-
No products found
because this supplier's products are not listed.
Yuhao Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The OD600 was continuously monitored at 30 °C using Citation 5 (Biotek) every 20 min with constant shaking ...
-
No products found
because this supplier's products are not listed.
Kuan-Ting Huang, et al.,
bioRxiv - Biochemistry 2022
Quote:
Chloramphenicol (Goldbio, cat. no. C-105-5)
-
No products found
because this supplier's products are not listed.
Calvin Yeager, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
-
No products found
because this supplier's products are not listed.
Yakun Wu, et al.,
bioRxiv - Immunology 2021
Quote:
... the samples were diluted 10 x with the Immunoassay Buffer C (Cayman chemical, # 401703). The cGAMP amount was determined by ELISA assays according to the manufacture’s protocols (Cayman chemical ...
-
No products found
because this supplier's products are not listed.
Angelique Peret, et al.,
bioRxiv - Neuroscience 2019
Quote:
... We confirmed that expression of the fosGFP transgene correlated with the synthesis of the endogenous c-fos protein using a c-fos specific antibody (Synaptic Systems GmbH ...
-
No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... guinea pig anti-p62/SQSTM1 (C-terminus) (Progen, GP62-C), mouse anti-SQSTM1 (Abnova ...
-
No products found
because this supplier's products are not listed.
Michelle E. Gray, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
-
No products found
because this supplier's products are not listed.
David W. Hammers, et al.,
bioRxiv - Cell Biology 2021
Quote:
... plasmid was generated by adding the C-terminal 20 amino acids of human H-Ras (NCBI Accession No. NP_005334) to a tagRFP-C vector (Evrogen) modified with a S158T point mutation to enhance photostability (Shaner et al. ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
-
No products found
because this supplier's products are not listed.
Wenjie Wang, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The target protein images were obtained with C-DiGit Blot scanner (LI-COR) and analyzed by ImageStudio (LI-COR) ...
-
No products found
because this supplier's products are not listed.
Anny Devoy, et al.,
bioRxiv - Neuroscience 2021
Quote:
... human specific TDP43 (a mouse monoclonal against the C-terminus of TDP-43 that recognises human TDP-43 protein but not mouse TDP-43, Proteintech 60019-2-Ig, 1:1000), pan SOD1 (a custom-made goat polyclonal antibody recognising a C-terminal epitope of SOD1 that is identical between mouse and human proteins ...
-
No products found
because this supplier's products are not listed.
Yu Rebecca Miao, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... incubated at 37 °C for 30min and column purified using the RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
O Bogen, et al.,
bioRxiv - Neuroscience 2024
Quote:
... psi ε receptor for activated C kinase (ψεRACK) (27) was purchased from Biomatik (Wilmington, DE, USA), and the proinflammatory cytokine prostaglandin-E2 (PGE2 ...
-
Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
Ask
Annika Müller-Eigner, et al.,
bioRxiv - Physiology 2021
Quote:
... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
No products found
because this supplier's products are not listed.
Sanduni I. Fernando, et al.,
bioRxiv - Biophysics 2023
Quote:
U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
-
No products found
because this supplier's products are not listed.
Jasmine George, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... purified human FXR1-GST protein (5 mg/ml) (Novus Biologicals, USA) and purified human GST protein (5 mg/ml ...
-
No products found
because this supplier's products are not listed.
Jessica Audrey Lee, et al.,
bioRxiv - Microbiology 2019
Quote:
... Time-lapse imaging was performed under ambient conditions (regulated at 26 °C ± 1 °C) with a 5-minute period using automated routines (Molecular Devices, Methamorph). All reported experiments were performed once.
-
No products found
because this supplier's products are not listed.
Dian Anggraini, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and maintained in an environmental chamber (Olympus MIIBC; 37 °C, 5% CO2). The fs laser (Spectra-Physics ...
-
No products found
because this supplier's products are not listed.
Florian Wiede, et al.,
bioRxiv - Immunology 2019
Quote:
... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
-
No products found
because this supplier's products are not listed.
Shiyou Zhu, et al.,
bioRxiv - Microbiology 2021
Quote:
Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
-
No products found
because this supplier's products are not listed.
Anna Pepe, et al.,
bioRxiv - Cell Biology 2021
Quote:
Human neuroblastoma (SH-SY5Y) cells were cultured at 37 °C in 5% CO2 in RPMI-1640 (Euroclone), plus 10% fetal bovine serum and 1% penicillin/streptomycin ...
-
No products found
because this supplier's products are not listed.
Swati Gupta, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
-
No products found
because this supplier's products are not listed.
Gregory E. Miner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... at 37 °C and 5% CO2 (Carl Zeiss, Oberkochen, Germany). For movies S1-S4 ...
-
No products found
because this supplier's products are not listed.
Evan R. Buechel, Heather W. Pinkett,
bioRxiv - Biochemistry 2023
Quote:
... then equilibrated for 5 minutes at 18°C in a Spark (Tecan) plate reader prior to fluorescence polarization measurements ...
-
No products found
because this supplier's products are not listed.
Robert Düster, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and the thermal stability was monitored from 20°C to 90°C at a heating rate of 1.5°C/min using the PR.ThermControl software (NanoTemper).
-
No products found
because this supplier's products are not listed.
Aslan Kamil Mahmut, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and kept at 37°C with 5% CO2 in an incubator (New Brunswick Galaxy 170 S ...
-
No products found
because this supplier's products are not listed.
Alessandra Gallinaro, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
-
No products found
because this supplier's products are not listed.
Quentin S. Fischer, et al.,
bioRxiv - Neuroscience 2023
Quote:
... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Arnaud Rondelet, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and derivatives were grown at 5% CO2 and 37 °C in DMEM (PAN Biotech) supplemented with 10% filtered Fetal Bovine Serum (FBS ...
-
No products found
because this supplier's products are not listed.
Kim T Melville, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein fluorescence was measured at 25 °C on a CLARIOstar multimode plate reader (BMG Labtech), with excitation at 295 ± 10 nm ...
-
No products found
because this supplier's products are not listed.
Selene García-Hernández, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
-
No products found
because this supplier's products are not listed.
Suzanne M. Castenmiller, et al.,
bioRxiv - Immunology 2024
Quote:
1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
-
No products found
because this supplier's products are not listed.
Shaohe Wang, Kazue Matsumoto, Kenneth M. Yamada,
bioRxiv - Developmental Biology 2020
Quote:
... spheroids were incubated in 40 µg/mL Atto647N-fibronectin (see Protein labeling) overnight at 37°C with 5% CO2 and transferred into a 4-well 35 mm dish (ibidi, 80466), each well of which contained 2 or 3 spheroids in 100 µL spheroid culture medium with 10 µg/mL Atto647N-fibronectin ...
-
No products found
because this supplier's products are not listed.
Pablo Rougerie, Dianne Cox,
bioRxiv - Cell Biology 2020
Quote:
... The gel was then immediately rinsed with distilled water and incubated overnight at 4°C with a 1mg/mL solution of human whole IgG (CellSciences) or human Fab fragment (Rockland) in 50mM HEPES pH 8 ...
-
No products found
because this supplier's products are not listed.
Victoria L. Castro, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific ...
-
Cat# HY-140336,
inquire
Ask
Miriam Pagin, et al.,
bioRxiv - Genetics 2021
Quote:
... c-Fos/activator protein (AP-1) inhibitor (MedChemExpress), were dissolved in DMSO according to manufacturer’s instructions to obtain the 5 mM ...
-
No products found
because this supplier's products are not listed.
Frederic Fiore, et al.,
bioRxiv - Neuroscience 2022
Quote:
... GABAB receptor antagonist – CGP55845 (5 μM; HelloBio HB0960); adrenergic α1A receptor antagonist – RS17053 (40 μM ...
-
No products found
because this supplier's products are not listed.
Amy Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... for 5 minutes at 125°C in a decloaking chamber (Biocare Medical), washed with 3X with 0.1% Tween-20 (Sigma ...
-
No products found
because this supplier's products are not listed.
Franziska Jäger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
No products found
because this supplier's products are not listed.
Brogan Ashley, et al.,
bioRxiv - Physiology 2021
Quote:
... or 2 μM Receptor Associated Protein (RAP; Innovative Research, USA), which block pinocytosis ...
-
No products found
because this supplier's products are not listed.
Camilla Thorlaksen, et al.,
bioRxiv - Immunology 2022
Quote:
... Samples were stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany), but were equilibrated to room temperature before injections ...
-
No products found
because this supplier's products are not listed.
Bonnie A Thiel, et al.,
bioRxiv - Immunology 2023
Quote:
... Samples were heated to 95°C for 5 minutes in Laemmli buffer (Alfa Aesar), separated on a Novex 4-20% Tris-glycine gel (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
-
No products found
because this supplier's products are not listed.
Mayumi K. Holly, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (MyBioSource). The optical density was read at 450 nm on an EnVision plate reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Sun, et al.,
bioRxiv - Biophysics 2020
Quote:
Plasmids encoding C-terminal eGFP-labeled full-length human LIM proteins were purchased from GeneCopoeia in the m98 vector ...
-
No products found
because this supplier's products are not listed.
Ya-Juan Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or GST-tagged human GABAA receptor β2 subunit protein (GST-β2) (Abnova, catalog #: H00002561-P01) was mixed with 4 μg of recombinant His-tagged human Hsp47 protein (Novus ...
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2023
Quote:
... Avi-tagged Env proteins at 25 µM were dialyzed into Tris pH8 and incubated for 5 hours with mild agitation at 30 °C using the BirA biotin-protein ligase reaction kit (Avidity LLC), then reconcentrated prior to size-exclusion chromatography ...
-
No products found
because this supplier's products are not listed.
Ying Fu, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... After incubation ACE2 with a C-terminal His and AviTag (ACE2-Avi, human ACE2 residues 18-740) (ACROBiosystems, Newark, DE) was added to a final concentration of 4 nM and the resulting mixture was incubated at 25°C for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Wentao Dong, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Media containing γ-retroviruses was harvested after 36-48 h incubation at 37 °C and 5% CO2 and was passed through 0.45 µm filters (Pall). The retentate (∼10 mL ...
-
No products found
because this supplier's products are not listed.
Matthew J. Jansen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The bowel was removed and placed in warmed (37°C), oxygenated (95% O2, 5% CO2) Krebs-Ringers solution in a horizontal organ bath (Hugo Sachs Elektronik, Harvard Apparatus)35 ...