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Citations for Takara Bio :
3551 - 3600 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... total RNA (1.9 mg) was used to synthesize first-strand cDNA using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was generated from a Katnal1 conditional KO targeting construct using the Guide-it Long ssDNA Strandase Kit (Takara, 632645). The following oligonucleotides were used to generate a PCR product from which the ssDNA was generated ...
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bioRxiv - Microbiology 2022Quote: ... Isolated RNA or serial ten-fold dilutions of RNA standards for the ORF1ab amplicon (ranging from 2.25×10^6 to 250 copies/rxn) were reverse transcribed using the Takara Prime Script RT kit (Takara) using poly(A ...
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bioRxiv - Developmental Biology 2023Quote: ... Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Kusatsu, Japan) by replacing AUG with AAG at Met1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA amplification was performed using the SMART-Seq® v4 Ultra® low input RNA kit (Takara, cat. # 634891) with sixteen cycles of PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: ... Synthesis of cDNA and library preparation were performed using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech) and the Ion Plus Fragment Library Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplified and subcloned into a pCG-strep expression construct using the In-Fusion® HD Cloning Kit (Takara) according to the manufacture’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Full-length cDNA was synthesized with 1.0 μg RNA from Diphasiastrum complanatum using the SMARTer PCR cDNA Synthesis kit (Clontech). The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was then extracted from the tissues following the guidelines of the RNAiso Plus kit (Takara, Shiga, Japan). Subsequently ...
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bioRxiv - Genomics 2024Quote: ... the RNA samples were reverse-transcribed to cDNA using the SMART-Seq HT Kit (Takara Bio USA, CA, USA) following the manufacturer’s guidelines ...
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bioRxiv - Genetics 2024Quote: ... and total RNA was extracted and subsequently used to synthesize cDNA using the TaKaRa RNA PCRTM Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight gDNA extractions for samples M20 and M29 were generated using the NucleoBond HMW DNA kit (740160.20) from Takara Bio USA ...
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bioRxiv - Genomics 2024Quote: ... The subsequent steps for library preparation were carried out with the DNA Thruplex kit (Takara, Cat. #R400674, Cat. #R400665) following the manufacturer’s protocols.
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bioRxiv - Cell Biology 2024Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by an ELISA kit (eBiosciences ...
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bioRxiv - Biophysics 2024Quote: ... The resulting product was separated on a 0.75 % agarose gel and the 11.6-kb dsRNA was excised and purified using Agarose Gel DNA Extraction Kit (Takara).
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bioRxiv - Systems Biology 2024Quote: The pool of duplets was ligated to each of the barcoded promoter vectors using Takara ligation kit version 2.1 (#6022; Takara). Ligation products were purified using magnetic bead purification and 2 µl of the ligation were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Genetics 2024Quote: ... total RNA libraries were prepared by using SMART-Seq v4 Ultra® Low Input RNA Kit for Sequencing (Clontech) according to the manufacture’s instruction and analyzed by Illumina HiSeq 2500 system (Illumina) ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara), and a Light Cycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Immunology 2024Quote: ... Total RNA Sequencing libraries were prepared with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: All DNA constructs used in this study were generated by using In-Fusion HD Cloning kit (Takara Bio Inc.). Plasmid mEmerald-N1 was a gift from Michael Davidson (Addgene plasmid #53976) ...
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bioRxiv - Plant Biology 2024Quote: ... 5’RACE was then conducted using the purified mRNA and the SMART RACE cDNA amplification kit following the manufacturer’s instructions (Clontech, Takara Bio Europe ...
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bioRxiv - Plant Biology 2024Quote: ... 5’RACE was then conducted using the purified mRNA and the SMART RACE cDNA amplification kit following the manufacturer’s instructions (Clontech, Takara Bio Europe ...
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bioRxiv - Genomics 2024Quote: Full length cDNA was obtained using the SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Clontech, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Purified RNA samples were converted to cDNA using the SMART-seq v4 Ultra Low Input RNA Kit (Takara Bio), and cDNA libraries were generated with the Nextera XT DNA Library Preparation Kit (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized using polydT primers and a SMARTer PCR cDNA Synthesis Kit (Cat# 634926, TaKaRa Bio, Shiga, Japan), and double-stranded cDNA was synthesized via downstream large-scale PCRs using PrimeSTAR GXL DNA Polymerase (Cat# R050A ...
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bioRxiv - Genomics 2024Quote: ... The digested and purified reporter pool was then ligated into 80 ng of the linearized entry vector using Takara ligation kit v1.0 (#6021; Takara) at a 1:3 (vector:insert ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of RNA were retrotranscribed into cDNA by means of the PrimeScript RT reagent Kit (Takara Bio Inc), followed by amplification using SYBR Premix Ex Taq (Takara ...
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bioRxiv - Bioengineering 2024Quote: The reverse transcription program was completed according to the protocol of the PrimeScript™ RT Reagent Kit (Takara, RR047A), with an RNA input amount of 0.5 micrograms per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from equal amount of RNA by using the PrimeScript RT Reagent kit (Takara, cat. no. RR037). RT-qPCR was performed using an ABI Prism 7500 fast system (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... All cloning steps were performed using the CloneAmp HiFi PCR premix and the In-Fusion HD Cloning Kit (Takara). The resulting plasmids were verified by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Immunology 2024Quote: ... 1 µg of total RNA was reverse transcribed using the PrimeScript 1st strand cDNA Synthesis kit (Takara, catalog 6110A). The resulting cDNA was employed for qRT-PCR using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... cDNA amplification was performed using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TAKARA bio) and sequenced using NovaSeq SP flow cell with 50PE reads by Edinburgh Genomics.
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bioRxiv - Molecular Biology 2024Quote: ... The ligated RNA was then subjected to TaqMan qPCR using the One Step PrimeScript RT-PCR Kit (Takara Bio), with 200 nM of a TaqMan probe targeting the boundary of the target RNA and the 3′-AD ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to RT with the use of a TaKaRa PrimeScript II 1st Stand cDNA Synthesis Kit (Takara Bio). The resulting cDNA was subjected to real-time PCR analysis with Fast SYBR Green Master Mix (3485612 ...
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bioRxiv - Neuroscience 2024Quote: ... 1µg of total RNA was mixed with a total volume of 10µl of cDNA synthesis kit (TaKaRa 6110A, Tokyo) consisting of dNTP mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... LTR amplicons or gBlocks™ Gene Fragments were mixed with the pLd2G backbone (gLTR_OUT) and genomic (gLTR_IN) amplicons and joined in a single reaction using In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Bioengineering 2024Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio,740609.250). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... 90 μl of RNase-free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara) and primers for the nucleocapsid (N ...
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bioRxiv - Zoology 2024Quote: ... Sequencing library fragments in the range of 400–1,000 bp were cut and purified with a NucleoSpin Gel and PCR Clean-up kit (Clontech) and sequenced using a NextSeq500 sequencer with HighOutputMode 75 cycles kit (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... The incubated DNA samples were purified with the NucleoSpin Gel and PCR Clean-up kit (#740609.250; Takara Bio Inc.). The purified DNA samples were used to prepare the library ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing was performed using SMARTer chemistry (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, Clontech 634835/634935) (three retinae ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from homogenized lung tissues was reverse transcribed into cDNA using MiniBEST Universal RNA Extraction Kit (Takara) and PrimeScriptTM RT reagent kit (Takara) ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of total RNA was used for synthesis of cDNA using PrimeScriptTM RT reagent Kit (Takara, Tokyo, Japan). For miRNA expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... and all gene cloning operations were performed using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). A conjugated pZJD29a vector [65] was employed to clone 500-bp upstream and downstream regions of the target gene ...
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bioRxiv - Neuroscience 2024Quote: ... Endotoxin-free DNA was obtained through a commercial midiprep plasmid purification kit (NucleoBond Xtra Midi EF, Takara Bio USA).
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bioRxiv - Neuroscience 2024Quote: ... Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v3-Pico Input Mammalian (Takara Bio Inc., Japan), which features integration of unique molecular identifiers (UMIs ...
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bioRxiv - Neuroscience 2024Quote: ... Library construction is performed based on manufacturer’s recommendation for the SMARTer smRNA library preparation kit (Takara Bio USA Inc). Final library quantity was measured by KAPA SYBR® FAST qPCR and library quality evaluated by TapeStation D1000 ScreenTape (Agilent Technologies) ...