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Citations for Takara Bio :
3751 - 3800 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... they were cloned into a SmaI site located downstream of the TRE sequence in pLenti6PW–TRE using an In–Fusion Cloning Kit (Clontech)(Egashira et al ...
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bioRxiv - Biophysics 2021Quote: ... The resulting DNA fragments were gel-extracted and cloned individually into the pBADC3 plasmid using InFusion EcoDry cloning kit (TaKaRa). All genes were fused with a C-terminal StrepII-tag for affinity purification.
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bioRxiv - Neuroscience 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 ξ 150 bp pair-end read configuration to a minimum depth of 40 million reads per sample ...
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bioRxiv - Molecular Biology 2022Quote: Rps29 5’UTR and coding region cDNAs were synthesised using SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc. USA). PCR was performed using gene-specific primers (40S 5’ RACE R ...
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bioRxiv - Developmental Biology 2022Quote: ... The picked endogenous IHCs and iOHCs were immediately subjected to reverse-transcription and cDNA amplification using a Smart-Seq HT kit (Cat# 634437, Takara). The post-amplified cDNA (1 ng ...
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bioRxiv - Genetics 2022Quote: ... The sequence of amplified MYSM1 cDNA was verified by Sanger sequencing before subcloning into the pLVX overexpression vector using the In-Fusion cloning kit (Takara). Q5 site directed mutagenesis kit was used to make H656R mutant.
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step) were finally processed into sequencing libraries using PrepX DNA Library Kit (400075, Takara) and sequenced using the Illumina platform (NextSeq 500 ...
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bioRxiv - Microbiology 2022Quote: ... These two amplified fragments were inserted into the pBh-EPS vector [46] at the PstI site using the In-FusionHD Cloning Kit (Takara). The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega) ...
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bioRxiv - Neuroscience 2022Quote: The genomic location of the insertion in the enhancer-trap line Et(gata2:EGFP)bi105 was mapped using a linker-mediated approach with the Universal Genome Walker™ 2.0 Kit (Clontech). A detailed user manual is available online ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from mouse cochleae was extracted using an RNA Extraction Kit according to the manufacturer’s instructions (#9767, TaKaRa, Japan). The extracted RNA concentration was measured using a Nanodrop 2000 spectrophotometer (#ND-LITE ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RNA quantification was performed by RT-qPCR targeting the S gene of SARS-CoV-2 using One Step PrimeScript RT-PCR Kit (Takara) with the following SARS-CoV-2 specific primers and probes ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription was performed using the oligo(dT) primer and avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA preamplification was performed using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech) according to Fluidigm’s manual for mRNA sequencing on the C1 system ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was prepared from cortical samples from control and cKO animals (3 animals/group) by using RNAiso-plus kit (Takara) and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers containing 15 bp tails homologous to cloning sites within the pGL4.10[luc2] vector which is necessary for cloning using the In-Fusion® HD cloning kit (Takara). Primer sequences are indicated in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 ng RNA was reverse transcribed into complementary DNA (cDNA) using a PrimeScript II 1st Strand cDNA Synthesis kit (TaKaRa) or a QuantiNova Reverse Transcript Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
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bioRxiv - Physiology 2022Quote: ... and cDNA libraries were generated using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio, Inc., Kusatsu, Japan). Libraries were high-output single-end sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Pathology 2020Quote: ... 3’-RACE-PCR was performed as per the instructions of SMARTer™ RACE-cDNA Amplification Kit User Manual (Clontech, 2012) using primers given in Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from E8.5 trunk tissue (two wild-type trunks and two Aldh1a2-/- trunks) and DNA sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian (Takara). Sequencing was performed on Illumina NextSeq 500 ...
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bioRxiv - Plant Biology 2020Quote: ... Total amount of 1 μg RNA was used as template for first-strand cDNA synthesis with the oligo(dT) primer and the PrimeScriptTMRT reagent Kit with gDNA Eraser (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Around 1μg RNA was taken for cDNA preparation by employing PrimeScript 1st strand cDNA Synthesis Kit (Clonetech Takara Bio, USA) (Chowrasia et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... 700pg of total RNA were used for cDNA amplification with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... Both CnAOEC and ISO-HAS-B were cultured in Endothelial Cell Growth Medium 2 Kit (Takara Bio, Inc. Kusatsu, Japan). All cells used were routinely tested for Mycoplasma using PCR and were submitted to ICLAS Monitoring Center (Kawasaki ...
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bioRxiv - Bioengineering 2020Quote: ... Ligation products with the correct size were purified by DNA agarose gel extraction using NucleoSpin Gel and PCR Clean-Up Kit (Takara, 740609.250 ...
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bioRxiv - Pathology 2020Quote: ... and then 8μL of each was reverse transcribed into cDNA by using the commercial company's reverse transcription kit (TAKARA No. 6110A). The full-length spike gene (3882 nt ...
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bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was then performed using the oligo (dT) primer and Avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). We conducted RT-PCR using KOD One (TOYOBO ...
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bioRxiv - Genetics 2021Quote: ... fust-1 genomic fragment containing sequences from 2181bp upstream ATG to just before stop codon was cloned into the Sma I site of pHK-mRFP vector in frame with mRFP by In-Fusion HD Cloning Kit (Takara). This plasmid was further used to generate backbone structure containing fust-1 promoter and mRFP ...
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bioRxiv - Microbiology 2021Quote: ... extracted RNA was reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The gene quantitative PCR was performed using TB green Premix EX Taq (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Plant Biology 2021Quote: ... was added to mitigate DNA contamination prior to first-strand cDNA synthesis with PrimeScript™ RT reagent kit (Takara Bio) from approximately 1 g total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... Ten nanograms of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... pnYK vector was created from pnYC vector (58) by homologous recombination between the chloramphenicol and kanamycin genes resistance gene using the In-Fusion kit (Clontech) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Two-point five microliters of the polyadenylated sample was used for cDNA synthesis by the PrimeScript RT reagent kit with gDNA eraser (TaKaRa), with the y625 primer in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Microbiology 2020Quote: ... Approximately 1,000 ng of RNA was reverse transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... For analysis of the NAD metabolome HEK293T cells were transfected with 1µg of pEGFP-C1 Empty Vector or pEGFP-C1-CMV-PARP10 using CalPhos Mammalian Transfection Kit (Takara Bio). 6 hours later the cells were treated with chemical treatments in DMEM + 10% FBS at 37°C 5% CO2 for 18h.
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bioRxiv - Synthetic Biology 2020Quote: ... The amplified fragments were gel-purified and fused using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). Full sequences of the plasmids used in this study are provided in Supplementary Information I ...
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bioRxiv - Bioengineering 2020Quote: ... and recovery of ribosome footprints were performed as described previously.[55] Footprints were dephosphorylated and cDNA libraries were prepared using the SMARTer smRNA-Seq Kit (Clontech), with an additional rRNA depletion step prior to PCR amplification ...
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bioRxiv - Pathology 2020Quote: ... Single stranded cDNA was synthesized with the oligo(dT) primer using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), the obtained cDNAs were analyzed by real-time PCR ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: Four hundred ng total RNA was employed for cDNA synthesis using ProtoScript II First Strand cDNA Synthesis kit(TaKaRa,#RR036A) according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was generated through a 3’-RACE-Ready cDNA synthesis reaction using the SMARTer RACE cDNA amplification kit (Clontech), following the user manual ...
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bioRxiv - Developmental Biology 2020Quote: ... and then further into the ClaI site of the avian replication-competent retrovirus vector RCASBP(B) (Li, Monckton, & Godbout, 2014) with the In-Fusion HD cloning kit (Clontech). The mutant construct that converts glutamine (Q ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Forward (control) and reverse (target) RNA probes were synthesized from plasmids amplified via PCR (Advantage HD Polymerase Kit, Takara Bio) using T7 or SP6 RNA Polymerases (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Microglial RNA was isolated using the TRIzol method and cDNA libraries were generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Components (Takara) according to the manufacturer’s protocol.The libraries were sequenced as 150 bp paired-end reads with an average read depth of 42 million (range 16-98M ...
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bioRxiv - Genetics 2020Quote: ... The guide for Cas9 was obtained by direct hybridization of the oligos and inserted in the same plasmid by Gibson assembly (In-Fusion HD cloning kit; Clontech) using the BtgZI sites.