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Citations for Worthington Biochemical :
1 - 50 of 253 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2019Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Genomics 2019Quote: Nuclei from 5×105 cells were aliquoted into microcentrifuge tubes and incubated at 37°C for 5 min with varying amounts of DNase (0 to 75 U; Worthington, LS006344). Digestion was stopped by addition of an equal volume of termination buffer [10 mM Tris (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated in 37°C Trypsin-DNase (1g/L DNase-Worthington Biochemical- ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Genetics 2023Quote: ... To digest the polypeptide 10 µL of Glu C enzyme (Worthington) at 100 µg/mL was added and the samples digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... at 4°C overnight and then digested with collagenase type II (Worthington). The cardiomyocyte fraction was collected after differential plating for 70 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... muscle fibres were digested with collagenase (CLSAFA, Worthington; 1 h; 37 °C), followed by 100 μm and 40 μm cell filtration steps ...
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bioRxiv - Physiology 2022Quote: ... at 37 °C with 229 U/ml collagenase type II (Worthington Biochemical Corporation), and washed in Tyrode solution for enzyme removal ...
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bioRxiv - Cell Biology 2020Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Immunology 2021Quote: ... Kidneys were digested at 37°C in 1 mg/ml collagenase IV (Worthington) in complete RPMI for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... after digestion by papain and DNase (Worthington for 10 min at 37°C). Transfections were performed using the Amaxa nucleofector system following the manufacturer’s manual ...
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bioRxiv - Neuroscience 2020Quote: ... retinae were dissociated at 37°C for 30min with papain (LK003160, Worthington, Lakewood, NJ). The same volume of Neurobasal medium (Thermo Fischer ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003160, 1 bx, $265.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... and digested for 16 hr at 37°C with sequencing grade trypsin (Worthington Biochemical) in a 1:100 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: Tissues were digested enzymatically for 30 minutes at 37°C with papain (Worthington, LS003126) 20 units/mL in Ames ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated at 37°C with OPC papain solution (Worthington Cat#LS003124) for 20 minutes with constant inversion to prevent tissue aggregation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003124, 25 mg, $40.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... and enzymatically digested at 37 °C first using 1 mg/ml Collagenase IV (Worthington, US) in L15 for 25 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and digested overnight at 37 °C with 1 µg of trypsin (Worthington, LS003740 TPCK-treated). Samples were then acidified with formic acid (1 % final concentration).
Product cited:
A chromatographically purified, diafiltered, lyophilized powder that has been treated with...Cat# LS003740, 100 mg, $112.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... followed by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Biochemistry 2023Quote: ... the particles were incubated at 37°C with 1 μg/μL trypsin TPCK (Worthington-Biochem) for 2 h to induce the proteolysis of BSA and measure in the spectrofluorometer ...
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bioRxiv - Physiology 2024Quote: ... immediately frozen in a dry shipper containing liquid nitrogen (∼-190°C, ©CX100 Worthington Industries) on-board and later stored at -80 °C at the lab until proteomics analyses ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then by collagenase type II for ∼16 hours at 37°C (583 Units/ml Worthington Biochemical Corp. ...
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bioRxiv - Immunology 2021Quote: ... tissues were digested in Collagenase solution for 1 hour at 37°C (Worthington Biochemical, Lakewood, NJ). Cells were washed once and blocked by Fc Block (Clone ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Ganglia were incubated at 37□C for 25 minutes in 1 mg/ml papain (LS003119; Worthington), followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176 ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... Chopped samples were incubated at 37°C for 30 min in collagenase III (3 mg/ml; Worthington). Liver samples were excised and meshed into single-cell suspensions through 70 μm meshes ...
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bioRxiv - Physiology 2021Quote: ... followed by a 15-min incubation at 37°C in type II collagenase (1.0 mg/ml) (Worthington) in Mg-PSS ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated at 37°C for 30 minutes with collagenase type II (8480 U/ml, Worthington Biochemical), elastase (7.5mg/ml ...
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bioRxiv - Immunology 2020Quote: Endoproteinase Gluc-C (Staphyloccoccus aureus Protease V8) and chymotrypsin were obtained from (Worthington Biochemical Corp., Lakewood, USA). The ultrapure milli-Q deionized water (MQ ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was then transferred to 1 mL tubes containing papain solution pre-warmed to 37° C (Worthington Biochemical LS003119 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested at 37°C for 30 min with 1 mg/mL Collagenase I (Worthington Biochemical, LS004194), 3 mg/mL Dispase II (Roche ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... hippocampi were later incubated twice for 15 minutes at 37°C with 100 units of papain (Worthington, NY) in DM and rinsed three times in DM and subsequently three times in plating medium (MEM ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested rotating for 30 minutes at 37°C with 1 mg/mL Collagenase I (Worthington Biochemical LS004194), 3mg/mL Dispase II (Roche 04942078001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask