Labshake search
Citations for Takara Bio :
3851 - 3900 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Genomics 2021Quote: ... Libraries from 4-cell embryos were also prepared using 18-30 nt gel purified RNA using the SMARTer smRNA-Seq Kit (Clontech) and NEXTflex-Small-RNA-Seq (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The concentrations of the host and the parasitic RNAs were measured by RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with sequence-specific primers (Supplementary text).
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bioRxiv - Genetics 2020Quote: ... The products obtained were subsequently subjected to semi-nested PCR with a LA Taq Hot Start polymerase kit (TaKaRa Biotechnology). The first round of amplification was performed with primers ...
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bioRxiv - Neuroscience 2021Quote: Total RNAs from the mouse forebrain were reverse transcribed using the PrimeScript II first strand cDNA Synthesis Kit (Takara Bio) with the random hexamer primer ...
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bioRxiv - Immunology 2020Quote: ... 10ng of RNA was used to prepare bulk TCR-seq libraries using the SMARTer Mouse TCR a/b Profiling Kit (Takara) according to instructions ...
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bioRxiv - Immunology 2021Quote: ... 293T Lenti-X cells were seeded onto 10-cm Petri dishes and transiently transfected (CalPhos™ Mammalian Transfection Kit, Clontech Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the absence of unwanted substitutions.
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bioRxiv - Plant Biology 2023Quote: ... the promoter fragment was amplified and cloned between HindIII and XbaI sites using In-Fusion® HD Cloning Kit (Takara), generating pGWB502-HSP90.7pro ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... was derived from foreskin-fibroblasts using the Stemgent mRNA Reprogramming Kit (Stemgent) as described previously (40) and maintained in a feeder-free culturing system Cellartis DEF-CS 500 (Takara). The inducible SpCas9 cell line was generated as described earlier (41).
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed using first-strand cDNA and a primer pair designed for target genes using the SYBR Premix Ex Taq Perfect Real Time Kit Tli RNAaseH Plus (TAKARA) and Thermal Cycler Dice Real Time System (Model TP800 ...
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bioRxiv - Cell Biology 2023Quote: AAV plasmids were prepared by subcloning the ORF to pAAV-CAG-DNP63A7 or using the In-Fusion HD Cloning kit (Clontech).
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and was further reverse-transcribed into cDNA through the PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa). A LightCycler 480 II (Roche Applied Science ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was then cloned into the EcoRI and NotI recognition site of the expression plasmid pCAG neo using HD Cloning Kit (TaKaRa). To construct deletion mutants of bovine hnRNPM ...
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bioRxiv - Microbiology 2023Quote: ... The resultant reaction mixture was then 10-fold diluted and subjected to quantitative PCR using TB Green Premix Ex Taq II kit (TaKaRa) in combination with a tag primer (no ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription reaction was set up using the cleaned-up RNA (1.6µg) using PrimeScript™ RT reagent Kit (Takara, Japan). The RT reaction was carried out with buffering temperature at 25 ºC for 2 mins ...
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bioRxiv - Immunology 2022Quote: FACS sorted cells (5,000 cells) were subjected to cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech). Barcoded libraries were generated by the Nextera XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Immunology 2022Quote: FACS sorted cells (5,000 cells) were subjected to cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech). Barcoded libraries were generated by the Nextera XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... n = 3 pools of 250 GFP+ cells were collected for direct RNA-Seq library preparation using the SMART-Seq HT PLUS kit (Takara), according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from PAMs using the TRIzol reagent and was reverse transcribed using the PrimeScript RT kit (TaKaRa). qPCR was performed using the PowerUp SYBR Green Master Mix on the ABI StepOnePlus system ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were ligated into pACT7_SC14 (HBB minigene reporter as previously described (31) using homology-based cloning technology (In-Fusion HD Cloning kit, Takara Bio). Following sequence verification ...
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bioRxiv - Plant Biology 2023Quote: ... The tissue was fixed for 30 min and the immunoprecipitation performed using a SEP3-specific antibody followed by library preparation using ThruPLEX DNA-Seq Kit (Takara) and deep sequencing65 ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then used for library preparation using the SMARTer small RNA (smRNA)-seq kit for Illumina (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
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bioRxiv - Molecular Biology 2023Quote: ... the 3’ UTR of the virus were determined using the SMARTer®RACE 5’/3’ Kit (Takara Bio, Kusatsu, Japan). 2.4 µg of RGMoV gRNA was polyadenylated using Poly(A)polymerase (600 u/µl ...
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bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... the supernatants of infected cells were collected and infectious virus particles were measured using the Adeno X Rapid Titration Kit (Takara Bio Europe ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA was subjected to reverse transcription to generate RNA cDNA using the widely recognized PrimeScriptRT kit (Takara, Kyoto, Japan) provided by the esteemed Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified and ligated into the pMD-20 vector or the pANT vector using the Mighty TA- cloning Kit (Takara) or TA-Enhancer Cloning Kit (Nippon Gene ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized from 10 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was inserted in between the KpnI and HindIII sites of the pOPINK vector (42) using the In-Fusion HD Cloning Kit (Takara). The pOPINK vector incorporates an N-terminal GST tag and a 3C protease cleavage site into the PhPINK1 construct ...
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bioRxiv - Developmental Biology 2023Quote: ... The yeast two-hybrid library was generated from the poly(A)+ mRNA isolated from mixed-stage N2 nematodes using the Matchmaker kit (Clontech) as per the manufacturer’s protocol and transformed into Y1HGold strain (MATα ...
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bioRxiv - Cancer Biology 2023Quote: ... forward-Trail and reverse-Trail were generated in PZH vector amplified and extracted using In-Fusion HD Cloning kit (Clontech). Plasmids were sent for Sanger sequencing with reverse and forward primer to control any mutation point ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA was prepared using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, CA, USA). Libraries were prepared using a Nextera XT DNA Library Prep Kit (Illumine ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of 5’ linker (10 μM) was ligated with 10 μl of Mighty mix (DNA Ligation Kit
, catalog num. 6023, Takara) to 4 μl of the sample coming from the previous step ... -
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT reagent kit with gDNA Eraser and TB Green® Premix Ex TaqTM were purchased from Takara (Otsu, Japan). Molecular Probes™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ...
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bioRxiv - Immunology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the RNA concentration was determined via Nanodrop and up to 100 ng was used as an input material for library preparation using the Clontech Ultra Low kit (Clontech).
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bioRxiv - Genomics 2023Quote: ... 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Afterwards an average of 10 fmol of amplified cDNA was used to prepare library following SQK-PBK004 kit (PCR Barcoding kit ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing libraries were prepared by Edinburgh Genomics using a SMART-Seq® v4 PLUS RNA Kit (Takara Bio USA) with 9 cycles of amplification ...
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bioRxiv - Neuroscience 2022Quote: ... the BamHI/BsrGI digested PCR product was ligated to the linearized backbone using the DNA Ligation Kit - Mighty Mix (TaKaRa) resulting pPB-ef1a-NLS-EGFP-T2A-puro.
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bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.