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Citations for Takara Bio :
4101 - 4150 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... BAC DNA or hL1-5’_3.3kb plasmids were purified using the NucleoBond® Xtra Midi Plus EF kit (Takara # 740422.50) following instructions for low-copy or high-copy plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... The undifferentiated spermatogonia of meiocmo/mo;sox17::egfp and sox17::egfp wildtype were sorted using JSAN cell sorter (Bay Bioscience) and used for bisulfite conversion using MethylEasy Xceed Rapid DNA Bisulfite modification kit (Takara). The tandem repeat region of 45S-S rDNA IGS was amplified by PCR with EpiTaq HS (Takara ...
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bioRxiv - Evolutionary Biology 2024Quote: ... following the manufacturer’s instructions and were reverse transcribed into cDNA using the PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara). RT‒qPCR analysis of bcd cDNA and its targets was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: Site-directed mutagenesis was carried out either using Q5 site-directed mutagenesis kit (New England Bio) or In-fusion cloning (Takara) according to the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... The EF1α cis-element was switched to that of CiNut (Kitaura et al., 2007) with an In-Fusion Cloning Kit (Clontech) according to the previous reports (Sasakura et al. ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from plant samples with Purelink (Nacalai Tesque, Japan) and reverse transcribed with PrimeScript Reagent Kit Perfect Real Time (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... HSP70-F2/R2 primers (Table S1) and ligated into pBS-SK+ using the In-fusion HD Cloning kit (Clontech, Beijing, China), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
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bioRxiv - Cell Biology 2020Quote: ... 10ng of each RNA isolate was then prepared using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara, 634412). Transcripts were fragmented for 3.5 minutes and amplified for 12 cycles ...
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bioRxiv - Immunology 2021Quote: ... DNase-treated total RNA was used to prepare cDNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio, Mountain View, CA). Libraries were sequenced as 50 bp single-end reads on an Illumina HiSeq sequencer ...
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bioRxiv - Genetics 2021Quote: ... The cDNA samples were prepared from 1 μL total RNA per reaction using a reverse transcription kit (Takara Biotechnology, Tokyo, Japan) and stored at - 20°C for qRT-PCR assays ...
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bioRxiv - Genetics 2021Quote: Adult ovary total RNA was used to amplify the 5’ and 3’ UTR of Pxmeiw68 and PxnanosP using the SMARTer RACE 5’/3’ Kit (Takara, Japan). 5’ and 3’ regulatory sequences were then cloned from 4th instar larval gDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 ng of RNA was used for cDNA synthesis (SMART-Seq v4 Ultra Low Input RNA kit – Takara Bio: #634898). Verification of library quality and sequencing were done as for the embryonic dataset (see above) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and was converted into cDNA prior to sequencing using the SMARTer technology (SMARTer Pico PCR cDNA Synthesis Kit, Takara, cat. # 634928). Sequencing libraries were prepared using the Illumina TruSeq protocol and sequenced on an Illumina MiSeq using 250 bp paired end reads ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Full-length RNA-seq libraries from CM and skin macrophages were prepared from ∼3 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) followed by Nextera XT protocol (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were purified using a gel extraction kit (Vazyme, China) and cloned into a pMD-18T vector (Takara, Japan).
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bioRxiv - Cell Biology 2022Quote: ... The gene encoding TERT and mVenus was inserted into pSBbi vector using the In-Fusion cloning kit (Takara Bio, Shiga, Japan). The R3E/R6E mutation was introduced using the KOD mutagenesis kit (Toyobo ...
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bioRxiv - Molecular Biology 2020Quote: ... the genome of Pv11 cells and the clonal cell lines were extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using the following primer set ...
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bioRxiv - Molecular Biology 2020Quote: ... Single cell polyA[+] RNA-seq on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (Takara, 635026) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: cDNA synthesis and amplification was performed using a modified protocol for the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa, Japan) with specific primers (Table S2) ...
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bioRxiv - Genomics 2020Quote: ... RNA-seq library preparation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico (Takara Bio, Mountain View, CA) prior to 100 bp paired-end sequencing ...
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bioRxiv - Genomics 2020Quote: ... Full-length cDNAs were generated on the Fluidigm C1 system using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech, 635026) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Transcripts rising from the identified TSS were determined using the SMARTer Rapid amplification of cDNA ends (RACE) 5’/3’ kit (Takara Bio) in accordance with the manufacturer’s instructions for 3’ RACE ...
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bioRxiv - Genomics 2020Quote: ... and the resulting DNase I-treated RNA (~2 ng) was processed for sequencing by using a SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene expression in the cDNA samples was measured by one-step qRT-PCR using a One Step PrimeScript RT-PCR Kit (Perfect Real Time) according to the manufacturer’s specifications (Takara, Dalian, China). Quantitative real-time PCR was performed on the CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Biochemistry 2020Quote: ... and ligation was used to introduce the synthetic gene in pTHT2031S In-Fusion® HD cloning kit (Takara Bio, Kusatsu, Japan). Sequencing was achieved by Genwiz (Genwiz ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Genetics 2020Quote: ... that contained both of the transgenes was subcloned to a piggyBac vector using the In-Fusion® HD Cloning Kit from Takara with the primers pNE3_UAS_to_pBac.F ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
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bioRxiv - Developmental Biology 2021Quote: Full-length single-cell RNA-seq libraries were prepared using the SMART-Seq v5 Ultra Low Input RNA (SMARTer) Kit for Sequencing (Takara Bio). All reactions were downscaled to one-quarter of the original protocol and performed following thermal cycling manufacturer’s conditions ...
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bioRxiv - Immunology 2021Quote: ... the isolated RNAs were subjected to first-strand cDNA synthesis using (5’-GTCGTATCCAGTGCAGGGTCCGAGGTCACTG GATACGACATACAACA-3’) by PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Japan). After that ...
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bioRxiv - Neuroscience 2020Quote: ... pAAV backbone was cut with restriction enzymes KpnI-HF and AgeI-HF to open the backbone and oG-EGFP fragment was reinserted together with the TVA66T fragment using infusion cloning with an In-Fusion HD cloning kit (Takara bio). The plasmid was packaged into AAV8 by the SALK Viral Vector Core ...
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bioRxiv - Developmental Biology 2021Quote: 1000 FACS-purified cells from TgBAC(tcf21:H2B-Dendra2)ox182 larvae were processed using the SMART-SeqTm v4 UltraTm Low Input RNA Kit for Sequencing (Takara Clontech). Samples were lysed and poly-adenylated RNA reverse transcribed via SMARTScribe Reverse Transcriptase ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized and amplified from 100 pg–300 pg of input RNA using SMART-seqTMv4 Ultra Low input RNA kit (Takara Clontech). Sequencing libraries were prepared using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was generated from 10 ng of RNA using the SMART-Seq v4 UltraLow Input RNA Prep kit (Takara Bio Inc) per manufacturer’s suggestions ...
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bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples were prepared as RNA-seq libraries with the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input (for 250 pg – 100 ng input) (Takara Bio) with RiboZero depletion ...
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bioRxiv - Biochemistry 2020Quote: ... The target 23-mer sequences for sgRNA used were 5’-TTTACCTTGATAGGTGGTAGTGG - 3’and 5’-ATAAAGAGAGGCTTCTCGGGAGG - 3’ and synthesized using Guide-it™ sgRNA In Vitro Transcription Kit (TaKaRa) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and cDNA was generated using the RNA to cDNA EcoDry™ Premix Oligo dT kit (Takara Bio USA, Moutain View, CA). Rin was PCR amplified using primers listed and described in Table S2 and cloned into the HindIII and EcoRI restriction sites of pAc5.1-MCS-(Gly4Ser)3-mCherry to generate the final pAc5.1:Rin-(Gly4Ser)3-mCherry vector.
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bioRxiv - Immunology 2020Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... three types of the GPR151 CDS-related inserts were sub-cloned into FUGW vector using In-Fusion Cloning kit (Takara, 638910). For control experimental sets ...
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bioRxiv - Neuroscience 2021Quote: ... The cloning reaction for pSPE3::H2B-GCaMp6s was performed using linearised PCR products and the In-Fusion HD Cloning Kit (Takara Bio). H2B-GCaMP6s mRNA was produced and precipitated using the mMESSAGE mMACHINE T3 kit (Life Technologies ...