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Citations for Takara Bio :
401 - 450 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and first-strand cDNA was synthesized using PrimeScript RT Master Mix (Perfect Real Time) (RR036Q; TaKaRa, Ohtsu, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNAs was synthesized from total RNA using the PrimeScript™ RT Master Mix (Perfect Real Time, Takara). Quantitative real-time PCR was performed in triplicate using PowerUp SYBR Green (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were subcloned into pGEMHE (22) using the In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) with gene-specific primers and 15-bp sequences complementary to the ends of the linearized pGEMHE (Table S4 ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was further amplified by adding 40 μl of a PCR mix containing 0.03 U/μl of Terra PCR direct polymerase (Takara Bio), 1.25x Terra PCR Direct Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNA target locus on exon 3 or 5 was PCR amplified with Terra™ PCR Direct Polymerase Mix (Takara) for 35 cycles using primer sets mEX3F and mEX3R or mEX5F and mEX5R (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification with Gateway cloning compatible primers was performed directly on Aag2 gDNA using CloneAmp Hifi PCR pre-mix (Takara), without prior amplification in a TOPO TA cloning vector ...
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bioRxiv - Molecular Biology 2022Quote: Two hundred nanogram of DNAs were used as a template for polymerase chain reaction (PCR) by using EmeraldAmp MAX PCR mater mix (Takara). First 30 cycles of PCR were performed using forward and reverse primers below ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was amplified by long distance PCR amplification using Advantage polymerase mix (TaKaRa). The amplified ds cDNA was purified using CHROMA SPIN TE-400 column which retains and traps the DNA molecules of less than 200 bp size ...
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bioRxiv - Neuroscience 2023Quote: ... Synthesized cDNA was subjected to quantitative PCR using TB Green Fast qPCR Mix (Takara) on the LightCycler system (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM, Clontech) with KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of isolated gDNA was amplified using the PrimeSTAR MAX PCR mix (Takara) using the primers gRNA_screening_FW and gRNA_screening_RV (Table1) ...
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bioRxiv - Bioengineering 2021Quote: ... To verify deletions or test for the presence of the CPOT plasmids colony PCR was performed using SapphireAmp fast PCR mix (TaKaRa Bio). For DNA construction ...
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bioRxiv - Developmental Biology 2023Quote: ... At 48 hpf the GFP + progeny was genotyped using a forward primer in the foxo1a endogenous locus and a reverse primer in the EGFP inserted transgene using a touch-down PCR with the Terra™ PCR Direct Polymerase Mix (639270; Takara). The PCR product was run in a 1% agarose gel to validate its size and the remaining product was sent for Sanger sequence.
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bioRxiv - Immunology 2021Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara Bio Europe, France). Quantitative real-time PCR was performed at 60°C using Takyon ROX SYBR MasterMix (Eurogentec ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed into cDNA using Takara PrimeScript RT master mix (RR036A; Takara Biotechnology Co., Ltd., Dalian China). RT-qPCR was performed using an ABI StepOne Plus Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA (1 μg) was applied to generate cDNA by means of PrimeScript™RT Master Mix (Takara, RR036A). The real-time quantitative PCR was performed by using SYBR Green Master Mix (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... The conventional PCR was performed separately for the genes in a 25µl volume with 12.5µl 2x Emerald GT amp master mix (TaKaRa, Cat# RR310A) along with 10 picomole of each of the forward and reverse primers for the respective genes ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA was assayed with ReverTra Ace qPCR Master Mix (TOYOBO, FSQ-201) in a Thermal Cycler Dice (TaKaRa) with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 μg of RNA using PrimeScriptTM RT Master Mix (catalog no. RR036A, Takara). qPCR was performed using TB Green Premix Ex TaqTM (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA synthesis from at least three biological replicates was performed using the PrimeScript™ RT Master Mix (TaKaRa, #RR036A) and real-time PCR was performed using SYBR Green I Master (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Then 50 nl of primer pair solution mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were loaded onto the SmartChip ...
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bioRxiv - Microbiology 2022Quote: ... 50 nl each of the DNA samples mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were first loaded onto the SmartChip MyDesign Chip ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 300 ng was used as template for the reverse transcription using the PrimeScriptTM RT Master mix (#RR036A, Takara, France). The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272 ...
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of total RNAs from each sample were reversely transcribed with PrimeScript RT Master Mix (TaKaRa, RR036A). Diluted cDNAs were mixed with primers and SsoAdvanced Universal SYBR® Green Supermix (BioRad ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... and then reverse-transcribed to cDNA using the PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of each RNA sample was reverse-transcribed with PrimeScript RT Master Mix (Perfect Real Time) (Takara Bio). qPCR was performed using the QuantiTect SYBR Green PCR Kit (QIAGEN) ...
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bioRxiv - Plant Biology 2024Quote: 0.1uL DNA templates were mixed with 10uL Emerald Amp Max PCR Master (Takara Bío. co., Japan) and 0.2uL of 10uM primer set up to 20ul with distilled water for 1st PCR analysis ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2020Quote: ... 5X Primer Mix (P5 and P7 primers) and Terra™ PCR Direct Polymerase Mix 0.05 U/ µl at reaction (Takara Bio USA, CA, USA) via a thermal protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Second strand synthesis and PCR amplification was done using the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Genetics 2023Quote: ... Second strand synthesis and PCR amplification was done by adding Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed with 2x SYBR green mix (#RR420L, Takara Bio, Otsu, Shiga, Japan) and primers against JAK1 ...
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bioRxiv - Plant Biology 2020Quote: ... PrimeScript® RT Master Mix Perfect Real-Time and DRR820ASYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from cells or tissues with TRIzol Reagent (Life) and then used for reverse transcription by PrimeScriptTM RT Master Mix (TaKaRa). Synthesized cDNA was used for qPCR by 2× ChamQTM Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was pre-amplified by adding 2 uL of cDNA from each sample to 8 uL of preamp master mix [5 uL TaKaRa premix Taq polymerase (Clontech), 2.5 uL 0.2X Taqman pooled probe ...
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bioRxiv - Molecular Biology 2021Quote: ... First-strand cDNA was synthesized using about 1µg of total RNA and RT Master Mix with gDNA Remover (Takara Bio), which contains M-MLV reverse transcriptase ...
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bioRxiv - Immunology 2021Quote: ... and equal amounts of RNA for each sample were then reversed-transcribed using the PrimeScript™ RT Master Mix (Takara), following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (same as used for generating the sequencing libraries) were reverse transcribed to cDNA using PrimeScript RT Master Mix (TaKaRa). cDNA ...
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bioRxiv - Bioengineering 2022Quote: ... were reverse transcribed into cDNA in a volume of 10 µl using the PrimeScript RT Master Mix (Perfect Real Time, TaKaRa) (37°C ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated as described in the RNA-seq section and 500 ng was reverse transcribed using the PrimeScript RT Master Mix (Takara). A volume of cDNA corresponding to 5 ng of the input RNA was subjected to quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... All the reactions were performed in duplicates in Premix Ex Taq Probe qPCR Master Mix (2X) (TaKaRa, Japan Cat#RR390) in a final volume of 25µl per tube in a CFX96 Real time PCR system® (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2022Quote: ... magnanima was assessed by PCR amplifying a cifB-like gene (Extended Data Table 3) in the WOwHm-t76 region with the Emerald Amp Max Master mix (TaKaRa) at 94°C for 3 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was reverse transcribed to complementary DNA (cDNA) using a Prime Script™ RT Master Mix Kit (TaKaRa, China) and the following procedure ...
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bioRxiv - Cell Biology 2022Quote: ... The purified fragments were integrated into digested vectors by using In-fusion Snap Assembly Master mix (Takara Bio, Japan, 638947). Our lab stock plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP) gene using the In-Fusion Snap Assembly Master Mix (TaKaRa). Forty-eight hours after transfection ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from retinas with TRIzol Reagent (Life) and then used for reverse transcription by PrimeScript RT Master Mix (TaKaRa). Synthesized cDNA was performed with 2× ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Microbiology 2023Quote: ... the CPEC cloning assembly reaction was performed with the purified MotA-ASR insert and linearized vector using In-Fusion Snap Assembly master mix (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... siRNA Knockdown efficiency was tested as follows: 200 ng of RNA was reverse transcribed with Prime Script RT Master Mix (Takara) and quantified using QuantiFast SYBR Green RT-PCR Kit (Qiagen ...