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Citations for Takara Bio :
501 - 550 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Homology cloning was performed using In-Fusion® Snap Assembly Master Mix for homology cloning according to manufacturer protocols (TAKARA Bio Inc., San Jose, CA). All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... and WndC130S-KD::GFP were generated by site-directed mutagenesis using In-Fusion® Snap Assembly Master Mix (Takara Bio USA, Inc, San Jose, CA). UAS-Wnd-C130S::GFP and UAS-WndΔ101-200::GFP were generated by site-directed mutagenesis ...
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bioRxiv - Molecular Biology 2021Quote: ... The first stranded cDNA was next amplified to produce double stranded cDNA in 20 amplification cycles by long distance PCR using the Advantage 2 polymerase mix (Takara, Clontech) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was conducted with tprK-specific primers (S1 Table) appended to 16bp PacBio barcodes using CloneAmp HiFi polymerase mix (Takara) and the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
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bioRxiv - Genomics 2021Quote: ... These nanowells were selected for subsequent targeted deposition of 50□nl/nanowell RT-PCR reaction mix from the SMARTer ICELL8 3’ DE Reagent Kit (Takara) using the MSND ...
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bioRxiv - Microbiology 2021Quote: ... P3 and NP from D/660 and D/CN286 were amplified from the respective bidirectional pHW2000 plasmid constructs using CloneAmp Hifi PCR mix (Takara) with the following cycle profile ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted 1:1 with nuclease free water and subjected to qRT-PCR using SyBr green mix (Takara) in real-time PCR machine (BioRad CFX96) ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using TOYOBO THUNDERBIRD SYBR qPCR Mix on a Thermal Cycler Dice Real Time System (Takara Bio). The average threshold cycle value (CT ...
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bioRxiv - Neuroscience 2022Quote: ... the BamHI/BsrGI digested PCR product was ligated to the linearized backbone using the DNA Ligation Kit - Mighty Mix (TaKaRa) resulting pPB-ef1a-NLS-EGFP-T2A-puro.
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bioRxiv - Molecular Biology 2021Quote: ... The first stranded cDNA was next amplified to produce double stranded cDNA in 20 amplification cycles by long distance PCR using the Advantage 2 polymerase mix (Takara, Clontech) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: For the V1 protocol 20 µL of pooled and ExoI treated cDNA was PCR amplified with Advantage 2 Polymerase Mix (Clontech, #639206) in 50 µL total reaction volume using 1 µL of 10 µM LA_oligo (Microsynth ...
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bioRxiv - Microbiology 2021Quote: ... The Plus25neutral mutant was gener- ated via Gibson assembly using CloneAmpTM 2X HiFi PCR mix (Takara Bio USA, Mountain View, CA) for backbone amplification ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification on the cleaned libraries was performed as follows: A reaction mix using LA Takara Hot Start kit (Takara Bio) was prepared according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR was performed using THUNDERBIRD Next SYRB qPCR Mix (TOYOBO) on a real-time PCR system (Thermal Cycler Dice® Real Time System, Takara).
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bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA used for qRT-PCR were synthesized using PrimeScript reverse transcriptase with oligo dT primer and Prime Script RT Enzyme MIX I (Takara, Osaka, Japan). qRT-PCRs was performed using the ChamQ SYBR Color qPCR Master Mix (Q411 ...
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bioRxiv - Zoology 2021Quote: ... and cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio Inc.). The amount of target RNA was normalized to an endogenous control ribosomal protein 49 gene (rp49) ...
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bioRxiv - Molecular Biology 2022Quote: ... Random Primer Mix (Takara) and 200 U SMARTScribe Reverse Transcriptase (Takara) ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR Green Mix (TaKaRa) was used for quantitative PCR ...
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bioRxiv - Microbiology 2023Quote: ... Mighty Mix (Takara Bio) was used for ligation ...
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bioRxiv - Immunology 2022Quote: ... Real-time RT-PCR analyses for viral RNA copy number was carried out with One Step PrimeScript™ III RT-qPCR Mix (Takara, Cat# RR600B) and reactions were performed by using LightCycler® 96 System (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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bioRxiv - Microbiology 2022Quote: RT-PCR was performed in a single closed tube using a one-step RT-PCR kit (One Step PrimeScript III RT-qPCR Mix, with UNG; Takara Bio Inc., Kusatsu, Japan) in accordance with the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: SARS-CoV-2 cDNA was generated from 100 ng of RNA in a RT-PCR reaction buffer containing 4 μL of 5X PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio, Kusatsu, Shiga, Japan), 2 μL of 50 μM random hexamer primer ...
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bioRxiv - Cancer Biology 2020Quote: ... 3mM dNTP mix (Takara Bio)) ...
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bioRxiv - Cell Biology 2021Quote: ... Mighty Mix (Takara Bio, 6023) and transformed into One Shot TOP10 Chemically Competent E.coli (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-GGGRCCARKG GATAGACHGATGG-3’ (designed according to the C segment of mouse IgG antibody heavy chain sequence ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-5’ s-CTTCAGAGGA AGGGTGGAAACAGG-3’ (designed according to the C segment of mouse IgG antibody light chain sequence ...
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bioRxiv - Microbiology 2021Quote: ... using FastStart Universal SYBR Green Master (Takara). The level of β-actin expression was used to normalize the data ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs from Mouse Total RNA Master Panel (Takara) were reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... and 3× PrimeScript enzyme mix (TAKARA) were added to the purified nucleic acids for reverse transcription ...
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bioRxiv - Immunology 2022Quote: ... with a SYBR Green mix (Takara) and predesigned primers obtained from Sigma-Aldrich (KiCqStart® Primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... SYBR Pre-mix Ex Taq (Takara) was used for RT-qPCR following the manufacturer’s instructions and the reaction was performed in the CFX96 TouchTM Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... 5× PrimeScript™ RT mix (TaKaRa) was used to acquire cDNA ...
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bioRxiv - Genomics 2023Quote: ... 12.5µL 2X CloneAmp Pre-Mix (Takara) and 1µL each 10µM of tpdel-F (5’-TGCTCTCGCATGGCTATGTT-3’ ...
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bioRxiv - Genetics 2022Quote: ... then combined with 6 µL cDNA and 15 µL Mighty Mix T4 DNA ligase reaction mix (Takara) and incubated overnight at 16 °C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR (CloneAmp HiFi PCR Premix, Takara-Bio Europe) genotyping was done with primers designed for each knock-out line that allowed for confirming either the presence of the wild-type (F-gt5/R-gt5WT and F-gt3WT/R-gt3 ...
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bioRxiv - Plant Biology 2023Quote: ... we PCR amplified (CloneAMP HIFI PCR premix, TAKARA) the coding sequences of ATG8a ...
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bioRxiv - Immunology 2021Quote: ... The mix SYBR Green Premix ExTaq (Takara) was used for quantitative PCR in an iCyclerMyiQ thermocycler (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR mix (Takara Bio Inc., Japan), with primers and probe specific for the SARS-CoV-2 E gene ...
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bioRxiv - Plant Biology 2020Quote: ... using DNA Ligation Kit
Mix> (Takara). The constructed vector was transformed into the DH5α strain of E.coli ... -
bioRxiv - Microbiology 2020Quote: ... or Advantage 2 Polymerase mix (Takara Bio) for amplification of UTRs and Pfmyob ...
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bioRxiv - Genetics 2022Quote: ... Mighty Mix T4 DNA ligase from Takara; Dual luciferase reporter assay from Promega ...
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bioRxiv - Cell Biology 2021Quote: ... using Lentiviral High Titer Packaging Mix (Takara) as described previously75 ...
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bioRxiv - Cell Biology 2023Quote: ... and ligated using 2x Ligation Mix (Takara) and checked for insert orientation ...
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bioRxiv - Genomics 2020Quote: ... as well as the Mouse total RNA Master panel (Clontech), were used to study the level of expression of candidate lncRNAs ...