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Citations for Sartorius :
1 - 50 of 344 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were grown at 37°C and 5% CO2 in an Incucyte Zoom (Sartorius) where cell death was monitored hourly.
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bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were grown at 37°C and 5% CO2 in an Incucyte Zoom (Sartorius). Cell death was monitored hourly.
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bioRxiv - Biochemistry 2023Quote: ... C-terminally biotinylated SKP1-FBXO22 fusion proteins (WT or C326A) were immobilized onto SA biosensors (Sartorius) to a response level of 1 nm ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Cell Biology 2019Quote: Live-cell imaging was performed at 37°C under humidified 5% CO2 atmosphere using the IncuCyte Zoom system (Sartorius). Phase and fluorescence images were also acquired using Leica DiM8 and Zeiss LSM710 microscopes ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C in Vivaspin20 columns (Cat.#VS2032,Sartorius) and stored at –80°C.
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Immunology 2023Quote: ... The cocultures were incubated for 48 hours in a humidified incubator (37 °C, 5% CO2) equipped with an Incucyte (Sartorius) for continuous monitoring of cell proliferation and target cell killing ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...
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bioRxiv - Genomics 2024Quote: The human T lymphoblast Sup T1 cell line was cultured at 37°C under a 95% air and 5% CO2 atmosphere in Roswell Park Memorial Institute medium (RPMI 1640 medium; Sartorius #01-100-1A) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were then incubated at 37° C in IncuCyte S3 (Sartorius) and real-time live-cell proliferation analysis was performed at regular intervals of 4 hours for 12 days with phase contrast image acquisition mode using Incucyte S3 software ...
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bioRxiv - Immunology 2024Quote: ... GST-c-SLFN11 was immobilized onto Anti-GST Biosensor tips (Sartorius) in BLI buffer by dipping the biosensors into wells containing GST-c-SLFN11 for 300 s ...
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bioRxiv - Synthetic Biology 2023Quote: ... instrument at 30 °C and 1000 rpm with AHC biosensors (Sartorius). Various F-FGF21 or L-39F7 proteins were diluted to 50 nM in the running buffer and captured by the AHC biosensor ...
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bioRxiv - Biophysics 2024Quote: SPR measurements were performed at 25 °C using a Pioneer FE instrument (Sartorius). Recombinant CaMKIIα WT hub ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and the supernatant was passed through a 0.45 µm syringe filter (Sartorius). StrepII-CERT was captured using 1 mL StrepTrap HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 °C on Octet K2 (Sartorius AG). A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1 ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37°C in the IncuCyte S3 LiveCell Analysis System (Sartorius) and five images of each well were recorded every 3 hours and analyzed with the IncuCyte image analysis software to determine the number of infected cells becoming fluorescent overtime.
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bioRxiv - Biochemistry 2024Quote: Analysis of binding kinetics was done at 23 °C using Octet K2 (Sartorius AG), as previously described (61) ...
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bioRxiv - Microbiology 2019Quote: ... to an aerated cooling bioreactor set at 15 °C (Sartorius Stedim Biostat Q Plus, Germany). On the other side ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated by centrifugation at 4,000xg at 4°C through VivaSpin 10 kDa MWCO concentrators (Sartorius), aliquotted ...
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
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bioRxiv - Synthetic Biology 2022Quote: ... Co-cultures were incubated at 37°C and imaged with an Incucyte S3 Live-Cell Analysis System (Sartorius) for approximately 72 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated for 16 h at 37 °C following removal of RiGH26 using a Vivaspin 20 filtration unit (10.000 MWCO PES, Sartorius) and carbohydrate lyophilization on an ALPHA 2-4 LD Plus freeze dryer (Christ ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius), before being washed again and resuspended in complete RPMI medium ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 3 mL of Glutathione Sepharose 4B resin (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). For purification of ScaC and ScaC-SNAP ...
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bioRxiv - Biochemistry 2023Quote: ... The experiments were conducted at 20°C. The resulting data were processed using Octet Analysis Studio (ver. 13.0)(Sartorius) and reference biosensor (loaded with GST-only ...