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Citations for Takara Bio :
4051 - 4100 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... ∼0.37 pg) per tube—was subjected to amplification using a PicoPLEX® Gold Single Cell DNA-Seq Kit (Takara Bio) and indexing primers (DNA HT Dual Index kit ...
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bioRxiv - Systems Biology 2024Quote: ... in conjunction with the Mir-X™ miRNA qRT-PCR TB Green® Kit from TAKARA (San Jose, CA, USA). After confirming miRNA inhibition in tick tissues ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on ABI QuantStudio 6 Flex Real-Time PCR System using the SYBR Premix ExTaq kit (Takara) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription and cDNA amplification were performed with SMART-Seq™ v4 Ultra™ Low Input RNA Kit (Clontech, 634890), followed by cDNA fragmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... then we reversed transcription of these RNA to create cDNA by a PrimeScript 1st strand cDNA synthesis kit (Takara, Japan). FMNL2-EGFP vector was generated by Wuhan GeneCreate Biological Engineering Co ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA was reverse transcribed into complementary DNA by PrimeScript RT (reverse transcriptase) using a primeScript RT-PCR kit (Takara), and qPCR was performed with SYBR Green-Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of total RNA was amplified and converted into cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). About 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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bioRxiv - Molecular Biology 2024Quote: ... The rRNA-removed RNA was used to construct barcoded RNA-seq libraries using the SMARTer Stranded RNA-Seq Kit (Takara).
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bioRxiv - Microbiology 2024Quote: ... the extracted RNAs were used to synthesize cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (TAKARA, Beijing, China). The synthesized cDNA served as the template for determining the transcriptional levels of selected tight junctions (ZO-1 ...
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bioRxiv - Molecular Biology 2024Quote: The over-expressed protein of interest was purified using His60 Ni superflow resin and gravity column kit (Clontech Laboratories Inc.). The His60 Ni Gravity column (1 mL resin ...
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bioRxiv - Microbiology 2024Quote: ... Routine cloning was performed using the In-Fusion HD Cloning Plus kit (Takara Bio USA, Inc., Mountain View, CA, USA). Routine and high-fidelity PCR amplifications were performed using RedTaq (Denville Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 ng-1 ug of mRNA was reverse transcribed to cDNA using the PrimeScript RT Master Mix kit (Takara, RR036A). Real-time PCR was performed using the TB Green Premix Ex Taq kit (Takara ...
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bioRxiv - Microbiology 2024Quote: ... and cloned by homologous recombination into the appropriate recipient parental backbone vector using the In-Fusion HD cloning kit (Clontech). The ligation product was transformed into DH5〈 or SURE2 competent cells ...
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bioRxiv - Immunology 2024Quote: ... The individual 25 µl volume 5’RACE PCR reactions were then carried out in 96-well plates using the thermocycler program recommended by the 5’RACE kit (Takara), with 35 cycles of gene-specific amplification with an annealing temperature of 60°C ...
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bioRxiv - Microbiology 2024Quote: ... The presence of integrated transgenic DNA in the PfATP2-HAreg and PfATP2-GFPreg parasites was determined by PCR from total parasite genomic DNA (extracted from isolated trophozoite-stage parasites (see below)) using a QIAGEN DNeasy Plant Kit) using PrimerSTAR GXL DNA polymerase (TAKARA). All primers used for PCRs and sequencing are listed in Table S1 ...
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bioRxiv - Immunology 2024Quote: ... Total RNA (4 ng/sample) was processed using the SMARTer Stranded Total RNA-seq Kit v3-Pico Input Mammalian (Takara). Final libraries were quantified with a Tapestation (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... pLV.shRegγ) along with the helper vectors psPAX2 and pMD2.G using the Calcium Phosphate Mammalian Transfection Kit (631312-Takara bio). Lentivirus-containing supernatants were collected 48 hours post-transfection and used to infect target cells in the presence of 10 mg/ml polybrene ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysis was quantified by measuring the lactate dehydrogenase (LDH) amount in the cell supernatant using the LDH cytotoxicity kit (Takara, Clontech) according to the manufacturer’s instructions and expressed as a percentage of total LDH release ...
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bioRxiv - Plant Biology 2024Quote: ... respectively) were inserted into the KpnI and XbaI sites of the vector using the In-Fusion HD Cloning Kit (TaKaRa).
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bioRxiv - Immunology 2024Quote: ... 100ng of total RNA was used for cDNA synthesis by RNA to cDNA EcoDry™ Premix (Double Primed) kit (Takara), and qPCR was performed by Taqman primers with TaqMan™ Fast Advanced Master Mix (Applied Biosystems™ ...
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bioRxiv - Bioengineering 2024Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and library preparations were performed by the Genomics Core using the SMARTv4 for ultra-low RNA input and Nextera XT kits (Takara). Sequencing was also performed on an Illumina NextSeq 2000 sequencing system with paired-end ...
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara), and the following primers ...
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bioRxiv - Cell Biology 2024Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 µg of total RNA was DNase treated and reverse transcribed using the PrimeScript RT reagent kit with gDNA eraser (Perfect real time) (TaKaRa) in a final volume of 20 µl and one microlitre of cDNA was used for subsequent amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus ...
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bioRxiv - Neuroscience 2024Quote: ... A construct lacking the REST binding motif was generated with the use of a PrimeSTAR Mutagenesis Basal Kit (Takara Bio).
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bioRxiv - Molecular Biology 2024Quote: cDNA was synthesized from 10 ng of high-quality RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). Genome coordinates for alternative exons and PCR primers for amplifying cDNA are listed in in Sup Table 11 ...
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bioRxiv - Neuroscience 2024Quote: ... were designed using Primer3Plus (https://www.primer3plus.com/) and sequence accuracy was confirmed by SMARTer RACE 5’/3’ Kit (TaKaRa, Kusatsu, Japan) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... a DNA fragment library of 220-bp insert size was prepared using the PrepX ILM 32i DNA Library Kit (Takara), and mate-pair libraries of 3-kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat ...
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bioRxiv - Neuroscience 2024Quote: ... and a single transmembrane region from human IL2RA (UniProt accession number: P01589) were fused to the N-terminus of KCNQ2 or KCNQ3 using the In-Fusion HD Cloning Kit (Takara). Site-directed mutations were introduced into KCNQ2 and KCNQ3 by standard PCR techniques using PrimeSTAR Max DNA Polymerase (Takara) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RNA copies of SARS-CoV-2 was quantified by RT-qPCR targeting the S gene using the One Step PrimeScript RT-PCR Kit (RR057B, Takara) with specific primers and probes ...
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bioRxiv - Immunology 2024Quote: ... or from sort-purified CD11c+/- splenocytes from Smc3ΔDC mice (for confirming recombination of floxed Smc3 allele by PCR) using the NucleoSpin Tissue kit (Takara Bio) according to the manufacturer protocol.
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bioRxiv - Biochemistry 2024Quote: ... a WT Rep sequence was cloned into the pTD68/6xHis-SUMO expression vector as a geneblock (IDT) with the In-Fusion HD Cloning Kit (Takara) using BamHI and XhoI restriction sites (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nine sequence-modified pET-aaRSs were prepared by PCR amplification followed by self-ligation using a mutated and In-Fusion cloning Kit (TaKaRa). The circular DNA encoding the phi29 DNA polymerase used in the aaRS activity assay (Fig ...
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bioRxiv - Systems Biology 2024Quote: ... The integration of the cassette into pMW219 was performed using the In-Fusion HD cloning kit (Takara Bio, Japan, 639648).
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bioRxiv - Biochemistry 2024Quote: ... and the complementary sense and antisense oligos for each sgRNA were annealed and ligated into the AflII site of the pAIO3 plasmid using an In-Fusion cloning kit (Takara). Before transfection ...
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bioRxiv - Genetics 2024Quote: ... The DNA was purified and concentrated from agarose gels using the Nucleospin Gel and PCR Clean-up kit (Takara Bio) and samples were normalized to 1ng/μl using a Qubit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified and 10ng was used as input into the SMARTer Stranded Total RNA-Seq Kit v3-Pico (Takara). Libraries from the total input RNA ...
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bioRxiv - Immunology 2024Quote: ... cDNA library was generated from 2 nanograms of total RNA using Smart-Seq V4 Ultra Low Input RNA Kit (Takara). 150 picograms of cDNA were used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Biophysics 2024Quote: ... digested with HindIII and EcoRI or the pMT vector (Ridge and Abdulaev, 2000) digested with EcoRI and NotI using In-Fusion HD cloning kit (TAKARA). The plasmids (15 µg per 100 mm culture dish ...
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bioRxiv - Cancer Biology 2024Quote: ... fragments were cloned using the TOPO™ TA Cloning™ Kit and shotgun cloned into Stellar™ Competent Cells (TaKaRa). Individual colonies were picked into 96 well plate culture blocks ...
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bioRxiv - Cancer Biology 2024Quote: ... Ultralow input RNA-seq was performed using the SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Clontech, 634888) and Nextera XT DNA Library Preparation Guide (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... PCR-amplified RABs were then cloned in-frame with MYC:APEX2 through Gibson ligation using the In-Fusion HD kit (639650; Takara Bio) in a modified pGLAP1 vector49 to yield the MYC:APEX2:RABs expression vector ...
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bioRxiv - Cell Biology 2024Quote: ... then collected 2-3 days later for purification with the Adeno-X Maxi Purification kit (Takara Bio, Catalog No. 631533).
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bioRxiv - Cell Biology 2024Quote: ... then collected 2-3 days later for purification with the Adeno-X Maxi Purification kit (Takara Bio, Catalog No. 631533).
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bioRxiv - Biochemistry 2024Quote: ... were obtained by gene synthesis (IDT) and cloned into pOPIN-S vector 36 using the In-Fusion HD Cloning Kit (Takara Clontech). SnJOS2 was amplified from S ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting double-stranded fragments were subcloned at the BsaI site of the pMpGE_En03 vector (Sugano et al., 2018) using the DNA ligation kit Ver.2.1 (Takara Bio, Japan) according to the manufacturer’s instructions ...
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Hydrothermally treated yeast cell wall suppresses the growth of MCF7 cells under isolated conditionsbioRxiv - Biochemistry 2024Quote: ... Regular testing of mycoplasma contamination was performed in the cell lines using CycleavePCR™ Mycoplasma Detection Kit (Takara, Shiga Japan), and only mycoplasma free cells were used for experiments.