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Citations for Takara Bio :
451 - 500 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... cDNA was generated from each sample employing the Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc., Japan). Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc. ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947) and following recommended manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
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bioRxiv - Biochemistry 2019Quote: ... RT was performed using Oligo(dT) random 6-mer primers from a Prime Script RT Master Mix kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted using a Plant RNA Kit (R6827, Omega) and then reverse transcribed using PrimeScript™ RT Master Mix (RR036Q, Takara) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... was used to generate templates to synthesize first chain cDNA using the PrimeScript™ RT Master Mix kit (TAKARA, Dalian, China), with three biological replicates sample ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of total RNA was used for the cDNA synthesis reaction using PrimeScript™ RT Master Mix kit (TaKaRa RR036A) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was reverse transcribed into 10 μL of complementary DNA (cDNA) with PrimeScriptTM RT Master Mix (Takara Biomedical Technology, China) at 37°C for 15 min ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of the cDNA was used as DNA template in 15-µL amplification volumes with 400 nM of each primer and 7.5 µL of SYBR green master mix (Takara, Beijing, China) using the following cycling parameters ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned it (in frame) into the linearized LV-GFP plasmid using In-Fusion® Snap Assembly Master Mix (Takara #638947). High titer viruses were generated as previously described 56 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The transcripts were quantified by using SYBR Green Master Mix: SYBR green based TB Green Premium Ex Taq II (Takara, RR82WR) in the PCR instrument Light Cycler 480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized from purified total RNA using PrimeScript RT Master Mix (Perfect Real Time; Takara Bio, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sample preparation for RT-qPCR from primary cultured neurons was performed using the RNeasy Mini Kit and PrimeScript RT Master Mix (Takara Bio). RT-qPCR was performed using KOD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from these retained samples was reverse-transcribed into cDNA using 250 ng as a template and the PrimeScriptTM RT Master mix (#RR036A, Takara, France). To provide a comparison point for the allele-specific expression in F1 larvae ...
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bioRxiv - Microbiology 2024Quote: ... and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Cancer Biology 2021Quote: Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
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bioRxiv - Physiology 2021Quote: ... Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was performed using the Probe qPCR Mix (Takara Bio, Otsu, Shiga, Japan) and Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Cell Biology 2023Quote: ... Second strand synthesis and PCR amplification was performed by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Neuroscience 2021Quote: ... the cDNA samples were synthesized from 500 to 1000 ng total RNA using the PrimeScriptTM RT Master Mix cDNA (Takara, Kyoto, JAP) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was perfor med using a qPCRsoft (Analytik Jena AG, Germany) with a SYBR®Green PC R Master Mix Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from organoids using Nucleo Spin (MACHEREY NAGEL) and cDNA was synthesized from 500 ng of total RNA using PrimeScript™ RT Master Mix (TAKARA BIO). We performed quantitative reverse-transcription PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of total RNA was used to produce cDNA using the TaKaRa PrimeScript RT master mix (TaKaRa Bio, Otsu, Japan). Two microliters of cDNA was then used for quantitative PCR ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then frozen and sent to Novogene for ultra-low-input bulk RNA sequencing or converted into cDNA with the PrimeScript RT Master Mix (Takara Bio, RR036A) for quantification of mRNA level using qPCR4 ...
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bioRxiv - Microbiology 2023Quote: Total RNA from intestinal tissue and organoids was extracted using TRIzol method according to the precise instructions and cDNA was synthesized using PrimeScript™ RT Master Mix (Takara, Japan). With cDNA as template ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA libraries for each sample were constructed from 100 ng of RNA using the PrimeScript RT master mix (Takara Bio, Kutatsu, Japan) under manufacturer specifications ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... sublines 14D7 and 24C7.8 The presence of the deletion on the translocated and the wildtype allele was validated by PCR using the Terra PCR Direct Polymerase Mix (Takara Bio Europe; supplemental Table 7). From line 14D7 ...
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bioRxiv - Molecular Biology 2022Quote: ... the JAK3 open reading frame was amplified by PCR using the Advantage 2 polymerase mix (Takara Bio) with JAK3-ORF primers (Table 1 ...
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bioRxiv - Pathology 2022Quote: ... Quantitative real-time PCR was performed by TB Green Fast qPCR Mix (Takara Bio Inc. Kusatsu, Japan) on a PCR System (Roche ...
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bioRxiv - Genomics 2024Quote: ... The PCR reaction mix consisted of 1.25 PrimeSTAR GXL DNA Polymerase (Takara Bio USA, San Jose, CA), 1× Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... YFP-FKBP and Tom20-CFP-FRB were inserted to the digested sites using In-Fusion Snap Assembly Master Mix (Takara Bio USA, 638947). To visualize Golgi ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... The insert was cloned into the pAM_1C vector using the In-Fusion snap assembly master mix according to manufacturer’s instructions (Takara Bio, Kyoto, Shimogyō-ku, Japan). The purified plasmid was verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... with its native promotor and was subcloned into a modified pACYC vector using In-Fusion cloning strategy (In-Fusion® Snap Assembly Master Mix; TaKaRa Cat. # 638947). The DNA of P ...
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bioRxiv - Immunology 2019Quote: ... The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara). Data were normalized to the expression of the housekeeping gene β-actin ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR analysis was performed using the ABI7500 machine and SYBR Premix Ex Taq Mix (Takara #RR420A). LOC_Os06g48970 was used as an internal reference as described previously (Dreni et al. ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...