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Citations for Takara Bio :
251 - 300 of 780 citations for Family With Sequence Similarity 46 Member B FAM46B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... following which these sequences were inserted into the parental plasmid using In-Fusion® cloning kit (Takara Bio Inc.).
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bioRxiv - Neuroscience 2023Quote: ... Constructs containing point-mutated ARE-like sequences were prepared using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Because an ARE half-site can function alone to confer androgen inducibility (Pihlajamaa et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the standard phenol-chloroform extract method and subjected to DNase I (Takara, 2270 B) treatment and reverse transcription for DRIPc-seq library construction ...
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bioRxiv - Plant Biology 2020Quote: ... The CDS of PuACO1 was cloned into the KpnI and EcoRI sites downstream of the GFP sequence and the CaMV 35S promoter in the pRI101 vector (TaKaRa). The recombinant Pro35S:Myc-PuBZR1 and Pro35S:GFP-PuACO1 constructs were infiltrated into tobacco (N ...
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bioRxiv - Plant Biology 2019Quote: For Yeast-two-Hybrid (Y2H) assays coding sequences were cloned into pDONR207 and recombined into the pGDAT7 and pGBKT7 (Clontech). Using the co-transformation techniques41 these constructs were transformed into the AH109 strain (Clontech).
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bioRxiv - Genetics 2021Quote: ... Table 1) against two unique sequences in the spCas9 that differs from dCas9GCN4 using Xfect RNA transfection reagent (Takara #631450) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Then the homology arms and fluorescent protein sequences were assembled and cloned into the BamHI site of a pUC19 vector using Gibson assembly (Clontech). The primer sequences for the mGFP-EB1 ...
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bioRxiv - Microbiology 2020Quote: ... DNA sequences that flanked the ittA sRNA locus were amplified using PCR with PrimeSTAR GXL polymerase (Takara, Mountain View, CA). For the upstream fragment ...
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bioRxiv - Developmental Biology 2020Quote: The ERK-KTR-mCherry construct for transient expression was prepared by first inserting the coding sequence for ERK-KTR (Regot et al., 2014) into a CMV-driven mCitrine C1 expression vector (TaKaRa), and then replacing the fluorophore for mCherry ...
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bioRxiv - Molecular Biology 2021Quote: ... Gal4-VP16-human SREBP-1c plasmid was prepared by insertion of a VP16-transactivation domain fused to a human SREBP-1c fragment from the 431st amino acid to the C-terminus (amino acids 431-1123) downstream of the Gal4-DNA binding domain sequence in pM vector (Clontech). The Gal4-RE-Luc plasmid and luciferase plasmid including sterol response element (SRE-Luc ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA fragment of human METTL18 with a C-terminal HA sequence was cloned into AgeI and EcoRI sites of the pQCXIP vector (Clontech). To generate hMETTL18-Asp193Lys-Gly195Arg-Gly197Arg-HA ...
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bioRxiv - Molecular Biology 2020Quote: ... To generate the light-inducible clustering constructs, the CRY2olig sequence (Taslimi et al., 2014a) was inserted with a c-terminal mCherry tag (Clontech) into pGEMHE to generate pGEMHE-CRY2olig-mCherry ...
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bioRxiv - Molecular Biology 2020Quote: ... the coding sequence for Map2 was inserted into the backbone of pEGFP-Tub (BD Biosciences Clontech, Franklin Lakes, NJ, USA) with restriction enzymes XhoI and BamHI after amplification from pDONR223-MAP240 (forward primer ...
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bioRxiv - Plant Biology 2019Quote: ... The JAZ coding sequences were ligated into the multi-cloning site of the Y2H vector pB42AD (Clontech, Mountain View, CA) to generate N-terminal fusions to the B42 transcriptional activation domain ...
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bioRxiv - Cell Biology 2019Quote: A Tmem98-AcGFP fusion construct was generated by cloning the Tmem98 coding sequence into the SmaI cut pAc-GFP-N2 vector (Clontech). TMEM98-AcGFP did not inhibit FRAT2 activity to a similar extent as TMEM98-FLAG (data not shown) ...
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bioRxiv - Biophysics 2019Quote: ... and the full mNeonGreen coding sequence was inserted in its place using In-Fusion cloning (Takara Bio; Mountain View, CA). NG-Scarlet and NG-Cherry were created by deleting mRuby3 from NG-Ruby3 by inverse PCR and insertion of either mScarlet-I or mCherry using In-Fusion cloning (Takara Bio) ...
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bioRxiv - Microbiology 2019Quote: ... Primers P5 and P6 were annealed to create a dsDNA molecule encoding the sgRNA sequence with 5’ and 3’ extensions to enable InFusion Cloning (Clontech) into BtgZI-digested pL6 ...
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bioRxiv - Microbiology 2019Quote: ... A plasmid encoding a gene fusion between the 5‘-201 nucleotides of PR8 segment 5 and GFP was made by PCR-cloning the appropriate IAV sequence into pEGFP-1 (Clontech), followed by oligonucleotide-directed PCR mutagenesis (using standard protocols ...
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bioRxiv - Biochemistry 2019Quote: ... The ERE sequences of URAT1/SLC22A12 promoter region was amplified to get mutants using PrimeSTAR® Mutagenesis Basal Kit (Takara) with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... The vimentin-null mEFs expressing vimentin are created by PCR amplification of the vimentin coding sequence using CloneAmp polymerase (Clontech) from pcDNA4-vimentin (provided by J ...
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bioRxiv - Neuroscience 2021Quote: ... IGF1R-mEGFP was prepared by inserting the coding sequence of IGF1R (a gift from Dr. Inna Slutsky) into pEGFP-N1 (Clontech) containing the A206K monomeric mutation in EGFP and the CAG promoter ...
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bioRxiv - Cell Biology 2021Quote: ... Constructs coding for FRB (DmrA) and FKBP (DmrC) sequences were obtained from ARIAD Pharmaceuticals and are now available from Takara Bio Inc ...
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bioRxiv - Cell Biology 2021Quote: The FKBP sequence was tagged to a 3xFLAG-LRRK2 vector using IN-FUSION HD cloning technology (Clontech, Takara, cat #638920). LYSO-LRRK2 was created by adding the N terminal domain of the LAMTOR1 sequence (aa 1-39 ...
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bioRxiv - Cell Biology 2021Quote: The FKBP sequence was tagged to a 3xFLAG-LRRK2 vector using IN-FUSION HD cloning technology (Clontech, Takara, cat #638920). LYSO-LRRK2 was created by adding the N terminal domain of the LAMTOR1 sequence (aa 1-39 ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Microbiology 2020Quote: ... Dengue NS5-GFP fusion construct was generated by inserting the dengue New Guinea C NS5 coding sequence from pDVW601 (44) into pACGFP1-N1 (Clontech) as previously described (20).
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bioRxiv - Plant Biology 2020Quote: A reporter plasmid was made by cloning the 76bp AG intron sequence upstream of the lacZ gene in the vector pLacZi (Clontech). The yeast reporter strain was made by integration of the linearized AG intron reporter plasmid into the yeast strain YM4271 ...
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bioRxiv - Plant Biology 2021Quote: ... The EVD coding sequence (At5TE20395) was directly amplified from wt Col DNA using primers containing appropriate plasmid homology for In-Fusion Cloning (Clontech) into their respective digested binary vector backbones ...
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bioRxiv - Microbiology 2022Quote: Sequence of the human variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) using LA Taq polymerase (TaKaRa-Bio, Inc., Kusatsu, Japan) for Illumina MiSeq paired-end sequencing ...
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bioRxiv - Microbiology 2020Quote: ... overhangs upstream and downstream of the gene of interest were amplified (primers sequences available in Table S1) and purified then cloned into linearized pCM433 using In-Fusion HD Cloning plus kit (Takara), primer design was done using primer design tool (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse primers carrying the above restriction sites were designed to amplify a 3kb fragment from Diaph2 MGC human cDNA clone (Dharmacon) and the amplicon encompassing the entire cDNA sequence minus the DAD domain was cloned into pLVX-mCherry-N1 (Clontech) to generate lentiviral expression vector pLVXdeltaDADmcherry ...
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bioRxiv - Developmental Biology 2021Quote: ... was constructed by inserting a gBlock encoding sfGFP followed by an SV40 polyA sequence and a FRT-flanked ampicillin cassette in place of EGFP in the pEGFP-C1 plasmid (Clontech). For kcne4 and ndufa4l2a BACs ...
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bioRxiv - Cancer Biology 2020Quote: ... overhangs (see table for primer sequences) and fused in frame at its carboxyl end via GA to eGFP in the vector eGFP-N3 (CLONTECH), to generate flZEB1-GFP ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... they were cloned into a SmaI site located downstream of the TRE sequence in pLenti6PW–TRE using an In–Fusion Cloning Kit (Clontech)(Egashira et al ...
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bioRxiv - Molecular Biology 2022Quote: ... These fragments were combined to amplify the full genomic sequence that was cloned into a pENTR/D plasmid by InFusion (Takara) to generate pENTR-gMINU2 ...
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bioRxiv - Microbiology 2022Quote: ... gRNAs targeting the selected region of the CMV and bGH sequences (235-310 and 1917-2029, respectively in pEGFP-N3, Takara), were designed accordantly to each ROI (Table S3 and Supplementary Methods) ...
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bioRxiv - Molecular Biology 2019Quote: ... The N-terminal 3X-FLAG-tagged ThPOK expression plasmid was generated by cloning the ThPOK coding DNA sequence (CDS) in the backbone of pEGFPC-3 (Clontech) plasmid after replacing the CDS of EGFP with 3X-FLAG ...
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bioRxiv - Cancer Biology 2019Quote: ... was excised and inserted in frame into a pcDNA3 vector containing fireflluciferase cDNA followed by a T2A sequence using the Infusion HD-eco dry kit (Clontech), as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The mRFP fused FUST-1 cDNA plasmids were used to generate cDNA only plasmids for splicing reporter rescue by removing the mRFP sequences using In-Fusion (Takara). Splicing reporter of fust-1 exon 5 was prepared by cloning exon 4 to exon 6 into the plasmids provided by Dr ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Molecular Biology 2021Quote: ... A 150-250 bp region encompassing each targeted locus was PCR amplified from ∼40 ng genomic DNA with ends containing partial Illumina adapter sequences using CloneAmp HiFi PCR (Takara). Reaction conditions were as follows ...
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bioRxiv - Genetics 2020Quote: ... and complementary primer pairs (sequences available on request) was used to generate TMEM127 variants in the pEGFP-C2 (Clontech Laboratories) and pCMV6-XL5 (Origene ...
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bioRxiv - Plant Biology 2021Quote: ... MEL1 and lacZ) under distinct GAL4 upstream activating sequences as described in Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual (Clontech). The transformants were grown on synthetic dropout (SD ...
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bioRxiv - Cell Biology 2019Quote: ... The sequences encoding murine talin1 head domain (THD, aa 1-433) or full-length talin1 were cloned into pEGFP-N1 (Clontech). The sequences encoding human Rap1a(Q63E ...
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bioRxiv - Neuroscience 2021Quote: ... together with the P2A (Donnelly et al., 2001) sequence ATNFSLLKQAGDVEENPGP into the modified pEGFP-C1 vector by replacing EGFP (Clontech).
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bioRxiv - Evolutionary Biology 2021Quote: ... respectively) and an mCherry coding sequence were amplified from the template plasmid by PCR using PrimeSTAR GLX DNA polymerase (TAKARA) along with the sense and antisense primer pair (5’-ATTACCTGGGGGTATCCCCTT-3’ and 5’-CACTCTTCTGGTTGTGGTTGC-3’) ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-026 was generated by subcloning a DNA fragment encoding an N-terminus FLAG-tagged mouse eIF2α coding sequence flanked by BamHI and EcoRI sites into the BglII and EcoRI sites of pLPCX (Clontech) using standard molecular biology methods ...