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Citations for Takara Bio :
701 - 750 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR kit (Takara, Tokyo, Japan) was used to construct reverse transcription to collect cDNA ...
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bioRxiv - Molecular Biology 2023Quote: CloneAmp™ HiFi PCR Premix (Takara) and 1 µl DMSO were mixed in a total volume of 25 µl H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: ... including PCR using PrimeSTAR GXL (Takara), and DpnI treatment (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using PrimeStarMax (TaKaRa), and the resulting products were checked on agarose gel (1% ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was performed on a TP-850 Real-Time PCR Detection System (TAKARA Bio) using SYBR Green Premix ExTaq II ...
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bioRxiv - Immunology 2022Quote: ... and quantitative real-time PCR (q-PCR) was carried out using SYBR Premix Ex Taq (TaKaRa, Japan). Q-PCR was carried out in a Bio-Rad CFX96 system ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse PCRs were performed in two rounds using CloneAmp HiFi PCR premix (Clontech, Mountain View, CA, USA). The first round was performed adding the forward and reverse primers in two independent reactions and consisted of 3 cycles of 98 °C for 10 seconds ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using a One Step TB Green PrimeScript RT–PCR Kit II (Takara, #RR086A) on a LightCycler 2.0 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... and MSN4 were measured by RT-PCR using One Step SYBR PrimeScript RT–PCR Kit II (TaKaRa). Reactions were run on Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using the Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio, USA) using the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The real-time PCR reaction was performed using One Step SYBR Prime Script RT-PCR Kit (TakaRa). The primers for 14-3-3β ...
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ul of the cDNA was used for PCR reaction using CloneAmp HiFi PCR Premix (Takara, 639298) with the following primers ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of dNTP mix (TaKaRa, 2.5 mM concentration, 200 µM final), 0.125 µl of HotStart ExTaq (TaKaRa ...
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bioRxiv - Molecular Biology 2019Quote: ... the digested genomic DNA was used for qPCR (TAKARA mix for qPCR) with the primers listed in the table below.
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bioRxiv - Microbiology 2019Quote: ... ISC5 and ISC5 groups using Advantage 2 Polymerase Mix (TAKARA, Kusatsu, Japan). 2µl of template with varying DNA concentration were used in a total of 50µl PCR reaction (S1 Dataset A) ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 nL lysis mix was added (0.8 U RNase inhibitor (Clontech, 2313A), 0.07% Igepal ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using with the SYBR Premix Ex Taq mix (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara) and Thermal Cycler Dice Real Time System II (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed using the TB Green Fast qPCR Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara) ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara). For each sample ...
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bioRxiv - Molecular Biology 2019Quote: ... and both long (NM_001300829) and short (NM_001280) isoforms of CIRBP were cloned by PCR (HiFi PCR premix, Clontech) from cDNA from Huh7 cells prepared with the Superscript III RT kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... The quantification of gene transcripts was performed by quantitative PCR (Q-PCR) using SYBR Premix Ex Taq (TAKARA). All values were normalized to the level of β-actin mRNA ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Immunology 2020Quote: ... q-RT-PCR was performed using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time; TaKaRa). The primers used for q-RT-PCR were selected to be specific for the E and ORF1ab sequences in the SARS-CoV-2 genome (Table 1) ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products covering the whole sesRNA region was generated with CloneAmp HiFi PCR Premix (Takara, Cat. No. 639298), and purified with NucleoSpin Gel and PCR Clean-up kit (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc.) and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.) ...
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Genomics 2020Quote: ... and 10 μL PCR Mixture (TaKaRa, Japan). PCR reaction was conducted on a T100 thermocycler (Bio-Rad ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... using PCR and In-Fusion cloning (Clontech). Human codon optimized Cas9 nuclease (hCas9 ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Microbiology 2019Quote: ... PCR analyses were performed using ExTaq (Takara) according the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The Advantage GC 2 PCR Kit (Takara) was used for gene cloning ...
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bioRxiv - Neuroscience 2020Quote: Using a PCR method and Pyrobest (Takara), the serine residues encoding S816 ...
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bioRxiv - Biochemistry 2020Quote: CloneAmp HiFi PCR Premix was from Takara. Restriction enzymes were from New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... We then performed LaTaq PCR (Takara, RR002A) to amplify the β-catenin transcripts ...
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bioRxiv - Genomics 2019Quote: ... and an Advantage 2 PCR Kit (Clontech) were used for cDNA generation ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was conducted using PrimeSTAR polymerase (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or CloneAmp HiFi PCR Premix (Clontech, USA). Amplicons were separated on a 1-2% agarose gel and appropriate bands were excised and isolated using a gel extraction kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and CloneAmp HiFi PCR (Takara, Tokyo, Japan) according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2022Quote: ... The SMARTer PCR cDNA Synthesis Kit (Takara) was used for first-strand cDNA synthesis ...
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bioRxiv - Neuroscience 2022Quote: ... on a PCR thermal cycler (TP350, TaKaRa)57,58 ...