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Citations for GenScript :
401 - 450 of 6194 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The reaction was stopped at different time points by adding Laemmli sample buffer and incubating the samples 5 min at 95 °C before loading them on Bis-Tris-SDS 4-20% polyacrylamide gels (SurePAGE, GenScript).
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bioRxiv - Biochemistry 2024Quote: ... gene was codon-optimized and cloned into the p423_GAL1 yeast expression vector as an N-terminal Flag (DYKDDDDK) and C-terminal deca-histidine (10X His) tagged fusion protein (GenScript) (Supplementary Fig ...
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bioRxiv - Biochemistry 2024Quote: ... Purified EGF-like domain of NRG1ý or BTC was incubated with anti-DYKDDDDK G1 affinity resin (Genscript, short anti-Flag) for 1 hour at 4 °C and serially washed 3x with Buffer A (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... and purification of “Ceres” were performed by GenScript (Piscataway, NJ, USA). In brief ...
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bioRxiv - Biochemistry 2024Quote: ... Tris-MOPS-SDS gels (GenScript). The resolved proteins were then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... synthesized and cloned into the pET-26b(+) expression vector using NdeI/XhoI restriction sites by GenScript (Piscataway, NJ, USA). The resulting vector was used to transform One Shot™ BL21 Star™ (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... and synthesized and cloned into the pET-DUET-1 vector with a C-terminal Tobacco Etch Virus (TEV) protease cleavage site (ENLYFQG) and hexahistidine tag (His6) (GenScript). The expression construct was transfected into E ...
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bioRxiv - Biochemistry 2024Quote: All plasmids used either were procured from GenScript or cDNA clones were obtained from Dharmacon – Mammalian Gene Collection (listed in the reagent sheet) ...
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bioRxiv - Biochemistry 2024Quote: N-terminally biotinylated synthetic FLAG peptide variants were ordered from Genscript. These were dissolved in SPR buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH was detected using an antibody purchased from Cell Signaling (Cat#97166S) and ORF1 and ORF2 were detected using antibodies generated by GenScript. Finally ...
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bioRxiv - Biochemistry 2024Quote: ... the full-length ORF sequence of eloD was ordered as a synthetic gene (Genscript Biotech, Leiden, The Netherlands), and subcloned into pYES2 as described above ...
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bioRxiv - Biochemistry 2024Quote: ... had the following sequence: KIHPSYCGPAILRFRQLLAEQEPE and was purchased by Genscript (purity >95%, checked by HPLC). The lyophilized peptide was resuspended in pure bi-distilled water at a concentration of ∼700 µM according to manufacturer instructions and stored at -80°C until use.
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were electrophoresed by pre-made SDS page (GenScript) and transferred to PVDF membranes (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... and TTK genes were introduced in PANC-1 using pcDNA3.1+/C-(K)-DYK (GenEZ; GenScript) vectors (10 µg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... R297A/L301A/R303A and R297E/R303E) were purchased from GenScript.
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bioRxiv - Cancer Biology 2024Quote: The PEAK1 peptide used for ITC was synthesized to >95% purity with acetylation at the N-terminus and amidation at the C-terminus (Genscript). The peptide sequence was as follows ...
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bioRxiv - Cell Biology 2024Quote: ... All peptides were synthesized (GenScript Biotech) at more than 98% purity ...
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bioRxiv - Cancer Biology 2024Quote: ... Bis-Tris Protein Gel (GenScript), and blotted ...
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bioRxiv - Cancer Biology 2024Quote: ... and SKP2 were synthesized by GenScript (GenScript, China) and subcloned into the lentiviral vector pLKO.1-puro (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and SKP2 were synthesized by GenScript (GenScript, China) and subcloned into the lentiviral vector pLKO.1-puro (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... The primers used in this study were synthesized by GenScript (GenScript, China). The primers are listed in Supplementary Table 2.
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bioRxiv - Biophysics 2024Quote: ... or C154Y mutants optimized for expression in yeast were cloned in a pPIC9K vector upstream of a sequence coding for a PreScission protease cleavage site (LEVLFQGP) followed by a linker of 11 amino acids and a 10His tag (GenScript). The plasmids were introduced in Pichia pastoris strain SMD1163 (his4 ...
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bioRxiv - Biophysics 2024Quote: The hKir2.1 cDNA gene coding the sequence of human Kir2.1 was cloned into the mammalian expression vector pMT3 containing an Ampicillin resistance gene (GenScript). Kir2.1 pathological mutants (C154Y ...
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bioRxiv - Biophysics 2024Quote: Macroscopic potassium currents (whole-cell currents) were recorded 2-3 days after injection of hKir2.1 cDNA (Genscript) using a two-electrode voltage-clamp amplifier (OC-725C ...
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bioRxiv - Biophysics 2024Quote: The mPRD2 construct with a C-terminal His-tag was bought from Genscript Inc ...
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bioRxiv - Biophysics 2024Quote: ... The insoluble fraction was removed by ultracentrifugation at 180,000g for 30 min and the supernatant was then incubated with Anti-DYKDDDDK M1 resin (Genscript) for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... the gene was cloned into pQE-80L vector (GenScript) and transformed onto E ...
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bioRxiv - Biophysics 2024Quote: All oligopeptide sequences were purchased from GenScript (Piscataway, NJ). Mass spectrometry data for these sequences are provided in the Supplementary Information (Supplementary Figures 1-10).
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bioRxiv - Cancer Biology 2024Quote: ... the same amount of whole cell proteins extracted from a positive sample and a control sample were individually boiled with the gel-loading buffer (4×LDS, Genscript, Nanjing, China), and loaded onto a 4 – 12% gradient SDS-PAGE gel (Genscript ...
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bioRxiv - Cancer Biology 2024Quote: ... and loaded onto a 4 – 12% gradient SDS-PAGE gel (Genscript) for electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... which were kindly provided by Professor Yu Zhang at National Institute of Biological Sciences as gifts (the sgPTPRN2 plasmid was further cloned by GenScript), were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 mM EDTA pH 8.0) and elution with 0.5 mg/ml 3xM3DK (Genscript, Piscataway NJ) peptide in 1/3 the total volume of Dynabeads for negative stain experiments and ½ the bead volume for tomography ...
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bioRxiv - Biophysics 2024Quote: ... pGEX-MgtA dimer (12mer) plasmids were de novo gene synthesized by GenScript. Plasmids created in this study were assembled using the Gibson Assembly Cloning Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... BFP-HL-60 and GFP-HL-60 were generated with lentivirus transduction of pGK-BFP (Genscript) and LeGO-V2 (a gift from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction was incubated with gluthatione agarose beads (L00206: GenScript) for 2H ...
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bioRxiv - Cell Biology 2024Quote: ... or TNFα (GenScript, Z02774-20). Human GF was treated with five cholesterol-lowering drugs (fenofibrate ...
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bioRxiv - Cell Biology 2024Quote: ... IL1β (GenScript, Z02922-10, New Jersey, USA) or TNFα (GenScript ...
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bioRxiv - Cell Biology 2024Quote: ... aureus auto inducer peptide (AIP) of 98% purity was purchased from GenScript (Piscataway, NJ, USA). Keratinocyte growth medium-2 (KGM-2 ...
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bioRxiv - Cell Biology 2024Quote: Short hairpin RNAs (shRNA) were cloned (Genscript) into HpaI and XhoI downstream of the U6 promoter of the self-inactivating lentiviral vector pLL3.7 that was modified replacing the EGFP sequence by puromycin sequence (pLL3.7-Puro) ...
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bioRxiv - Cell Biology 2024Quote: pcDNA3.1 vectors expressing human caspase-4 and human IL-18 were purchased from Genscript. Mutagenesis primers were designed using Aligent Quik change primer design ...
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bioRxiv - Cell Biology 2024Quote: The Complementary Determining Sequence (CDS) encoding PfMFS_DT was codon-optimized for expression in yeast (GenScript Biotech, United States) (Supplementary Figure S2 ...
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bioRxiv - Cell Biology 2024Quote: ... N-terminal acetylated ChREBP peptide used for crystallization was purchased from GenScript Biotech Corp ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides used for selectivity studies were purchased from GenScript Biotech Corp with the following sequences ...
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bioRxiv - Cell Biology 2024Quote: ... or 500nM III-11C (GenScript, #SC6004PF) for 24h in serum media in addition to 5ng/mL TGF-β1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium was collected 7 days post-transfection and mAbs were purified using protein A resin (Genscript) affinity chromatography and eluted in PBS (pH 7.4) ...
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bioRxiv - Neuroscience 2024Quote: ... 2000) was codon optimized for human cells and synthesized into a pcDNA 3.1(+) vector by Genscript. Codon-optimized G5A and Gqi5/9 sequences as well as cloning primers and further details are provided in Supplementary file 7.
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bioRxiv - Biophysics 2024Quote: ... TTR type was determined by transferring the gel contents to a 0.2 µm nitrocellulose membrane and probing with a primary antibody (1:1000) directed against the C-terminal region of the wild type TTR sequence (GenScript). Horseradish peroxidase-conjugated goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... sequence validated via Sanger Sequencing (Genscript), and utilized for lentivirus production through the University of Michigan Vector Core ...
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bioRxiv - Biophysics 2024Quote: ... The genes encoding for RepA(1-70)TitinX were constructed and cloned into the pET28a vector by Genscript (Piscataway, NJ). The total substrate lengths are 168 ...
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bioRxiv - Genetics 2024Quote: Peptides synthesized from GenScript. RGD purchased from R&D systems ...