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Citations for Takara Bio :
401 - 450 of 780 citations for Family With Sequence Similarity 46 Member B FAM46B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... or GUUUGx6 250 bases downstream of the alternative exon of the RG6 splicing reporter43 (see sequences below, with altered nucleotides in capitals) using custom-designed oligonucleotides (IDT) with InFusion cloning (Takara Bio #638920). The GFP of a pEGFP rbFOX1 plasmid (Addgene #63085 ...
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bioRxiv - Pathology 2019Quote: ... pMuNoV-MNV-1 was constructed by exchanging the MNV sequence portion from MNV-1 to MNV-S7 with the In-Fusion cloning system (Takara Bio Inc.), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs with the correct sequence were then subcloned into the doxycycline-inducible lentiviral vector pLVX-Tight-puro (Clontech, Mountain View CA, USA) using BamH1 and EcoR1 restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... The forward primer contained a Kozak sequence and both primers contained 15-bp overhang with homology to the plasmid for InFusion cloning (Takara Bio, 638947). To make pmCherry-N1-Gal3 ...
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bioRxiv - Immunology 2021Quote: ... Additional to the NF-κB binding motifs NF-κB response element and a TATA-like sequence from pNFκB-Luc vector (Clontech Cat. No. 631904) were added to the Firefly luciferase gene and introduced into pCDH-CMV-EF1-Puro.
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bioRxiv - Plant Biology 2020Quote: ... The genetic regions containing cmaA and surrounding regions were amplified using PCR primer sets (S2 Table) that were designed based on the Pcal ES4326 registered sequence with Prime Star HS DNA polymerase (TaKaRa, Otsu, Japan), then dA was added to the 3’ end of the PCR product with 10 x A-attachment mix (TOYOBO ...
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bioRxiv - Microbiology 2022Quote: ... An internal ribosomal entry site (IRES) was inserted behind the ACE2 sequence via restriction digestion of a pEF1a-IRES vector (Cat. n° 631970, Takara Bio Inc.) with NheI-HF and SalI-HF (NEB) ...
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bioRxiv - Genetics 2019Quote: ... We amplified the fragment by PCR (see Table S3 for the primer sequences) with Tks Gflex™ DNA Polymerase (Takara, Cat#R060A) and purified it ...
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DRT111/SFPS splicing factor controls ABA sensitivity in Arabidopsis seed development and germinationbioRxiv - Plant Biology 2020Quote: ... the coding sequence of DRT111 was cloned into the BamH1 and XhoI restriction sites of pGADT7 vector (Clontech, Mountain View, CA, USA) and the cDNA fragments of SF1 were cloned into the SmaI and SalI sites of pGBKT7 (Clontech ...
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bioRxiv - Pathology 2021Quote: Some samples containing high viral RNA copy numbers were sent for viral sequence analysis by gene analysis services (Takara Bio, Shiga, Japan). The next generation sequencing (NGS ...
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bioRxiv - Genomics 2021Quote: For exogenous MYC expression a synthetic DNA sequence encoding MYC fused to TagBFP via a P2A linker was cloned into the pLVX-TetOne-Puro vector (Takara Bio 631849) and the construct was packaged into a lentiviral vector ...
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bioRxiv - Neuroscience 2021Quote: ... encoding murine preproSST transgene fused with GFP via T2A self-cleavage spacer sequence was prepared by AAVpro purification kit (TaKaRa Bio, Japan) and bilaterally injected into PFC (AP +1.9 mm ...
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bioRxiv - Microbiology 2023Quote: ... the HCoV-OC43 N sequence was cloned into backbone vector pLVX-EF1alpha-2xStrep-IRES-Puro by In-Fusion cloning (Takara Bio, Inc.), obtaining pLVX-EF1alpha-OC43-N-2xStrep-IRES-Puro.
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bioRxiv - Plant Biology 2022Quote: ... the entire ELF3 genomic sequence present in three HIF pairs was amplified using Ex Taq DNA Polymerase (Takara Bio, Kusatu, Shiga, Japan) and 1186 bp of promoter sequence upstream of the ELF3 start codon in HIF pair 10 was amplified using ALLin™ RPH Polymerase (highQu GmbH ...
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bioRxiv - Molecular Biology 2022Quote: ... and amplified by PCR using a forward primer containing a T7 promoter sequence (Supplemental Table 10) with the Advantage HF 2 kit (Takara Bio 639124) and the following cycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: The MDM4 coding sequence (NM_002393.5) was cloned into Lenti-X™ Tet-One™ Inducible Expression System (Takara Bio, cat. no. 631847). The resulting plasmid was then subject to whole-plasmid sequencing for verification ...
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bioRxiv - Evolutionary Biology 2023Quote: The 3’UTR sequence of CcTRPM gene was amplified by the 3’-Full RACE Core Set with PrimeScriptTM RTase kit (Cat# 6106, Takara, Kyoto, Japan). miRNAs for CcTRPM were predicted by a service provider LC science with two software programs of miRanda (http://www.microrna.org ...
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bioRxiv - Microbiology 2023Quote: The whole Gag sequence was amplified from wild-type Gag plasmid by PCR and cloned into pGEX6P1 and pmCherry-C1 (Takara Bio Inc.). Fragments of MAp17 (a.a ...
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bioRxiv - Plant Biology 2023Quote: For Yeast-two-Hybrid (Y2H) experiments coding DNA sequences were cloned into pDONR207 and recombined into pGDAT7® or pGBKT7® vectors (Clontech). Plasmids were transformed into the AH109 yeast strain by the lithium acetate (LiAc ...
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bioRxiv - Plant Biology 2023Quote: The full-length coding sequences (CDS) of NPR1 and TGA were fused to the bait vector pGBKT7 (Clontech, Palo Alto, CA, USA) to generate BD-NPR1 and BD-TGA ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Plant Biology 2023Quote: ... genomic sequences at the length of 700∼2 000 bp were amplified from genomic DNAs with PrimeStar Max DNA polymerase (Takara, cat. R045A). The resulting fragments were cloned into vector backbones derived from pPY22 (mNeonGreen-Hygromycin B ...
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bioRxiv - Bioengineering 2024Quote: ... the target scaffold sequence was first amplified in double-stranded DNA (dsDNA) form via PCR from M13mp18 using PrimeSTAR Max Premix (Takara Bio, R045A) and 0.8 µM primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Cancer Biology 2022Quote: ... After 48 hours supernatants were collected and employed to LAL-B cells in Retronectin (Takara Bionic Otsu, Shiga 520-2193, Japan) pre-coated no tissue culture 24-well plates (Falcon ...
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bioRxiv - Neuroscience 2019Quote: ... The remaining 5′ sequence was obtained by 5′-RACE on medaka brain poly(A)+ RNA using the Marathon cDNA Amplification Kit (Takara Bio, Shiga, Japan), essentially as described previously (Kawabata et al. ...
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bioRxiv - Systems Biology 2019Quote: ... PCR amplification allowed to obtain the coding sequence for human HSF1 that was cloned into peGFP N3 vector (Clontech Laboratories Mountain View, CA); the plasmid was then verified by sequencing (GATC Biotech ...
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bioRxiv - Genetics 2019Quote: ... and performed the 2nd round of PCR to attach to the libraries adaptor and index sequences for the NGS analysis for 8 cycles again with Tks Gflex™ DNA Polymerase (Takara, Cat#R060A). The DNA sequences of the adaptor/index primers are listed in Table S6 ...
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bioRxiv - Microbiology 2019Quote: ... flanked by a 5’ terminal Kozak sequence and 3’ terminal stop codon into the Nhe1 and Sal1 sites of pIRES2-EGFP (Clontech cat # 6029-1).
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bioRxiv - Genetics 2021Quote: ... The codon-optimized DUSP16 ORF sequence was cloned into the lentiviral Lenti-X™ Tet-On® 3G Inducible response vector (Takara Bio) using the GATEWAY® cloning system and a lentiviral stock of this inducible DUSP16 expression plasmid was generated as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... coding sequences were amplified by PCR from zebrafish cDNA using specific primers (Table S1) and subcloned into mCherry-C1 vector (Clontech, Mountain View, CA). hVPS4A in pEGFP-C1 was previously described in Elia et ...
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bioRxiv - Biochemistry 2021Quote: ... In-fusion cloning was used for the ligation of sequence fragments to vector with the In-fusion HD Cloning Kit (Clontech Laboratories, Inc, US). Site-directed mutations were accomplished according to the protocol provided in a Mut Express II Fast Mutagenesis kit V2 (Vazyme ...
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bioRxiv - Genetics 2019Quote: ... and the donor sequence, was sub-cloned and inserted into the vector (pX601, addgene #61591) using a DNA ligation kit (Clontech, Mountain View, CA). A plasmid for mutation replacement genome editing was constructed as described previously15 ...
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bioRxiv - Genetics 2019Quote: ... gRNA-1 target site or gRNA-4 target site and the donor sequence, were sub-cloned and inserted into the vector (pX601, addgene #61591) using a DNA ligation kit (Clontech, Mountain View, CA). To avoid repeated cleavage after mutation replacement ...
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bioRxiv - Microbiology 2022Quote: ... we amplified the gene (ORF2) encoding the VP1 sequence of the HuNoV Sydney strain by PrimeStar HS DNA polymerase (Takara Bio, cat. #r010a) and replaced it with the sequence encoding GFP in the pT7-RRV-NSP3-GFP constructed previously (12 ...
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bioRxiv - Physiology 2022Quote: ... The rat IGF1 coding sequence was then inserted into the linearized scAAV-CMV plasmid using In-Fusion cloning (Takara Bio; Cat. No. 639650). The resulting plasmids for scAAV-CMV-GFP and scAAV-CMV-IGF1 were packaged using AAV2/9 serotype by Vector Biolabs (Malvern ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR-amplified enhancer-containing fragments were tandemly inserted into the upstream region of the original promoter sequence by In-Fusion cloning (TAKARA BIO, Shiga, Japan). This process is summarized in Figure S3 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Site-directed mutagenesis in GC-A and GC-B and construction of chimeric receptors were performed through use of the In-Fusion HD plus cloning kit (Takara Bio Inc.). The primers used are listed in Supplementary Table 1 ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and resultant RNAs (10 ng per sample) were subjected to cDNA library construction (SMARTer Mouse TCR a/b profiling kit, Takara Bio, Japan). The next generation sequencing was performed with MiSeq (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting fragments were cloned into vector backbones derived from pPY22 (mNeonGreen-Hygromycin B) or pPY23 (mNeonGreen-Nourseothricin) using the In-Fusion Snap Assembly Kit (Takara, cat. 638948). For PpREN knockout ...
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bioRxiv - Developmental Biology 2020Quote: ... Expression levels of the stag paralogues (primer sequences in Supplementary Table S3) and haematopoietic markers were measured using SYBR Premix Ex Taq II (Takara Bio Inc., Kusatsu, Japan) on a Roche LightCycler400 ...
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bioRxiv - Molecular Biology 2019Quote: ... This region was PCR amplified with DinoSL and KbrSRP-U6R1 primer set (sequences and Tm in Table 2) using the high fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Shiga Prefecture, Japan) at 94°C for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... 53) and Hif1β shRNA (target sequence: 5’-GGACAGAGATCCAAGGTTT-3’) were cloned into the pSIREN-RetroQ expression vector (631526; Clontech Laboratories Inc., Mountain View, CA). The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011 ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were subcloned in frame with GFP sequence in 3′ into pCS2+-GFP vector using In-Fusion® HD Cloning Kit (Takara Bio USA, Inc.). For rescue experiments ...
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bioRxiv - Biochemistry 2023Quote: ... and the resulting DNA sequences were synthesized (Twist Bioscience, California, USA) and cloned into the pET-28a(+) plasmid using In-Fusion cloning kit (Takara Bio Inc., Shiga, Japan) or the iVEC3 method (58) ...
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bioRxiv - Plant Biology 2023Quote: ... The FW2.2 full-length coding sequence was amplified from cDNAs prepared from tomato (cv. AC) fruits at 5 DPA using PrimeSTAR MAX DNA polymerase (TAKARA BIO Inc., Kusatsu, Japan) and primers including the attB sites (Supplemental Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...