-
No products found
because this supplier's products are not listed.
Dorothy Y. Zhao, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and digested overnight at 37 °C with 1 µg Lys-C (Labchem-wako) and 2µg trypsin (Promega) ...
-
No products found
because this supplier's products are not listed.
Lindsay Smith, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... zebrafish were incubated for 5 min at 28.5°C with optovin 6b8 (ID 5705191l; ChemBridge), an optovin analog (Kokel et al. ...
-
No products found
because this supplier's products are not listed.
Milena Petkova, et al.,
bioRxiv - Cell Biology 2022
Quote:
Mouse Vascular Endothelial Cell Growth Factor C (VEGF-C) ELISA Kit from CUSABIO (CSB-E07361m) was used for detection of VEGF-C protein concentration ...
-
No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Elizabeth B. Draganova, et al.,
bioRxiv - Biophysics 2023
Quote:
... Reactions incubated for 5 min at 20 °C before imaging in 96-well chambered coverglass (Brooks Life Science Systems). Samples were imaged using a Nikon A1R Confocal Microscope with a 60x oil immersion lens at the Imaging and Cell Analysis Core Facility at Tufts University School of Medicine ...
-
No products found
because this supplier's products are not listed.
Xiaoke Chi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Membranes were pelleted by centrifugation at 13000g for 10 min and cytochrome c release was analyzed by immunoblotting using a sheep anti-cytochrome c antibody (Capralogics). Mitochondria from embryonic mouse brains for BH3 profiling experiments were prepared from ~20 mouse embryos ...
-
No products found
because this supplier's products are not listed.
Mia P. Sams, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Samples were denatured at 100°C and separated on 10% SDS-PAGE gels with BLUeye Prestained Protein Ladder (FroggaBio, Concord ON). Protein was then semi-dry transferred to a PVDF membrane and incubated in Li-Cor Intercept® Blocking Buffer (Cedarlane ...
-
No products found
because this supplier's products are not listed.
Jason Yang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... supplemented with 100 μg/mL carbenicillin (AG Scientific C-1385) in 96-well polypropylene plates overnight at 37 °C ...
-
No products found
because this supplier's products are not listed.
Masato Morita, Reo Higashi, Shin-ya Kawaguchi,
bioRxiv - Neuroscience 2023
Quote:
HEK293T cells were cultured at 37 °C in humidified air containing 5 % CO2 in DMEM (Nakalai Tesque, Kyoto, Japan) supplemented with 10 % (v/v) FBS (NICHIREI BIOSCIENCES, Tokyo, Japan) and penicillin (100 U/mL)-streptomycin (0.1 mg/mL ...
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No products found
because this supplier's products are not listed.
Irene Pereira de Sousa, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 50 mg of dried MSPs were degassed for 1 h at 100 °C with nitrogen and then analyzed by nitrogen sorption at −196 °C (TriStar 3000, Micromeritics, Norcross, GA, USA). The morphology of MSPs was assessed by SEM (Quanta 200F ...
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No products found
because this supplier's products are not listed.
Aanandita Kothurkar, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... and frozen at −80°C. SuperFrost™ Plus Adhesion Microscope Slides (Epredia, Cat. No. J1800AMNZ) were coated evenly with 5 μL chrome alum gelatin (Newcomer Supply, Part# 1033C) and dried in an incubator at 60°C for one hour ...
-
No products found
because this supplier's products are not listed.
Kévin Leguay, et al.,
bioRxiv - Cell Biology 2020
Quote:
Coelenterazine 400a (Deep Blue C) and methoxy e-CTZ (Prolume Purple) were purchased from NanoLight Technology (#340 and #369 ...
-
No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
-
No products found
because this supplier's products are not listed.
Erika Riederer, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1 µM valinomycin at 30 °C using 100 nM of 14C Asp (Moravek Biochemicals) as previously described.(33 ...
-
No products found
because this supplier's products are not listed.
Kelsey E. Zuk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The tissue was superfused with oxygenated ACSF at 35°C via a peristaltic pump (Gilson) at a rate of 3-3.5 ml/min.
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No products found
because this supplier's products are not listed.
Kelly L. Buchanan, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or the sweet taste receptor inhibitor (T1R2/3) gurmarin (Peptides International) were dissolved into 1M sucrose ...
-
No products found
because this supplier's products are not listed.
Bence Daniel, et al.,
bioRxiv - Genomics 2024
Quote:
... Bone marrow-derived macrophages (5 x 106) were double crosslinked by 50mM DSG (disuccinimidyl glutarate, #C1104 - ProteoChem) for 30-minutes followed by 10-minutes of 1% formaldehyde ...
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No products found
because this supplier's products are not listed.
Elizabeth M. Garrett, et al.,
bioRxiv - Microbiology 2019
Quote:
... followed by a 5-nm thickness platinum sputter in a Hummer X sputter coater (Anatech USA, Union City, CA). Images were taken with a working distance of 5 mm and a 130-μm aperture using a Zeiss Supra 25 field emission scanning electron microscope (FESEM ...
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No products found
because this supplier's products are not listed.
Benjamin L. Woods, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells were grown to mid-log phase at 24°C in Complete Synthetic Medium (CSM, Sunrise Science Products) supplemented with 0.67% yeast nitrogen base ...
-
No products found
because this supplier's products are not listed.
Joakim Karlsson, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were surface stained for 45 min in 37°C using Melanoma Dextramer Collection 1 kit from Immudex. Dead cells were excluded from the analysis using Live/Dead Aqua (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Victor H. Gonzalez, et al.,
bioRxiv - Physiology 2020
Quote:
... We used a water bath with a volume of 12 L controlled by a thermostat (18–100 °C; Bellco Sci-Era Hot Shaker ...
-
No products found
because this supplier's products are not listed.
Abigail E. Powell, et al.,
bioRxiv - Immunology 2020
Quote:
... plates were washed 3X with PBST and blocked overnight at 4 °C with ChonBlock Blocking/Dilution ELISA Buffer (Chondrex). ChonBlock was removed manually and plates were washed 3X with PBST ...
-
No products found
because this supplier's products are not listed.
Marissa M. Gaskill, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... attP40{nos-Cas9}/CyO for the N-terminal line and y1 M{vas-Cas9.RFP-}ZH-2A w1118 (BDSC#55821) for the C-terminal line by BestGene Inc ...
-
No products found
because this supplier's products are not listed.
J.T. Stieglitz, K.A. Potts, J.A. Van Deventer,
bioRxiv - Synthetic Biology 2021
Quote:
... samples were first labeled in 50 µL room-temperature PBSA with 1:500 dilutions of chicken anti-c-Myc (Exalpha Biological ...
-
No products found
because this supplier's products are not listed.
Cornelia Blume, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
-
No products found
because this supplier's products are not listed.
Jugal Mohapatra, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and heated to 90 °C for 2 min while bubbling with nitrogen gas in N,N-dimethylformamide (DMF, Oakwood Chemical). Fmoc deprotection was carried out with 20% piperidine (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Alyssa F. Pybus, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were incubated at 4°C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200 ...
-
No products found
because this supplier's products are not listed.
Huibao Feng, et al.,
bioRxiv - Genetics 2022
Quote:
... for 1 h at 37°C for recovery and then plated onto 37 Φ150 LB agar plates containing kanamycin with an EasySpiral Pro (Interscience). Generally ...
-
No products found
because this supplier's products are not listed.
Henriette Frikke-Schmidt, et al.,
bioRxiv - Physiology 2020
Quote:
... The plates for the islets were placed on a mat heated at 37 °C on a H101A ProScan motorized stage (Prior Scientific Instruments Ltd ...
-
No products found
because this supplier's products are not listed.
Cameron Durfee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... They were then baked at 65°C for 20 minutes to deparaffinize and then rehydrated with three consecutive washes in CitriSolv (Decon Labs) for 5 min each followed by graded alcohols as above ...
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No products found
because this supplier's products are not listed.
M. Martin VandenBroek, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Gradients were centrifuged at 40,000 rpm for 1.5 hours at 4°C and were fractionated using a BioComp fractionator (BioComp Instruments, Fredericton NB) into 800 μL fractions that were divided equally for the isolation of either protein or RNA ...
-
No products found
because this supplier's products are not listed.
Philippe Dehio, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The slides were first plasma cleaned and PEG-coated at 4 °C overnight with 200µg/ml poly(L-lysine)-PEG (SuSoS, PLL(20)-g[3.5]-PEG(2) ...
-
No products found
because this supplier's products are not listed.
Sarah Mondoloni, et al.,
bioRxiv - Neuroscience 2022
Quote:
... slices (250 μm thick) were cut in SB-aCSF at 0-4°C using a Compresstome slicer (VF-200, Precisionary Instruments Inc.). Slices were then transferred to the same solution at 35°C for 10 min ...
-
No products found
because this supplier's products are not listed.
Mobashar Hussain Urf Turabe Fazil, et al.,
bioRxiv - Immunology 2021
Quote:
Real-time trans-well migration of T-cells was monitored at 37°C using the CIM-Plate 16 and xCELLigence impedance-based detection system (ACEA biosciences, Singapore) to evaluate transmigration of T-cells towards the chemoattractant SDF-1α (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Mathew Miller, et al.,
bioRxiv - Molecular Biology 2022
Quote:
MULTI-ARRAY Standard 96-well plates (Meso Scale Diagnostics, Rockville, Maryland) were coated overnight at 4°C with K1 anti-dsRNA mouse monoclonal antibody (SCICONS, Budapest, Hungary). Plates were blocked using 5% MSD Blocker A (Meso Scale ...
-
No products found
because this supplier's products are not listed.
Ingrid Lekk, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was used for block and for over-night primary antibody incubation at 4°C with the following antibodies: rabbit anti-GFP (dilution 1:1000, Torrey Pines Biolabs, Cat# TP401), mouse anti-acetylated tubulin (dilution 1:250 ...
-
No products found
because this supplier's products are not listed.
Chao Ye, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Genomic DNA extraction was done by either boiling cells in 25 μL of 20 mM NaOH at 95 °C for 15 min or by using the Direct DNA Extraction Kit (Bacteria) (Norgen Biotek, Thorold, ON, Canada) following the manufacturer’s manual.
-
No products found
because this supplier's products are not listed.
Angela Kim, et al.,
bioRxiv - Cell Biology 2021
Quote:
... was from Tocris (Bio-Techne Ltd, UK). The glucagon receptor antagonist LY2409021 (adomeglivant; Kazda, et al. (30)) was from MedKoo Biosciences (USA).
-
No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... panBAG-1 (human, rabbit monoclonal, RM356, RevMAb), panBAG-1 (mouse ...
-
No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
-
No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human neutrophils were freshly isolated using Polymorphprep (Alere technologies) per manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Valeria Taliani, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... resuspended with 5 μg of MATR3 (Supplementary File 5) or IgG antibodies (Immunoreagents Inc.) and incubated for 2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Johanna F. Dekkers, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 5 μM Y-27632 (Abmole), 5 nM Heregulin β-1 (Peprotech) ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the human multimeric vitronectin solution (Molecular Innovations Inc, HVN-U) was adjusted to a concentration of 1 mg/mL and then its buffer was exchanged to 0.1 M sodium bicarbonate using a Zeba spin desalting column (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Grigoria Spanou, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli was grown on Chromogenic Tryptone Bile X-glucuronide (TBX) Agar (Neogen. USA) at 37°C for 24h ...
-
No products found
because this supplier's products are not listed.
Aleksandra A. Petelski, et al.,
bioRxiv - Bioengineering 2021
Quote:
... myFuge 5 (MTC Bio; cat. no: C2595).
-
No products found
because this supplier's products are not listed.
Jan Christoph Thiele, et al.,
bioRxiv - Biophysics 2020
Quote:
... was incubated with a 10 x excess of Tetrazine-ATTO655 (ATTO-TEC, AD 655-2505) at RT and purified again after 15 min.
-
No products found
because this supplier's products are not listed.
Zelin Liu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... second-strand cDNAs were synthesized using P2-T24 (5’-ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTT-3’) by I-5 High-Fidelity DNA polymerase (MCLAB) at 98°C for 2 min ...
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Recombinant Antigen
Cat# REC31700-100,
100µg USD $488.0
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Alexander A. Lehmann, et al.,
bioRxiv - Immunology 2020
Quote:
... truncated Spike protein (S1 domain) (The Native Antigen Company, Oxford, UK) or receptor binding domain (RBD ...
-
No products found
because this supplier's products are not listed.
Sumit J. Bandekar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... ADGRL3 + GFP and TEN2 + dsRed and 5 μL LipoD293T (SL100668; SignaGen Laboratories). Two days after transfection ...