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Citations for Takara Bio :
3601 - 3650 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cDNA amplification was performed using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TAKARA bio) and sequenced using NovaSeq SP flow cell with 50PE reads by Edinburgh Genomics.
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared from 10 ng ChIP DNA using the Thruplex DNA-Seq library prep kit (Takara Bio) and 16 PCR cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was eluted into TE buffer (20mM Tris pH 7.5, 1mM EDTA) followed by library preparation using SMARTer smRNA-Seq Kit (Takara), beginning with reverse transcription (custom RT primer:CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCT CTTCCGATCTTTTTTTTTTTTTTT ...
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bioRxiv - Bioengineering 2024Quote: The reverse transcription program was completed according to the protocol of the PrimeScript™ RT Reagent Kit (Takara, RR047A), with an RNA input amount of 0.5 micrograms per sample ...
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bioRxiv - Biochemistry 2024Quote: ... and converted to first-strand cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Specific binders based on phage ELISA results were sequenced at the University of Chicago Comprehensive Cancer Center DNA Sequencing facility and unique clones were then sub-cloned into the Fab expression vector RH2.2 (kind gift of S. Sidhu) using the In-Fusion Cloning kit (Takara). Successful cloning was verified by DNA sequencing ...
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DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptidesbioRxiv - Biophysics 2024Quote: ... All other constructs in this work were made using the In-Fusion® HD Cloning Kit (Takara Bio USA).
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bioRxiv - Cell Biology 2024Quote: ... The plasmid was then transfected into HEK293T cells using the Lenti-XTM Packaging Single Shots kit (Takara Bio # 631278) according to kit instructions ...
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bioRxiv - Biochemistry 2024Quote: Specific binders based on phage ELISA results were sequenced at the University of Chicago Comprehensive Cancer Center DNA Sequencing Facility and unique clones were then sub-cloned into the Fab expression vector RH2.2 (kind gift of S. Sidhu) using the In-Fusion Cloning kit (Takara). Successful cloning was verified by DNA sequencing ...
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Developmental functions of rapgef1b in neural crest specification and presomitic mesoderm patterningbioRxiv - Developmental Biology 2024Quote: ... The total RNA was used to prepare cDNA using the PrimeScript 1st strand cDNA synthesis kit (TaKaRa, Cat. # 6110A). Sequence-specific primers were used to amplify the specific gene fragment and cloned into the pGEMT easy vector and the sequence was verified ...
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bioRxiv - Developmental Biology 2024Quote: The total RNA was used to prepare cDNA using the PrimeScript 1st strand cDNA synthesis kit (TaKaRa, Cat. # 6110A). Sequence-specific primers were used to amplify the specific gene fragment and cloned into the pGEMT easy vector and the sequence was verified ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: RNA-seq libraries (used for calculating translation efficiencies) were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara), with 50 ng total RNA as input ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 5 μg of RNA were reverse transcribed and amplified using One Step SYBR Prime-Script PLUS RT-PCR Kit (TaKaRa) and the Thermal Cycler Dice instrument (TaKaRa ...
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bioRxiv - Molecular Biology 2020Quote: ... One µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... An RNA library was prepared by JHU Deep Sequencing Core facility beginning with cDNA construction using the SMARTer pico cDNA Synthesis Kit (TaKaRa), followed by ribosomal depletion and library construction using the Illumina TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara, cat.631235). Lentivirus with MOI of 10 was used for transduction of MOVAS cells ...
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bioRxiv - Genetics 2021Quote: ... The volume was brought to 11.5 μl and libraries were processed using the SMART-Seq HT library preparation kit (634456, Takara Bio) as described (Blackburn et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time quantitative PCR was performed in triplicate using the SYBR Premix Ex Taq (Tli RNase H Plus) kit (Takara). The expression of TBP was used for the normalization of mRNA expression ...
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bioRxiv - Genetics 2021Quote: ... and then RT-qPCR was performed using a One Step SYBR PrimeScript PLUS RT-PCR kit (Takara Bio, Shiga, Japan) and Applied Biosystems ABI Prism 7000 Sequence Detection System ...
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bioRxiv - Molecular Biology 2020Quote: ... a fluorescent quantitative PCR was carried out in accordance with the manufacturer’s instructions of a SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) kit (Takara). The primers were synthesized by RiboBio Company and were shown in Table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... The purified PCR products were cloned into the expression vector pFO4 predigested with EcoRI and BamHI using the In-Fusion HD Cloning kit (TaKaRa). This plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA libraries were prepared using 1-5 ng of starting material using the SMARTer ThruPLEX DNA-Seq kit (Takara; R400674) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 4 kb of the 3’ flanking region of MpBLD10 was inserted into the SmaI site of the pENTR/D-TOPO vector containing proMpBLD10 by using an In-Fusion HD cloning kit (Clontech). A silent mutation was introduced into the PAM site by inverse PCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The CDS of monomeric Citrine was introduced into the SmaI site of the pENTR/D-TOPO vector containing the MpBLD10 genomic fragment by using an In-Fusion HD cloning kit (Clontech). The chimeric sequence was introduced into pMpGWB301 (Ishizaki et al ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA purified from snap-frozen larvae using the Trizol method and used as template to synthesize random-primed cDNA using the Primescript cDNA synthesis kit (TaKaRa). Relative gene expression levels were measured by qRT-PCR ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR analyses were carried out with KAPA SYBR FAST qPCR kits using a Thermal Cycler Dice Real Time System (TaKaRa) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA libraries were generated using Takara’s SMART-Seq v4 Low Input RNA Kit for Sequencing (Takara, Mountain View, California, USA) for cDNA synthesis and the Illumina NexteraXT DNA Library Preparation (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... and we immediately used these cells for reverse-transcription and cDNA amplification with a Smart-Seq HT kit (Cat# 634437, Takara). The cDNAs (1 ng each ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA, 7246).
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the BamHI/XhoI site of pBluescript KS– using an In-Fusion HD cloning kit (Takara), yielding pOsSOG1_7A ...
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bioRxiv - Molecular Biology 2022Quote: About 1μg of RNAs were reverse transcribed into cDNAs using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified fragments were cloned into the TRV vector (pTRV2) (Lu et al., 2003) using the In-Fusion HD cloning kit (Clontech). Agrobacterium strains containing desired pTRV2 constructs were co-infiltrated with strains carrying pTRV1 at OD600=0.5 into N ...
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bioRxiv - Microbiology 2022Quote: ... non-structural genes were PCR amplified in three fragments from the pRepDVRluc plasmid [37] and all fragments were inserted into the PUb-MCS-2A-Puro plasmid [64] using In-Fusion cloning kit (Takara) to generate the PUb-NS(Ø ...
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bioRxiv - Developmental Biology 2022Quote: ... Three nanograms of total RNA were used for amplification using the SMART-Seq V4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s recommendations (10 PCR cycles were performed) ...
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bioRxiv - Neuroscience 2021Quote: ... Groups of 6-10 cells were dispensed from the micropipette into 1 µl ice-cold 10x reaction buffer (SMART-Seq v4 kit, Takara) and flash-frozen before library preparation ...
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bioRxiv - Cell Biology 2022Quote: ... LC NLS deletion (Δ417-422) was generated by KOD One PCR amplification and the In-Fusion HD Cloning Kit (Clontech). The NLS of SUN2 (KDSPLRTLKRKSSNMKRL ...
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bioRxiv - Developmental Biology 2022Quote: ... 250–2000 pg of total RNA was loaded as a template for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) with template switching technology ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa) with each specific primer (Table S2).
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bioRxiv - Genomics 2020Quote: ... 0.8μg of Total RNA was utilized as template for RT with random hexamer primers using PrimeScript RT reagent Kit (Takara). qRT-PCR was performed with respective gene-specific primers (PD GFD ...
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bioRxiv - Immunology 2021Quote: NGS transcriptome libraries were generated from 73 healthy baseline samples and 95 convalescence samples using the SMART-Seq v4 Ultra low Input RNA kit (Takara), an optimized version of the SMART-Seq2 method ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from the RNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Bio Inc, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... both input and IP samples were used for library construction with the SMARTer Stranded Total RNA-seq Kit v2 (634413, Takara), and sequenced by Illumina HiSeq X Ten to produce 150 bp paired-end reads.
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bioRxiv - Pathology 2020Quote: ... The PCR assay was conducted as described previously9 and the complete genome termini was determined using the Takara SMARTer RACE 5’/3’ kit (TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were cloned into pBAD28 vector (ATCC® 87400™) by using In-Fusion HD Cloning kit (Takara) by using primers 7 and 8 to amplify pBAD28 vector (Table S2 ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Pathology 2021Quote: ... Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech, 632165). Results were read on an ABI QuantStudio 6 RT-PCR machine.
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bioRxiv - Systems Biology 2020Quote: We extracted RNA from fixed cells after barcode RNA FISH and sorting using the NucleoSpin total RNA FFPE XS kit (Takara). We performed cell lysis and reverse cross-linking at 50°C for 90 minutes and otherwise followed the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 5′UTR of test genes and EGFP were inserted into pCAG-FLAG-N1 plasmid 48 using In-Fusion HD Cloning Kit (TAKARA).