Labshake search
Citations for Takara Bio :
301 - 350 of 780 citations for Family With Sequence Similarity 46 Member B FAM46B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... pLPCX-IRES-eGFP was generated by cloning a fusion PCRP consisting of the encephalomyocarditis virus internal ribosomal entry site (EMCV-IRES) upstream of the eGFP coding sequence flanked by EcoRI and NotI sites into the cognate sites of pLPCX (Clontech). A DNA gene block encoding the vaccinia virus Wisconsin strain K3L fused to the C-terminus of mNeonGreen by a GSGS linker and hosted into the expression vector pTwist Lenti SFFV puro WPRE was obtained commercially (Twist Bioscience) ...
-
African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).
-
bioRxiv - Molecular Biology 2021Quote: ... SmRho1.1-pCR4-TOPO and SmRho1.2-pCR4-TOPO were cut by BamHI and NdeI and the sequences were inserted in frame into the pGADT7-AD expression vector (Clontech) using the T4 DNA Ligase (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
-
bioRxiv - Biophysics 2020Quote: ... The peroxisome-targeting PEX3-mRFP-FKBP construct was a gift from Casper Hoogenraad (Utrecht University (53)).Constructs coding for FRB (DmrA) and FKBP (DmrC) sequences were obtained from ARIAD Pharmaceuticals and are now available from Takara Bio Inc ...
-
bioRxiv - Biophysics 2021Quote: ... GRB2-HaloTag was constructed using the human GRB2-encoding fragment and inserted into the Halo7-C2 vector in which the monomeric EGFP sequence in the pEGFP-C2 vector (Clontech) was substituted to the Halo7 sequence from the FN19K HaloTag T7 SP6 Flexi Vector (Promega).
-
bioRxiv - Immunology 2022Quote: ... The pINDUCER20-EYFP-mCITED1 lentiviral construct was produced by amplifying the full-length murine CITED1 coding sequence from pCDNA3-Flag-mCITED1 and subcloning it into pEYFP-C1 (Takara/Clontech) in-frame with the EYFP coding sequence ...
-
bioRxiv - Cell Biology 2022Quote: ... Constructs coding for FRB (DmrA) and FKBP (DmrC) sequences were obtained from ARIAD Pharmaceuticals and are now available from Takara Bio Inc ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CDC14Br (Table S4) and inserted in place of SV40 UTR and poly (A) sequences in pGL3-SV40 using in-Fusion (Takara). Mutations were generated in 3’ UTR sequences using In-Fusion and mutagenic primers PCGF3-inf-f ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP-Vti1a and GFP-DNAJC5 were generated by amplifying the corresponding sequences from a HUVEC cDNA library and cloned each into pEGFP-C1 vector (Clontech); both were provided by Johannes Nass (University of Münster ...
-
bioRxiv - Immunology 2022Quote: ... PGDM1400 heavy and light chain sequences were synthesized (Integrated DNA Technologies) and cloned into a mammalian expression vector under a CMV promoter using InFusion (Takara) and sequence verified.
-
bioRxiv - Developmental Biology 2022Quote: Candidate enhancer sequences identified from the integrated analysis from scRNA-seq and scATAC-seq were PCR amplified (TAKARA, PrimeSTAR GXL) from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega ...
-
bioRxiv - Plant Biology 2022Quote: The coding sequence of ZmEREB57 was obtained and digested by EcoRI and BamHI and inserted into the pGADT7-AD vector (Clontech) containing the GAL4 active domain ...
-
bioRxiv - Microbiology 2022Quote: ... and 3′ sequences (3F) flanking the gene ORF were amplified from Ct3 genomic DNA by PrimeSTAR HS DNA polymerase (TaKaRa). The purified PCR products 5F and 3F were mixed with SalI-treated pBIG4MRHrev for In-Fusion reactions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Probes for FLuc (500 bp) were generated using gel-purified PCR amplicons containing GFP sequence and a BcaBEST Labeling kit (Takara) and [α-32P]-dCTP (PerkinElmer) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
-
bioRxiv - Microbiology 2023Quote: ... the pTAC promoter and coding sequence of the fluorescent markers were amplified from pLM426 derivatives (Supplemental table S3) and combined with the pBCC0029 recipient plasmid including a terminator sequence by In-Fusion HD from Takara. All plasmids for this study were purified using the NucleoSpin Plasmid kit (Macherey-Nagel) ...
-
bioRxiv - Biochemistry 2023Quote: ... the Tm1 coding sequence was inserted into MCS2 of pFastBacDual-HisSUMO- SNAP-3C-Khc between SmaI and KpnI sites by InFusion cloning (Takara). Tm1 FL for motility assays was cloned with a HisSUMO-SNAP-tag between SacI and HindIII sites of the pET11 vector by InFusion cloning (Takara) ...
-
bioRxiv - Biophysics 2023Quote: ... A plasmid expressing mouse GR tagged in the N-terminus with mCherry was developed by amplifying mouse GR coding sequence and in-frame cloning in pmCherry-C3 (Clontech). Point mutations and deletions were introduced using the Quickchange XL mutagenesis kit (Agilent ...
-
bioRxiv - Plant Biology 2023Quote: The nucleotide sequence (around 500 bp long) specific to the NlCA coding region was cloned into the pMD 19-T vector (TAKARA). The double-stranded RNA was synthesized through PCR amplification by using the Mega script T7 High Yield RNA Transcription Kit (Vazyme ...
-
bioRxiv - Cell Biology 2023Quote: ... The cDNA for p53 WT or p53 QM was cloned together with the DNA sequence for an NH2-terminal FLAG tag into pRetroX-Tight-Pur (Clontech). Retroviruses with the vesicular stomatitis virus–G (VSV-G ...
-
bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
-
bioRxiv - Microbiology 2023Quote: We first cloned the eGFP sequence between the AvrII and EagI sites of the pEOE-attP vector (53) using In-Fusion cloning (Clontech). KAHRP ...
-
bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
-
bioRxiv - Cell Biology 2023Quote: ... or deleted of the entire netrin sequence (mCherry) were amplified by PCR followed by homologous recombination according to the inFusion deletion protocol (Clontech; dLamVI primers ...
-
bioRxiv - Biochemistry 2023Quote: Full-length HSF1 and its sequences were obtained by the rapid amplification of cDNA ends using a SMART cDNA amplification kit (Clontech). Gene-specific primers (GSPs ...
-
bioRxiv - Neuroscience 2023Quote: ... PCR reaction was prepared using gene-specific primers (20µM, Eurofins, primer sequences in Suppl. Table 4) and PrimeSTAR® Max DNA Polymerase (Takara) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: Sequence of the mouse variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio, USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma based on isotype ...
-
bioRxiv - Microbiology 2023Quote: ... Amplified DNA encoding the sequence for H2A was ligated in frame with the BglII and SalI restriction digest sites of pEGFP-C1 (Clontech). To construct the pEGFP-H2A.X expression plasmid ...
-
bioRxiv - Cell Biology 2024Quote: ... The PCR amplified metr-1(cDNA) sequence was then fused with the linearized plasmid carrying the Prgef-1 promoter through InFusion cloning (Takara) methodology ...
-
bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
-
bioRxiv - Biochemistry 2024Quote: ... The codons S85 and N86 were designed as the recognition sequence (TTCGAA) of BspT104 I (Takara Bio Inc., Shiga, Japan) for further modification of the linker insertion ...
-
bioRxiv - Cancer Biology 2024Quote: ... were subcloned and fused with the CD8 signal peptide sequence (MALPVTALLLPLALLLHAA) followed by a Myc-tag at the N-terminus in pIRESpuro3 (Clontech). Humanized EREG mAbs H231 and H206 (23 ...
-
bioRxiv - Biochemistry 2020Quote: ... Amplified genes were cloned into an altered pFastBacHT B vector using the In-Fusion HD EcoDry Cloning Kit (Clontech). After transformation of DH10EMBacY E ...
-
bioRxiv - Microbiology 2020Quote: ... DNA fragments corresponding to MV-C or CH coding sequences were amplified using pTM3-MVSchw or pmCherry (Clontech, Palo Alto, USA) as a template ...
-
bioRxiv - Immunology 2021Quote: ... and F1 and F2 arms homologous to upstream and downstream sequences of the A41L ORF using in-fusion cloning (Takara Bio). The resultant spike transfer cassette was linearized by NotI and transfected into the MCL for homologous recombination with SCV ...
-
bioRxiv - Microbiology 2021Quote: ... and a linker sequence (5’-GGTAGCGGCAGCGGTAGC-3’) were added through three additional PCR reactions using PrimeStar GXL DNA polymerase (Takara Bio). PCR products were gel-purified in each step ...
-
bioRxiv - Microbiology 2019Quote: ... Proviral DNA sequences of resistant HIV-1 variants were extracted from the infected into MT4 cells using NucleoSpin® Tissue (TAKARA). DNA sequences of the IN regions were amplified with 1st primers (5’-AAA TTT AAA TTA CCC ATA CAA AAG GAA ACA TGG GAA GC-3’ and 5’-GGT CTG CTA GGT CAG GGT CTA CTT GTG TGC TAT ATC-3’ ...
-
bioRxiv - Plant Biology 2020Quote: ... The RAV1 motif enriched promoter sequence of each of the gene (Table S1) was cloned in pAbAi bait vector (Clontech, USA) in the upstream of Aureobasidine A ...
-
bioRxiv - Biochemistry 2020Quote: ... uppercase for LEU2-specific sequences and lowercase for SEC61-specific sequences) was inserted between 125 bp and 126 bp downstream of the Sec61 stop codon by In-Fusion cloning (Takara Bio). Pore mutations (M90L/T185I/M294I/M450L ...
-
bioRxiv - Biophysics 2021Quote: ... The rTetR protein sequence was subcloned from the Tet-On transactivator used in the commercially available Tet-On 3G system (Takara Bio). FUSN was derived from Addgene #122148 and GBP was based on a previously described construct (Rothbauer et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... and digested with NotI and MluI enzymes (restriction sites introduced via the PCR primer sequences) for introduction into similarly-digested pRetro-X-Tight (Takara Bio). The final insertion was confirmed by sequencing.
-
bioRxiv - Cell Biology 2021Quote: Plasmids for the pull-down assay were constructed by cloning human Kif7 and Gli2 sequences into pCMV-BICEP™-4 expression vector followed by truncations using InFusion HD Cloning Kit (Takara). Kif7 fragments were cloned at the MCS1 to express FLAG-tagged Kif7 protein and Gli2 fragments were cloned at the MCS2 to express c-myc-tagged Gli2 protein ...
-
bioRxiv - Microbiology 2021Quote: ... Genetic sequences were synthesized/amplified with homology overhangs complementary to the overhangs of EcoRI+BamHi digested pLVX-TetOne-Puro (Takara # 631849). 100ng of cDNA were ligated into 75ng of gel-purified digested vector using Neb builder (New England Biolabs #E2621 ...
-
bioRxiv - Cell Biology 2021Quote: ... The coding sequences of Arfs were amplified by PCR (primers listed in Suppl. Table S1) and inserted into the pQXCIP plasmid (Takara Bio) using the AgeI and BamHI restriction sites ...
-
bioRxiv - Neuroscience 2022Quote: ... TagBFP-P2A-Pre-Sphere-SF-iGluSnFR and mCherry-P2A-Post-Sphere-SF-iGluSnFR sequences were inserted into the pAAV-hSyn-EGFP Vector by replacing the EGFP using Infusion (Takara Bio). AAV serotype 2 was amplified in HEK293T cells by transfecting pAAV.hSynapsin.TagBFP-P2A-Pre-Sphere-SF-iGluSnFR or pAAV.hSynapsin.mCherry-P2A-Post-Sphere-SF-iGluSnFR via the pRC2-mi342 and pHelper vectors ...
-
bioRxiv - Biochemistry 2022Quote: ... The barcodes were introduced immediately after the Tae1 coding sequence via Gibson assembly cloning (In-Fusion HD Cloning Kit – Takara Bio). Our Next-Generation sequencing strategy comprised two stages ...
-
bioRxiv - Molecular Biology 2020Quote: Specific DNA sequences were PCR amplified using gene specific primers and cloned into vectors using PrimeSTAR HS DNA polymerase (#R010A, Takara-bio) and T4 DNA ligase (#M0202S ...
-
bioRxiv - Cell Biology 2020Quote: ... putative miR-31-5p binding sequences were deleted in the plasmid DNA using PrimeSTAR Mutagenesis Basal Kit (TaKaRa Bio, CA, USA) according to the manufacturer’s instructions ...