-
No products found
because this supplier's products are not listed.
Pierre Barraud, et al.,
bioRxiv - Molecular Biology 2019
Quote:
All in vitro NMR spectra of yeast tRNAPhe were measured at either 38°C or 30°C on Bruker AVIII-HD 600 MHz and AVIII-HD 700 MHz spectrometers (equipped with TCI 5-mm cryoprobes) with 5-mm Shigemi tubes ...
-
No products found
because this supplier's products are not listed.
Seong Beom Ahn, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the peptide trap was switched in-line with a cHiPLC C18 column (15 cm x 200μm, 3μm, ChromXP C18-CL, 120 Å, 25°C, SCIEX) and peptides were eluted using a linear 120min gradient from 5 % acetonitrile to 35 % mobile phase B (B ...
-
No products found
because this supplier's products are not listed.
Wenxin Kou, et al.,
bioRxiv - Genetics 2022
Quote:
... were cultured at 37 °C with 5% CO2 in endothelial cells medium (ECM, Cat#1001, ScienCell, USA) with 5% fetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... the DNA template has a 5’ C and the dNTPs included 0.5 μM of fluorescein-12-dGTP (AAT Bioquest) in addition to 50 μM of dATP ...
-
No products found
because this supplier's products are not listed.
Kelsey A. Haugh, et al.,
bioRxiv - Microbiology 2020
Quote:
... or permeabilized with PBS containing 0.2% Triton X-100 (for stains of intracellular proteins) and treated with Fc receptor blocker (Innovex Biosciences), Richmond ...
-
No products found
because this supplier's products are not listed.
Ida Søgaard Larsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... and C-peptide by Mouse C-peptide ELISA kit (Crystal Chem) following the manufacturers’ protocols.
-
No products found
because this supplier's products are not listed.
Selma Belhimeur, et al.,
bioRxiv - Immunology 2022
Quote:
... 95°C for 10 min, 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System ...
-
No products found
because this supplier's products are not listed.
Jun Feng, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... the cell pellets were resuspended into BMM-C and then inoculated into 5 mL BMM or BMM-xyloglucan media (5 g L-1 xyloglucan, Megazyme) to an initial OD600 of 0.05 ...
-
No products found
because this supplier's products are not listed.
Maja C Stöber, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The probes were denatured by incubation at 72 °C for 5 min in a Thermobrite (Abbott) followed by overnight incubation at 37 °C ...
-
No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Quantitative analysis of the CFTR B and C-bands was performed using Compass software (Protein Simple).
-
No products found
because this supplier's products are not listed.
Eliona Tsefou, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
-
No products found
because this supplier's products are not listed.
Dennis M Defoe, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... then incubated overnight at 4°C in combined primary antibodies (chicken anti-green fluorescent protein [GFP]; Aves Labs, Tigard ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
-
No products found
because this supplier's products are not listed.
Shiwei Liu, et al.,
bioRxiv - Genomics 2023
Quote:
... we grew parasites at 37°C in vitro at 3% hematocrit (serotype A positive human erythrocytes, Valley Biomedical, VA or BioIVT, NY) in RPMI 1640 medium (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Samuel R. Witus, et al.,
bioRxiv - Biochemistry 2023
Quote:
... cells were shifted from 37 °C to 16 °C and supplemented with 1 mM Bpa (dissolved in 1M NaOH; Bachem) and 100 µM ZnCl2 ...
-
No products found
because this supplier's products are not listed.
Andrew LaPoint, et al.,
bioRxiv - Physiology 2023
Quote:
... and cholesterol (2%) (HFHF-C, Research Diets, D09100310) diet or a matched sucrose ...
-
No products found
because this supplier's products are not listed.
Donna Ye, et al.,
bioRxiv - Microbiology 2019
Quote:
... Overnight cultures of strains in either Typticase Soy Broth (Difco) or R2B (3-5 mls) were grown at 25°C in a rotating rack (Cole-Parmer). Sterile broth (75-100µl ...
-
No products found
because this supplier's products are not listed.
Hongje Jang, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Cells were cultured on coverglasses in 24 well cell culture dishes at 37°C (5% CO2) in DMEM supplemented with 10% fetal bovine serum (FBS; Atlanta Biological) and 1% penicillin/streptomycin (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Iosifina P. Foskolou, et al.,
bioRxiv - Immunology 2022
Quote:
... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
-
No products found
because this supplier's products are not listed.
Inês Milagre, et al.,
bioRxiv - Cell Biology 2020
Quote:
... guinea-pig polyclonal anti-CENP-C (#PD030, MBL International) at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Huacheng Luo, et al.,
bioRxiv - Genetics 2019
Quote:
... followed by drying at 37°C and Giemsa staining (Amresco)28.
-
No products found
because this supplier's products are not listed.
Nikhila S Tanneti, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Reverse-phase chromatography was performed at 50 °C over a 25 cm IntegraFrit column (IF360–75-50-N-5, New Objective, Woburn, MA) packed in-house with 1.9 μm ReproSil-Pur C18-AQ (Dr ...
-
No products found
because this supplier's products are not listed.
Annemarie Lang, et al.,
bioRxiv - Bioengineering 2024
Quote:
... KS) and moved onto a heating pad (37 °C; Kent Scientific, Torrington, CT). Anesthesia was maintained at ∼1.5–2% with an individual a nose cone ...
-
No products found
because this supplier's products are not listed.
Julie Firmin, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Embryos are placed in pre-equilibrated (at least 4 h at 37°C, 5% O2, 5% CO2) CSCM-C medium (Irvine Scientific) covered with mineral oil (Irvine Scientific ...
-
No products found
because this supplier's products are not listed.
Steven Swendeman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Activated Protein C was obtained from Enzyme Research laboratories. For initial studies ...
-
No products found
because this supplier's products are not listed.
Francisco Santos, et al.,
bioRxiv - Cell Biology 2024
Quote:
... for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech, MB09002) was used ...
-
No products found
because this supplier's products are not listed.
Shawn M. Costello, et al.,
bioRxiv - Biophysics 2021
Quote:
... and packed by hand into a column (2mm ID x 2 cm, IDEX C-130B). Following digestion ...
-
No products found
because this supplier's products are not listed.
Melissa J. Mann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
-
No products found
because this supplier's products are not listed.
Shan Jiang, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The protein standard with the C-terminal FLAG tag (E-PKSH032870.10) was from Biomol. Linear DNA templates were produced by PCR and were PCR purified ...
-
No products found
because this supplier's products are not listed.
Pierre Gautrat, et al.,
bioRxiv - Plant Biology 2023
Quote:
... samples were incubated for 90 minutes at 37°C in a GUS staining solution supplied with 0.5 mg/mL X-Gluc (Biosynth). The staining reaction was stopped with 3:1 acetic acid/ethanol for 60minutes ...
-
No products found
because this supplier's products are not listed.
Gefei Chen, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The grids were then incubated with polyclonal antibody against human proSP-C (SFTPC) (1:200 dilution, Atlas Antibodies) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Victoria A. Bonnell, et al.,
bioRxiv - Genomics 2023
Quote:
... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
-
No products found
because this supplier's products are not listed.
Thibault Legal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cheeseman, 1: 1000), guinea pig CENP-C (pAb, MBL PD030, 1:2000) and human ACA antibodies (Cambridge Biosciences, 1:100). Hoechst 33342 (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Ilse M. Boekhoud, et al.,
bioRxiv - Microbiology 2020
Quote:
... and bromophenol blue at 95°C for 5 minutes and loaded on 8% monomeric UreaGel (SequaGel, National Diagnostics). Gels were dried and exposed to PhosphorImager screens overnight (approximately 17 hours ...
-
No products found
because this supplier's products are not listed.
Asmaa El-Kenawi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Samples were homogenized at speed 8 for 5 minutes at 4°C (Bullet Blender, Next Advance, Troy, NY) and transferred to a 5 mL glass centrifuge tube for Liquid-Liquid Extraction (LLE)(17 ...
-
No products found
because this supplier's products are not listed.
Atul Rangadurai, et al.,
bioRxiv - Biophysics 2021
Quote:
... 13C/15N labeled C (Cambridge Isotope Laboratories), 13C C2 and C8 labeled rm6A (67) ...
-
No products found
because this supplier's products are not listed.
Sonu Kumar, et al.,
bioRxiv - Microbiology 2020
Quote:
The SEC-purified Fab M4H2K1 and complex were each concentrated to 12 mg/ml before being screened at both 4 °C and 20 °C using our high-throughput CrystalMationTM robotic system (Rigaku) at TSRI (103) ...
-
No products found
because this supplier's products are not listed.
Emily K. Sims, et al.,
bioRxiv - Pathology 2019
Quote:
... Three of the donors with type 1 diabetes had detectable random serum C-peptide and 13 were classified by nPOD as C-peptide negative (random serum C-peptide <0.017nmol/L via TOSOH immunossay) (12) ...
-
No products found
Ashaq Hussain, Malay Kumar Ray,
bioRxiv - Microbiology 2022
Quote:
... syringae Lz4W was routinely grown at 22°C or 4°C (for optimum and low temperatures respectively) in Antarctic bacterial medium (ABM) composed of 5 g/l peptone and 2.0 g/l yeast extract ...
-
No products found
because this supplier's products are not listed.
H. Dihazi, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Bone Morphogenetic Protein-5 (BMP-5 – 100 ng/mL); melatonin (5 µMol/L) ...
-
No products found
because this supplier's products are not listed.
Jade Chen, et al.,
bioRxiv - Microbiology 2021
Quote:
... CDC AR Bank isolates were stored as glycerol stocks at −80°C and were grown from these stocks at 35°C on tryptic soy agar plates with 5% sheep’s blood (Hardy Diagnostics) for 18-24 hours before testing ...
-
No products found
because this supplier's products are not listed.
Erin A. Nekritz, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... we co-transfected the cells with 50 ng of pMIR-REPORT constructs containing the 3’UTR sequences together with 50 ng of a Renilla housekeeping control plasmid and 100 nM of miR-424 or miR-503 mimics or a miRNA negative control (Dharmacon #C-300717-05, #C-300841-05, or #CN-001000-01-05, respectively) using the TransIT-X2 reagent (Mirus 6003). 24 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Georg OM Bobkov, et al.,
bioRxiv - Cell Biology 2019
Quote:
... bound proteins eluted with Laemmli Buffer at 95°C for 5 min and analysed on 15% SDS-PAGE gel stained with InstantBlue™ (Expedeon).
-
No products found
because this supplier's products are not listed.
Bruna Victorasso Jardim-Perassi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001 ...
-
No products found
because this supplier's products are not listed.
Morgan L. Zenner, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
-
No products found
because this supplier's products are not listed.
Ruth M. Saecker, et al.,
bioRxiv - Biophysics 2024
Quote:
... equipped with a BioQuantum Imaging filter (slit width 20 eV) and either a K3 (tr-Spotiton datasets) or a K2 (5°C dataset) direct electron detector (Gatan, Inc., Pleasanton, CA). All tr-Spotiton grid images were recorded in counting mode with a physical pixel size of 0.844 Å ...
-
No products found
because this supplier's products are not listed.
Amine Driouchi, et al.,
bioRxiv - Biophysics 2023
Quote:
... ribonucleoprotein (RNP) complexes included sgRNAs targeting either N- or C-terminal region (Integrated DNA Technologies - IDT) and Cas9 protein (PNA bio, Cat #CP01) were transfected together with linear dsDNA donors (IDT ...
-
No products found
because this supplier's products are not listed.
Prasath Paramasivam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine)and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies). Formaldehyde (Merck) ...
-
No products found
because this supplier's products are not listed.
Tingting Yang, et al.,
bioRxiv - Biophysics 2023
Quote:
... measurements were also performed to measure conduction along Ballona Marsh CB between the IDA microelectrodes and C-AFM tip (Si Probe ASYELEC.01-R2, Oxford Instruments, with a conductive Ti/Ir (5/20) coating and 28±10 nm tip radius ...
-
No products found
because this supplier's products are not listed.
Katharina Ziegler, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Slices were maintained at 24±1 °C using a dual TC344B temperature control system (Sutter Instruments). S1HL slices were continuously perfused with oxygenated (95%O2/ 5%CO2 ...