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Citations for Promega :
1 - 50 of 4463 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Cell Biology 2019Quote: Open reading frames of OR genes were subcloned into pCI (Promega) with a Rho tag at the N terminal ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-PR open reading frame (ORF) clone was purchased from Promega (Promega #FHC24423). pHalo-ER expresses human ERα fused to HaloTag in the C-terminus regulated by a CMVd1 promoter and has been described previously (21 ...
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bioRxiv - Developmental Biology 2020Quote: Whole open reading frame of eco1 and eco2 where cloned into pGEM-T (Promega). DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche ...
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bioRxiv - Microbiology 2022Quote: ... the mKate2 open reading frame was replaced with that of the nanoluciferase gene from pNL1.2[NlucP] (Promega) and the virus was generated as indicated above ...
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bioRxiv - Immunology 2021Quote: ... The RARα open reading frame (NP_000955) was sub- cloned from the original plasmid (pFN21AE1591, Promega KK, Tokyo, Japan) into the pFC14K plasmid (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... the entire open reading frame was inserted into the plasmid backbone from pFN24A HaloTag CMVd3 Flexi Vector (Promega) so that the ORF was under a CMV-d3 minimal expression promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... was previously constructed by inserting a 351 bp-TiLV segment 9 open reading frame (ORF) into pGEM T-easy vector (Promega) as reported earlier (Thawornwattana et al ...
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bioRxiv - Molecular Biology 2021Quote: ... 3’UTR PCR products were directionally cloned downstream of the Renilla luciferase open reading frame (ORF) of the psiCHECK2 vector (Promega) that also contains a constitutively expressed firefly luciferase gene ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Microbiology 2022Quote: ... from Huh7 genomic DNA and inserted the promoter element upstream of the firefly luciferase open reading frame in the pGL2-Basic vector (Promega Corp.). In all cases ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The resulting RT-PCR amplicons of the stomach sample (full open reading frame sequence for GRP or partial sequence for GRPR) were subcloned into the pGEM-T easy vector (Promega, Madison, WI) followed by transfection into Escherichia coli DH5α competent cells (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Germany)-labeled antisense riboprobe was synthesized from the plasmid containing the full or partial length open reading frame of the cDNAs using SP6 or T7 polymerase (Promega, Madison, WI, USA) after restriction enzyme digestion (New England Bio Labs ...
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bioRxiv - Microbiology 2020Quote: ... We replaced the mCherry reading frame with nano-luciferase (nLuc-VLP) (Promega) or codon optimized GFP (GFP-VLP) ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Neuroscience 2022Quote: ... the used PCR primers were flanked with SfiI and NheI sites to allow the insertion in front of hRluc reading frame in psiCheck-2 vector (Promega).
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bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
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bioRxiv - Microbiology 2021Quote: ... Mitochondrial toxicity assay was performed using the “Mitochondrial ToxGloTM Assay” kit according to the manufacturer’s instructions (Promega). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The standard curve was generated by cloning a short 100-bp sequence of mitochondrial 16S rRNA gene into pGEM®-T Easy Vector (Promega). The 16S_rRNA gene sequence was amplified with Taq Polymerase (Supreme NZYtech II master mix ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Pathology 2019Quote: ... Trypsin digestion occurred in 50 mM ammonium bicarbonate buffer at 37°C for 12 hours (Promega, Trypsin Gold ...
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bioRxiv - Immunology 2023Quote: ... ATP was measured with Mitochondrial ToxGlo Assay kit (Promega) and followed the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 enzyme wt:sample wt to the lavage ...
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 weight:weight to the S-Trap column ...
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bioRxiv - Systems Biology 2022Quote: ... Glu-C (Promega) or Lys-C (Wako chemicals ...
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bioRxiv - Pathology 2024Quote: ... Lys-C (Promega) was added in an enzyme:substrate ratio of 1:75 for 4 hours at 25 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then acidified with 12-21% aqueous phosphoric acid prior to digestion with Trypsin/Lys-C (Promega) and then added immediately to an S-Trap column (Protifi ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested for 2 h at 37°C with Lys-C and 16 h at 37°C with Trypsin Gold (both Promega). For TMT 6-plex labeling (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and digested overnight at 37 °C with Trypsin/Lys-C (Promega, V5071) at a ratio of 1/50 ...
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bioRxiv - Biochemistry 2023Quote: ... overnight at 37°C or sequentially with trypsin and Glu-C (Promega) at an enzyme-to-substrate ratio of 1:20 and 1:50 at 37°C and 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... or Lys-C (Promega). Proteins were resuspended in 100 µl of the appropriate digestion buffer (trypsin ...
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bioRxiv - Developmental Biology 2020Quote: ... Endoproteinase Lys-C (Promega), 5 μL at 1 μg/μL ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... rLys-C (Promega, V1671) added at an enzyme to protein ratio of 1 to 100 (w/w) ...
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bioRxiv - Biochemistry 2021Quote: ... or Lys-C (Promega) at 37 °C for 12 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... endoproteinase Lys-C (Promega) and high-sequencing-grade trypsin ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin/Lys-C (Promega) solution was added and incubated at 37 °C for 12 h ...
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bioRxiv - Microbiology 2020Quote: ... and Glu-C (Promega). Reaction mixtures were dried and peptides/glycopeptides were extracted using C18 Zip-tip (MerckMilipore ...
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bioRxiv - Biochemistry 2022Quote: ... or Glu-C (Promega); aliquots of CD16a proteins were digested respectively using chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... or Glu-C (Promega); aliquots of CD16a proteins were digested respectively using chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... and Glu-C (Promega); aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... or Glu-C (Promega). Next day ...
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bioRxiv - Biochemistry 2023Quote: ... trypsin/Lys-C (Promega) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin/Lys-C (Promega) solution was added ...
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bioRxiv - Genetics 2023Quote: ... and used for thermal cycling (35× 30sec at 95°C, 55°C and 72°C) with 1X GoTaq G2 Master Mixes (Promega, cat.no.M7823), 0.2µM CTNS and 0.2µM bactin1 primers (Supplementary Table S2) ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 37°C or sequentially with trypsin and Glu-C (Promega, UK) at an enzyme-to-substrate ratio of 1:20 and 1:50 at 37°C and 25°C ...
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bioRxiv - Cancer Biology 2021Quote: ... then digested overnight at 37 °C with 1 μg Lys-C (Promega, VA1170) and dried in vacuo ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated at 37 °C for 3 h with Trypsin/Lys-C (Promega) at a 25:1 protein:protease ratio (w/w) ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 μg of Lys-C and 1 μg of Glu-C (Promega, U.S.A.) were used for the sample for quantifying S182 phosphorylation.