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Citations for Sarstedt :
1 - 50 of 122 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Samples were stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany), but were equilibrated to room temperature before injections ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 min at 4°C in Micro tube 1.1ml Z-Gel (Sarstedt, Germany). Samples were kept on ice at all times ...
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bioRxiv - Immunology 2022Quote: ... the unstressed sample was prepared once weekly and stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany). The sample was used for two rounds of injections and hereafter discarded.
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated sample was centrifuged (15000 x g, 30 min, 4 °C) and the supernatants were transferred into fresh DNA-low binding tubes (Sarstedt, Cat.-No. 72.706.700). The protein concentration was measured using Bradford assay (Roti®-Quant ...
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bioRxiv - Microbiology 2022Quote: ... GG770 and LL1021 cells were grown aerobically at 37 °C under agitation in 5 ml of M9 minimal medium in 50 ml conical polypropylene tubes (Sarstedt) with an inclination of 90° ...
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bioRxiv - Neuroscience 2023Quote: We collected blood from ad-libitum fed 9 months-old mice through cardiac puncture and centrifugation at 2000 g for 5 minutes at 4 C° in heparinised tubes (Microvette CB 300; Sarstedt, Leicester, UK) for plasma collection ...
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bioRxiv - Biochemistry 2023Quote: ... and manufacturer C (SafeSeal reaction tube no. 72.706 and SafeSeal reaction tube, Low protein-binding no. 72.706.600, Sarstedt AG & Co. KG, Nümbrecht, Germany). Further ...
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bioRxiv - Microbiology 2022Quote: ... 22 or 25 °C was split into two new 50 mL flasks (Sarstedt Germany) with one used as a control and the other flask supplemented with the ribosome blocker lincomycin (0.5 mg mL−1 final concentration ...
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bioRxiv - Molecular Biology 2023Quote: Atu strains were precultured overnight at 28°C in 14 ml polypropylene tubes (Sarstedt) in a rotary shaker ...
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bioRxiv - Cancer Biology 2023Quote: Biopsies were removed from −80°C and placed immediately into 2 mL microtubes (Sarstedt, Nuembrecht, Germany) filled to ∼1/3 volume with 2 and 0.1 mm diameter zirconia beads (Biospec ...
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bioRxiv - Microbiology 2020Quote: ... The strains were incubated at 25 °C for 24 hours in 25 cm2 cell culture flasks (Sarstedt). The strains were then diluted 1:10 in Fraquil and 1 ml were distributed in 13-ml tubes (Sarstedt ...
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bioRxiv - Bioengineering 2020Quote: ... 3D micromass pellets were formed by centrifuging 5×105 cells at 300 x g in 1.5 mL conical tubes (Sarstedt) and cultured for two weeks in chondrogenic serum-free medium consisting of DMEM containing 1 mM sodium pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... and inoculated in 500 µL MSgg medium inside an 8-well tissue culture chamber at 30°C (Sarstedt; width ...
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bioRxiv - Neuroscience 2023Quote: ... the organoid pieces were digested in 2 ml of CellLive™ Tissue Dissociation Solution (#1190062, Singleron Biotechnologies,) at 37°C for 15 minutes in a 15-ml centrifuge tube (#62.5544.003, Sarstedt,), with continuous agitation on a thermal shaker ...
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bioRxiv - Plant Biology 2024Quote: ... 4°C) and the supernatant was filtrated through a 0.2 µm syringe filter (cat-no. 83.1826.001; Sarstedt, www.sarstedt.com). The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: ... The plates were incubated for 16 h at 37 °C (horizontally shaking at 180 rpm) and rearranged into one 96-well plate (MegaBlock 96 Well, 2.2 ml, Sarstedt). 110 Ml/well of bacteria was used to create glycerol stock plate (Round 96 Well Storage Plates ...
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bioRxiv - Microbiology 2022Quote: ... Viral culture supernatant was harvested 96 hrs post-infection and clarified then stored at −80 °C in 500 μL aliquots in 2mL cryovials (72.694.406, Sarstedt, Inc.) until required ...
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bioRxiv - Microbiology 2023Quote: ... The cell debris was removed by centrifugation at 12,000 × g for 15 min at 4°C and the supernatant filtered through a 0.45 μm pore size Filtropur S membrane (Sarstedt). The cleared extract was directly loaded onto a HisTrap FF crude column (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: Barcoded Asaia bacteria were grown overnight at 30°C to early log phase (OD600 0.5-0.8) in a deep-well plate (Sarstedt, Nümbrecht, Germany) in 300 μl GLY medium supplemented with 100 μg/ml kanamycin per well ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were recovered for 5 hours at 30°C in YP medium with 2% maltose and then spread onto 50 petri dishes (150×20 mm, Sarstedt) with YNB + CSM Ura− with 2% maltose supplemented with G418 at 0.2 mg mL−1 ...
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bioRxiv - Microbiology 2019Quote: ... Cultures were inoculated to an initial OD600 of 0.05 into fresh medium from overnight liquid cultures grown in LB in 18mm borosilicate culture tubes at 37°C on a roller drum in 15 mL conical screw-cap tubes (Sarstedt). Tubes were placed in 2.5 liter AnaeroPack System rectangular jars (Mitsubishi Gas Chemical Co. ...
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bioRxiv - Neuroscience 2023Quote: ... Samples of each peptide and the peptide mix were incubated at 37 °C in sealed 2 ml non-treated plastic cryotubes (Sarstedt) for 24 hours and amyloid content was determined by ThT fluorescence and TEM (data not shown).
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bioRxiv - Physiology 2021Quote: Adult flies of the desired genotype were collected and aged for 6 days at 25°C and transferred into 1% agar (in dH2O) contained in 12 mL vials (SARSTEDT, 58.487). Dead animals were counted in 15 ...
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bioRxiv - Microbiology 2019Quote: The level of pyoverdine and pyocyanine production was analyzed on a 72h-biofilm formed on Nephrophane or selected PAO1 mutants cultured (48 h, 37°C) in TSB medium (bioMerieux, France) in a 24-well polystyrene plate (Sarstedt, Germany) (Danis-Wlodarczyk et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... D and E) or as catheter urine from a fresh collection bag (timepoint B and C) into standard urine sample cups (Sarstedt, Germany). Preservatives and protease inhibitors were added to the final concentrations 6,6mM NaN3 ...
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bioRxiv - Microbiology 2023Quote: ... where a large volume of passage one stock was made and stored at -80°C in 500 μL aliquots in 2 ml cryovials (72.694.406, Sarstedt Inc. (Nümbrecht, Germany)) until required ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial growth was determined after 24 h of incubation (37°C, 200 rpm) by measuring at OD600 using standard photometer cuvettes (Sarstedt, Germany). An identical protocol was applied for the 20 clinical NDM-producing isolates with the only modification of using a fixed concentration of 4 µg/ml of aztreonam.
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bioRxiv - Microbiology 2023Quote: ... Parents were instructed to collect infant faeces from diapers and freeze them immediately at –20◦C in their home freezer inside a cool transport container (Sarstedt, Hilden, Germany). Samples were transported to the laboratory ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: ... the organoid pieces were digested in 2 ml of CellLive™ Tissue Dissociation Solution (Singleron Biotechnologies, #1190062) at 37°C for 15 minutes in a 15-ml centrifuge tube (Sarstedt, #62.5544.003), with continuous agitation on a thermal shaker ...
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bioRxiv - Microbiology 2019Quote: ... and used to inoculate 200 µL of M9 medium supplemented with the desired C-source in a 96-well microtiter plate (Falcon, product no. 353047 or Sarstedt, product no. 83.3924) and incubated at 30°C and 250 rpm in a microplate reader (Xenius ...
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bioRxiv - Genetics 2022Quote: ... Blood was collected by venous puncture or cardiac puncture at the time of sacrifice and centrifuged at 2000 g for 15 minutes at 4 °C in heparin-coated tubes (Sarstedt AG, Nürnbrecht, Germany) to separate plasma from cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... colonies were kept in square plaster-bottom nests (100 x 100 x 20 mm, Sarstedt, Germany) and held in climate chambers under a 12h/12h light/dark cycle and a 28°C/22°C temperature cycle ...
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bioRxiv - Neuroscience 2019Quote: ... 35 x 10 mm (SARSTEDT #83.3900.500), before injection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Colonies were kept in square plaster-bottom nests (100 mm x 100 mm x 20 mm, Sarstedt, Germany) with plastic inserts containing three chambers covered in dark foil ...
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bioRxiv - Plant Biology 2023Quote: Ten days after sowing plants cultured on vertical square Petri dishes (10 x 10 x 2 cm, Sarstedt, Ref. 82.9923.422) were placed in a vertical position in sealed plastic boxes containing fungal cultures ...
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bioRxiv - Cell Biology 2022Quote: ... Ovarioles were transferred to X-well chambers (Sarstedt 94.6190.802) equipped with 200 µl live imaging-medium (Schneideŕs Drosophila medium containing 15% FCS and 0.2 mg/ml insulin) ...
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bioRxiv - Bioengineering 2024Quote: ... plastic tissue culture dishes (Sarstedt, 100 x 20mm, 83.3902) were coated with collagen (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: 8 x 104 cells were seeded on 96 well plates (Sarstedt, 83.3925) and treated with various doses of N3a after 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocytes were seeded into 6-well plates (Sarstedt, 83.3920) at 100,000 cells/well and either live-imaged or serially imaged as indicated ...
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bioRxiv - Bioengineering 2022Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany). The 1 mg/mL stock solutions were further diluted to working solutions consisting of 10 ng/μL human or bovine insulin ...
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bioRxiv - Biochemistry 2023Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany).
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bioRxiv - Cell Biology 2022Quote: ... the pTr1 cells (5×105 cells/5 ml/flask) were seeded in T25 flasks (83.3910.002; Sarstedt) in CM ...
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bioRxiv - Immunology 2023Quote: ... Cells (1 x 105/ml) were plated in a 48-well plate (Sarstedt, 83.3923) precoated overnight at 4 °C with 1 µg/ml anti-CD3χ and 3 µg/ml anti-CD28 ...
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bioRxiv - Plant Biology 2023Quote: ... aurantiogriseum was cultured in small Petri dishes (35 x 10 mm, Sarstedt, Ref. 82.1135.500) containing solid MS medium supplemented with 90 mM sucrose ...
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bioRxiv - Immunology 2023Quote: ... 6 x 106 lung cells were plated in a 6-well plate (Sarstedt, 83.3920) and restimulated with 50 ng/ml Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Plant Biology 2020Quote: ... the medium was poured into petri dishes (92 x 16 mm, Sarstedt AG & Co. KG). All steps were carried out in a laminar flow hood with sterile equipment ...
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bioRxiv - Immunology 2023Quote: ... 500µl of 1 x 106 mast cells/ml were added to 24 well plates (Sarstedt) and infected with 25µl inoculum ...
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bioRxiv - Biochemistry 2022Quote: ... The urea-denatured LipA-FM was diluted with the TGCG buffer from 150 μM stock solution to 1.6 μM and kept on ice protein in reaction tubes (Low Protein Binding, Sarstedt). The chaperones were transferred to 1.5 mL polypropylene reaction tubes containing 20 mM Tris-HCl pH 8.0 and varying NaCl concentrations ...