites of the pGADT7 plasmid (Clontech, Palo Alto) resulting into pEW11.
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bioRxiv - Plant Biology 2021Quote: ... For expressing the GAL4BD-SMXL5 fusion protein in yeast the open reading frame of the SMXL5 gene was cloned into XmaI/BamHI sites of the pGBKT7 plasmid (Clontech, Palo Alto) resulting in pEW6 ...
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bioRxiv - Developmental Biology 2024Quote: The open reading frame (ORF) of Kaede was removed from pSPCiPC2Kaede by inverse PCR with PrimeStar GxL DNA polymerase (Takara-bio Inc). The ORFs of Gαq ...
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bioRxiv - Cell Biology 2020Quote: ... The four ALX1 exons encoding the open reading frame were amplified using the CloneAmp HiFi PCR premix (Takara Bio Inc., Kusatsu, Shiga, Japan) and exon specific ALX1 oligonucleotides ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frame encoding each protein was amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa Bio, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Microbiology 2023Quote: The open reading frames of 19 LD-associated proteins were amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Systems Biology 2020Quote: Human mitochondrial to nuclear DNA ratio kit (Takara) was used to assess mitochondrial DNA content ...
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bioRxiv - Developmental Biology 2019Quote: ... plasmid after digestion of the inserts by EcoRI and KpnI and cloning into the EcoRI and EcoRV sites in frame with the C-terminal Flag tag in pSNAPf plasmid using In-Fusion Kit (Clontech). The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the N-terminal YFP tag in EGFp-C1 plasmid using In-Fusion Kit (Clontech) ...
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bioRxiv - Cell Biology 2021Quote: ... The Human Mitochondrial DNA Monitoring Primer Set (TaKaRa, Cat # 7246) was used to determine mitochondrial DNA (mtDNA ...
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Full-length WDR5 was cloned in frame as N- or C-terminal NanoLuc-fusion pNLF1 vector using ligation independent in-fusion cloning (Takara Bio) and sequence verified ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA, 7246).
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bioRxiv - Cancer Biology 2019Quote: ... The open reading frame of AIRE was recovered by PCR using this plasmid as a template and PrimeSTAR GXL DNA Polymerase (Takara, Shiga, Japan). The PCR product was digested with EcoR1 and inserted into pcDNA6-3xFlagN (in-house plasmid made using pcDNA6/V5-HisA (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Removal of rRNA as well as mitochondrial RNA was performed using RiboGone™ - Mammalian (Takara Bio USA, Inc., San Jose, CA) from total RNA according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... and human CaMKIIK42RD135N with a C-terminal AviTag were cloned into pEGFP-N1 (Clontech) vector ...
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bioRxiv - Molecular Biology 2023Quote: ... A mitochondrial DNA monitoring primer set kit from Takara Bio [San Jose ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using primers C.9 and C.10 and cloned into pOPINF using the In-Fusion HD EcoDry cloning kit (Takara Bio).
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bioRxiv - Immunology 2023Quote: Non-tissue culture treated 12-well plates were coated overnight at 4°C with 1 ml Retronectin (Takara) at 25 μg/ml in PBS ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs for C-terminal GFP-tagged human RHBDL4 were generated by subcloning into pEGFP-N1 (Clontech). For generating point mutants ...
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bioRxiv - Cell Biology 2021Quote: ... for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio) for the cells following manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: ... rapalog (A/C Heterodimerizer, Takara), dynapyrazole A (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μM A/C heterodimerizer (Takara) or ethanol control ...
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bioRxiv - Neuroscience 2020Quote: ... Rapalog (A/C Heterodimerizer, TaKaRa, #635056) was added manually to a concentration of 100 nM to the cell medium ...
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bioRxiv - Neuroscience 2020Quote: ... rapalog (A/C Heterodimerizer, TaKaRa, #635056) was added at a final concentration of 100 nM.
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bioRxiv - Cancer Biology 2023Quote: ... were fused to GFP-C (Clontech). mCherry LEGO-C2 was provided by Dr ...
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bioRxiv - Cell Biology 2020Quote: ... at the C-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA fragment of human METTL18 with a C-terminal HA sequence was cloned into AgeI and EcoRI sites of the pQCXIP vector (Clontech). To generate hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-002 encodes a C-terminal FLAG-tagged version of human PKR hosted in the retroviral expression vector pLPCX (Clontech) and was generated by cloning a PCR product (PCRP ...
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bioRxiv - Biochemistry 2023Quote: ... FL-human KANK1 (generous donation from the Bershadsky lab) cDNA was tagged in the C-terminal site with pmCherry (Clontech) by restriction digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... 3Flag-BMAL1 and 3HA-CNOT1-C were transfected by the calcium phosphate-based kit (Clontech) in 10cm plates of HEK293T cell cultures ...
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bioRxiv - Cell Biology 2023Quote: ... Rapalog A/C hetero-dimerizer (635057, Takara), Bafilomycin A1 (sc-201550 ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ...
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bioRxiv - Genomics 2019Quote: ... proof-reading PrimeStar HS DNA Polymerase (Takara) was used following the manufacturer’s instructions ...